ISOLATION AND PURIFICATION OF METHANE IVf ONOOXYGENASE FROM METHYLOMONAS SPECIES GYJ3

A three—component enzyme system that catalyzes in vivo the oxidation of CH_4 to CH_3OH has been purified with high specific activity from an unusual type I methanotroph through the use of stabilizing reagents.

FORMATION OF PROPYLENE OXIDE BY METHYLOMONAS GYJ3 IN A GAS-SOLID BIOREACTOR

Present paper deals with propylene oxide production from propylene utilizing Methylomonas sp.GYJ3 immobilized by adsorption or gel entrapment in a continuous gas flow packed bed bioreactor.The regeneration of biocatalyst in situ is al- so discussed.

微生物法转化甲烷合成甲醇的研究

对实验室筛选的一株甲烷氧化菌株Methylomonas.QJ16转化甲烷生成甲醇的液体发酵条件进行了研究,得出最佳参数为:1.50 g/L KNO3,0.80 g/L NH4Cl,0.25 g/L KH2PO4,0.74 g/L Na2HPO4,0.50 g/L MgSO4.7H2O,0.01 g/L EDTA,0.2 g/LCaCl2.2H2O,微量元素1mL;初始pH 7.0,通入气体V(甲烷)/V(空气)=3/7,接种体积分数8%,120mL瓶装液量20mL,发酵时间6d,温度32℃,摇床转速180 r/min,在该条件下批式反应的甲醇浓度约为2 mmol/L。