Arginine decarboxylase （ADC） plays an important role in polyamine biosynthesis in plants. In order to study the structure and expression ofADC gene in mango, an arginine decarboxylase gene ADC, named as MiADC, was c...Arginine decarboxylase （ADC） plays an important role in polyamine biosynthesis in plants. In order to study the structure and expression ofADC gene in mango, an arginine decarboxylase gene ADC, named as MiADC, was cloned and isolated from the tender leaves of mango using RACE technology, and the expression of MiADC was analyzed by qRT-PCR based on the cDNA sequence of MiADC gene in this study. The results showed that the full length of MiADC was 3 089 bp containing a 575 bp 5＇-UTR, a 2 178 bp ORF with file initial codon and the terminal codon, a 311 bp 3＇-UTR and a 25 bp poly （A）; and file MiADC had no intron. The homologous analysis in the NCBI demonstrated that the sequence of MiADC had the highest similarity at 78% to Poncirus trifoliata arginine decarboxylase gene （HQ008237.1）, and the prediction protein also had the highest homology at 78% level with Poncirus trifoliata arginine decarboxylase （AEE99192.1） . Phylogenetic tree analysis revealed that the MiADC in mango was clustered in the same clade as the arginine decarboxylase from Poncirus trifoliate. Expression analysis showed that the MiADC gene was expressed in roots, stems, leaves, flowers, fruitlet, ripe fruit, and the transcriptional level of the cloned gene was affected obviously by low temperature, which proved that the MiADC gene may be a low temperature stress responding gene.展开更多
文摘Arginine decarboxylase （ADC） plays an important role in polyamine biosynthesis in plants. In order to study the structure and expression ofADC gene in mango, an arginine decarboxylase gene ADC, named as MiADC, was cloned and isolated from the tender leaves of mango using RACE technology, and the expression of MiADC was analyzed by qRT-PCR based on the cDNA sequence of MiADC gene in this study. The results showed that the full length of MiADC was 3 089 bp containing a 575 bp 5＇-UTR, a 2 178 bp ORF with file initial codon and the terminal codon, a 311 bp 3＇-UTR and a 25 bp poly （A）; and file MiADC had no intron. The homologous analysis in the NCBI demonstrated that the sequence of MiADC had the highest similarity at 78% to Poncirus trifoliata arginine decarboxylase gene （HQ008237.1）, and the prediction protein also had the highest homology at 78% level with Poncirus trifoliata arginine decarboxylase （AEE99192.1） . Phylogenetic tree analysis revealed that the MiADC in mango was clustered in the same clade as the arginine decarboxylase from Poncirus trifoliate. Expression analysis showed that the MiADC gene was expressed in roots, stems, leaves, flowers, fruitlet, ripe fruit, and the transcriptional level of the cloned gene was affected obviously by low temperature, which proved that the MiADC gene may be a low temperature stress responding gene.
