miR-135 is a highly conserved miRNA in mammals and includes miR-135a and miR-135b.Recent studies have shown that miR-135b is a key regulatory factor in cardio-cerebrovascular diseases.It is involved in regulating the ...miR-135 is a highly conserved miRNA in mammals and includes miR-135a and miR-135b.Recent studies have shown that miR-135b is a key regulatory factor in cardio-cerebrovascular diseases.It is involved in regulating the pathological process of myocardial infarction,myocardial ischemia/reperfusion injury,cardiac hypertrophy,atrial fibrillation,diabetic cardiomyopathy,atherosclerosis,pulmonary hyperten-sion,cerebral ischemia/reperfusion injury,Parkinson's disease,and Alzheimer's disease.Obviously,miR-135b is an emerging player in cardio-cerebrovascular diseases and is expected to be an important target for the treatment of cardio-cerebrovascular diseases.However,the crucial role of miR-135b in cardio-cerebrovascular diseases and its underlying mechanism of action has not been reviewed.Therefore,in this review,we aimed to comprehensively summarize the role of miR-135b and the signaling pathway mediated by miR-135b in cardio-cerebrovascular diseases.Drugs targeting miR-135b for the treatment of diseases and related patents,highlighting the importance of this target and its utility as a therapeutic target for cardio-cerebrovascular diseases,have been discussed.展开更多
目的探讨miR-135b在子宫内膜癌中的表达及对子宫内膜癌细胞增殖的作用的机制。方法运用RT-PCR的方法检测22对子宫内膜癌组织及相应的癌旁组织中的miR-135b和FOXO1的表达;运用RT-PCR的方法检测JEC、Ishikawa、HEC-1-B、RL-952这4种子宫...目的探讨miR-135b在子宫内膜癌中的表达及对子宫内膜癌细胞增殖的作用的机制。方法运用RT-PCR的方法检测22对子宫内膜癌组织及相应的癌旁组织中的miR-135b和FOXO1的表达;运用RT-PCR的方法检测JEC、Ishikawa、HEC-1-B、RL-952这4种子宫内膜癌细胞株中miR-135b和FOXO1的表达。分别转染miR-135b的模拟物抑制物于子宫内膜癌细胞株,通过RT-PCR检测转染的效果,通过MTT增殖试验检测对子宫内膜癌细胞增殖的影响。通过RT-PCR的方法检测FOXO1 m RNA的变化,Western blotting检测在FOXO1蛋白的变化。结果 miR-135b在子宫内膜癌组织中较对应的癌旁组织表达增高(P<0.05),FOXO1在子宫内膜癌组织中较对应的癌旁组织表达降低(P<0.05)。荧光定量PCR显示子宫内膜癌细胞中miR-135b与FOXO1表达趋势成负相关。miR-135b能促进子宫内膜癌细胞增殖(P<0.05)。上调miR-135b能够降低FOXO1 m RNA和蛋白的表达(P<0.05),下调miR-135b能够提高FOXO1 m RNA和蛋白的表达(P<0.05)。结论 miR-135b在子宫内膜癌的发生发展中发挥重要的作用,其部分分子机制可能是通过调控靶基因FOXO1而发挥作用。展开更多
MicroRNAs(miRNAs) have been widely identified in porcine testicular tissues and implicated as crucial regulators of proliferation, apoptosis, and differentiation in porcine spermatogenesis related cells. However, the ...MicroRNAs(miRNAs) have been widely identified in porcine testicular tissues and implicated as crucial regulators of proliferation, apoptosis, and differentiation in porcine spermatogenesis related cells. However, the function roles of most of the miRNAs that have been identified in Sertoli cells are poorly understood. In the present study, six experiments were conducted to study the regulatory role of miR-10b in porcine immature Sertoli cells. In experiment 1, the results showed that the relative mRNA expression level of miR-10b in porcine testicular tissues decreased quadratically(P<0.001) with increasing age, while the relative mRNA expression level of DAZAP1 gene increased(P<0.001). In addition, the mRNA expression of miR-10b was negatively(P<0.01) correlated with DAZAP1 mRNA expression(r=–0.550). In experiment 2, the results from the bioinformatic analysis and a luciferase reporter assay demonstrated that miR-10b directly targeted the DAZAP1 gene in porcine immature Sertoli cells. DAZAP1 mRNA and protein expressions were both regulated(P<0.05) by miR-10b. In experiments 3 to 5, the over-expression of miR-10b or the siRNA-mediated knockdown of the DAZAP1 gene promoted(P<0.05) porcine immature Sertoli cell proliferation, as determined by the Cell Counting Kit-8(CCK-8) assay and the 5-Ethynyl-2′-deoxyuridine(EdU) assay. However, an annexin V-FITC/PI staining assay and the expression of cell survival-related genes indicated that over-expression of miR-10b or knockdown of DAZAP1 had no effect(P>0.05) on porcine immature Sertoli cell apoptosis. In experiment 6, the co-transfection treatment results showed that miR-10b promoted(P<0.05) porcine immature Sertoli cell proliferation by targeting DAZAP1 gene. Overall, these experiments demonstrated that miR-10b promotes porcine immature Sertoli cell proliferation by targeting the DAZAP1 gene.展开更多
基金This work was supported by the Natural Science Foundation of Shandong Province,China(Grant No.:ZR2023QH007)the Natural Science Foundation of Qingdao Municipality,China(Grant No.:23-2-1-135-zyyd-jch)+1 种基金the Shandong Postdoctoral Science Foundation,China(Grant No.:SDBX2023049)the China Postdoctoral Science Foundation(Grant No.:2023M731852).
文摘miR-135 is a highly conserved miRNA in mammals and includes miR-135a and miR-135b.Recent studies have shown that miR-135b is a key regulatory factor in cardio-cerebrovascular diseases.It is involved in regulating the pathological process of myocardial infarction,myocardial ischemia/reperfusion injury,cardiac hypertrophy,atrial fibrillation,diabetic cardiomyopathy,atherosclerosis,pulmonary hyperten-sion,cerebral ischemia/reperfusion injury,Parkinson's disease,and Alzheimer's disease.Obviously,miR-135b is an emerging player in cardio-cerebrovascular diseases and is expected to be an important target for the treatment of cardio-cerebrovascular diseases.However,the crucial role of miR-135b in cardio-cerebrovascular diseases and its underlying mechanism of action has not been reviewed.Therefore,in this review,we aimed to comprehensively summarize the role of miR-135b and the signaling pathway mediated by miR-135b in cardio-cerebrovascular diseases.Drugs targeting miR-135b for the treatment of diseases and related patents,highlighting the importance of this target and its utility as a therapeutic target for cardio-cerebrovascular diseases,have been discussed.
文摘目的探讨miR-135b在子宫内膜癌中的表达及对子宫内膜癌细胞增殖的作用的机制。方法运用RT-PCR的方法检测22对子宫内膜癌组织及相应的癌旁组织中的miR-135b和FOXO1的表达;运用RT-PCR的方法检测JEC、Ishikawa、HEC-1-B、RL-952这4种子宫内膜癌细胞株中miR-135b和FOXO1的表达。分别转染miR-135b的模拟物抑制物于子宫内膜癌细胞株,通过RT-PCR检测转染的效果,通过MTT增殖试验检测对子宫内膜癌细胞增殖的影响。通过RT-PCR的方法检测FOXO1 m RNA的变化,Western blotting检测在FOXO1蛋白的变化。结果 miR-135b在子宫内膜癌组织中较对应的癌旁组织表达增高(P<0.05),FOXO1在子宫内膜癌组织中较对应的癌旁组织表达降低(P<0.05)。荧光定量PCR显示子宫内膜癌细胞中miR-135b与FOXO1表达趋势成负相关。miR-135b能促进子宫内膜癌细胞增殖(P<0.05)。上调miR-135b能够降低FOXO1 m RNA和蛋白的表达(P<0.05),下调miR-135b能够提高FOXO1 m RNA和蛋白的表达(P<0.05)。结论 miR-135b在子宫内膜癌的发生发展中发挥重要的作用,其部分分子机制可能是通过调控靶基因FOXO1而发挥作用。
基金financially supported by the earmarked fund for China Agriculture Research System (CARS-36)the Hunan Provincial Natural Science Foundation of China (2018JJ2176 and 2018JJ3219)
文摘MicroRNAs(miRNAs) have been widely identified in porcine testicular tissues and implicated as crucial regulators of proliferation, apoptosis, and differentiation in porcine spermatogenesis related cells. However, the function roles of most of the miRNAs that have been identified in Sertoli cells are poorly understood. In the present study, six experiments were conducted to study the regulatory role of miR-10b in porcine immature Sertoli cells. In experiment 1, the results showed that the relative mRNA expression level of miR-10b in porcine testicular tissues decreased quadratically(P<0.001) with increasing age, while the relative mRNA expression level of DAZAP1 gene increased(P<0.001). In addition, the mRNA expression of miR-10b was negatively(P<0.01) correlated with DAZAP1 mRNA expression(r=–0.550). In experiment 2, the results from the bioinformatic analysis and a luciferase reporter assay demonstrated that miR-10b directly targeted the DAZAP1 gene in porcine immature Sertoli cells. DAZAP1 mRNA and protein expressions were both regulated(P<0.05) by miR-10b. In experiments 3 to 5, the over-expression of miR-10b or the siRNA-mediated knockdown of the DAZAP1 gene promoted(P<0.05) porcine immature Sertoli cell proliferation, as determined by the Cell Counting Kit-8(CCK-8) assay and the 5-Ethynyl-2′-deoxyuridine(EdU) assay. However, an annexin V-FITC/PI staining assay and the expression of cell survival-related genes indicated that over-expression of miR-10b or knockdown of DAZAP1 had no effect(P>0.05) on porcine immature Sertoli cell apoptosis. In experiment 6, the co-transfection treatment results showed that miR-10b promoted(P<0.05) porcine immature Sertoli cell proliferation by targeting DAZAP1 gene. Overall, these experiments demonstrated that miR-10b promotes porcine immature Sertoli cell proliferation by targeting the DAZAP1 gene.
