AIM:To investigate the correlation of miR-193a-5p with lymph node metastasis and postoperative survival of colorectal cancer(CRC)patients.METHODS:A total of 304 formalin-fixed,paraffinembedded specimens(69 paired canc...AIM:To investigate the correlation of miR-193a-5p with lymph node metastasis and postoperative survival of colorectal cancer(CRC)patients.METHODS:A total of 304 formalin-fixed,paraffinembedded specimens(69 paired cancer and normal tissues,55 primary tumors of stageⅢCRC and matched lymph nodes,and 56 primary tumors of stageⅡCRC)were included in this study.The relative expression lev-els of miR-193a-5p in the normal mucosa,primary cancer,and metastatic lymph node lesions were measured by quantitative real-time reverse transcriptase polymerase chain reaction.We evaluated the association of its expression with colorectal cancer lymph node metastasis,clinicopathological factors,and patient survival.RESULTS:The relative expression level of miR-193a-5p was significantly lower in CRC tissues than in the normal mucosa(P=0.0060).The expression levels of miR-193a-5p were lower in primary CRC tissues with lymph node metastases than in those without metastases(P=0.0006),and decreased expression of miR-193a-5p correlated with advanced lymph node metastatic stage(P=0.0007).Kaplan-Meier analysis showed that patients with low miR-193a-5p expression had decreased disease-free survival(DFS)(P=0.0026)and poor overall survival(OS)(P=0.0003).Interestingly,for the group of patients with lymph node metastases,miR-193a-5p expression was also related to survival.Patients with low miR-193a-5p expression had decreased DFS(P=0.0262)and poor OS(P=0.0230).Moreover,multivariate analysis indicated that downregulation of miR-193a-5p was an independent predictor of poor OS.CONCLUSION:Downregulation of miR-193a-5p correlates with lymph node metastasis and poor survival of CRC.miR-193a-5p may be a useful biomarker for CRC diagnosis,metastasis and prognosis prediction.展开更多
基金Supported by National Natural Science Foundation of China,No.30872467,No.81030040,No.81201965 and No.81372593Beijing Natural Science Foundation,No.7132052the National High Technology Research and Development Program of China(863 Program),No.2012AA02A506
文摘AIM:To investigate the correlation of miR-193a-5p with lymph node metastasis and postoperative survival of colorectal cancer(CRC)patients.METHODS:A total of 304 formalin-fixed,paraffinembedded specimens(69 paired cancer and normal tissues,55 primary tumors of stageⅢCRC and matched lymph nodes,and 56 primary tumors of stageⅡCRC)were included in this study.The relative expression lev-els of miR-193a-5p in the normal mucosa,primary cancer,and metastatic lymph node lesions were measured by quantitative real-time reverse transcriptase polymerase chain reaction.We evaluated the association of its expression with colorectal cancer lymph node metastasis,clinicopathological factors,and patient survival.RESULTS:The relative expression level of miR-193a-5p was significantly lower in CRC tissues than in the normal mucosa(P=0.0060).The expression levels of miR-193a-5p were lower in primary CRC tissues with lymph node metastases than in those without metastases(P=0.0006),and decreased expression of miR-193a-5p correlated with advanced lymph node metastatic stage(P=0.0007).Kaplan-Meier analysis showed that patients with low miR-193a-5p expression had decreased disease-free survival(DFS)(P=0.0026)and poor overall survival(OS)(P=0.0003).Interestingly,for the group of patients with lymph node metastases,miR-193a-5p expression was also related to survival.Patients with low miR-193a-5p expression had decreased DFS(P=0.0262)and poor OS(P=0.0230).Moreover,multivariate analysis indicated that downregulation of miR-193a-5p was an independent predictor of poor OS.CONCLUSION:Downregulation of miR-193a-5p correlates with lymph node metastasis and poor survival of CRC.miR-193a-5p may be a useful biomarker for CRC diagnosis,metastasis and prognosis prediction.
