[Objectives] To study the protective and recovery effects of Yupingfeng polysaccharides( YPF-P) on spleen tissue structure of acute and chronic immunocompromised mice. [Methods]First,90 mice in the protective group we...[Objectives] To study the protective and recovery effects of Yupingfeng polysaccharides( YPF-P) on spleen tissue structure of acute and chronic immunocompromised mice. [Methods]First,90 mice in the protective group were injected with cyclophosphamide to replicate the chronic immunosuppressive model,and observe the protective effects of different doses of YPF-P on the spleen; 120 mice in the recovery group were injected with cyclophosphamide continuously to replicate the acute immune injury model,to observe the effects of YPF-P on spleen recovery. Besides,YPF-P positive control group was established to observe its effects on the spleen structure and function of normal mice. Testing indicators include spleen morphology and tissue structure,body mass,and spleen index. [Results]In the protective group and the recovery group,the spleen volume of the mice with different YPF-P doses increased significantly,and lymphocytes proliferated,showing obvious structural compensation and repair,and showing a dose-effect relationship. In the positive control group,the spleen function of the normal mice was significantly enhanced,and the spleen lymphoid tissue showed significant orderly proliferation. [Conclusions]Yupingfeng polysaccharides can effectively protect the function and structure of spleen,improve acute and chronic immune damage induced by cyclophosphamide,greatly promote the recovery of spleen structure and function after immune injury,and also can significant enhance the spleen structure and function of normal mice.展开更多
Objective: To study the effect on apoptosis of CD 4 +T、CD 19 +B in spleen of BXSB mice with systemic lupus erythematosus treated with Langchuangjing Granule (LCJG, and to probe into the mechanism of the tr ea...Objective: To study the effect on apoptosis of CD 4 +T、CD 19 +B in spleen of BXSB mice with systemic lupus erythematosus treated with Langchuangjing Granule (LCJG, and to probe into the mechanism of the tr eatment. Methods: The apoptosis was examined by the flow cytometric analysis and immunofluorescence double-staining method. Results: Apoptosis of male BXSB mice speeds up. LCJG can restrain the excessive apoptosis of CD 4 +T and CD 19 +B cells in spleen. Conclusion: LCJG treated systemic lupus erythematosus by restraining the excessive apoptosis of T, B lymphocytes, probably restraining the release of excessive amount of apoptotic DNA fragments, so decreasing abnormal proliferation of B cells and the produce of autoantibodies.展开更多
[Objective] The paper was to discuss regulation and protection effect of Yupingfeng polysaccharide （YPF-P） on immunosuppressive mice. [Method] Taking immunosuppressive mice induced by 80 mg/kg cyclophosphamide as te...[Objective] The paper was to discuss regulation and protection effect of Yupingfeng polysaccharide （YPF-P） on immunosuppressive mice. [Method] Taking immunosuppressive mice induced by 80 mg/kg cyclophosphamide as test animal model, effects of different doses （100,200 and 400 mg/kg） of YPF-P on spleen index, serum IL-2 and IFN-γ levels of mice were studied. [ Result] High dose of YPF-P （400 mg/kg） could improve the spleen index of immunosuppressive mice （P 〈0.05）. YPF-P could enhance serum IL-2 and IFN-γ levels of immunosuppressive mice, and the effects in middle-dose group and low-dose group were better. YPF-P could significantly increase serum IL-2 level of normal mice （ P 〈 0.01 ）, but it had no significant effect on serum IFN-γ level of normal mice. [ Conclusion] The research provides an experimental basis for clinical application of YPF-P.展开更多
Mice were watered with cerous nitrate solution (2, 20, 200 mg·kg-1) according to daily drinking water volume for 50 days and the effects of cerous nitrate on nonspecific immunity in mice were investigated. The re...Mice were watered with cerous nitrate solution (2, 20, 200 mg·kg-1) according to daily drinking water volume for 50 days and the effects of cerous nitrate on nonspecific immunity in mice were investigated. The results showed that the total number of peripheral white blood cell, the clearance rate of charcoal particles, the indices of thymus and spleen were increased on different degrees, and associated with the watering dose, noting that these indices were increased significantly by cerous nitrate at a dose of 20 mg·kg-1 compared with control group. Therefore, it was concluded that the nonspecific immunity could be improved by rare earth Ce in mice.展开更多
基金Supported by Science and Technology Planning Project of Guangdong Province(2016A020210140)Project of Key Laboratory of Preventive Veterinary Medicine for Guangdong Provincial Department of Education(2014KTSPT037)
文摘[Objectives] To study the protective and recovery effects of Yupingfeng polysaccharides( YPF-P) on spleen tissue structure of acute and chronic immunocompromised mice. [Methods]First,90 mice in the protective group were injected with cyclophosphamide to replicate the chronic immunosuppressive model,and observe the protective effects of different doses of YPF-P on the spleen; 120 mice in the recovery group were injected with cyclophosphamide continuously to replicate the acute immune injury model,to observe the effects of YPF-P on spleen recovery. Besides,YPF-P positive control group was established to observe its effects on the spleen structure and function of normal mice. Testing indicators include spleen morphology and tissue structure,body mass,and spleen index. [Results]In the protective group and the recovery group,the spleen volume of the mice with different YPF-P doses increased significantly,and lymphocytes proliferated,showing obvious structural compensation and repair,and showing a dose-effect relationship. In the positive control group,the spleen function of the normal mice was significantly enhanced,and the spleen lymphoid tissue showed significant orderly proliferation. [Conclusions]Yupingfeng polysaccharides can effectively protect the function and structure of spleen,improve acute and chronic immune damage induced by cyclophosphamide,greatly promote the recovery of spleen structure and function after immune injury,and also can significant enhance the spleen structure and function of normal mice.
文摘Objective: To study the effect on apoptosis of CD 4 +T、CD 19 +B in spleen of BXSB mice with systemic lupus erythematosus treated with Langchuangjing Granule (LCJG, and to probe into the mechanism of the tr eatment. Methods: The apoptosis was examined by the flow cytometric analysis and immunofluorescence double-staining method. Results: Apoptosis of male BXSB mice speeds up. LCJG can restrain the excessive apoptosis of CD 4 +T and CD 19 +B cells in spleen. Conclusion: LCJG treated systemic lupus erythematosus by restraining the excessive apoptosis of T, B lymphocytes, probably restraining the release of excessive amount of apoptotic DNA fragments, so decreasing abnormal proliferation of B cells and the produce of autoantibodies.
基金Supported by Natural Science Fund of Guangdong Province(S2013010012191)Science and Technology Project of Guangdong Province(2009B020307004)
文摘[Objective] The paper was to discuss regulation and protection effect of Yupingfeng polysaccharide （YPF-P） on immunosuppressive mice. [Method] Taking immunosuppressive mice induced by 80 mg/kg cyclophosphamide as test animal model, effects of different doses （100,200 and 400 mg/kg） of YPF-P on spleen index, serum IL-2 and IFN-γ levels of mice were studied. [ Result] High dose of YPF-P （400 mg/kg） could improve the spleen index of immunosuppressive mice （P 〈0.05）. YPF-P could enhance serum IL-2 and IFN-γ levels of immunosuppressive mice, and the effects in middle-dose group and low-dose group were better. YPF-P could significantly increase serum IL-2 level of normal mice （ P 〈 0.01 ）, but it had no significant effect on serum IFN-γ level of normal mice. [ Conclusion] The research provides an experimental basis for clinical application of YPF-P.
文摘Mice were watered with cerous nitrate solution (2, 20, 200 mg·kg-1) according to daily drinking water volume for 50 days and the effects of cerous nitrate on nonspecific immunity in mice were investigated. The results showed that the total number of peripheral white blood cell, the clearance rate of charcoal particles, the indices of thymus and spleen were increased on different degrees, and associated with the watering dose, noting that these indices were increased significantly by cerous nitrate at a dose of 20 mg·kg-1 compared with control group. Therefore, it was concluded that the nonspecific immunity could be improved by rare earth Ce in mice.
