Objective To investigate the expression of miRNA-182 in hepatocellular carcinoma(HCC)cell lines and tissues,to verify the targeted regulation relationship between miRNA-182 and forkhead box transcription factor 2(FOXF...Objective To investigate the expression of miRNA-182 in hepatocellular carcinoma(HCC)cell lines and tissues,to verify the targeted regulation relationship between miRNA-182 and forkhead box transcription factor 2(FOXF2)and to explore whether miRNA-182 can promote the invasion,metastasis and angiogenesis of HCC by targeting FOXF2.Methods From January to October 2017,a total of 41 patients with primary HCC admitted to Cancer Hospital Affiliated to Zhengzhou University and receiving operation were enrolled.The expression of miRNA-182 in tumor tissues and normal paracancerous tissues was detected by quantitative real-time fluorescence polymerase chain reaction(qRT-PCR).The effects of overexpression of miRNR-182 and FOXF2 on invasion and metastasis of HCC were detected by Transwell transfer experiment and the Boyden invasion experiment.The effects of miRNA-182 and FOXF2 on angiogenesis of HCC were determinded by in vitro angiogenesis experiments.展开更多
文摘Objective To investigate the expression of miRNA-182 in hepatocellular carcinoma(HCC)cell lines and tissues,to verify the targeted regulation relationship between miRNA-182 and forkhead box transcription factor 2(FOXF2)and to explore whether miRNA-182 can promote the invasion,metastasis and angiogenesis of HCC by targeting FOXF2.Methods From January to October 2017,a total of 41 patients with primary HCC admitted to Cancer Hospital Affiliated to Zhengzhou University and receiving operation were enrolled.The expression of miRNA-182 in tumor tissues and normal paracancerous tissues was detected by quantitative real-time fluorescence polymerase chain reaction(qRT-PCR).The effects of overexpression of miRNR-182 and FOXF2 on invasion and metastasis of HCC were detected by Transwell transfer experiment and the Boyden invasion experiment.The effects of miRNA-182 and FOXF2 on angiogenesis of HCC were determinded by in vitro angiogenesis experiments.