The manifold physical signals including micro resistance,infrared thermal signal and acoustic emission signal in the tensile test for double-material friction welding normative samples were monitored and collected dyn...The manifold physical signals including micro resistance,infrared thermal signal and acoustic emission signal in the tensile test for double-material friction welding normative samples were monitored and collected dynamically by TH2512 micro resistance measuring apparatus,flir infrared thermal camera and acoustic emission equipment which possesses 18 bit PCI-2 data acquisition board.Applied acoustic emission and thermal infrared NDT(non-destructive testing) means were used to verify the feasibility of using resistance method and to monitor dynamic damage of the samples.The research of the dynamic monitoring system was carried out with multi-information fusion including resistance,infrared and acoustic emission.The results show that the resistance signal,infrared signal and acoustic emission signal collected synchronously in the injury process of samples have a good mapping.Electrical,thermal and acoustic signals can more accurately capture initiation and development of micro-defects in the sample.Using dynamic micro-resistance method to monitor damage is possible.The method of multi-information fusion monitoring damage possesses higher reliability,which makes the establishing of health condition diagnosing and early warning platform with multiple physical information monitoring possible.展开更多
Small RNAs play an important role in plant immune responses. However, their regulatory function in induced systemic resistance(ISR) is nascent. Bacillus cereus AR156 is a plant growth-promoting rhizobacterium that i...Small RNAs play an important role in plant immune responses. However, their regulatory function in induced systemic resistance(ISR) is nascent. Bacillus cereus AR156 is a plant growth-promoting rhizobacterium that induces ISR in Arabidopsis against bacterial infection. Here,by comparing small RNA profiles of Pseudomonas syringae pv. tomato(Pst) DC3000-infected Arabidopsis with and without AR156 pretreatment, we identified a group of Arabidopsis micro RNAs(mi RNAs) that are differentially regulated by AR156 pretreatment. mi R825 and mi R825 are two mi RNA generated from a single mi RNA gene.Northern blot analysis indicated that they were significantly downregulated in Pst DC3000-infected plants pretreated with AR156, in contrast to the plants without AR156 pretreatment. mi R825 targets two ubiquitin-protein ligases,while mi R825 targets toll-interleukin-like receptor(TIR)-nucleotide binding site(NBS) and leucine-rich repeat(LRR)type resistance(R) genes. The expression of these target genes negatively correlated with the expression of mi R825 and mi R825. Moreover, transgenic plants showing reduced expression of mi R825 and mi R825 displayed enhanced resistance to Pst DC3000 infection, whereas transgenic plants overexpressing mi R825 and mi R825 were more susceptible. Taken together, our data indicates that Bacillus cereus AR156 pretreatment primes ISR to Pst infection by suppressing mi R825 and mi R825 and activating the defense related genes they targeted.展开更多
基金Project(51125023) supported by Distinguished Young Scholars of Natural Science Foundation of ChinaProject(2011CB013405) supported by the National Basic Research Program of China+1 种基金Project supported by China Equipment Maintenance ProgramProject (3120001) supported by the Natural Science Foundation of Beijing,China
文摘The manifold physical signals including micro resistance,infrared thermal signal and acoustic emission signal in the tensile test for double-material friction welding normative samples were monitored and collected dynamically by TH2512 micro resistance measuring apparatus,flir infrared thermal camera and acoustic emission equipment which possesses 18 bit PCI-2 data acquisition board.Applied acoustic emission and thermal infrared NDT(non-destructive testing) means were used to verify the feasibility of using resistance method and to monitor dynamic damage of the samples.The research of the dynamic monitoring system was carried out with multi-information fusion including resistance,infrared and acoustic emission.The results show that the resistance signal,infrared signal and acoustic emission signal collected synchronously in the injury process of samples have a good mapping.Electrical,thermal and acoustic signals can more accurately capture initiation and development of micro-defects in the sample.Using dynamic micro-resistance method to monitor damage is possible.The method of multi-information fusion monitoring damage possesses higher reliability,which makes the establishing of health condition diagnosing and early warning platform with multiple physical information monitoring possible.
基金supported by a Joint Research Fund for Overseas,Hong Kong and Macao Scholars(31228018)to HJ and JGNIH grant(R01GM093008)to HJ+5 种基金NIH grant-(R01GM100364)a grant from Natural Science Foundation of Jiangsu Province of China(BK20141360)a PhD Programs Foundation of Ministry of Education of China(B0201300664)to HZan National Science Foundation grant(DBI-0743797)to WZa Talent Development Program of Wuhan,the municipal government of Wuhan,Hubei,China(2014070504020241)an internal research grant of Jianghan University,Wuhan,China to WZ
文摘Small RNAs play an important role in plant immune responses. However, their regulatory function in induced systemic resistance(ISR) is nascent. Bacillus cereus AR156 is a plant growth-promoting rhizobacterium that induces ISR in Arabidopsis against bacterial infection. Here,by comparing small RNA profiles of Pseudomonas syringae pv. tomato(Pst) DC3000-infected Arabidopsis with and without AR156 pretreatment, we identified a group of Arabidopsis micro RNAs(mi RNAs) that are differentially regulated by AR156 pretreatment. mi R825 and mi R825 are two mi RNA generated from a single mi RNA gene.Northern blot analysis indicated that they were significantly downregulated in Pst DC3000-infected plants pretreated with AR156, in contrast to the plants without AR156 pretreatment. mi R825 targets two ubiquitin-protein ligases,while mi R825 targets toll-interleukin-like receptor(TIR)-nucleotide binding site(NBS) and leucine-rich repeat(LRR)type resistance(R) genes. The expression of these target genes negatively correlated with the expression of mi R825 and mi R825. Moreover, transgenic plants showing reduced expression of mi R825 and mi R825 displayed enhanced resistance to Pst DC3000 infection, whereas transgenic plants overexpressing mi R825 and mi R825 were more susceptible. Taken together, our data indicates that Bacillus cereus AR156 pretreatment primes ISR to Pst infection by suppressing mi R825 and mi R825 and activating the defense related genes they targeted.