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Loss of micro RNA-124 expression in neurons in the peri-lesion area in mice with spinal cord injury 被引量:7
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作者 Yu Zhao Hui Zhang +6 位作者 Dan Zhang Cai-yong Yu Xiang-hui Zhao Fang-fang Liu Gan-lan Bian Gong Ju Jian Wang 《Neural Regeneration Research》 SCIE CAS CSCD 2015年第7期1147-1152,共6页
Micro RNA-124(mi R-124) is abundantly expressed in neurons in the mammalian central nervous system, and plays critical roles in the regulation of gene expression during embryonic neurogenesis and postnatal neural di... Micro RNA-124(mi R-124) is abundantly expressed in neurons in the mammalian central nervous system, and plays critical roles in the regulation of gene expression during embryonic neurogenesis and postnatal neural differentiation. However, the expression profile of mi R-124 after spinal cord injury and the underlying regulatory mechanisms are not well understood. In the present study, we examined the expression of mi R-124 in mouse brain and spinal cord after spinal cord injury using in situ hybridization. Furthermore, the expression of mi R-124 was examined with quantitative RT-PCR at 1, 3 and 7 days after spinal cord injury. The mi R-124 expression in neurons at the site of injury was evaluated by in situ hybridization combined with Neu N immunohistochemical staining. The mi R-124 was mainly expressed in neurons throughout the brain and spinal cord. The expression of mi R-124 in neurons significantly decreased within 7 days after spinal cord injury. Some of the neurons in the peri-lesion area were Neu N+/mi R-124-. Moreover, the neurons distal to the peri-lesion site were Neu N+/mi R-124+. These findings indicate that mi R-124 expression in neurons is reduced after spinal cord injury, and may reflect the severity of spinal cord injury. 展开更多
关键词 nerve regeneration spinal cord injury micro RNA spinal cord in situ hybridization immunohistochemistry digoxin Neu N protein brain neural plasticity repair apoptosis NSFC grants neural regeneration
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Altered microRNA expression profiles in a rat model of spina bifida 被引量:1
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作者 Pan Qin Lin Li +5 位作者 Da Zhang Qiu-liang Liu Xin-rang Chen He-ying Yang Ying-zhong Fan Jia-xiang Wang 《Neural Regeneration Research》 SCIE CAS CSCD 2016年第3期502-507,共6页
Micro RNAs(mi RNAs) are dynamically regulated during neurodevelopment,yet few reports have examined their role in spina bifida.In this study,we used an established fetal rat model of spina bifida induced by intragas... Micro RNAs(mi RNAs) are dynamically regulated during neurodevelopment,yet few reports have examined their role in spina bifida.In this study,we used an established fetal rat model of spina bifida induced by intragastrically administering olive oil-containing all-trans retinoic acid to dams on day 10 of pregnancy.Dams that received intragastric administration of all-trans retinoic acid-free olive oil served as controls.The mi RNA expression profile in the amniotic fluid of rats at 20 days of pregnancy was analyzed using an mi RNA microarray assay.Compared with that in control fetuses,the expression of mi RNA-9,mi RNA-124 a,and mi RNA-138 was significantly decreased(〉 2-fold),whereas the expression of mi RNA-134 was significantly increased(〉 4-fold) in the amniotic fluid of rats with fetuses modeling spina bifida.These results were validated using real-time quantitative reverse-transcription polymerase chain reaction.Hierarchical clustering analysis of the microarray data showed that these differentially expressed mi RNAs could distinguish fetuses modeling spina bifida from control fetuses.Our bioinformatics analysis suggested that these differentially expressed mi RNAs were associated with many cytological pathways,including a nervous system development signaling pathway.These findings indicate that further studies are warranted examining the role of mi RNAs through their regulation of a variety of cell functional pathways in the pathogenesis of spina bifida.Such studies may provide novel targets for the early diagnosis and treatment of spina bifida. 展开更多
关键词 nerve regeneration spina bifida amniotic fluid all-trans retinoic acid microarray micro RNA reverse transcription-polymerase chain reaction MAPK neural regeneration
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