目的:研究结肠癌组织中微小RNA-183-5p(miR-183-5p)和硫酯酶超家族成员4(THEM4)的表达水平及其临床意义。方法:收集河北中石油中心医院96例结肠癌患者为研究对象,在进行根治切除术过程中收集患者结肠癌组织及癌旁正常组织。检测结肠癌...目的:研究结肠癌组织中微小RNA-183-5p(miR-183-5p)和硫酯酶超家族成员4(THEM4)的表达水平及其临床意义。方法:收集河北中石油中心医院96例结肠癌患者为研究对象,在进行根治切除术过程中收集患者结肠癌组织及癌旁正常组织。检测结肠癌组织及癌旁正常组织中miR-183-5p和THEM4 m RNA的相对表达水平。分析两者的相关性及其与预后的关系。Cox回归分析影响结肠癌患者预后的危险因素。结果:与癌旁正常组织相比,结肠癌组织中miR-183-5p表达水平升高(P<0.05),THEM4 m RNA表达水平降低(P<0.05)。结肠癌组织中miR-183-5p与THEM4 m RNA表达呈负相关(r=-0.529,P<0.05)。miR-183-5p高表达组生存率低于低表达组(P<0.05),THEM4高表达组生存率显著高于低表达组(P<0.05)。TNM分期(Ⅲ~Ⅳ)、miR-183-5p高表达、THEM4低表达是结肠癌患者不良预后的危险因素(P<0.05)。结论:结肠癌织中miR-183-5p表达水平升高,THEM4表达水平降低,两者均与患者临床病理特征及预后密切相关。展开更多
Many studies have illustrated the significance of long noncoding RNAs in oncogenesis and promotion of breast cancer(BC).However,the biological roles of CCDC183 antisense RNA 1(CCDC183-AS1)in BC have rarely been charac...Many studies have illustrated the significance of long noncoding RNAs in oncogenesis and promotion of breast cancer(BC).However,the biological roles of CCDC183 antisense RNA 1(CCDC183-AS1)in BC have rarely been characterized.Thus,we explored whether CCDC183-AS1 is involved in the malignancy of BC and elucidated the possible underlying mechanisms.Our data confirmed elevated CCDC183-AS1 expression in BC,which was associated with poor clinical outcomes.Functionally,knocking down CCDC183-AS1 hampered cell proliferation,colony formation,migration,and invasion in BC.Additionally,the absence of CCDC183-AS1 restrained tumor growth in vivo.Mechanistically,CCDC183-AS1 executed as a competitive endogenous RNA in BC cells by decoying microRNA-3918(miR-3918)and consequently overexpressing fibroblast growth factor receptor 1(FGFR1).Furthermore,functional rescue experiments confirmed that inactivation of the miR-3918/FGFR1 regulatory axis by inhibiting miR-3918 or increasing FGFR1 expression could abrogate the CCDC183-AS1 ablation-mediated repressive effects in BC cells.In summary,CCDC183-AS1 deteriorates the malignancy of BC cells by controlling miR-3918/FGFR1 regulatory axis.We believe that our study can deepen our understanding of BC etiology and contribute to an improvement in treatment choices.展开更多
文摘目的:研究结肠癌组织中微小RNA-183-5p(miR-183-5p)和硫酯酶超家族成员4(THEM4)的表达水平及其临床意义。方法:收集河北中石油中心医院96例结肠癌患者为研究对象,在进行根治切除术过程中收集患者结肠癌组织及癌旁正常组织。检测结肠癌组织及癌旁正常组织中miR-183-5p和THEM4 m RNA的相对表达水平。分析两者的相关性及其与预后的关系。Cox回归分析影响结肠癌患者预后的危险因素。结果:与癌旁正常组织相比,结肠癌组织中miR-183-5p表达水平升高(P<0.05),THEM4 m RNA表达水平降低(P<0.05)。结肠癌组织中miR-183-5p与THEM4 m RNA表达呈负相关(r=-0.529,P<0.05)。miR-183-5p高表达组生存率低于低表达组(P<0.05),THEM4高表达组生存率显著高于低表达组(P<0.05)。TNM分期(Ⅲ~Ⅳ)、miR-183-5p高表达、THEM4低表达是结肠癌患者不良预后的危险因素(P<0.05)。结论:结肠癌织中miR-183-5p表达水平升高,THEM4表达水平降低,两者均与患者临床病理特征及预后密切相关。
文摘Many studies have illustrated the significance of long noncoding RNAs in oncogenesis and promotion of breast cancer(BC).However,the biological roles of CCDC183 antisense RNA 1(CCDC183-AS1)in BC have rarely been characterized.Thus,we explored whether CCDC183-AS1 is involved in the malignancy of BC and elucidated the possible underlying mechanisms.Our data confirmed elevated CCDC183-AS1 expression in BC,which was associated with poor clinical outcomes.Functionally,knocking down CCDC183-AS1 hampered cell proliferation,colony formation,migration,and invasion in BC.Additionally,the absence of CCDC183-AS1 restrained tumor growth in vivo.Mechanistically,CCDC183-AS1 executed as a competitive endogenous RNA in BC cells by decoying microRNA-3918(miR-3918)and consequently overexpressing fibroblast growth factor receptor 1(FGFR1).Furthermore,functional rescue experiments confirmed that inactivation of the miR-3918/FGFR1 regulatory axis by inhibiting miR-3918 or increasing FGFR1 expression could abrogate the CCDC183-AS1 ablation-mediated repressive effects in BC cells.In summary,CCDC183-AS1 deteriorates the malignancy of BC cells by controlling miR-3918/FGFR1 regulatory axis.We believe that our study can deepen our understanding of BC etiology and contribute to an improvement in treatment choices.
