AIM: To investigate microRNA-133a(miR-133a) expression in colorectal cancer(CRC) and its relationship with tumorigenesis and disease prognosis.METHODS: Quantitative real-time polymerase chain reaction was used to meas...AIM: To investigate microRNA-133a(miR-133a) expression in colorectal cancer(CRC) and its relationship with tumorigenesis and disease prognosis.METHODS: Quantitative real-time polymerase chain reaction was used to measure levels of miR-133 a in tumor samples and adjacent non-cancerous tissues from 169 patients undergoing radical resection for CRC. The associations between miR-133 a expression and patient age, sex, as well as clinicopathologic parameters, such as tumor size, differentiation, location, invasion depth, metastasis, tumor-node-metastasis(TNM) stage and overall patient survival, were analyzed by MannWhitney U and Kruskal-Wallis tests. The Kaplan-Meier method and Cox proportional hazards regression analyses were performed to estimate the prognostic factors for patient survival prediction.RESULTS: The expression of miR-133 a was significantly downregulated in CRC tissues compared with adjacent non-cancerous tissues(P < 0.05). This reduction was associated with the depth of the local invasion, poor differentiation, lymph node metastasis and advanced disease(P < 0.05). Moreover, Kaplan-Meier analysis demonstrated that patients with low miR-133 a expression had poorer overall survival(OS) than those with high miR-133 a expression(P < 0.001). Univariate analysis revealed statistically significant correlations between OS and miR-133 a level, tumor local invasion, lymph node metastasis and TNM stage(P < 0.001). Furthermore, miR-133 a levels and TNM stage were independently associated with OS(HR = 0.590, 95%CI: 0.350-0.995, P < 0.05; and HR = 6.111, 95%CI: 1.029-36.278, P < 0.05, respectively).CONCLUSION: The downregulation of miR-133 a may play an important role in the progression of CRC and can be used as an independent factor to determine CRC prognosis.展开更多
基金Supported by Grants from the National Key Clinical Medical Specialties Foundation and the National Natural Science Foundation of China,No.81271916 and No.81301506
文摘AIM: To investigate microRNA-133a(miR-133a) expression in colorectal cancer(CRC) and its relationship with tumorigenesis and disease prognosis.METHODS: Quantitative real-time polymerase chain reaction was used to measure levels of miR-133 a in tumor samples and adjacent non-cancerous tissues from 169 patients undergoing radical resection for CRC. The associations between miR-133 a expression and patient age, sex, as well as clinicopathologic parameters, such as tumor size, differentiation, location, invasion depth, metastasis, tumor-node-metastasis(TNM) stage and overall patient survival, were analyzed by MannWhitney U and Kruskal-Wallis tests. The Kaplan-Meier method and Cox proportional hazards regression analyses were performed to estimate the prognostic factors for patient survival prediction.RESULTS: The expression of miR-133 a was significantly downregulated in CRC tissues compared with adjacent non-cancerous tissues(P < 0.05). This reduction was associated with the depth of the local invasion, poor differentiation, lymph node metastasis and advanced disease(P < 0.05). Moreover, Kaplan-Meier analysis demonstrated that patients with low miR-133 a expression had poorer overall survival(OS) than those with high miR-133 a expression(P < 0.001). Univariate analysis revealed statistically significant correlations between OS and miR-133 a level, tumor local invasion, lymph node metastasis and TNM stage(P < 0.001). Furthermore, miR-133 a levels and TNM stage were independently associated with OS(HR = 0.590, 95%CI: 0.350-0.995, P < 0.05; and HR = 6.111, 95%CI: 1.029-36.278, P < 0.05, respectively).CONCLUSION: The downregulation of miR-133 a may play an important role in the progression of CRC and can be used as an independent factor to determine CRC prognosis.
