Effects of Storage Temperature on Postharvest Physiology of Amorphophallus cormifer Microbulb

[Objectives]The paper was to explore the optimum storage temperature of Amorphophallus cormifer microbulbs.[Methods]With A.cormifer as the raw material,the effects of different storage temperatures(4,12 and 20℃)on postharvest physiology of A.cormifer during the storage period of 80 d were investigated.[Results]There was no significant difference in starch content among the treatments,and the content of reducing sugar at 4℃was significantly higher than those of other treatments during the storage period.There was no significant difference in total water content among treatments,and the specific gravity of free water at 4℃was significantly lower than those at 12 and 20℃,respectively.At 60 d post storage,the POD activity at 4℃was significantly higher than those at 12 and 20℃,respectively.At the 80th day of storage,the PPO activity at 4℃was significantly lower than those at 12 and 20℃,respectively.[Conclusions]The low temperature of 4℃is more conducive to the storage of A.cormifer microbulbs,and the results also provide the theoretical basis for long-term storage of A.cormifer bulbs.

嘉定白蒜脱毒微鳞茎诱导的快繁技术研究

以嘉定白蒜为试材,研究了不同茎尖脱毒方法与不同外植体启动培养基对嘉定白蒜无菌苗建立与脱毒率的影响、不同增殖培养基与切割方式对潜伏芽激活与增殖的影响,以及组培苗分株等级标准与培养基配方对诱导组培微鳞茎形成的影响。结果显示:带1个叶原基的茎尖,在MS+6-BA1.0mg·L^(-1)+NAA0.1mg·L^(-1)的培养基上成苗率高,达76%;采用单株假茎去顶后,取其基部1.0cm假茎茎段,再沿中轴线对半纵切的方式,在MS+6-BA1.0mg·L^(-1)+NAA0.10mg·L^(-1)的培养基上能较好地激活叶腋潜伏芽,增殖倍数达3.22;1个叶原基包裹的芽原基茎尖脱毒成效最好,达100%;假茎茎粗3mm的组培苗单株,在MS+NAA0.1mg·L^(-1)+糖90g·L^(-1)、pH为7.0的培养基中微鳞茎诱导率最高,达94.8%。茎尖脱毒方式、叶腋潜伏芽激活切割方式和组培微鳞茎诱导的培养基配方,是嘉定白蒜脱毒与快繁的技术关键。