Recent progress in microbial cell surface display systems and their application on biosensors

Microbial cell surface display technology is a recombinant technology to express target proteins on the cell membrane,which can be used to redesign the cell surface with functional proteins and peptides.Bacterial and yeast surface display systems are the most common cell surface display systems of prokaryotic and eukaryotic proteins,that are widely applied as the core elements in the field of biosensors due to their advantages,including enhanced stability,high yield,good safety,expression of larger and more complex proteins.To further promote the performance of biosensors,the biomineralized microbial surface display technology was proposed.This review summarized the different microbial surface display systems and the biomineralized surface display systems,where the mechanisms of surface display and biomineralization were introduced.Then we described the recent progress of their applications on biosensors for different types of detection targets.Finally,the outlooks and tendencies were discussed and forecasted with the expectation to provide some general functions and enlightenments to this aspect of research.

NOVEL VOLUMETRIC THREE-DIMENSIONAL DISPLAY SYSTEM

A novel volumetric three-dimensional（3-D） display system is developed based on the human eye persistence and the system fuses a time-series of image slices into a single hologram like 3-D aerial image. The system design is introduced and key components are described. Experimental results show that the 3-D system can guide people freely walk around the display to inspect the true 3-D image without goggles.

An Experimental Study on Microcomputer Control of Pump Controlled Motor Systems

Pump controlled motor electrohydraulic servo systems are much used in circumstances where high power drive is needed. This kind of system has the advantage of energy-saving. But, it also has some defects that have to be improved. Microcomputer control of a pump controlled motor electrohydraulic servo system is studied. A PID controller is first adopted on the closed loop control system, and experimental results are obtained. Then, a model reference adaptive controller is designed and realised on the same system applying a single board microcomputer. Experimental results show that the dynamic properties of the adaptive control system is much better than those of the PID system under different inertia load conditions.

Design of Intelligent Fire Alarm System for Large Storage Places Based on AVRmega128 Single Chip Microcomputer

With principles of reliability, independence, practicality and economical effi- ciency, a set of intelligent fire alarm system based on AVRmega128 single chip microcomputer was designed to solve problems of fire alarm system in many large- scale warehouses. Using advanced flame sensor, 485 bus communication, computer interactive software and related peripheral devices, this intelligent fire alarm system has functions of sound-light alarm and intelligent fire extinguishing. The human-com- puter interactive software was adopted for the remote control of the alarm main control panel through the 485 bus communication. This design of intelligent fire alarm system shows high reference and practical value to the development of intel- ligent alarm products with high integration and high reliability.

Use of single chip microcomputer in hydraulic digital adaptive control system

Presents a one grade adaptive controller with one reference model which is built according to δ MRACS adaptive control theory and used to control an actual high order hydraulic system, and the whole hardware system used, which includes a AT89C51 single chip microcomputer, 74Ls373 flip latch, 6116 store, eight bit ADC0809, and so on, and the satisfactory results obtained in study on hydraulic control system.

VOLUMETRIC-SWEPT DISPLAY SYSTEM BASED ON HELIX ROTATING SCREEN AND DMD

Based on the helix rotating screen and the digital micro-mirror device （DMD）, the former proto of volumetric-swept display system is improved. The 3-D display system adopting a helix rotating screen to construct an imaging space meliorate the defects, such as the smaller image space, the fewer voxels and the severer voxel overlap dead zone caused by planar rotating screen. DMD with spatial light modular （SLM） technology increases the transmission bandwidth of 3-D data in the voxel activation subsystem and activate multi-voxel once time. The volumetric-swept system based on helix rotating screen and DMD is developed. The experimental results show that the image space, the vision dead zone, the voxels on slice, and the voxel activation capacity of the designed proto are superior to the plane rotating screen system.

Research on A Master - slave Multi - microcomputers Control System for Hollow Spindle Fancy Yarn Spinning Machine

In this paper, a successfully studied and developed master - slave muld - microcomputers control system based on PC - BUS for hollow spindle fancy yarn spinning machine, mainly Its overall scheme, software and hardware construction, is introduced. Spinning experiments show that the system achieves satisfactory result. This system can solve the diftkultles of mechatronical fusion between domestic hollow splndk fancy yarn spuming muchine and its microcomputer control technology.

Study of Traction Motor Feedback Testing SystemControlled by Microcomputer

this paper describes the design of a feedback testing system for locomtive tractionmotor. The structures and features of the software and hardware of themicrocomputer control system have been studied. This testing system hasalready been put into operation successfully in several locomotive depots inChina.

Model Implementation and Analysis of a True Three-dimensional Display System

To model a true three-dimensional(3D)display system,we introduced the method of voxel molding to obtain the stereoscopic imaging space of the system.For the distribution of each voxel,we proposed a four-dimensional(4D)Givone–Roessor(GR)model for state-space representation—that is,we established a local state-space model with the 3D position and one-dimensional time coordi-nates to describe the system.First,we extended the original elementary operation approach to a 4D condition and proposed the implementation steps of the realiza-tion matrix of the 4D GR model.Then,we described the working process of a true 3D display system,analyzed its real-time performance,introduced the fixed-point quantization model to simplify the system matrix,and derived the conditions for the global asymptotic stability of the system after quantization.Finally,we provided an example to prove the true 3D display system’s feasibility by simulation.The GR-model-representation method and its implementation steps proposed in this paper simplified the system’s mathematical expression and facilitated the microcon-troller software implementation.Real-time and stability analyses can be used widely to analyze and design true 3D display systems.

Construction of a Multipurpose M13KE Phage Display System

In this study, a multipurpose M13KE phage display vector was constructed from wild-type M13KE phage for long peptide or protein display libraries without helper phage to expand the scope of targeted high-throughput screening. Based on the relationship between the structure and function of minor coat protein of wild-type MI3KE （wt-plII）, a truncated gene III （tglll） encoding minor coat protein from M13KE phage was cloned. A fusion gene fragment harboring a hw/tac promoter, signal peptide and C-terminal region sequence of gill was assembled with SOEing-PCR （splice-overlapping-extension polymerase chain reaction） method and inserted into M13KE vector. SDS-PAGE and Western blot analysis with anti-M13 pIII moneclonal antibody were employed to detect the expression of re- combinant protein, c-Myc and HA tag sequences were fused into the recombinant protein. The results showed that tglll was inserted into an unessential region of M13KE. According to the results of SDS-PAGE and Western blot with anti-M13 pIII antibody, pIII was expressed by wt-gIII and tgIII, glII harboring two tags ex- pressed both c-Myc and HA peptides using SDS-PAGE and Western blot with the corresponding monoclonal antibodies. In this study, a multipurpose M13KE phage display system was successfully constructed, which could express both short and long peptide libraries without helper phage. In future, the obtained M13KE phage display system may be used for targeted high-throughput screening of long peptide libraries without helper phage.

MICROCOMPUTER PICTURE PROCESSING SYSTEM OF CAST-IRON METALLOGRAPHIC STRUCTURE

The development of computer picture processing technique in metallography analysis is dealed with and a picture processing procedure fit to metallography analysis is developed. The image processing of nodular cast iron is carried out with this system

A MICROCOMPUTER ANALYTIC SYSTEM FOR CRYSTAL STRUCTURE USED IN CHEMICAL LABORATORIES

The program system described in this paper is a microcomputer analytic system for X-ray crystal structure used in chemical laboratories.The abbreviated name is NOMCSDP.NOMCSDP has been developed on the basis of NRCVAX and SHELXS-86.It is a software for X- ray crystal structure analysis of organic molecules,especially natural organic molecules(1-7).

Development of an Instant Correction and Display System of Numerical Weather Prediction Products in China

This paper presents the development of numerical prediction products(NPP) correction and display system(NPPCDS) for rapid and effective post-processing and displaying of the T213 NPP(numerical prediction products of the medium range numerical weather prediction spectral model T213L31) through instant correction method. The NPPCDS consists of two modules: an automatic correction module and a graphical display module. The automatic correction module automatically corrects the T213 NPP at regularly scheduled time intervals, while the graphical display module interacts with users to display the T213 NPP and its correction results. The system helps forecasters extract the most relevant information at a quick glance without extensive post-processing. It is simple, easy to use, and computationally efficient, and has been running stably at Huludao Meteorological Bureau in Liaoning Province of China for the past three years. Because of its low computational costs, it is particularly useful for meteorological departments that lack advanced computing capacity and still need to make short-range weather forecasting.

Phage Display Technology, Phage Display System, Antibody Library, Prospects and Challenges

Phage display technology is a unique gene recombination expression technology, and it is also a simple and effective screening tool. Through panning, a protein or peptide with high affinity and selectivity to the target is obtained. Antibody phage display has become the first and most widely used in vitro screening technology. Phage display derivatives play an important role in the diagnosis and treatment of diseases. This article reviews the phage display system of phage display technology, the size and classification of antibody libraries and their applications, and discusses the application prospects and challenges of phage display technology. This thesis lays the foundation for the theoretical and experimental research of bacteriophages.

Design of an Automated Sorting System for Apples Based on Single Chip Microcomputer

The grading judgment for apples is related to a variety of factors including,size,shape,color,texture,and scars.Traditional manual sorting methods are time consuming and labor intensive.In addition,the accuracy of the method is easily subjective,not repeatable,error-prone,and affected by the sorting environment.This paper presents a complete and automated grading system for apples.The system uses a single-chip microcomputer as the controller of the system,and a PC as the graphics processing unit.It also includes a conveyor,drive motor,frequency converter for motor control,photoelectric sensors,air compressor,and air jets for ejecting the graded apples.The classification algorithm is implemented by using a convolutional neural network(CNN).In order to eliminate contact damage of apples,the system specifically uses air jets as actuators to eject the graded apples into the corresponding bins.At the same time,in order to ensure that an apple triggers the correct ejecting actuator,this paper designs a jet controller with proper logic.

Low-Cost Tiled Display System Architecture with Dynamic Web-Based Management

In this paper, a scalable hardware and software architecture for tiled display systems (a.k.a. videowalls), which can be implemented by using low-cost devices, together with a dynamic web-based management and configuration service are proposed. It has been designed to support both stored and live broadcast/broadband content, in mosaic or warp distributions. The displays and devices can be dynamically configured via web in different ways: the displays can create a single display of a larger size;or they can be configured in a customized way in order to playout different media contents in different display combinations. As display renderers, low-cost devices are proposed as the main hardware element to obtain affordable videowall systems. As a proof of concept, two prototypes have been implemented, including an accurate synchronization mechanism based on a Master/Slave control scheme and aggressive and smooth playout adjustment techniques. To evidence the good performance of the prototypes and configuration service, both objective and subjective evaluations have been conducted regarding synchronization accuracy and usability. On the one hand, the mean values of the asynchronies between the video playout processes in each display are kept below 25ms (i.e., frame accuracy). On the other hand, the obtained usability score in the System Usability Scale (SUS) test has been 88.65, which is considered as excellent.

3D Display System Based On Digital Holography Application In Making Cartoon Holography

Holography is an interesting tool in creating real objects and scenes which can be projected anywhere with accurate details and depth impression. It is also found to be more attractive to the artists than other alternatives. For that reason, digital holography is being used as a display technology in cartoon movies. Since this application is dependent on the performance and the simplicity of the available display technology, it becomes very useful to improve the display technique in order to become fast, simple, and attractive by being combined with computer graphical effects. This paper discusses a simulation of a digital holographic model as a three dimensional (3D) display system and its application in making cartoon holography.

Swept-Volume Display System Based on Cylindrical Space Projection and Curved Reflectors

In order to achieve a clear and steady swept-volume display,the method of swept-volume display based on cylindrical space projection was presented. One projector generated the image volume in π× 70 mm × 70 mm × 150 mm space. Experimentally,the resolution of images was 800 pixel × 600 pixel × 360 pixel,which resulted in almost 345 million voxels. In order to achieve space voxels with uniform brightness, curved reflectors were also designed. In addition,the match conditions between triangles and the scanning planes in the volume space were classified and a sweptvolume graphics engine based on embedded platform was designed.The image rendering the hardware foundation for three-dimensional( 3D) dynamic images generation was achieved. Demonstrated in the experiments,light source utilization of the second-generation system based on curved mirror is about three times brighter than the firstgeneration 3D minitor based on flat mirror,and this system is able to display color,clear and well-proportioned 3D images in brighter room light.

Airport Management—Value of Customer Display Systems

By providing real-time updates of essential information, airports not only display and disseminate information but also help control the flow of traffic. In order to maximize available space, particularly in high traffic areas, Airport Display Information Systems should be integrated into the overall design of the airport and their positioning should be carefully planned to deliver optimal results. Airport Display Information Systems can help airports maximize space, increase customer satisfaction, and generate new revenue opportunities. The technology is designed not only to comply with environmental regulations, but also to help airports keep budgets in check. This paper discusses airport display systems, their connections and interoperability with other systems and who the key airport users of these airport display systems are.

Design of DC regulated power supply based on single chip microcomputer

A DC regulated power supply with numerical control based on single chip microcomputer （SCM） is designed. SCM is the main controller and output voltage o{ DC power supply can be set by keyboard. The analog voltage can be obtained through D/A converter （DAC0832） so that different voltages can be provided by operational amplifier. The output voltage varies from 0 V to 12 V with the incremental value of 0. 1 V. The actual output voltage is shown in the nixietube. This DC regulated power supply is characterized by simple structure and easy operation.