Access to embryonic developmental stages is essential basic work for understanding how organisms develop. In this study, seven egg clutches（range 209–564 eggs） of ornamented pygmy frog Microhyla fissipes（Amphibia,...Access to embryonic developmental stages is essential basic work for understanding how organisms develop. In this study, seven egg clutches（range 209–564 eggs） of ornamented pygmy frog Microhyla fissipes（Amphibia, Anura, Microhylidae） were obtained from seven breeding pairs in laboratory. One egg clutch of them was observed for the embryonic development, and the staging table of normal development was constructed based on morphological and physiological characteristics. Forty-five developmental stages were defined for M. fissipes, and two major developmental periods were designated： 1） early embryonic development period（stages 1–28）, from fertilization to operculum completion stage, lasted for 82.6 hours at water temperature（WT） 23–25℃; 2） larval development period（stages 29–45）, from operculum completion to tail complete absorption stage, took 38 days at WT 22–26.5℃, showing that the embryos of this species develop rapidly. In addition, the tadpoles were transparent, which is similar to those in field. These characteristics suggest that M. fissipes would be a good model to study developmental biology, adaptive mechanisms from aquatic to terrestrial phases, environmental toxicology, and human disease.展开更多
基金supported by Important Research Project of Chinese Academy of Sciences(KJZG-EW-L13)National Natural Science Foundation of China(NSFC No.31471964)+2 种基金2015 Western Light Talent Culture Project of the Chinese Academy of Sciences(Y6C3021)the Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization of CIB,CASthe Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province
文摘Access to embryonic developmental stages is essential basic work for understanding how organisms develop. In this study, seven egg clutches（range 209–564 eggs） of ornamented pygmy frog Microhyla fissipes（Amphibia, Anura, Microhylidae） were obtained from seven breeding pairs in laboratory. One egg clutch of them was observed for the embryonic development, and the staging table of normal development was constructed based on morphological and physiological characteristics. Forty-five developmental stages were defined for M. fissipes, and two major developmental periods were designated： 1） early embryonic development period（stages 1–28）, from fertilization to operculum completion stage, lasted for 82.6 hours at water temperature（WT） 23–25℃; 2） larval development period（stages 29–45）, from operculum completion to tail complete absorption stage, took 38 days at WT 22–26.5℃, showing that the embryos of this species develop rapidly. In addition, the tadpoles were transparent, which is similar to those in field. These characteristics suggest that M. fissipes would be a good model to study developmental biology, adaptive mechanisms from aquatic to terrestrial phases, environmental toxicology, and human disease.
