Objective:To evaluate the detection of IgM and IgG antibodies to Orientia tsutsugamushi(O. tsutsugamushi) by rapid diagnostic test(RDT) and microimmunofluorescence assay(ml FA). Methods:RDT using a mixture of recombin...Objective:To evaluate the detection of IgM and IgG antibodies to Orientia tsutsugamushi(O. tsutsugamushi) by rapid diagnostic test(RDT) and microimmunofluorescence assay(ml FA). Methods:RDT using a mixture of recombinant 56-kDa proteins of O.tsutsugamushi and mIFA assay were performed on 20 patients from Fujian and 13 patients from Yunnan Province,and 82 sera samples from healthy farmers in Anhui Province and Beijing City in 2009.Comparison of the RDT and mIFA assay was performed by using X test and the P level of 【0.05 was considered to be significance.Results:Among these 82 normal sera samples,the specificity of RDT was 100%for both IgM and IgG tests.In 33 samples from patients with scrub typhus,5 cases were positively detected earlier by RDT than by mIFA in IgM test,and 2 cases were positive in IgG test.Sensitivities of RDT were 93.9%and 90.9%for IgM and IgG,respectively.The sensitivity of combination lest of IgM and IgG was 100%.Geometric mean titer diluted sera from confirmed cases by IFA and RDT assay were 1:37 vs.1:113(P【0.001) in IgM test and 1:99 vs.1:279 (P【0.05) in IgG test.Conclusions:RDT is more sensitivite than mIFA in the early diagnosis of scrub typhus and it is particularly applicable in rural areas.展开更多
Objectives To study the as-sociation between infection with chlamydia pneumoniaand acute myocardial infarction (AMI). MethodsSerology of chlamydia pneumoniae specific IgG、IgMantibodies were measured by microimmunoflu...Objectives To study the as-sociation between infection with chlamydia pneumoniaand acute myocardial infarction (AMI). MethodsSerology of chlamydia pneumoniae specific IgG、IgMantibodies were measured by microimmunofluores-cence test in groups of acute myocardial infarction(AMI) and health control(HC). Results The total in-fection positive rates were 30.6% in HC group and88.1% in AMI group, including the previous infectionrates which were 30.6% and 71.4%, while the acuteinfection rates were 0% and 16.7%. The frequency oftotal infection, previous infection and acute infectionwas significantly higher in AMI group than in the HCgroup. Odds Ratio for the development of AMI were16.82, 5.68, 14.2, respectively(95% CI 5.83 to 48.54,2.46 to 13.11, 1.68 to 119.97). Geometric mean IgGtitre was significantly higher in patients with AMIcompared with the HC group (P<0.01). There is noIgM positive in HC group but there were two cases inAMI group. Conclusions The presence of high titersof immmunoglobulin G in AMI. Chlamydia pneumoniainfection may be a risk factor for the AMI.展开更多
基金supported by the National Basil Research Program of China(973 Program) 2010CBS30200(2010CB530206)the grants from the National Key Science and Technolog Projects of China,No.2009ZX10004-203 and 2008ZX10004-008)
文摘Objective:To evaluate the detection of IgM and IgG antibodies to Orientia tsutsugamushi(O. tsutsugamushi) by rapid diagnostic test(RDT) and microimmunofluorescence assay(ml FA). Methods:RDT using a mixture of recombinant 56-kDa proteins of O.tsutsugamushi and mIFA assay were performed on 20 patients from Fujian and 13 patients from Yunnan Province,and 82 sera samples from healthy farmers in Anhui Province and Beijing City in 2009.Comparison of the RDT and mIFA assay was performed by using X test and the P level of 【0.05 was considered to be significance.Results:Among these 82 normal sera samples,the specificity of RDT was 100%for both IgM and IgG tests.In 33 samples from patients with scrub typhus,5 cases were positively detected earlier by RDT than by mIFA in IgM test,and 2 cases were positive in IgG test.Sensitivities of RDT were 93.9%and 90.9%for IgM and IgG,respectively.The sensitivity of combination lest of IgM and IgG was 100%.Geometric mean titer diluted sera from confirmed cases by IFA and RDT assay were 1:37 vs.1:113(P【0.001) in IgM test and 1:99 vs.1:279 (P【0.05) in IgG test.Conclusions:RDT is more sensitivite than mIFA in the early diagnosis of scrub typhus and it is particularly applicable in rural areas.
文摘Objectives To study the as-sociation between infection with chlamydia pneumoniaand acute myocardial infarction (AMI). MethodsSerology of chlamydia pneumoniae specific IgG、IgMantibodies were measured by microimmunofluores-cence test in groups of acute myocardial infarction(AMI) and health control(HC). Results The total in-fection positive rates were 30.6% in HC group and88.1% in AMI group, including the previous infectionrates which were 30.6% and 71.4%, while the acuteinfection rates were 0% and 16.7%. The frequency oftotal infection, previous infection and acute infectionwas significantly higher in AMI group than in the HCgroup. Odds Ratio for the development of AMI were16.82, 5.68, 14.2, respectively(95% CI 5.83 to 48.54,2.46 to 13.11, 1.68 to 119.97). Geometric mean IgGtitre was significantly higher in patients with AMIcompared with the HC group (P<0.01). There is noIgM positive in HC group but there were two cases inAMI group. Conclusions The presence of high titersof immmunoglobulin G in AMI. Chlamydia pneumoniainfection may be a risk factor for the AMI.
