Degradation of three kinds of bioplastics and their effects on microbial biomass and microbial diversity in soil environment were analyzed. The degradation rate of bioplastic in soil was closely related to the main co...Degradation of three kinds of bioplastics and their effects on microbial biomass and microbial diversity in soil environment were analyzed. The degradation rate of bioplastic in soil was closely related to the main components in the bioplastics. Poly (butylene succinate)-starch (PBS-starch) and poly (butylene succinate) (PBS) were degraded by 1% to 7% after 28 days in a soil with an initial bacterial biomass of 1.4 × 10<sup>9</sup> cells/g-soil, however poly lactic acid (PLA) was not degraded in the soil after 28 days. When the powdered-bioplastics were examined for the degradation in the soil, PBS-starch also showed the highest degradability (24.4% degradation after 28 days), and the similar results were obtained in the case of long-term degradation experiment (2 years). To investigate the effect of bacterial biomass in soil on biodegradability of bioplastics, PBS-starch was buried in three kinds of soils differing in bacterial biomass (7.5 × 10<sup>6</sup>, 7.5 × 10<sup>7</sup>, and 7.5 × 10<sup>8</sup> cells/g-soil). The rate of bioplastic degradation was enhanced accompanied with an increase of the bacterial biomass in soil. 16S rDNA PCR-DGGE analysis indicated that the bacterial diversity in the soil was not affected by the degradation of bioplastics. Moreover, the degradation of bioplastic did not affect the nitrogen circulation activity in the soil.展开更多
The focus of environmental regulations has changed significantly since the introduction of the bioassay as a standard means of assessing environmental impact.Prominent in this change is an increasing emphasis on prote...The focus of environmental regulations has changed significantly since the introduction of the bioassay as a standard means of assessing environmental impact.Prominent in this change is an increasing emphasis on protecting the integrity of natural ecosystems,which incorporate community-and system-level properties as well as organismal and population processes.Consequently,support for the use of multispecies testing has widened to include not only ecologists in academia but environmental scientists in the regulatory and industrial sector as well.The reason for this trend is clear:the additional environmental realism gained from tests utilizing communities of organisms allows for greater insight into the potential hazard of chemicals and other forms of human activity to natural ecosystems that cannot be obtained from single species tests alone.Many of the problems cited for multispecies testing early in their evolution as a hazard assessment tool have been refuted or overcome.In particular,the use of natural microbial communities minimizes several shortcomings typically associated with multispecies toxicity testing.This discussion includes the utility of microcosm and mesocosm tests using aquatic microbial communities as hazard assessment tools in conjunction with accumulating information on their performance in toxicity testing protocols.An increasing body of experimental evidence supports an expansion in the use of these tests for a variety of regulatory and research purposes.A shift in research focus is needed,however,to answer remaining questions and further refine standard protocols for these valuable ecotoxicological tools.展开更多
Background: Recently, the probable involvement of surfaces from the hospital environment as a disseminating source of resistant bacteria has been highlighted. The aim of the study was to compare resistant microorganis...Background: Recently, the probable involvement of surfaces from the hospital environment as a disseminating source of resistant bacteria has been highlighted. The aim of the study was to compare resistant microorganisms isolated from inanimate surfaces, equipments and patient blood culture samples in an Intensive Care Unit from Belo Horizonte, Brazil. Methods: A cross-sectional study was performed from July to October 2009. Data sources were microbiologic samples from environment and patient blood culture. Duplicate samples were obtained by swabs from up to seven different touch sites around two different patients in four different days. Jointly with the environmental samples, bacterial isolates from an adult ICU patients’ routine blood cultures were obtained from hospital laboratory. The samples were identified, tested for sensitivity and compared by rep-PCR test to verify similarity. Results: Difference among the averages of Colony Forming Units was found within the environment samples (p < 0.004). In the environment were identified antibiotic resistant microorganisms such as Vancomycin resistant Enterococcus faecalis, imipenem and ciprofloxacin Pseudomonas aeruginosa and multidrug-resistant Acinetobacter baumannii. Similarities (60% -80%) were established among environmental and blood culture samples. Conclusion: The environmental sampling showed different averages of contamination of the surfaces and equipment. The similarity among the bacterial isolates of patients’ blood cultures and environmental samples reinforces the hypothesis of the horizontal transference of pathogens.展开更多
Objective To investigate the baseline levels of microorganisms’growth on the hands of anesthesiologists and in the anesthesia environment at a cancer hospital.Methods This study performed in nine operating rooms and ...Objective To investigate the baseline levels of microorganisms’growth on the hands of anesthesiologists and in the anesthesia environment at a cancer hospital.Methods This study performed in nine operating rooms and among 25 anesthesiologists at a cancer hospital.Sampling of the hands of anesthesiologists and the anesthesia environment was performed at a ready-to-use operating room before patient contact began and after decontamination.Results Microorganisms’growth results showed that 20%(5/25)of anesthesiologists’hands carried microorganisms(>10 CFU/cm^(2))before patient contact began.Female anesthesiologists performed hand hygiene better than did their male counterparts,with fewer CFUs(P=0.0069)and fewer species(P=0.0202).Our study also found that 55.6%(5/9)of ready-to-use operating rooms carried microorganisms(>5 CFU/cm^(2)).Microorganisms regrowth began quickly(1 hour)after disinfection,and increased gradually over time,reaching the threshold at 4 hours after disinfection.Staphylococcus aureus was isolated from the hands of 20%(5/25)of anesthesiologists and 33.3%(3/9)of operating rooms.Conclusion Our study indicates that male anesthesiologists need to pay more attention to the standard operating procedures and effect evaluation of hand hygiene,daily cleaning rate of the operating room may be insufficient,and we would suggest that there should be a repeat cleaning every four hours.展开更多
Microorganisms and its metabolic types are diverse in Xinjiang because of those extreme/special environments of drought, high or low temperature, saline and alkaline, oligotrophy. This article reviewed the research pr...Microorganisms and its metabolic types are diverse in Xinjiang because of those extreme/special environments of drought, high or low temperature, saline and alkaline, oligotrophy. This article reviewed the research progress and achievements of various microbial resources (bacteria, fungi and actinomycete) in the special environments from the point of its ecology, development and application. Meanwhile, the development trend, protection and sustainable utilization of the microorganism resources were discussed.展开更多
[Objectives] The paper was to study the effects of probiotics on breeding environment of livestock and poultry and intestinal microorgan-isms of breeding animals. [Method] Chicken houses and hog houses were selected a...[Objectives] The paper was to study the effects of probiotics on breeding environment of livestock and poultry and intestinal microorgan-isms of breeding animals. [Method] Chicken houses and hog houses were selected as the research objects. The breeding house supplemented with basal diet was used as the control group;the breeding house sprayed with probiotics product 1 was experimental group 1(exogenous treatment);the basal diet mixed with probiotics product 2 and drinking water mixed with product 3 was experimental group 2(endogenous treatment);and the com-bination of the two experimental groups was experimental group 3. [Results] Experimental groups 1 and 2 reduced the concentration of ammonia in hog houses and chicken houses, and the effect of experimental group 2 was better than that of experimental group 1. Experimental group 3 had the best effect, which significantly reduced the ammonia concentration in hog houses and chicken houses by 28.67% and 34.09%, respectively, and the average ammonia concentration outside the house within the range of 100-300 m was less than 2 mg/m^(3). Moreover, it significantly increased the number of intestinal lactobacillus and reduced the number of Escherichia coli. [Conclusions] Probiotics can effectively reduce the ammonia concentration in livestock and poultry breeding environment, and improve the intestinal microbial structure of breeding animals. The technology of spraying exogenous probiotics combined with the use of content-derived probiotics has great potential for popularization and application.展开更多
Light levels determine regeneration in stands and a key concern is how to regulate the light environment of different stand types to the requirements of the understory.In this study,we selected three stands typical in...Light levels determine regeneration in stands and a key concern is how to regulate the light environment of different stand types to the requirements of the understory.In this study,we selected three stands typical in south China(a Cryptomeria japonica plantation,a Quercus acutissima plantation,and a mixed stand of both)and three thinning intensities to determine the best understory light environ-ment for 3-year-old Phoebe bournei seedlings.The canopy structure,understory light environment,and photosynthe-sis and growth indicators were assessed following thin-ning.Thinning improved canopy structure and understory light availability of each stand;species composition was the reason for differences in the understory light environ-ment.Under the same thinning intensity,the mixed stand had the greatest light radiation and most balanced spectral composition.P.bournei photosynthesis and growth were closely related to the light environment;all three stands required heavy thinning to create an effective and sustained understory light environment.In a suitable understory light environment,the efficiency of light interception,absorption,and use by seedlings was enhanced,resulting in a higher carbon assimilation the main limiting factor was stomatal conductance.As a shade-avoidance signal,red/far-red radia-tion is a critical factor driving changes in photosynthesis and growth of P.bournei seedlings,and a reduction increased light absorption and use capacity and height:diameter ratios.The growth advantage transformed from diameter to height,enabling seedlings to access more light.Our findings suggest that the regeneration of shade-tolerant species such as P.bournei could be enhanced if a targeted approach to thinning based on stand type was adopted.展开更多
Purpose: The genus Pseudomonas is a ubiquitous microorganism frequently detected from immunocompromised patients. The inherent resistance to numerous antimicrobial agents contributes to the opportunistic character of ...Purpose: The genus Pseudomonas is a ubiquitous microorganism frequently detected from immunocompromised patients. The inherent resistance to numerous antimicrobial agents contributes to the opportunistic character of this pathogen exhaustive monitoring of this pathogen is considered of critical importance to public health organizations. The reliable identification method able to distinguish genetic close Pseudomonas species is needed, because these organisms are difficult to differentiate by phenotypic or biochemical methods. The purpose of the present study was to design species-specific primers in order to identify and detect four Pseudomonas species which are frequently detected from the human oral cavities, and to investigate the distribution of these organisms in the living environment using a multiplex PCR. Methods: Polymerase chain reaction (PCR) primers were designed based on partial sequences of the rpoD gene of four Pseudomonas species. Swab samples were collected from fifty washstands, and the distribution of Pseudomonas species was investigated using a conventional PCR at genus level and a multiplex PCR at species level. Results: Multiplex PCR method developed in this study was able to distinguish four Pseudomonas species clearly. The genus Pseudomonas was detected from all samples (100%), whereas P. putida, P, aeruginosa, P. stutzeri and P. fluorescens were detected at 44%, 8%, 4% and 2% in fifty swab samples, respectively. Conclusion: Our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and works without requiring DNA extraction. It was indicated that washstands were the uninhabitable environment for P. putida, P, aeruginosa, P. stutzeri and P. fluorescens.展开更多
Carbon peak and carbon neutrality(dual-carbon)are important targets for the international response to climate change.The Silk Road Economic Belt is a strategic resource region and is important for future ecological en...Carbon peak and carbon neutrality(dual-carbon)are important targets for the international response to climate change.The Silk Road Economic Belt is a strategic resource region and is important for future ecological environment and tourism development.Based on the“dual-carbon”targets,the Single index quantification,Multiple index synthesis,and Poly-criteria integration evaluation model were used in this study to measure the coordinated development index of the ecological environment,public service,and tourism economy along the Silk Road Economic Belt and to analyze its spatial and temporal evolution.Further,it explores the dynamic evolution and development trend of the three systems using the Kernel Density and Grey Markov Prediction Model.The results show that the coordinated development index along this region needs to be improved during the study period.Furthermore,the coordinated development index of the Southwest region is relatively higher than that of the Northwest region.From the development trend of the three systems,all of them develop in a stable manner;however,the tourism economy system is easily affected by external disturbances.The coordinated development index of the three systems changes dynamically and tends to be in a good state of coordination.There is a certain spatial and temporal heterogeneity.The gravity center of the coordinated development index has been in the Southwest region.During the forecast period,the coordinated development index along this region will improve significantly,while insufficient and unbalanced development will continue.展开更多
BACKGROUND Preschoolers become anxious when they are about to undergo anesthesia and surgery,warranting the development of more appropriate and effective interventions.AIM To explore the effect of static cartoons comb...BACKGROUND Preschoolers become anxious when they are about to undergo anesthesia and surgery,warranting the development of more appropriate and effective interventions.AIM To explore the effect of static cartoons combined with dynamic virtual environments on preoperative anxiety and anesthesia induction compliance in preschool-aged children undergoing surgery.METHODS One hundred and sixteen preschool-aged children were selected and assigned to the drug(n=37),intervention(n=40),and control(n=39)groups.All the children received routine preoperative checkups and nursing before being transferred to the preoperative preparation room on the day of the operation.The drug group received 0.5 mg/kg midazolam and the intervention group treatment consisting of static cartoons combined with dynamic virtual environments.The control group received no intervention.The modified Yale Preoperative Anxiety Scale was used to evaluate the children’s anxiety level on the day before surgery(T0),before leaving the preoperative preparation room(T1),when entering the operating room(T2),and at anesthesia induction(T3).Compliance during anesthesia induction(T3)was evaluated using the Induction Compliance Checklist(ICC).Changes in mean arterial pressure(MAP),heart rate(HR),and respiratory rate(RR)were also recorded at each time point.RESULTS The anxiety scores of the three groups increased variously at T1 and T2.At T3,both the drug and intervention groups had similar anxiety scores,both of which were lower than those in the control group.At T1 and T2,MAP,HR,and RR of the three groups increased.The drug and control groups had significantly higher MAP and RR than the intervention group at T2.At T3,the MAP,HR,and RR of the drug group decreased and were significantly lower than those in the control group but were comparable to those in the intervention group.Both the drug and intervention groups had similar ICC scores and duration of anesthesia induction(T3),both of which were higher than those of the control group.CONCLUSION Combining static cartoons with dynamic virtual environments as effective as medication,specifically midazolam,in reducing preoperative anxiety and fear in preschool-aged children.This approach also improve their compliance during anesthesia induction and helped maintain their stable vital signs.展开更多
High velocity oxygen fuel(HVOF)spraying process is commonly used to produce superalloy coatings.Inconel 625 coating was prepared on Q235B low carbon steel by HVOF.A series of experiments were conducted to examine the ...High velocity oxygen fuel(HVOF)spraying process is commonly used to produce superalloy coatings.Inconel 625 coating was prepared on Q235B low carbon steel by HVOF.A series of experiments were conducted to examine the surface and corrosion resistance properties of Inconel 625 HVOF coating.In this paper,potentiodynamic polarization tests and electrochemical impedance spectroscopy(EIS)tests were carried out to evaluate the corrosion resistance of Inconel 625 coating under simulated marine environment.The experiment-al results showed that Inconel 625 coating revealed low porosity and desired coating thickness.Shift in the corrosion potential(E_(corr))to-wards the noble direction combined with much low corrosion current density(i_(corr))indicating a significant improvement of HVOF Inconel 625 coating compared with the substrate.展开更多
Antarctic Peninsula is experiencing one of the largest global warming events worldwide.Shallow water bodies generated by the melting of snow in summer are numerous,and they might act as sentinels of climate change due...Antarctic Peninsula is experiencing one of the largest global warming events worldwide.Shallow water bodies generated by the melting of snow in summer are numerous,and they might act as sentinels of climate change due to their rapid response and ability to integrate catchment information.Shifts in climate can influence the structure of microbial communities which dominate these freshwaters ecosystems.Here,we characterize three ponds at Cierva Point(Antarctic Peninsula)by examining their physico-chemical and morphological characteristics and we explored how different factors modify the structure of the microbial community.We studied the abundance and biomass of heterotrophic bacteria,picocyanobacteria and picoeukaryote algae during January and February of two consecutive summers(2017 and 2018).We found that ponds had different limnological characteristics,due to their location,geomorphological features and presence of the surrounding flora and fauna.Physico-chemical parameters as well as microbial community differed between ponds,months and years.In 2017,most ponds were oligo to mesotrophic states.The larger accumulated rainfall(as a result of environmental changes on the Antarctic Peninsula)during 2018,particularly in February,causes nutrient runoff into water bodies.This affects those ponds with the highest seabird circulation,such as gentoo penguin,increasing eutrophication.As a result,picoplanktonic abundances were higher,and the community structure shifts to a largely heterotrophic bacteria dominated one.These results suggest that these communities could act as sentinels to environmental changes,anticipating a future with mostly hypertrophic ponds.展开更多
Smart Industrial environments use the Industrial Internet of Things(IIoT)for their routine operations and transform their industrial operations with intelligent and driven approaches.However,IIoT devices are vulnerabl...Smart Industrial environments use the Industrial Internet of Things(IIoT)for their routine operations and transform their industrial operations with intelligent and driven approaches.However,IIoT devices are vulnerable to cyber threats and exploits due to their connectivity with the internet.Traditional signature-based IDS are effective in detecting known attacks,but they are unable to detect unknown emerging attacks.Therefore,there is the need for an IDS which can learn from data and detect new threats.Ensemble Machine Learning(ML)and individual Deep Learning(DL)based IDS have been developed,and these individual models achieved low accuracy;however,their performance can be improved with the ensemble stacking technique.In this paper,we have proposed a Deep Stacked Neural Network(DSNN)based IDS,which consists of two stacked Convolutional Neural Network(CNN)models as base learners and Extreme Gradient Boosting(XGB)as the meta learner.The proposed DSNN model was trained and evaluated with the next-generation dataset,TON_IoT.Several pre-processing techniques were applied to prepare a dataset for the model,including ensemble feature selection and the SMOTE technique.Accuracy,precision,recall,F1-score,and false positive rates were used to evaluate the performance of the proposed ensemble model.Our experimental results showed that the accuracy for binary classification is 99.61%,which is better than in the baseline individual DL and ML models.In addition,the model proposed for IDS has been compared with similar models.The proposed DSNN achieved better performance metrics than the other models.The proposed DSNN model will be used to develop enhanced IDS for threat mitigation in smart industrial environments.展开更多
Objective:To evaluate the protective effects of probiotic microorganisms on the reproductive and nervous systems of male rats treated with acrylamide.Methods:Thirty-two rats were randomly divided into 4 groups and rec...Objective:To evaluate the protective effects of probiotic microorganisms on the reproductive and nervous systems of male rats treated with acrylamide.Methods:Thirty-two rats were randomly divided into 4 groups and received normal saline through gavage(control),acrylamide 20 mg/kg body weight,acrylamide plus probiotic microorganisms(Lactobacillus acidophilus,Lactobacillus casei,Lactobacillus bulgaricus,Lactobacillus rhamnosus,Bifidobacterium breve,Bifidobacterium infantis,Streptococcus thermophilus and fructooligosaccharides,all mixed in sachets)20 or 200 mg/kg body weight,respectively.After 30 days,the testis,prostate,seminal vesicle and cerebellum were removed,fixed and stained with hematoxylin-eosin(H&E).The Johnsen score was used to classify spermatogenesis.Cavalieri's principle method was used to evaluate the total volume(in mm3)of the testes.The number of each intratubular cell type as well as intertubular Leydig cells in whole samples was measured using the physical dissector counting techniques.Stereological analysis and the grids were used to determine the volume of cerebellar layers as well as the Purkinje cell number.Results:The testis weight decreased significantly in the acrylamide-treated group compared to the other groups(P<0.001).The number of spermatogonia,spermatocytes,spermatids and Leydig cells in the acrylamide-treated group were significantly less compared to the control group(P<0.05),while they were increased significantly in the acrylamide+200 mg/kg probiotic group(P<0.05;P<0.01).The mean Johnsen score in the acrylamide-treated group was lower than in the control group(P<0.001).Acrylamide-induced changes including congestion,vacuolization in the secretory epithelial cells,and epithelial rupture were observed in the prostate and seminal vesicle.The volumes of cerebellar layers were decreased in the acrylamide group compared to the control group while recovered in both probiotic treated groups.Conclusions:Probiotic microorganisms alleviate acrylamide-induced toxicities against the reproductive and cerebellar tissues in rats.展开更多
12%difenoconazole+fluxapyroxad SC(commercial name:Jiangong)was first released by BASF in China in 2016.It has been registered to control many diseases,including pear scab,apple Alternaria leaf spot,tomato early blight...12%difenoconazole+fluxapyroxad SC(commercial name:Jiangong)was first released by BASF in China in 2016.It has been registered to control many diseases,including pear scab,apple Alternaria leaf spot,tomato early blight,cucumber powdery mildew,etc.This study evaluated the bioactivity of Jiangong against Alternaria alternata and explored variations of phyllosphere microorganisms in both asymptomatic and tobacco brown spot leaves at different persistence periods(0,5,10,and 15 days post-fungicide application)using high-throughput sequencing technology.The results indicated that Jiangong effectively inhibited mycelial growth(average EC_(50) value of 0.51μg/mL),conidia germination(average EC_(50) value of 3.47μg/mL),and the carbon metabolism of A.alternata.Both asymptomatic and symptomatic leaves presented complex microbial communities.Higher fungal diversity was noted in asymptomatic leaves,while higher bacterial diversity was found in symptomatic leaves.After application,the diversity and abundance of microbial community structures in both types of leaves changed over time.Fungal microbiome communities showed greater sensitivity than bacterial groups,with the microbiome communities of asymptomatic leaves being more affected than those of symptomatic leaves.Fungal community diversity decreased for both symptomatic and asymptomatic leaves after 5 days of application,while the diversity of fungal community in symptomatic leaves showed an upward trend after 10 days of application.Meanwhile,bacterial community diversity increased in both symptomatic and asymptomatic leaves after 5 days of application but then declined in asymptomatic leaves after 15 days.The abundance of the dominant function group of phyllosphere bacteria(metabolism,genetic information processing,environmental information processing)was not affected by the application of Jiangong.However,the abundance of the dominant function group of phyllosphere fungi(animal pathogen-endophyte-wood saprotroph,endophyte-plant pathogen,plant pathogen-undefined saprotroph)was significantly affected by the application of Jiangong,and high variation was found in symptomatic leaves than that of asymptomatic leaves.The application of Jiangong-induced alterations in the community structure of the tobacco phyllosphere microbiome provides a basis for future tobacco brown spot control strategies based on phyllospheric microecology.展开更多
The primary objective of this study was to design and size a sustainable sanitation solution for the Ndiebene Gandiol 1 school located in the eponymous commune in northern Senegal. Field investigations led to the coll...The primary objective of this study was to design and size a sustainable sanitation solution for the Ndiebene Gandiol 1 school located in the eponymous commune in northern Senegal. Field investigations led to the collection of wastewater samples. Their analysis revealed specific pollutant loads, including loads of BOD5 3.6966 kgO<sub>2</sub>/day and COD of 12.8775 kgO<sub>2</sub>/day, which were central to the design phase. Following a rigorous assessment of the existing sanitation infrastructure, constructed wetland (CWs) emerged as the most appropriate ecological solution. This system, valued for its ability to effectively remove contaminants, was tailored to the specific needs of the site. Consequently, the final design of the filter extends over 217.16 m<sup>2</sup>, divided into two cells of 108.58 m<sup>2</sup> each, with dimensions of 12.77 m in length and 8.5 m in width. The depth of the filtering medium is approximately 0.60 m, meeting the standards while ensuring maximized purification. Typha, an indigenous and prolific plant known for its purification abilities, was selected as the filtering agent. Concurrently, non-crushed gravel was chosen for its proven filtration capacity. This study is the result of a combination of scientific rigor and design expertise. It provides a holistic view of sanitation for Ndiebene Gandiol. The technical specifications and dimensions of the constructed wetland filter embody an approach that marries indepth analysis and practical application, all aimed at delivering an effective and long-lasting solution to the local sanitation challenges. By integrating precise scientific data with sanitation design expertise, this study delivers a holistic solution for Ndiebene Gandiol. The detailed dimensions and specifications of the constructed wetland filter reflect a methodology that combines meticulous analysis with practical adaptation, aiming to provide an effective and sustainable response to the challenges of rural and school sanitation in the northern region of Senegal.展开更多
Upper Ordovician-Lower Silurian Wufeng-Longmaxi Formation is the most developed strata of shale gas in southern China.Due to the complex sedimentary environment adjacent to the Kangdian Uplift,the favorable area for o...Upper Ordovician-Lower Silurian Wufeng-Longmaxi Formation is the most developed strata of shale gas in southern China.Due to the complex sedimentary environment adjacent to the Kangdian Uplift,the favorable area for organic-rich shale development is still undetermined.The authors,therefore,focus on the mechanism of accumulation of organic matter and the characterization of the sedimentary environment of the Wufeng-Longmaxi Shales to have a more complete understanding and new discovering of organic matter enrichment and favorable area in the marginal region around Sichuan Basin.Multiple methods were applied in this study,including thin section identification,scanning electron microscopy(SEM)observations and X-ray diffraction(XRD),and elemental analysis on outcrop samples.Five lithofacies have been defined according to the mineralogical and petrological analyses,including mudstone,bioclastic limestone,silty shale,dolomitic shale,and carbonaceous siliceous shale.The paleo-environments have been reconstructed and the organic enrichment mechanism has been identified as a reduced environment and high productivity.The Wufeng period is generally a suboxic environment and the early Longmaxi period is a reducing environment based on geochemical characterization.High dolomite content in the study area is accompanied by high TOC,which may potentially indicate the restricted anoxic environment formed by biological flourishing in shallower water.And for the area close to the Kangdian Uplift,the shale gas generation capability is comparatively favorable.The geochemical parameters implied that new favorable areas for shale gas exploration could be targeted,and more shale gas resources in the mountain-basin transitional zone might be identified in the future.展开更多
The regulating nitrogen content of diamond in a hydrogen-rich high-temperature and high-pressure(HPHT) growth environment was systematically investigated in this work by developing three growth systems,namely, "F...The regulating nitrogen content of diamond in a hydrogen-rich high-temperature and high-pressure(HPHT) growth environment was systematically investigated in this work by developing three growth systems,namely, "FeNi+Ti", "FeNi+G_(3)N_(6)H_(6)",and "FeNi+Ti+C_(3)N_(6)H_(6)".Optical microscopy,infrared spectroscopy,and photoluminescence(PL)spectroscopy measurements were conducted to analyze the spectroscopic characteristics of diamonds grown in these three systems.From our analysis,it was demonstrated that the presence of hydrogen in the sp^(3) hybrid C-H does not directly affect the color of the diamond and facilitates the increase of the nitrogen-vacancy(NV) center concentration in a highnitrogen-content diamond.In addition,titanium plays an important role in nitrogen removal,while its impact on hydrogen doping within the diamond lattice is insignificant.Most importantly,by regulating the ratio of nitrogen impurities that coexist in the nitrogen and hydrogen HPHT environment,the production of hydrogenous Ⅱa-type diamond,hydrogenous Ib-type diamond,and hydrogenous high-nitrogen-type diamonds was achieved with a nitrogen content of less than 1 ppm to 1600 ppm.展开更多
Salinized soil is an important reserved arable land resource in China.The management and utilization of salinized soil can safeguard the current size of arable land and a stable grain yield.Salt accumulation will lead...Salinized soil is an important reserved arable land resource in China.The management and utilization of salinized soil can safeguard the current size of arable land and a stable grain yield.Salt accumulation will lead to the deterioration of soil properties,destroy soil production potential and damage soil ecological functions,which in turn will threaten global water and soil resources and food security,and affect sustainable socio-economic development.Microorganisms are important components of salinized soil.Microbial remediation is an important research tool in improving salinized soil and is key to realizing sustainable development of agriculture and the ecosystem.Knowledge about the impact of salinization on soil properties and measures using microorganisms in remediation of salinized soil has grown over time.However,the mechanisms governing these impacts and the ecological principles for microbial remediation are scarce.Thus,it is imperative to summarize the effects of salinization on soil physical,chemical,and microbial properties,and then review the related mechanisms of halophilic and halotolerant microorganisms in salinized soil remediation via direct and indirect pathways.The stability,persistence,and safety of the microbial remediation effect is also highlighted in this review to further promote the application of microbial remediation in salinized soil.The objective of this review is to provide reference and theoretical support for the improvement and utilization of salinized soil.展开更多
The ocean is one of the essential fields of national defense in the future,and more and more attention is paid to the lightweight research of Marine equipment and materials.This study it is to develop a Machine learni...The ocean is one of the essential fields of national defense in the future,and more and more attention is paid to the lightweight research of Marine equipment and materials.This study it is to develop a Machine learning(ML)-based prediction method to study the evolution of the mechanical properties of Al-Li alloys in the marine environment.We obtained the mechanical properties of Al-Li alloy samples under uniaxial tensile deformation at different exposure times through Marine exposure experiments.We obtained the strain evolution by digital image correlation(DIC).The strain field images are voxelized using 2D-Convolutional Neural Networks(CNN)autoencoders as input data for Long Short-Term Memory(LSTM)neural networks.Then,the output data of LSTM neural networks combined with corrosion features were input into the Back Propagation(BP)neural network to predict the mechanical properties of Al-Li alloys.The main conclusions are as follows:1.The variation law of mechanical properties of2297-T8 in the Marine atmosphere is revealed.With the increase in outdoor exposure test time,the tensile elastic model of 2297-T8 changes slowly,within 10%,and the tensile yield stress changes significantly,with a maximum attenuation of 23.6%.2.The prediction model can predict the strain evolution and mechanical response simultaneously with an error of less than 5%.3.This study shows that a CNN/LSTM system based on machine learning can be built to capture the corrosion characteristics of Marine exposure experiments.The results show that the relationship between corrosion characteristics and mechanical response can be predicted without considering the microstructure evolution of metal materials.展开更多
文摘Degradation of three kinds of bioplastics and their effects on microbial biomass and microbial diversity in soil environment were analyzed. The degradation rate of bioplastic in soil was closely related to the main components in the bioplastics. Poly (butylene succinate)-starch (PBS-starch) and poly (butylene succinate) (PBS) were degraded by 1% to 7% after 28 days in a soil with an initial bacterial biomass of 1.4 × 10<sup>9</sup> cells/g-soil, however poly lactic acid (PLA) was not degraded in the soil after 28 days. When the powdered-bioplastics were examined for the degradation in the soil, PBS-starch also showed the highest degradability (24.4% degradation after 28 days), and the similar results were obtained in the case of long-term degradation experiment (2 years). To investigate the effect of bacterial biomass in soil on biodegradability of bioplastics, PBS-starch was buried in three kinds of soils differing in bacterial biomass (7.5 × 10<sup>6</sup>, 7.5 × 10<sup>7</sup>, and 7.5 × 10<sup>8</sup> cells/g-soil). The rate of bioplastic degradation was enhanced accompanied with an increase of the bacterial biomass in soil. 16S rDNA PCR-DGGE analysis indicated that the bacterial diversity in the soil was not affected by the degradation of bioplastics. Moreover, the degradation of bioplastic did not affect the nitrogen circulation activity in the soil.
基金This manuscript and specific ex periments discussed within were sponsored,in part,by grants from The Procter&Gamble Company and the du Pont Educational Foundation,altbough the authors take full responsibility for the views ex-pressed herein.
文摘The focus of environmental regulations has changed significantly since the introduction of the bioassay as a standard means of assessing environmental impact.Prominent in this change is an increasing emphasis on protecting the integrity of natural ecosystems,which incorporate community-and system-level properties as well as organismal and population processes.Consequently,support for the use of multispecies testing has widened to include not only ecologists in academia but environmental scientists in the regulatory and industrial sector as well.The reason for this trend is clear:the additional environmental realism gained from tests utilizing communities of organisms allows for greater insight into the potential hazard of chemicals and other forms of human activity to natural ecosystems that cannot be obtained from single species tests alone.Many of the problems cited for multispecies testing early in their evolution as a hazard assessment tool have been refuted or overcome.In particular,the use of natural microbial communities minimizes several shortcomings typically associated with multispecies toxicity testing.This discussion includes the utility of microcosm and mesocosm tests using aquatic microbial communities as hazard assessment tools in conjunction with accumulating information on their performance in toxicity testing protocols.An increasing body of experimental evidence supports an expansion in the use of these tests for a variety of regulatory and research purposes.A shift in research focus is needed,however,to answer remaining questions and further refine standard protocols for these valuable ecotoxicological tools.
文摘Background: Recently, the probable involvement of surfaces from the hospital environment as a disseminating source of resistant bacteria has been highlighted. The aim of the study was to compare resistant microorganisms isolated from inanimate surfaces, equipments and patient blood culture samples in an Intensive Care Unit from Belo Horizonte, Brazil. Methods: A cross-sectional study was performed from July to October 2009. Data sources were microbiologic samples from environment and patient blood culture. Duplicate samples were obtained by swabs from up to seven different touch sites around two different patients in four different days. Jointly with the environmental samples, bacterial isolates from an adult ICU patients’ routine blood cultures were obtained from hospital laboratory. The samples were identified, tested for sensitivity and compared by rep-PCR test to verify similarity. Results: Difference among the averages of Colony Forming Units was found within the environment samples (p < 0.004). In the environment were identified antibiotic resistant microorganisms such as Vancomycin resistant Enterococcus faecalis, imipenem and ciprofloxacin Pseudomonas aeruginosa and multidrug-resistant Acinetobacter baumannii. Similarities (60% -80%) were established among environmental and blood culture samples. Conclusion: The environmental sampling showed different averages of contamination of the surfaces and equipment. The similarity among the bacterial isolates of patients’ blood cultures and environmental samples reinforces the hypothesis of the horizontal transference of pathogens.
基金supported by the Young Scientific Research Fund of PUMCH(Peking Union Medical College Hospital)[Grant No.pumch201911291]。
文摘Objective To investigate the baseline levels of microorganisms’growth on the hands of anesthesiologists and in the anesthesia environment at a cancer hospital.Methods This study performed in nine operating rooms and among 25 anesthesiologists at a cancer hospital.Sampling of the hands of anesthesiologists and the anesthesia environment was performed at a ready-to-use operating room before patient contact began and after decontamination.Results Microorganisms’growth results showed that 20%(5/25)of anesthesiologists’hands carried microorganisms(>10 CFU/cm^(2))before patient contact began.Female anesthesiologists performed hand hygiene better than did their male counterparts,with fewer CFUs(P=0.0069)and fewer species(P=0.0202).Our study also found that 55.6%(5/9)of ready-to-use operating rooms carried microorganisms(>5 CFU/cm^(2)).Microorganisms regrowth began quickly(1 hour)after disinfection,and increased gradually over time,reaching the threshold at 4 hours after disinfection.Staphylococcus aureus was isolated from the hands of 20%(5/25)of anesthesiologists and 33.3%(3/9)of operating rooms.Conclusion Our study indicates that male anesthesiologists need to pay more attention to the standard operating procedures and effect evaluation of hand hygiene,daily cleaning rate of the operating room may be insufficient,and we would suggest that there should be a repeat cleaning every four hours.
基金supported by the National Natural Science Foundation of Xinjiang (200821161)the Key Knowledge Innovation Project of Chinese Academy of Sciences (KZCX2-YW-336-2)the National Support Plan of China (2007BAC15B06)
文摘Microorganisms and its metabolic types are diverse in Xinjiang because of those extreme/special environments of drought, high or low temperature, saline and alkaline, oligotrophy. This article reviewed the research progress and achievements of various microbial resources (bacteria, fungi and actinomycete) in the special environments from the point of its ecology, development and application. Meanwhile, the development trend, protection and sustainable utilization of the microorganism resources were discussed.
基金Supported by Instructive Project of Fujian Provincial Science and Technology Plan(2019Y6005)。
文摘[Objectives] The paper was to study the effects of probiotics on breeding environment of livestock and poultry and intestinal microorgan-isms of breeding animals. [Method] Chicken houses and hog houses were selected as the research objects. The breeding house supplemented with basal diet was used as the control group;the breeding house sprayed with probiotics product 1 was experimental group 1(exogenous treatment);the basal diet mixed with probiotics product 2 and drinking water mixed with product 3 was experimental group 2(endogenous treatment);and the com-bination of the two experimental groups was experimental group 3. [Results] Experimental groups 1 and 2 reduced the concentration of ammonia in hog houses and chicken houses, and the effect of experimental group 2 was better than that of experimental group 1. Experimental group 3 had the best effect, which significantly reduced the ammonia concentration in hog houses and chicken houses by 28.67% and 34.09%, respectively, and the average ammonia concentration outside the house within the range of 100-300 m was less than 2 mg/m^(3). Moreover, it significantly increased the number of intestinal lactobacillus and reduced the number of Escherichia coli. [Conclusions] Probiotics can effectively reduce the ammonia concentration in livestock and poultry breeding environment, and improve the intestinal microbial structure of breeding animals. The technology of spraying exogenous probiotics combined with the use of content-derived probiotics has great potential for popularization and application.
基金This study was supported by the National Natural Science Foundation of China(Grant No.31870613)Guizhou Province High-level Innovative Talents Training Plan Project(2016)5661.
文摘Light levels determine regeneration in stands and a key concern is how to regulate the light environment of different stand types to the requirements of the understory.In this study,we selected three stands typical in south China(a Cryptomeria japonica plantation,a Quercus acutissima plantation,and a mixed stand of both)and three thinning intensities to determine the best understory light environ-ment for 3-year-old Phoebe bournei seedlings.The canopy structure,understory light environment,and photosynthe-sis and growth indicators were assessed following thin-ning.Thinning improved canopy structure and understory light availability of each stand;species composition was the reason for differences in the understory light environ-ment.Under the same thinning intensity,the mixed stand had the greatest light radiation and most balanced spectral composition.P.bournei photosynthesis and growth were closely related to the light environment;all three stands required heavy thinning to create an effective and sustained understory light environment.In a suitable understory light environment,the efficiency of light interception,absorption,and use by seedlings was enhanced,resulting in a higher carbon assimilation the main limiting factor was stomatal conductance.As a shade-avoidance signal,red/far-red radia-tion is a critical factor driving changes in photosynthesis and growth of P.bournei seedlings,and a reduction increased light absorption and use capacity and height:diameter ratios.The growth advantage transformed from diameter to height,enabling seedlings to access more light.Our findings suggest that the regeneration of shade-tolerant species such as P.bournei could be enhanced if a targeted approach to thinning based on stand type was adopted.
文摘Purpose: The genus Pseudomonas is a ubiquitous microorganism frequently detected from immunocompromised patients. The inherent resistance to numerous antimicrobial agents contributes to the opportunistic character of this pathogen exhaustive monitoring of this pathogen is considered of critical importance to public health organizations. The reliable identification method able to distinguish genetic close Pseudomonas species is needed, because these organisms are difficult to differentiate by phenotypic or biochemical methods. The purpose of the present study was to design species-specific primers in order to identify and detect four Pseudomonas species which are frequently detected from the human oral cavities, and to investigate the distribution of these organisms in the living environment using a multiplex PCR. Methods: Polymerase chain reaction (PCR) primers were designed based on partial sequences of the rpoD gene of four Pseudomonas species. Swab samples were collected from fifty washstands, and the distribution of Pseudomonas species was investigated using a conventional PCR at genus level and a multiplex PCR at species level. Results: Multiplex PCR method developed in this study was able to distinguish four Pseudomonas species clearly. The genus Pseudomonas was detected from all samples (100%), whereas P. putida, P, aeruginosa, P. stutzeri and P. fluorescens were detected at 44%, 8%, 4% and 2% in fifty swab samples, respectively. Conclusion: Our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and works without requiring DNA extraction. It was indicated that washstands were the uninhabitable environment for P. putida, P, aeruginosa, P. stutzeri and P. fluorescens.
基金supported by the Hebei Province Cultural and Artistic Science Planning and Tourism Research Project[Grant No.HB22-ZD002].
文摘Carbon peak and carbon neutrality(dual-carbon)are important targets for the international response to climate change.The Silk Road Economic Belt is a strategic resource region and is important for future ecological environment and tourism development.Based on the“dual-carbon”targets,the Single index quantification,Multiple index synthesis,and Poly-criteria integration evaluation model were used in this study to measure the coordinated development index of the ecological environment,public service,and tourism economy along the Silk Road Economic Belt and to analyze its spatial and temporal evolution.Further,it explores the dynamic evolution and development trend of the three systems using the Kernel Density and Grey Markov Prediction Model.The results show that the coordinated development index along this region needs to be improved during the study period.Furthermore,the coordinated development index of the Southwest region is relatively higher than that of the Northwest region.From the development trend of the three systems,all of them develop in a stable manner;however,the tourism economy system is easily affected by external disturbances.The coordinated development index of the three systems changes dynamically and tends to be in a good state of coordination.There is a certain spatial and temporal heterogeneity.The gravity center of the coordinated development index has been in the Southwest region.During the forecast period,the coordinated development index along this region will improve significantly,while insufficient and unbalanced development will continue.
基金Supported by Hangzhou Medical and Health Technology Project,No.OO20191141。
文摘BACKGROUND Preschoolers become anxious when they are about to undergo anesthesia and surgery,warranting the development of more appropriate and effective interventions.AIM To explore the effect of static cartoons combined with dynamic virtual environments on preoperative anxiety and anesthesia induction compliance in preschool-aged children undergoing surgery.METHODS One hundred and sixteen preschool-aged children were selected and assigned to the drug(n=37),intervention(n=40),and control(n=39)groups.All the children received routine preoperative checkups and nursing before being transferred to the preoperative preparation room on the day of the operation.The drug group received 0.5 mg/kg midazolam and the intervention group treatment consisting of static cartoons combined with dynamic virtual environments.The control group received no intervention.The modified Yale Preoperative Anxiety Scale was used to evaluate the children’s anxiety level on the day before surgery(T0),before leaving the preoperative preparation room(T1),when entering the operating room(T2),and at anesthesia induction(T3).Compliance during anesthesia induction(T3)was evaluated using the Induction Compliance Checklist(ICC).Changes in mean arterial pressure(MAP),heart rate(HR),and respiratory rate(RR)were also recorded at each time point.RESULTS The anxiety scores of the three groups increased variously at T1 and T2.At T3,both the drug and intervention groups had similar anxiety scores,both of which were lower than those in the control group.At T1 and T2,MAP,HR,and RR of the three groups increased.The drug and control groups had significantly higher MAP and RR than the intervention group at T2.At T3,the MAP,HR,and RR of the drug group decreased and were significantly lower than those in the control group but were comparable to those in the intervention group.Both the drug and intervention groups had similar ICC scores and duration of anesthesia induction(T3),both of which were higher than those of the control group.CONCLUSION Combining static cartoons with dynamic virtual environments as effective as medication,specifically midazolam,in reducing preoperative anxiety and fear in preschool-aged children.This approach also improve their compliance during anesthesia induction and helped maintain their stable vital signs.
基金supported by Zhejiang Provincial Natural Science Foundation of China(No.LTGC23E010001)the Youth Science and Technology Project of Zhejiang Provincial Administration for Market Regulation(No.QN2023427)Science and Techno-logy Project of State Administration for Market Regulation(No.2022MK054).
文摘High velocity oxygen fuel(HVOF)spraying process is commonly used to produce superalloy coatings.Inconel 625 coating was prepared on Q235B low carbon steel by HVOF.A series of experiments were conducted to examine the surface and corrosion resistance properties of Inconel 625 HVOF coating.In this paper,potentiodynamic polarization tests and electrochemical impedance spectroscopy(EIS)tests were carried out to evaluate the corrosion resistance of Inconel 625 coating under simulated marine environment.The experiment-al results showed that Inconel 625 coating revealed low porosity and desired coating thickness.Shift in the corrosion potential(E_(corr))to-wards the noble direction combined with much low corrosion current density(i_(corr))indicating a significant improvement of HVOF Inconel 625 coating compared with the substrate.
基金supported by ANPCy T (Grant PICT-2016-2517) directed by Dr. G. Matalonithe National Scientific and Technical Research Council-Argentina (CONICET)
文摘Antarctic Peninsula is experiencing one of the largest global warming events worldwide.Shallow water bodies generated by the melting of snow in summer are numerous,and they might act as sentinels of climate change due to their rapid response and ability to integrate catchment information.Shifts in climate can influence the structure of microbial communities which dominate these freshwaters ecosystems.Here,we characterize three ponds at Cierva Point(Antarctic Peninsula)by examining their physico-chemical and morphological characteristics and we explored how different factors modify the structure of the microbial community.We studied the abundance and biomass of heterotrophic bacteria,picocyanobacteria and picoeukaryote algae during January and February of two consecutive summers(2017 and 2018).We found that ponds had different limnological characteristics,due to their location,geomorphological features and presence of the surrounding flora and fauna.Physico-chemical parameters as well as microbial community differed between ponds,months and years.In 2017,most ponds were oligo to mesotrophic states.The larger accumulated rainfall(as a result of environmental changes on the Antarctic Peninsula)during 2018,particularly in February,causes nutrient runoff into water bodies.This affects those ponds with the highest seabird circulation,such as gentoo penguin,increasing eutrophication.As a result,picoplanktonic abundances were higher,and the community structure shifts to a largely heterotrophic bacteria dominated one.These results suggest that these communities could act as sentinels to environmental changes,anticipating a future with mostly hypertrophic ponds.
文摘Smart Industrial environments use the Industrial Internet of Things(IIoT)for their routine operations and transform their industrial operations with intelligent and driven approaches.However,IIoT devices are vulnerable to cyber threats and exploits due to their connectivity with the internet.Traditional signature-based IDS are effective in detecting known attacks,but they are unable to detect unknown emerging attacks.Therefore,there is the need for an IDS which can learn from data and detect new threats.Ensemble Machine Learning(ML)and individual Deep Learning(DL)based IDS have been developed,and these individual models achieved low accuracy;however,their performance can be improved with the ensemble stacking technique.In this paper,we have proposed a Deep Stacked Neural Network(DSNN)based IDS,which consists of two stacked Convolutional Neural Network(CNN)models as base learners and Extreme Gradient Boosting(XGB)as the meta learner.The proposed DSNN model was trained and evaluated with the next-generation dataset,TON_IoT.Several pre-processing techniques were applied to prepare a dataset for the model,including ensemble feature selection and the SMOTE technique.Accuracy,precision,recall,F1-score,and false positive rates were used to evaluate the performance of the proposed ensemble model.Our experimental results showed that the accuracy for binary classification is 99.61%,which is better than in the baseline individual DL and ML models.In addition,the model proposed for IDS has been compared with similar models.The proposed DSNN achieved better performance metrics than the other models.The proposed DSNN model will be used to develop enhanced IDS for threat mitigation in smart industrial environments.
基金Shahid Sadoughi University of Medical Sciences,Yazd,Iran(grant number 5689).
文摘Objective:To evaluate the protective effects of probiotic microorganisms on the reproductive and nervous systems of male rats treated with acrylamide.Methods:Thirty-two rats were randomly divided into 4 groups and received normal saline through gavage(control),acrylamide 20 mg/kg body weight,acrylamide plus probiotic microorganisms(Lactobacillus acidophilus,Lactobacillus casei,Lactobacillus bulgaricus,Lactobacillus rhamnosus,Bifidobacterium breve,Bifidobacterium infantis,Streptococcus thermophilus and fructooligosaccharides,all mixed in sachets)20 or 200 mg/kg body weight,respectively.After 30 days,the testis,prostate,seminal vesicle and cerebellum were removed,fixed and stained with hematoxylin-eosin(H&E).The Johnsen score was used to classify spermatogenesis.Cavalieri's principle method was used to evaluate the total volume(in mm3)of the testes.The number of each intratubular cell type as well as intertubular Leydig cells in whole samples was measured using the physical dissector counting techniques.Stereological analysis and the grids were used to determine the volume of cerebellar layers as well as the Purkinje cell number.Results:The testis weight decreased significantly in the acrylamide-treated group compared to the other groups(P<0.001).The number of spermatogonia,spermatocytes,spermatids and Leydig cells in the acrylamide-treated group were significantly less compared to the control group(P<0.05),while they were increased significantly in the acrylamide+200 mg/kg probiotic group(P<0.05;P<0.01).The mean Johnsen score in the acrylamide-treated group was lower than in the control group(P<0.001).Acrylamide-induced changes including congestion,vacuolization in the secretory epithelial cells,and epithelial rupture were observed in the prostate and seminal vesicle.The volumes of cerebellar layers were decreased in the acrylamide group compared to the control group while recovered in both probiotic treated groups.Conclusions:Probiotic microorganisms alleviate acrylamide-induced toxicities against the reproductive and cerebellar tissues in rats.
基金Supported by China National Tobacco Corporation[No.110202101048(LS-08)]Hundred’Level Innovative Talent Foundation of Guizhou Province(No.GCC[2022]028-1,GCC[2023]108)+2 种基金Guizhou Science Technology Foundation(No.ZK[2021]Key036)the National Natural Science Foundation of China(No.32160522)Guizhou Province Applied Technology Research and Development Funding Post-subsidy Project and Guizhou Tobacco Company(No.2020XM03,2020XM22,2024XM06).
文摘12%difenoconazole+fluxapyroxad SC(commercial name:Jiangong)was first released by BASF in China in 2016.It has been registered to control many diseases,including pear scab,apple Alternaria leaf spot,tomato early blight,cucumber powdery mildew,etc.This study evaluated the bioactivity of Jiangong against Alternaria alternata and explored variations of phyllosphere microorganisms in both asymptomatic and tobacco brown spot leaves at different persistence periods(0,5,10,and 15 days post-fungicide application)using high-throughput sequencing technology.The results indicated that Jiangong effectively inhibited mycelial growth(average EC_(50) value of 0.51μg/mL),conidia germination(average EC_(50) value of 3.47μg/mL),and the carbon metabolism of A.alternata.Both asymptomatic and symptomatic leaves presented complex microbial communities.Higher fungal diversity was noted in asymptomatic leaves,while higher bacterial diversity was found in symptomatic leaves.After application,the diversity and abundance of microbial community structures in both types of leaves changed over time.Fungal microbiome communities showed greater sensitivity than bacterial groups,with the microbiome communities of asymptomatic leaves being more affected than those of symptomatic leaves.Fungal community diversity decreased for both symptomatic and asymptomatic leaves after 5 days of application,while the diversity of fungal community in symptomatic leaves showed an upward trend after 10 days of application.Meanwhile,bacterial community diversity increased in both symptomatic and asymptomatic leaves after 5 days of application but then declined in asymptomatic leaves after 15 days.The abundance of the dominant function group of phyllosphere bacteria(metabolism,genetic information processing,environmental information processing)was not affected by the application of Jiangong.However,the abundance of the dominant function group of phyllosphere fungi(animal pathogen-endophyte-wood saprotroph,endophyte-plant pathogen,plant pathogen-undefined saprotroph)was significantly affected by the application of Jiangong,and high variation was found in symptomatic leaves than that of asymptomatic leaves.The application of Jiangong-induced alterations in the community structure of the tobacco phyllosphere microbiome provides a basis for future tobacco brown spot control strategies based on phyllospheric microecology.
文摘The primary objective of this study was to design and size a sustainable sanitation solution for the Ndiebene Gandiol 1 school located in the eponymous commune in northern Senegal. Field investigations led to the collection of wastewater samples. Their analysis revealed specific pollutant loads, including loads of BOD5 3.6966 kgO<sub>2</sub>/day and COD of 12.8775 kgO<sub>2</sub>/day, which were central to the design phase. Following a rigorous assessment of the existing sanitation infrastructure, constructed wetland (CWs) emerged as the most appropriate ecological solution. This system, valued for its ability to effectively remove contaminants, was tailored to the specific needs of the site. Consequently, the final design of the filter extends over 217.16 m<sup>2</sup>, divided into two cells of 108.58 m<sup>2</sup> each, with dimensions of 12.77 m in length and 8.5 m in width. The depth of the filtering medium is approximately 0.60 m, meeting the standards while ensuring maximized purification. Typha, an indigenous and prolific plant known for its purification abilities, was selected as the filtering agent. Concurrently, non-crushed gravel was chosen for its proven filtration capacity. This study is the result of a combination of scientific rigor and design expertise. It provides a holistic view of sanitation for Ndiebene Gandiol. The technical specifications and dimensions of the constructed wetland filter embody an approach that marries indepth analysis and practical application, all aimed at delivering an effective and long-lasting solution to the local sanitation challenges. By integrating precise scientific data with sanitation design expertise, this study delivers a holistic solution for Ndiebene Gandiol. The detailed dimensions and specifications of the constructed wetland filter reflect a methodology that combines meticulous analysis with practical adaptation, aiming to provide an effective and sustainable response to the challenges of rural and school sanitation in the northern region of Senegal.
基金jointly funded by the National Key Laboratory of Oil and Gas Reservoir Geology and Exploitation(PLC20210104)China Geological Survey(DD20221661)China National Science and Technology Major Project“Test and Application of Shale Gas Exploration and Evaluation Technology(2016ZX05034004)”。
文摘Upper Ordovician-Lower Silurian Wufeng-Longmaxi Formation is the most developed strata of shale gas in southern China.Due to the complex sedimentary environment adjacent to the Kangdian Uplift,the favorable area for organic-rich shale development is still undetermined.The authors,therefore,focus on the mechanism of accumulation of organic matter and the characterization of the sedimentary environment of the Wufeng-Longmaxi Shales to have a more complete understanding and new discovering of organic matter enrichment and favorable area in the marginal region around Sichuan Basin.Multiple methods were applied in this study,including thin section identification,scanning electron microscopy(SEM)observations and X-ray diffraction(XRD),and elemental analysis on outcrop samples.Five lithofacies have been defined according to the mineralogical and petrological analyses,including mudstone,bioclastic limestone,silty shale,dolomitic shale,and carbonaceous siliceous shale.The paleo-environments have been reconstructed and the organic enrichment mechanism has been identified as a reduced environment and high productivity.The Wufeng period is generally a suboxic environment and the early Longmaxi period is a reducing environment based on geochemical characterization.High dolomite content in the study area is accompanied by high TOC,which may potentially indicate the restricted anoxic environment formed by biological flourishing in shallower water.And for the area close to the Kangdian Uplift,the shale gas generation capability is comparatively favorable.The geochemical parameters implied that new favorable areas for shale gas exploration could be targeted,and more shale gas resources in the mountain-basin transitional zone might be identified in the future.
基金supported by the National Natural Science Foundation of China (Grant Nos. 12274373 and 12004341)the Open Project of Inner Mongolia Key Laboratory of High-pressure Phase Functional Materials,Chifeng University (Grant No. cfxygy202301)+1 种基金the Science and Technology Project of Xilinguole Province (Grant No. 202209)the Natural Science Foundation of Henan Province (Grant No. 242300421155)。
文摘The regulating nitrogen content of diamond in a hydrogen-rich high-temperature and high-pressure(HPHT) growth environment was systematically investigated in this work by developing three growth systems,namely, "FeNi+Ti", "FeNi+G_(3)N_(6)H_(6)",and "FeNi+Ti+C_(3)N_(6)H_(6)".Optical microscopy,infrared spectroscopy,and photoluminescence(PL)spectroscopy measurements were conducted to analyze the spectroscopic characteristics of diamonds grown in these three systems.From our analysis,it was demonstrated that the presence of hydrogen in the sp^(3) hybrid C-H does not directly affect the color of the diamond and facilitates the increase of the nitrogen-vacancy(NV) center concentration in a highnitrogen-content diamond.In addition,titanium plays an important role in nitrogen removal,while its impact on hydrogen doping within the diamond lattice is insignificant.Most importantly,by regulating the ratio of nitrogen impurities that coexist in the nitrogen and hydrogen HPHT environment,the production of hydrogenous Ⅱa-type diamond,hydrogenous Ib-type diamond,and hydrogenous high-nitrogen-type diamonds was achieved with a nitrogen content of less than 1 ppm to 1600 ppm.
基金the National Natural Science Foundation of China(No.42107513)the Key Projects of Natural Science Foundation of Gansu Province(No.22JR5RA051)+1 种基金the Gansu Province Science and Technology project(No.21JR7RA070)the Key Research and Development Program of Gansu Province(No.21YF5FA151).
文摘Salinized soil is an important reserved arable land resource in China.The management and utilization of salinized soil can safeguard the current size of arable land and a stable grain yield.Salt accumulation will lead to the deterioration of soil properties,destroy soil production potential and damage soil ecological functions,which in turn will threaten global water and soil resources and food security,and affect sustainable socio-economic development.Microorganisms are important components of salinized soil.Microbial remediation is an important research tool in improving salinized soil and is key to realizing sustainable development of agriculture and the ecosystem.Knowledge about the impact of salinization on soil properties and measures using microorganisms in remediation of salinized soil has grown over time.However,the mechanisms governing these impacts and the ecological principles for microbial remediation are scarce.Thus,it is imperative to summarize the effects of salinization on soil physical,chemical,and microbial properties,and then review the related mechanisms of halophilic and halotolerant microorganisms in salinized soil remediation via direct and indirect pathways.The stability,persistence,and safety of the microbial remediation effect is also highlighted in this review to further promote the application of microbial remediation in salinized soil.The objective of this review is to provide reference and theoretical support for the improvement and utilization of salinized soil.
基金supported by the Southwest Institute of Technology and Engineering cooperation fund(Grant No.HDHDW5902020104)。
文摘The ocean is one of the essential fields of national defense in the future,and more and more attention is paid to the lightweight research of Marine equipment and materials.This study it is to develop a Machine learning(ML)-based prediction method to study the evolution of the mechanical properties of Al-Li alloys in the marine environment.We obtained the mechanical properties of Al-Li alloy samples under uniaxial tensile deformation at different exposure times through Marine exposure experiments.We obtained the strain evolution by digital image correlation(DIC).The strain field images are voxelized using 2D-Convolutional Neural Networks(CNN)autoencoders as input data for Long Short-Term Memory(LSTM)neural networks.Then,the output data of LSTM neural networks combined with corrosion features were input into the Back Propagation(BP)neural network to predict the mechanical properties of Al-Li alloys.The main conclusions are as follows:1.The variation law of mechanical properties of2297-T8 in the Marine atmosphere is revealed.With the increase in outdoor exposure test time,the tensile elastic model of 2297-T8 changes slowly,within 10%,and the tensile yield stress changes significantly,with a maximum attenuation of 23.6%.2.The prediction model can predict the strain evolution and mechanical response simultaneously with an error of less than 5%.3.This study shows that a CNN/LSTM system based on machine learning can be built to capture the corrosion characteristics of Marine exposure experiments.The results show that the relationship between corrosion characteristics and mechanical response can be predicted without considering the microstructure evolution of metal materials.