Based on geometric moire method, moire interferometry and microscopic moire interferometry, a high spatial resolution and high sensitivity geometric microscopic moire method is presented. Geometric micron-moire patter...Based on geometric moire method, moire interferometry and microscopic moire interferometry, a high spatial resolution and high sensitivity geometric microscopic moire method is presented. Geometric micron-moire patterns are produced by the superposition of two high frequency gratings through a microscope system. Compared with other grating-based photo-mechanics methods, microscopic moire method could provide whole-field moire patterns of both high spatial resolution and high sensitivity. The frequency of specimen and reference gratings used in this method can be from 1 line/mm to 10000 lines/mm. Additionally, a 4F optical filter system is used to enhance the contrast of microscopic moire patterns effectively.展开更多
An error correction technique for the micro-scanning instrument of the optical micro-scanning thermal microscope imaging system is proposed. The technique is based on micro-scanning technology combined with the propos...An error correction technique for the micro-scanning instrument of the optical micro-scanning thermal microscope imaging system is proposed. The technique is based on micro-scanning technology combined with the proposed second-order oversampling reconstruction algorithm and local gradient image reconstruction algorithm. In this paper, we describe the local gradient image reconstruction model, the error correction technique, down-sampling model and the error correction principle. In this paper, we use a Lena original image and four low-resolution images obtained from the standard half-pixel displacement to simulate and verify the effectiveness of the proposed technique. In order to verify the effectiveness of the proposed technique, two groups of low-resolution thermal microscope images are collected by the actual thermal microscope imaging system for experimental study. Simulations and experiments show that the proposed technique can reduce the optical micro-scanning errors, improve the imaging effect of the system and improve the system's spatial resolution. It can be applied to other electro-optical imaging systems to improve their resolution.展开更多
The principle of laser optical feedback microscope was presented and demonstrated. Three methods to advance the vertical resolution of laser optical feedback microscope were experimentally studied. The first one is to...The principle of laser optical feedback microscope was presented and demonstrated. Three methods to advance the vertical resolution of laser optical feedback microscope were experimentally studied. The first one is to detect the two polarized lights’ intensities separately with a Wollaston prism instead of to detect the whole light’s intensity. The second is that both of the two orthogonally polarized lights of a birefringent dual frequency laser are fed back. The third one is that only one of the orthogonally polarized lights is fed back. The experimental results show that the modes competition between orthogonally polarized lights can be used to improve the vertical resolution of laser optical feedback microscope effectively.展开更多
A scanning near-field optical microscope using uncoated fiber tip is described, which can work in transmission and reflection configurations, both capable of working in illumination and collection-mode, so that either...A scanning near-field optical microscope using uncoated fiber tip is described, which can work in transmission and reflection configurations, both capable of working in illumination and collection-mode, so that either transparent or opaque sample can be investigated. Depending on different applications, either constant-gap or constant-height images can be achieved. A compact homemade translator permits to select interested area of sample in the range of 4 mm×4 mm. Working in the constant-height as well as transmission and collection-mode configuration, several kinds of samples such as holographic grating, liposome and zeolite were investigated. The experimental results revealed the dependence of the optical resolution of the SNOM on the tip-sample separation.展开更多
Over the past two decades, several fluorescence-and non-fluorescence-based optical microscopes have been developed to break the diffraction limited barrier. In this review, the basic principles implemented in microsco...Over the past two decades, several fluorescence-and non-fluorescence-based optical microscopes have been developed to break the diffraction limited barrier. In this review, the basic principles implemented in microscopy for super-resolution are described. Furthermore, achievements and instrumentation for super-resolution are presented. In addition to imaging, other applications that use super-resolution optical microscopes are discussed.展开更多
Stimulated emission depletion(STED) microscope is one of the most prominent super-resolution bio-imaging instruments, which holds great promise for ultrahigh-resolution imaging of cells. To construct a STED microscope...Stimulated emission depletion(STED) microscope is one of the most prominent super-resolution bio-imaging instruments, which holds great promise for ultrahigh-resolution imaging of cells. To construct a STED microscope, it is challenging to realize temporal synchronization between the excitation pulses and the depletion pulses. In this study, we present a simple and low-cost method to achieve pulse synchronization by using a condensed fluorescent dye as a depletion indicator. By using this method, almost all the confocal microscopes can be upgraded to a STED system without losing its original functions. After the pulse synchronization,our STED system achieved sub-100-nm resolution for fluorescent nanospheres and single-cell imaging.展开更多
Electron emission properties of single-walled carbon nanotubes (SWCNTs) assembled on a tungsten tip were investigated using field emission microscopy (FEM). The transmission electron microscopy (TEM) micrograph confir...Electron emission properties of single-walled carbon nanotubes (SWCNTs) assembled on a tungsten tip were investigated using field emission microscopy (FEM). The transmission electron microscopy (TEM) micrograph confirmed the existence of an SWCNT bundle on the W tip. Under appropriate experimental conditions,a series of FEM patterns with atomic resolution were obtained. These patterns arose possibly from the field emission of the open end of an individual (16,0) SWCNT protruding from the SWCNT bundle. The magnification factor and the resolution under our experimental conditions were calculated theoretically. If the value of the compression factor β was set at β= 1.76, the calculated value of the magnification factor was in agreement with the measured value. The resolving powerof FEM was determined by the resolution equation given by Gomer. The resolutionof 0.277 nm could be achieved under the typical electric field of 5.0×107 V/cm, which was close to the interatomic separation 0.246 nm between carbon atoms along the zigzag edge at the open end for the (16, 0) SWCNT. Consequently, our experimental results were further supported by our theoretical calculation.展开更多
Image restoration phase-filtering lateral superresolution confocal microscopy, a new approach, is proposed to achieve lateral superresolution using a confoeal microscope. This approach uses a lateral superresolution p...Image restoration phase-filtering lateral superresolution confocal microscopy, a new approach, is proposed to achieve lateral superresolution using a confoeal microscope. This approach uses a lateral superresolution pupil filter to preliminarily improve its lateral resolution and uses a siagle-image superresolution restoration technique based on a maximum likelihood estimate to further improve its lateral resolution. The new approach has the advantages of a low cost and the remarkable superresolution effect without excessive system complexity. Experiments indicate that the proposed approach can improve the lateral resolution of a confocal microscope from 0.3μm to less than 0.1 μm when λ = 632.8 nm and NA=0.85.展开更多
Franckeite and cylindrite of franckite mineral group were examined in the transmission electron microscope. Selected-area electron diffraction indicated that in the structure exists a long period, belonging to an inco...Franckeite and cylindrite of franckite mineral group were examined in the transmission electron microscope. Selected-area electron diffraction indicated that in the structure exists a long period, belonging to an incommensurate modulation period. The incommensurate modulation in this kind of minerals results from sine undular structure sheets. The excellent correspondence between high resolution simulated computer image and observed image was obtained on the basis of the structure of franckeite and cylindrite which was put forward in this paper. First, the fact that in the structures of franckeite and cylindrite exist two kinds of structure sheets whose lattices are different has been proved by experiment, i. e. false tetragonal symmetrical galena structure sheets（t sheets）and false hexagonal SnS<sub>2</sub> sheets（h sheets）. The two kinds of sheets are packed in order of htht…展开更多
We have built an integrated imaging system by combining stimulated emission depletion(STED)microscope and atomic force microscope(AFM).The STED microscope was constructed based on the supercontinuum fiber laser and a ...We have built an integrated imaging system by combining stimulated emission depletion(STED)microscope and atomic force microscope(AFM).The STED microscope was constructed based on the supercontinuum fiber laser and a super lateral resolution of42 nm was achieved.With this integrated imaging system,morphological features,mechanical parameters and fluorescence super resolution imaging were obtained simultaneously for both nanobeads and fixed cell samples.This new integrated imaging system is expected to obtain comprehensive information at the nanoscale for studies in nanobiology and nanomedicine.展开更多
基金The project supported by the National Natural Science Foundation of China
文摘Based on geometric moire method, moire interferometry and microscopic moire interferometry, a high spatial resolution and high sensitivity geometric microscopic moire method is presented. Geometric micron-moire patterns are produced by the superposition of two high frequency gratings through a microscope system. Compared with other grating-based photo-mechanics methods, microscopic moire method could provide whole-field moire patterns of both high spatial resolution and high sensitivity. The frequency of specimen and reference gratings used in this method can be from 1 line/mm to 10000 lines/mm. Additionally, a 4F optical filter system is used to enhance the contrast of microscopic moire patterns effectively.
基金Supported by Postgraduate Innovation Funding Project of Hebei Province(CXZZSS2019050)the Qinhuangdao City Key Research and Development Program Science and Technology Support Project(201801B010)
文摘An error correction technique for the micro-scanning instrument of the optical micro-scanning thermal microscope imaging system is proposed. The technique is based on micro-scanning technology combined with the proposed second-order oversampling reconstruction algorithm and local gradient image reconstruction algorithm. In this paper, we describe the local gradient image reconstruction model, the error correction technique, down-sampling model and the error correction principle. In this paper, we use a Lena original image and four low-resolution images obtained from the standard half-pixel displacement to simulate and verify the effectiveness of the proposed technique. In order to verify the effectiveness of the proposed technique, two groups of low-resolution thermal microscope images are collected by the actual thermal microscope imaging system for experimental study. Simulations and experiments show that the proposed technique can reduce the optical micro-scanning errors, improve the imaging effect of the system and improve the system's spatial resolution. It can be applied to other electro-optical imaging systems to improve their resolution.
文摘The principle of laser optical feedback microscope was presented and demonstrated. Three methods to advance the vertical resolution of laser optical feedback microscope were experimentally studied. The first one is to detect the two polarized lights’ intensities separately with a Wollaston prism instead of to detect the whole light’s intensity. The second is that both of the two orthogonally polarized lights of a birefringent dual frequency laser are fed back. The third one is that only one of the orthogonally polarized lights is fed back. The experimental results show that the modes competition between orthogonally polarized lights can be used to improve the vertical resolution of laser optical feedback microscope effectively.
文摘A scanning near-field optical microscope using uncoated fiber tip is described, which can work in transmission and reflection configurations, both capable of working in illumination and collection-mode, so that either transparent or opaque sample can be investigated. Depending on different applications, either constant-gap or constant-height images can be achieved. A compact homemade translator permits to select interested area of sample in the range of 4 mm×4 mm. Working in the constant-height as well as transmission and collection-mode configuration, several kinds of samples such as holographic grating, liposome and zeolite were investigated. The experimental results revealed the dependence of the optical resolution of the SNOM on the tip-sample separation.
基金Project supported by the Australian Research Council (ARC) through the Discovery Project (No.DP170101775)
文摘Over the past two decades, several fluorescence-and non-fluorescence-based optical microscopes have been developed to break the diffraction limited barrier. In this review, the basic principles implemented in microscopy for super-resolution are described. Furthermore, achievements and instrumentation for super-resolution are presented. In addition to imaging, other applications that use super-resolution optical microscopes are discussed.
基金supported by the National Natural Science Foundation of China (21227804, 21390414, 61378062, 21505148)National Key Research and Development Program (2016YFA0400902)the Natural Science Foundation of Shanghai (15ZR1448400, 14ZR1448000)
文摘Stimulated emission depletion(STED) microscope is one of the most prominent super-resolution bio-imaging instruments, which holds great promise for ultrahigh-resolution imaging of cells. To construct a STED microscope, it is challenging to realize temporal synchronization between the excitation pulses and the depletion pulses. In this study, we present a simple and low-cost method to achieve pulse synchronization by using a condensed fluorescent dye as a depletion indicator. By using this method, almost all the confocal microscopes can be upgraded to a STED system without losing its original functions. After the pulse synchronization,our STED system achieved sub-100-nm resolution for fluorescent nanospheres and single-cell imaging.
基金the National Natural Science Foundation of China(Grant Nos.69890221 and 69971003)the MOST of China(No.2001CB610503)+1 种基金Key Foundation for Science and Technology Research of Education Ministry of China(No.00005)Scientific Research Foundation for Returned Oversea Chinese Scholars,State Education Commission
文摘Electron emission properties of single-walled carbon nanotubes (SWCNTs) assembled on a tungsten tip were investigated using field emission microscopy (FEM). The transmission electron microscopy (TEM) micrograph confirmed the existence of an SWCNT bundle on the W tip. Under appropriate experimental conditions,a series of FEM patterns with atomic resolution were obtained. These patterns arose possibly from the field emission of the open end of an individual (16,0) SWCNT protruding from the SWCNT bundle. The magnification factor and the resolution under our experimental conditions were calculated theoretically. If the value of the compression factor β was set at β= 1.76, the calculated value of the magnification factor was in agreement with the measured value. The resolving powerof FEM was determined by the resolution equation given by Gomer. The resolutionof 0.277 nm could be achieved under the typical electric field of 5.0×107 V/cm, which was close to the interatomic separation 0.246 nm between carbon atoms along the zigzag edge at the open end for the (16, 0) SWCNT. Consequently, our experimental results were further supported by our theoretical calculation.
基金Supported by the National Natural Science Foundation of China under Grant No 50475035, and the Research Fund for the Doctoral Programme of Higher Education of China under Grant No 20050213035.
文摘Image restoration phase-filtering lateral superresolution confocal microscopy, a new approach, is proposed to achieve lateral superresolution using a confoeal microscope. This approach uses a lateral superresolution pupil filter to preliminarily improve its lateral resolution and uses a siagle-image superresolution restoration technique based on a maximum likelihood estimate to further improve its lateral resolution. The new approach has the advantages of a low cost and the remarkable superresolution effect without excessive system complexity. Experiments indicate that the proposed approach can improve the lateral resolution of a confocal microscope from 0.3μm to less than 0.1 μm when λ = 632.8 nm and NA=0.85.
基金Project supported by the National Natural Science Foundation of China.
文摘Franckeite and cylindrite of franckite mineral group were examined in the transmission electron microscope. Selected-area electron diffraction indicated that in the structure exists a long period, belonging to an incommensurate modulation period. The incommensurate modulation in this kind of minerals results from sine undular structure sheets. The excellent correspondence between high resolution simulated computer image and observed image was obtained on the basis of the structure of franckeite and cylindrite which was put forward in this paper. First, the fact that in the structures of franckeite and cylindrite exist two kinds of structure sheets whose lattices are different has been proved by experiment, i. e. false tetragonal symmetrical galena structure sheets（t sheets）and false hexagonal SnS<sub>2</sub> sheets（h sheets）. The two kinds of sheets are packed in order of htht…
基金supported by the National Basic Research Program of China(2013CB933701)the National Natural Science Foundation of China(91213305,21127901,21121063)Chinese Academy of Sciences
文摘We have built an integrated imaging system by combining stimulated emission depletion(STED)microscope and atomic force microscope(AFM).The STED microscope was constructed based on the supercontinuum fiber laser and a super lateral resolution of42 nm was achieved.With this integrated imaging system,morphological features,mechanical parameters and fluorescence super resolution imaging were obtained simultaneously for both nanobeads and fixed cell samples.This new integrated imaging system is expected to obtain comprehensive information at the nanoscale for studies in nanobiology and nanomedicine.
基金This project is supported by the Natural Science Foundation of China （NSFC）, the Chinese National ＂973＂ project of the Ministry of Science and Technology （MOST）, the Chinese Academy of Sciences and the Shanghai Supercomputer Center, H. T. acknowledges the ＂Centre de Calcul en Midi-Pyrenees＂ （CALMIP） for computational resources. H. T. also thanks Sebastien Gauthier for useful discussions.
