AIM: To identify the two polymorphisms of microsomal triglyceride transfer protein (MTP) gene in the Chinese population and to explore their correlation with both hepatitis B virus (HBV) self-limited infection and per...AIM: To identify the two polymorphisms of microsomal triglyceride transfer protein (MTP) gene in the Chinese population and to explore their correlation with both hepatitis B virus (HBV) self-limited infection and persistent infection. METHODS: A total of 316 subjects with self-limited HBV infection and 316 patients with persistent HBV infection (195 subjects without familial history), matched with age and sex, from the Chinese Han population were enrolled in this study. Polymorphisms of MTP at the promoter region -493 and at H297Q were determined by the allele specific polymerase chain reaction (PCR). RESULTS: The ratio of males to females was 2.13:1 for each group and the average age in the self-limited and chronic infection groups was 38.36 and 38.28 years, respectively. None of the allelic distributions deviated significantly from that predicted by the Hardy-Weinberg equilibrium. There was a linkagedisequilibrium between H297Q and -493G/T (D’ = 0.77). As the χ2 test was used, the genotype distribution of MTP -493G/T demonstrated a significant difference between the self-limited infection group and the entire chronic group or the chronic patients with no family history (χ2 = 8.543, P = 0.015 and χ2 = 7.199, P = 0.019). The allele distribution at the MTP-493 position also demonstrated a significant difference between the study groups without family history (χ2 = 6.212, P = 0.013). The T allele emerged as a possible protective factor which may influence the outcomes of HBV infection (OR: 0.59; 95% CI: 0.389-0.897). CONCLUSION: The polymorphism of the MTP gene, T allele at -493, may be involved in determining the HBV infection outcomes, of which the mechanism needs to be further investigated.展开更多
To explore the relationship between microsomal triglyceride transfer protein (MT P) gene variation and diabetic dyslipidemia among Chinese Methods Using PCR restriction fragment length polymorphism (PCR RFLP) anal...To explore the relationship between microsomal triglyceride transfer protein (MT P) gene variation and diabetic dyslipidemia among Chinese Methods Using PCR restriction fragment length polymorphism (PCR RFLP) analysis and gene sequencing, we studied the influence of a common MTP gene polymorphism in the p romoter region on the apoB containing lipoproteins in 44 Chinese type 2 diabeti c subjects and 32 non diabetic volunteers Results A common functional G/T polymorphism in 493 bp upstream from the transcriptional start point was detected among native Chinese There were 41 carriers (53 9%) of the MTP 493 G/G genotype, 28 (36 8%) of the MTP 493 G/T genotype and 7 (9 3%) of the MTP 493 T/T genotype The allele frequency of M TP 493 T in the diabetic group was 0 30 The MTP 493 T/T diabetic group had significantly higher TG ( P <0 05), VLDL CH ( P <0 05) and smaller LDL pa rticle size ( P <0 001) than the MTP 493 common genotype group Conclusion Genetic variation in the MTP promoter is likely to be highly involved in the pro duction of dyslipidemia in type 2 diabetic subjects展开更多
This study was performed for investigation the relationship between variants of MTP gene polymorphism and the development of NAFLD in patients with and without MS.The study was included 174 NAFLD patients(106 with MS ...This study was performed for investigation the relationship between variants of MTP gene polymorphism and the development of NAFLD in patients with and without MS.The study was included 174 NAFLD patients(106 with MS and 68 without MS),and 141 healthy control subjects.The 493 G/T polymorphism of MTP gene was evaluated by PCR-RFLP method.The frequency of MTP TT genotype and T allele were significantly higher in NAFLD patients when compared to healthy controls.Moreover,a significant association in MTP gene polymorphism was observed in NAFLD patients with MS compared to NAFLD patients without MS and controls.Our study suggested that MTP 493 G/T gene polymorphism may act as susceptibility biomarker for NAFLD and MS.展开更多
基金F.Hoffmann-La Roche Ltd Switzerland and the National High Technology ResearchDevelopment Program of China (863 Program), No. 2006AA02A411
文摘AIM: To identify the two polymorphisms of microsomal triglyceride transfer protein (MTP) gene in the Chinese population and to explore their correlation with both hepatitis B virus (HBV) self-limited infection and persistent infection. METHODS: A total of 316 subjects with self-limited HBV infection and 316 patients with persistent HBV infection (195 subjects without familial history), matched with age and sex, from the Chinese Han population were enrolled in this study. Polymorphisms of MTP at the promoter region -493 and at H297Q were determined by the allele specific polymerase chain reaction (PCR). RESULTS: The ratio of males to females was 2.13:1 for each group and the average age in the self-limited and chronic infection groups was 38.36 and 38.28 years, respectively. None of the allelic distributions deviated significantly from that predicted by the Hardy-Weinberg equilibrium. There was a linkagedisequilibrium between H297Q and -493G/T (D’ = 0.77). As the χ2 test was used, the genotype distribution of MTP -493G/T demonstrated a significant difference between the self-limited infection group and the entire chronic group or the chronic patients with no family history (χ2 = 8.543, P = 0.015 and χ2 = 7.199, P = 0.019). The allele distribution at the MTP-493 position also demonstrated a significant difference between the study groups without family history (χ2 = 6.212, P = 0.013). The T allele emerged as a possible protective factor which may influence the outcomes of HBV infection (OR: 0.59; 95% CI: 0.389-0.897). CONCLUSION: The polymorphism of the MTP gene, T allele at -493, may be involved in determining the HBV infection outcomes, of which the mechanism needs to be further investigated.
文摘To explore the relationship between microsomal triglyceride transfer protein (MT P) gene variation and diabetic dyslipidemia among Chinese Methods Using PCR restriction fragment length polymorphism (PCR RFLP) analysis and gene sequencing, we studied the influence of a common MTP gene polymorphism in the p romoter region on the apoB containing lipoproteins in 44 Chinese type 2 diabeti c subjects and 32 non diabetic volunteers Results A common functional G/T polymorphism in 493 bp upstream from the transcriptional start point was detected among native Chinese There were 41 carriers (53 9%) of the MTP 493 G/G genotype, 28 (36 8%) of the MTP 493 G/T genotype and 7 (9 3%) of the MTP 493 T/T genotype The allele frequency of M TP 493 T in the diabetic group was 0 30 The MTP 493 T/T diabetic group had significantly higher TG ( P <0 05), VLDL CH ( P <0 05) and smaller LDL pa rticle size ( P <0 001) than the MTP 493 common genotype group Conclusion Genetic variation in the MTP promoter is likely to be highly involved in the pro duction of dyslipidemia in type 2 diabetic subjects
基金This work was supported by grant from National Research Centre,Egypt(Project Number 10010205).
文摘This study was performed for investigation the relationship between variants of MTP gene polymorphism and the development of NAFLD in patients with and without MS.The study was included 174 NAFLD patients(106 with MS and 68 without MS),and 141 healthy control subjects.The 493 G/T polymorphism of MTP gene was evaluated by PCR-RFLP method.The frequency of MTP TT genotype and T allele were significantly higher in NAFLD patients when compared to healthy controls.Moreover,a significant association in MTP gene polymorphism was observed in NAFLD patients with MS compared to NAFLD patients without MS and controls.Our study suggested that MTP 493 G/T gene polymorphism may act as susceptibility biomarker for NAFLD and MS.
