Mikania cordata, an invasive weed is becom- ing a problem in the secondary degraded forests and plantations of Bangladesh, not only competing with but also releasing allelochemicals to cultivated crops. Allelo- pathic...Mikania cordata, an invasive weed is becom- ing a problem in the secondary degraded forests and plantations of Bangladesh, not only competing with but also releasing allelochemicals to cultivated crops. Allelo- pathic effects of the leaves of M. cordata were investigated through an experiment in the green house of the Institute of Forestry and Environmental Sciences, University of Chit- tagong, Bangladesh. Five economically important agricul- tural crops （Oryza sativa L., Triticum aestivum L., Vigna sinensis L., Abelmoschus esculentus L. and Amaranthus tricolor L.） and three forest crops （Acacia auriculiformis A. Cunn. ex Benth. ＆ Hook, Albizia procera （Roxb.） Benth., and Paraserianthes falcataria （L.） Nielson） were used as bioassay species. Experiments were conducted on trays at an average room temperature 27 ~C. The effects of dif- ferent concentrations of leaf extracts were compared to controls. Water soluble allelochemicals of M. cordata inhibited the germination and initial growth of the tested agricultural and forest crops. Germination percent of forest crops declined with increasing concentration of extracts. The inhibitory effect on root elongation of forest crops was pronounced, whereas a stimulatory effect was found on P. falcataria up to a concentration of 25 %. Abelmoschus esculentus showed poor germination （%） and low shoot development （0.5 cm） and no development of taproot or lateral roots at treatment of 100 % concentration. Theinhibitory effect was proportional to the concentrations of the extracts with higher concentrations producing stronger inhibitory effects. Lower concentrations showed some stimulatory effect. The inhibitory effect was pronounced in root and lateral root development rather than germination or shoot development of the crops. Mikania cordata should be eradicated for healthy growth and development of the forest and adjacent agricultural crops.展开更多
OBJECTIVE To isolate and characterize bioactive compound from traditionally important medicinal plant Mikania cordata,and to investigate muti-faced pharmacological activities of the isolated compound.METHODS A triterp...OBJECTIVE To isolate and characterize bioactive compound from traditionally important medicinal plant Mikania cordata,and to investigate muti-faced pharmacological activities of the isolated compound.METHODS A triterpeenoid,16-hydroxy betulinic acid(16 HBA) was isolated from Mikania cordata leaf and the structure of the compound was determined by NMR spectroscopic means.Antimicrobial activity of 16 HBA was tested by disc diffusion method and minimum inhibitory concentration(MIC) against the tested microorganisms was determined.The analgesic property of 16 HBA was tested using acetic acid-induced writhes in mice and hot plate thermal stimulation in rats.The anti-inflammatory activity was studied using carrageenin-induced paw edema method.The antipyretic potential of 16 HBA was evaluated by using yeast-induced hyperthermia in rats.RESULTS The triterpenoid 16 HBA showed potent antibacterial activity with inhibition zone of diameter ranging from 12.0~17.5 mm and antifungal activity with mycelial growth inhibition ranges from 37.6%~54.5%.The MIC values for antibacterial and antifungal activities ranged from 31.5~125 and 250~1000 μg·mL-1 respectively.The compound(50 and100 mg·kg^(-1) body weight) showed potent peripheral and central analgesic activity having 55.19% and41% of writhing inhibition at 90 min after administration of the compound and the highest 55.98%,79.18% elongation of reaction time,respectively.In anti-inflammatory activity screening,the compound(100 mg·kg^(-1)) revealed the highest 77.08% edema inhibition at 4 h after administration of carrageenan.In antipyretic assay,16 HBA exhibited a strong antipyretic effect in yeast-induced rats.CONCLUSION The present study confirmed that 16-hydroxy betulinic acid isolated from Mikania cordata has potent antimicrobial,analgesic,anti-inflammatory and antipyretic properties.Our results can be seen as scientific support for the traditional and folklore usage of Mikania cordata in Bangladesh for the treatment of different ailments and provide opportunities to explore this plant as a source bioactive compounds for biochemical and pharmaceutical industries.展开更多
文摘Mikania cordata, an invasive weed is becom- ing a problem in the secondary degraded forests and plantations of Bangladesh, not only competing with but also releasing allelochemicals to cultivated crops. Allelo- pathic effects of the leaves of M. cordata were investigated through an experiment in the green house of the Institute of Forestry and Environmental Sciences, University of Chit- tagong, Bangladesh. Five economically important agricul- tural crops （Oryza sativa L., Triticum aestivum L., Vigna sinensis L., Abelmoschus esculentus L. and Amaranthus tricolor L.） and three forest crops （Acacia auriculiformis A. Cunn. ex Benth. ＆ Hook, Albizia procera （Roxb.） Benth., and Paraserianthes falcataria （L.） Nielson） were used as bioassay species. Experiments were conducted on trays at an average room temperature 27 ~C. The effects of dif- ferent concentrations of leaf extracts were compared to controls. Water soluble allelochemicals of M. cordata inhibited the germination and initial growth of the tested agricultural and forest crops. Germination percent of forest crops declined with increasing concentration of extracts. The inhibitory effect on root elongation of forest crops was pronounced, whereas a stimulatory effect was found on P. falcataria up to a concentration of 25 %. Abelmoschus esculentus showed poor germination （%） and low shoot development （0.5 cm） and no development of taproot or lateral roots at treatment of 100 % concentration. Theinhibitory effect was proportional to the concentrations of the extracts with higher concentrations producing stronger inhibitory effects. Lower concentrations showed some stimulatory effect. The inhibitory effect was pronounced in root and lateral root development rather than germination or shoot development of the crops. Mikania cordata should be eradicated for healthy growth and development of the forest and adjacent agricultural crops.
基金supported by Centre for Biomedical Research,University of Dhaka and Department of Applied Chemistry＆Chemical Technology,Islamic University,Kushtia,Bangladesh
文摘OBJECTIVE To isolate and characterize bioactive compound from traditionally important medicinal plant Mikania cordata,and to investigate muti-faced pharmacological activities of the isolated compound.METHODS A triterpeenoid,16-hydroxy betulinic acid(16 HBA) was isolated from Mikania cordata leaf and the structure of the compound was determined by NMR spectroscopic means.Antimicrobial activity of 16 HBA was tested by disc diffusion method and minimum inhibitory concentration(MIC) against the tested microorganisms was determined.The analgesic property of 16 HBA was tested using acetic acid-induced writhes in mice and hot plate thermal stimulation in rats.The anti-inflammatory activity was studied using carrageenin-induced paw edema method.The antipyretic potential of 16 HBA was evaluated by using yeast-induced hyperthermia in rats.RESULTS The triterpenoid 16 HBA showed potent antibacterial activity with inhibition zone of diameter ranging from 12.0~17.5 mm and antifungal activity with mycelial growth inhibition ranges from 37.6%~54.5%.The MIC values for antibacterial and antifungal activities ranged from 31.5~125 and 250~1000 μg·mL-1 respectively.The compound(50 and100 mg·kg^(-1) body weight) showed potent peripheral and central analgesic activity having 55.19% and41% of writhing inhibition at 90 min after administration of the compound and the highest 55.98%,79.18% elongation of reaction time,respectively.In anti-inflammatory activity screening,the compound(100 mg·kg^(-1)) revealed the highest 77.08% edema inhibition at 4 h after administration of carrageenan.In antipyretic assay,16 HBA exhibited a strong antipyretic effect in yeast-induced rats.CONCLUSION The present study confirmed that 16-hydroxy betulinic acid isolated from Mikania cordata has potent antimicrobial,analgesic,anti-inflammatory and antipyretic properties.Our results can be seen as scientific support for the traditional and folklore usage of Mikania cordata in Bangladesh for the treatment of different ailments and provide opportunities to explore this plant as a source bioactive compounds for biochemical and pharmaceutical industries.
