Our previous studies demonstrated that the region around markers BMS470 and BMS1242 on BTA6 showed a linkage to 305-d milk yield and composition traits in the Chinese Holstein population. We herein focused on such nar...Our previous studies demonstrated that the region around markers BMS470 and BMS1242 on BTA6 showed a linkage to 305-d milk yield and composition traits in the Chinese Holstein population. We herein focused on such narrow region to fine map milk production QTLs with 15 SNPs across 25 Mb with each SNP in 1 Mb within most regions in a Chinese Holstein population with daughter design. 1 449 Holstein cows and 11 sires were genotyped for such SNPs by using TaqMan probe and RFLP assays. Multipoint linkage analysis across family revealed a QTL affecting milk yield between PPARGC1A C4075T and SLC34A2 T1713C. Meanwhile, within family analysis found three milk yield QTLs (two in CR T60984131G-CEP135 C501T and one in PDLIM5 A106C-OPN T3907, a fat yield QTLin UGDH T1670C-CR T60984131G region, and two protein yield QTLs in TBC1D1 G501C-UGDH T1670C and PPARGC1A C4075T-SLC34A2 T1713C, respectively. Associations between aforementioned significant SNP markers and milk production traits were further implemented. We found significant associations of PPARGC1A C4075T, SLC34A2 T1713C with milk yield (P0.05, P0.01, P0.01), UGDH T1670C, and CR T60984131G with fat yield (P0.01, P0.01), and PPARGC1A C4075T, SLC34A2 T1713C, UGDH T1670C and OPN T3907 with protein yield (P0.01, P0.01, P0.01, P0.01). Our findings implied that QTLs affecting milk production traits on BTA6 were pleictropism or multigenic effect and PPARGC1A and OPN may be the causal mutations behind milk production QTLs on BTA6 in the Chinese Holstein population.展开更多
Background:Phosphorus(P) supplementation is costly and can result in excess P excretion.This study investigated the effects of reducing dietary P on milk production and P excretion in dairy cows over a full lactati...Background:Phosphorus(P) supplementation is costly and can result in excess P excretion.This study investigated the effects of reducing dietary P on milk production and P excretion in dairy cows over a full lactation.Method:Forty-five multiparous Holstein dairy cows were divided into 15 blocks according to expected calving date and previous milk yield,and assigned randomly to one of the three dietary treatments:0.37,0.47,and 0.57%P(DM basis);these P levels represent the NRC recommendations,Chinese recommendations,and the amount of dietary P commonly fed by Chinese dairy farmers,respectively.Average daily feed intake was calculated from monthly data on feed offered and refused.Milk yields of individual cows were recorded weekly,and milk samples were taken for analysis of protein,fat,solids-not-fat,lactose,and somatic cell count.Blood samples were collected on days-6,-3,0,3,6 relative to calving,and then monthly throughout lactation,and analyzed for P and Ca concentrations.Spot samples of feces and urine were collected for 3 consecutive d during weeks 12,24,and 36,and P concentrations were analyzed.Reproduction and health data were recorded.Results:Dietary P did not affect dry matter intake or milk yield(P〉 0.10).Milk fat content was slightly higher in cows fed 0.37%P than in cows fed 0.47%P(P = 0.05).Serum concentrations of P and Ca did not reflect dietary P content(P〉 0.10).Fecal and urinary P both declined linearly(P〈 0.05) as dietary P decreased from 0.57 to0.37%.Fecal P content was 25%less when dietary P was 0.37%compared to 0.57%.Health events and reproductive performance were not associated with dietary P content(P〉 0.05).Conclusions:Lowering dietary P from 0.57 to 0.37%did not negatively affect milk production,but did significantly reduce P excretion into environment.展开更多
Background:Economically important milk production traits including milk volume,milk fat and protein yield vary considerably across dairy goats in New Zealand.A significant portion of the variation is attributable to g...Background:Economically important milk production traits including milk volume,milk fat and protein yield vary considerably across dairy goats in New Zealand.A significant portion of the variation is attributable to genetic variation.Discovery of genetic markers linked to milk production traits can be utilised to drive selection of highperformance animals.A previously reported genome wide association study across dairy goats in New Zealand identified a quantitative trait locus(QTL)located on chromosome 19.The most significantly associated single nucleotide polymorphism(SNP)marker for this locus is located at position 26,610,610(SNP marker rs268292132).This locus is associated with multiple milk production traits including fat,protein and volume.The predicted effect of selection for the beneficial haplotype would result in an average production increase of 2.2 kg fat,1.9 kg protein and 73.6 kg milk yield.An outstanding question was whether selection for the beneficial allele would co-select for any negative pleiotropic effects.An adverse relationship between milk production and udder health traits has been reported at this locus.Therefore,a genome wide association study was undertaken looking for loci associated with udder traits.Results:The QTL and production associated marker rs268292132 was identified in this study to also be associated with several goat udder traits including udder depth(UD),fore udder attachment(FUA)and rear udder attachment(RUA).Our study replicates the negative relationship between production and udder traits with the high production allele at position 19:26,610,610(SNP marker rs268292132)associated with an adverse change in UD,FUA and RUA.Conclusions:Our study has confirmed the negative relationship between udder traits and production traits in the NZ goat population.We have found that the frequency of the high production allele is relatively high in the NZ goat population,indicating that its effect on udder conformation is not significantly detrimental on animal health.It will however be important to monitor udder conformation as the chromosome 19 locus is progressively implemented for marker assisted selection.It will also be of interest to determine if the gene underlying the production QTL has a direct effect on mammary gland morphology or whether the changes observed are a consequence of the increased milk volume.展开更多
Goats are one of the oldest domesticated animal species widely distributed in the world playing an important role in the food production system in Sub-Saharan African Region (SSAR). Due to their multiple uses (milk pr...Goats are one of the oldest domesticated animal species widely distributed in the world playing an important role in the food production system in Sub-Saharan African Region (SSAR). Due to their multiple uses (milk production, meat, fiber and hides) and adaptation aptitudes to ecological conditions, goats produce and contribute positively to farmers’ socio-economy status in various production systems. This review aimed at giving a summary overview on the goat’s production systems characteristics, the genetic diversity and the candidate genes affecting reproductive and milk production performances in goat breeds in SSAR. It has been observed that traditional livestock production system with communal grazing system is the most used in goat keeping in SSAR. The geographical locations play an important role in the relationships between goat’s distributions in the region. At the same time, goats might have been differentiated and isolated one to others due to the wide geographic range, the diversify climate and the topography in the region. Among the six worldwide known haplogroups of goat (A, B, C, D, G and F), haplogroup A is the most representative in SSAR. However, haplogroup G and B can be found in some goat populations in some countries in east (Kenya and Ethiopia) and south parts of Africa. This review reveals that little is known on the candidate genes associated with prolificacy and milk production traits in indigenous goat breeds in the region. That observation suggests the importance of assessing candidate genes associated with economic traits in the populations of goat in SSAR.展开更多
This paper describes milk production and livestock production in Tibet. Some information of market demand has also been presented. There has been very little information published in Tibetan journals on production and...This paper describes milk production and livestock production in Tibet. Some information of market demand has also been presented. There has been very little information published in Tibetan journals on production and nutrition of cattle. This review provides a brief introduction to feeding systems and feeding resources. Many studies on milk production have been done in isolation, and do not go beyond the basic and practical level. Compared with dairy cattle research in other parts of China, large gaps in knowledge still exist in cattle production science, particularly related to nutrition, and systems approaches for the development of a dairy industry.展开更多
To fine map the previously detected quantitative trait loci (QTLs) affecting milk production traits on bovine chromosome 6 (BTA6), 15 microsatellite markers situated within an interval of 14.3 cM spanning from BMS...To fine map the previously detected quantitative trait loci (QTLs) affecting milk production traits on bovine chromosome 6 (BTA6), 15 microsatellite markers situated within an interval of 14.3 cM spanning from BMS690 to BM4528 were selected and 918 daughters of 8 sires were genotyped. Two mapping approaches, haplotype sharing based LD mapping and single marker regression mapping, were used to analyze the data. Both approaches revealed a quantitative trait locus (QTL) with significant effects on milk yield, fat yield and protein yield located in the segment flanked by markers BMS483 and MNB209, which spans a genetic distance of 0.6 cM and a physical distance of 1.5 Mb. In addition, the single marker regression mapping also revealed a QTL affecting fat percentage and protein percentage at marker DIK2291. Our fine mapping work will facilitate the cloning of candidate genes underlying the QTLs for milk production traits.展开更多
Thirty lactating Holstein cows were used to investigate the effects of different forages quality on milk fatty acids(FA) profiles and production. The cows were assigned to 3 dietary treatments(n = 10 per treatment) in...Thirty lactating Holstein cows were used to investigate the effects of different forages quality on milk fatty acids(FA) profiles and production. The cows were assigned to 3 dietary treatments(n = 10 per treatment) in a randomized block design with 3 repeated measures. They were fed the experimental diets for 90 d with 3 days of collection of samples for analysis at about 27 d intervals(samples were collected on days 28,29,30, 58, 59, 60.88, 89 and 90). The treatments were(DM basis): 1) mixed forages diet(MF) consisting of 3.7% Chinese wild rye, 26.7% corn silage and 23.4% alfalfa hay; 2) corn stalk diet 1(CS1) where corn stalk was used to formulate a similar chemical nutrient level to MF; 3) corn stalk diet 2(CS2) which used corn stalk to formulate a similar forage level to MF for the diet. Dry matter intake and BW were similar between treatments, but daily milk yield, milk fat and protein yield decreased(P < 0.05)in CS1 and CS2 compared with MF, with CS2 being the lowest(P < 0.05). In total FA of milk, the compositions of C18:1 c9, C18:3 and unsaturated FA increased(P < 0.05) in CS1 and CS2 compared with MF,and C18:0 and trans-C18:1 were trended to increase(P < 0.10), but C4:0-C16:0 were decreased(P < 0.05).Compared with cows fed CS2, cows receiving CS1 increased the compositions of C4:0 to C12:0 and C18:2(P < 0.05). The results suggests feeding corn stalk could produce a greater proportion of unsaturated fatty acid(UFA) in milk fat without resulting in milk fat depression(MFD) in mid lactation cows, but simply increasing the ratio of concentrate in low forages diets is not an effective way to increase milk fat synthesis and milk production.展开更多
Genome-wide association studies with an Illumina Bovine50K chip have detected 105 SNPs associated with one or multiple milk production traits in the Chinese Holstein population.Of these,38 significant SNPs detected wi...Genome-wide association studies with an Illumina Bovine50K chip have detected 105 SNPs associated with one or multiple milk production traits in the Chinese Holstein population.Of these,38 significant SNPs detected with high confidence by both L1-TDT and MMRA methods were selected to further mine potential key genes affecting milk yield and milk composition.By blasting the flanking sequences of these 38 SNPs with the bovine genome sequence combined with comparative genomics analysis,26 genes were found to contain or be near to such SNPs.Among them,the C14H8orf33 gene is merely 87 bp away from the significant SNP,Hapmap30383-BTC-005848.Hence,we report herein genotype-phenotype associations to further validate the genetic effects of the C14H8orf33 gene.By pooled DNA sequencing of 14 unrelated Holstein sires,a total of 18 with seven novel SNPs were identified.Among them,nine SNPs were in the 5′regulatory region,one in exon 6 and the other in the 3′UTR and 3′regulatory region.A total of nine of these identified SNPs were successfully genotyped and analyzed by mass spectrometry for association with five milk production traits in an independent resource population.The results showed that these SNPs were statistically significant for more than two traits[P<(0.0001–0.0267)].In addition,mRNA expression analyses revealed that C14H8orf33 was ubiquitous in eight different tissues,with a relatively higher expression level in the mammary gland than in other tissues.These findings,therefore,provide strong evidence for association of C14H8orf33 variants with milk yield and milk composition traits and may be applied in Chinese Holstein breeding programs.展开更多
The objective of this study was to analyze the effect of adding meteorological data to the training process of two milk production forecast models.The two models chosen were the nonlinear auto-regressive model with ex...The objective of this study was to analyze the effect of adding meteorological data to the training process of two milk production forecast models.The two models chosen were the nonlinear auto-regressive model with exogenous input(NARX)and the multiple linear regression(MLR)model.The accuracy of these models were assessed using seven different combinations of precipitation,sunshine hours and soil temperature as additional model training inputs.Lactation data(daily milk yield and days in milk)from 39 pasture-based Holstein-Friesian Irish dairy cows were selected to compare to the model outputs from a central database.The models were trained using historical milk production data from three lactation cycles and were employed to predict the total daily milk yield of a fourth lactation cycle for each individual cow over short(10-day),medium(30-day)and long-term(305-day)forecast horizons.The NARX model was found to provide a greater prediction accuracy when compared to the MLR model when predicting annual individual cow milk yield(kg),with R2 values greater than 0.7 for 95.5%and 14.7%of total predictions,respectively.The results showed that the introduction of sunshine hours,precipitation and soil temperature data improved the prediction accuracy of individual cow milk prediction for the NARX model in the short,medium and long-term forecast horizons.Sunshine hours was shown to have the largest impact on milk production with an improvement of forecast accuracy observed in 60%and 70%of all predictions(for all 39 test cows from both groups).However,the overall improvement in accuracy was small with a maximum forecast error reduction of 4.3%.Thus,the utilization of meteorological parameters in milk production forecasting did not have a substantial impact on forecast accuracy.展开更多
The study evaluated the effects of a diet incorporating a mango-based feed on the technical-economic performance of milk production of Montbeliard cows at the end of lactation in a dairy farm in Bobo-Dioulasso, Burkin...The study evaluated the effects of a diet incorporating a mango-based feed on the technical-economic performance of milk production of Montbeliard cows at the end of lactation in a dairy farm in Bobo-Dioulasso, Burkina Faso. To do so, 14 cows were subjected to 3 diets, including an experimental diet incorporating mango (ED) in comparison to a balanced control diet (BCD) and to the feeding practice of the farmer (FCD) during 60 days. Results show that the average amount of milk milked per day from ED (10.93 ± 0.17 L) was significantly higher (p < 0.05) than BCD (8.80 ± 0.19 L) and RTA (5.39 ± 0.14 L). The conversion index of FPD (4.24 ± 0.14) was significantly higher (p < 0.05) than that of BCD (1.75 ± 0.19) and ED (1.26 ± 0.16). Average feed costs per liter of milk (FC/L) were significantly different (p (125.95 ± 4.56 FCFA), BCD (83.67 ± 6.36 FCFA) and ED (60.06 ± 5.49 FCFA). The average gross profit margins (GPM) were significantly higher and decreasing (p ,953 ± 5031 FCFA), BCD (167,157 ± 5831 FCFA) and FCD (74,188 ± 4185 FCFA). Mango feed can be used in the diet of dairy cows. Its use contributes to the reduction of production costs and the generation of substantial profits. The valorization of mango residues is an alternative for a greater availability of feed at lower cost for the animals. It could contribute to a better management of processing residues that pollute the environment and to the reduction of fruit flies that affect orchard yields through reinfestation. The increased use of mango in dairy cattle feed should be encouraged.展开更多
Background Dietary fat is important for energy provision and immune function of lactating sows and their progeny.However,knowledge on the impact of fat on mammary transcription of lipogenic genes,de novo fat synthesis...Background Dietary fat is important for energy provision and immune function of lactating sows and their progeny.However,knowledge on the impact of fat on mammary transcription of lipogenic genes,de novo fat synthesis,and milk fatty acid(FA)output is sparse in sows.This study aimed to evaluate impacts of dietary fat levels and FA composition on these traits in sows.Forty second-parity sows(Danish Landrace×Yorkshire)were assigned to 1 of 5 dietary treatments from d 108 of gestation until weaning(d 28 of lactation):low-fat control diet(3%added animal fat);or 1 of 4 high-fat diets with 8%added fat:coconut oil(CO),fish oil(FO),sunflower oil(SO),or 4%octanoic acid plus 4%FO(OFO).Three approaches were taken to estimate de novo milk fat synthesis from glucose and body fat.Results Daily intake of FA was lowest in low-fat sows within fat levels(P<0.01)and in OFO and FO sows within highfat diets(P<0.01).Daily milk outputs of fat,FA,energy,and FA-derived carbon reflected to a large extent the intake of those.On average,estimates for de novo fat synthesis were 82 or 194 g/d from glucose according to method 1 or 2 and 255 g de novo+mobilized FA/d according to method 3.The low-fat diet increased mammary FAS expression(P<0.05)and de novo fat synthesis(method 1;P=0.13)within fat levels.The OFO diet increased de novo fat synthesis(method 1;P<0.05)and numerically upregulated mammary FAS expression compared to the other high-fat diets.Across diets,a daily intake of 440 g digestible FA minimized milk fat originating from glucose and mobilized body fat.Conclusions Sows fed diets with low-fat or octanoic acid,through upregulating FAS expression,increased mammary de novo fat synthesis whereas the milk FA output remained low in sows fed the low-fat diet or high-fat OFO or FO diets,indicating that dietary FA intake,dietary fat level,and body fat mobilization in concert determine de novo fat synthesis,amount and profiles of FA in milk.展开更多
Background: Identifying associations between genetic markers and traits of economic importance will provide practical benefits for the dairy goat industry, enabling genomic prediction of the breeding value of individu...Background: Identifying associations between genetic markers and traits of economic importance will provide practical benefits for the dairy goat industry, enabling genomic prediction of the breeding value of individuals, and facilitating discovery of the underlying genes and mutations. Genome-wide association studies were implemented to detect genetic regions that are significantly associated with effects on lactation yields of milk(MY), fat(FY),protein(PY) and somatic cell score(SCS) in New Zealand dairy goats.Methods: A total of 4,840 goats were genotyped with the Caprine 50 K SNP chip(Illumina Inc., San Diego, CA).After quality filtering, 3,732 animals and 41,989 SNPs were analysed assuming an additive linear model. Four GWAS models were performed, a single-SNP additive linear model and three multi-SNP Bayes C models. For the single-SNP GWAS, SNPs were fitted individually as fixed covariates, while the Bayes C models fit all SNPs simultaneously as random effects. A cluster of significant SNPs were used to define a haplotype block whose alleles were fitted as covariates in a Bayesian model. The corresponding diplotypes of the haplotype block were then fit as class variables in another Bayesian model.Results: Across all four traits, a total of 43 genome-wide significant SNPs were detected from the SNP GWAS. At a genome-wide significance level, the single-SNP analysis identified a cluster of variants on chromosome 19 associated with MY, FY, PY, and another cluster on chromosome 29 associated with SCS. Significant SNPs mapped in introns of candidate genes(45%), in intergenic regions(36%), were 0–5 kb upstream or downstream of the closest gene(14%) or were synonymous substitutions(5%). The most significant genomic window was located on chromosome 19 explaining up to 9.6% of the phenotypic variation for MY, 8.1% for FY, 9.1% for PY and 1% for SCS.Conclusions: The quantitative trait loci for yield traits on chromosome 19 confirms reported findings in other dairy goat populations. There is benefit to be gained from using these results for genomic selection to improve milk production in New Zealand dairy goats.展开更多
The main purpose of this study was to determine the effect of a corn straw or mixed diet on milk production, milk composition and the expression of genes associated with lactation in mid-lactation Chinese Holstein cow...The main purpose of this study was to determine the effect of a corn straw or mixed diet on milk production, milk composition and the expression of genes associated with lactation in mid-lactation Chinese Holstein cows. In this study, 10 healthy Chinese Holstein cows were randomly assigned to two groups and fed with different diets respectively, corn straw (CS) or mixed forage (MF) diet. CS group was fed roughage consisting of 53.8% corn straw only and the forge to concentrate (F : C) ratio [dry matter (DM)] was about 40: 60. MF group was fed roughage consisting of 3.7% Chinese wildrye and 23.4% alfalfa hay, the forge to concentrate (F : C) ratio (DM) was 70: 30. All the cows were fed 8 weeks and body weight, dry matter intake, body condition score, fat, protein, lactose, milk yield, total solid and somatic cell count (SCC) were recorded. Quantitative real-time PCR (qRT-PCR) was used to analyze cow mammary gland samples representing two different diets. The results suggested that different diet types had significant effects on milk yield, lactose, milk fat, milk protein, dry matter intake and somatic cell count in dairy cows, and cows fed MF diet improved milk production and lactation performance clearly (P〈0.05). In addition, mRNA expression of genes ACC, roTOR, STATS, CSN2, PPAR),, FABP3 and PTEN in MF group was extremely significantly higher than that in CS group (P〈0.05). mRNA expression ofAKT1, FAS, SCD and SREBPlc in MF group was significantly higher than that in CS group (P〈0.01). In summary, the milk yield and composition in mixed forage group were significantly improved than those in corn straw group.展开更多
[Objective] The paper was to increase utilization rate of roughage to solve current shortage of forage and improve production performance of Simmental cow.[Method] Fruit-flavored agent and green pigment were added in ...[Objective] The paper was to increase utilization rate of roughage to solve current shortage of forage and improve production performance of Simmental cow.[Method] Fruit-flavored agent and green pigment were added in roughage of Simmental cow respectively,and their effects on feed intake,milk production and milk quality of Simmental cow were studied.[Result] After adding fruit-flavored agent and green pigment,the feed intakes of Simmental cow were increased by 30.69% and 12.27%,while the milk productions were increased by 1.74 and 2.25 kg,respectively,and the differences were all significant(P 〈 0.05).[Conclusion]Adding 0.3% and 0.1% fruit-flavored agent and green pigment in roughage of Simmental cow could significantly improve feed intake and milk production,which can also delay the decline of milk production during late lactation stage and improve milk quality.展开更多
Objective To develop an analytical method for simultaneously qualitative and quantitative determination of melamine and triazine-related by-products including ammelide, ammeline, and cyanuric acid in milk and milk pro...Objective To develop an analytical method for simultaneously qualitative and quantitative determination of melamine and triazine-related by-products including ammelide, ammeline, and cyanuric acid in milk and milk products by gas chromatography- tandem mass spectrometry (GC-MS/MS). Methods Melamine and triazine-related by-products namely ammelide, ammeline and cyanuric acid in the samples were extracted in a solvent mixture of diethylamine, water, and acetonitrile (10:40:50, V/V/V). After centrifugation, an aliquot of the supernatant was evaporated to dryness under a gentle stream of nitrogen gas, and then melamine and triazine-related by-products were derivatized using BSTFA with 1% TMCS. The derivatives of melamine and its analogues were determined by gas chromatography/tandem mass spectrometry using multiple reactional monitoring (MRM) with 2, 6-Diamino-4-chloropyrimidine (DACP) being used as an internal standard. Results The linear detectable ranges were from 0.004 mg/kg to 1.6 mg/kg for melamine, ammelide, ammeline, and cyanuric acid with a correlation coefficient no less than 0.999. The recovery rates of the tour compounds in spiked blank milk powder at concentrations 0.5, 1, 2 mg/kg were between 61.4%-117.2%, and the relative standard deviation was no more than 11.5% (n=6). The detection limits of melamine, ammelide, ammeline and cyanuric acid in milk powder were 0.002 mg/kg with a ratio of signal to noise of 3. Conclusion This GC-MS/MS method for simultaneous determination of melamine, ammelide, ammeline, and cyanuric acid in milk and milk products is sensitive and specific.展开更多
Scientific and technical progress has been the driving forces of enterprises development.Milk productive enterprises are developing faster and growing better.It is very important to measure the contributive ratio of s...Scientific and technical progress has been the driving forces of enterprises development.Milk productive enterprises are developing faster and growing better.It is very important to measure the contributive ratio of scientific and technical progress in milk productive enterprises.And the appraisement could help to develop milk productive enterprises.The model C^2GS^2 was established to appraise the contributive ratio of scientific and technical progress in milk productive enterprises in the research.And the appraisement on the contributive ratio of scientific and technical progress in milk productive enterprises was made by the model.In the results of appraisement,science and technology play a main role in milk productive enterprises.It is shown that our milk productive enterprises are developed by scientific and technical progress while not by input of productive factors.展开更多
In this study,a label-free,portable and reproducible immunochip based on quartz crystal microbalance(QCM)was developed for the qualitative detection ofβ-lactoglobulin(β-LG),an allergen,in milk products.Experimental ...In this study,a label-free,portable and reproducible immunochip based on quartz crystal microbalance(QCM)was developed for the qualitative detection ofβ-lactoglobulin(β-LG),an allergen,in milk products.Experimental parameters in the fabrication and regeneration procedure such as pH of the coupling microenvironment,amount of anti-β-LG antibody and regeneration reagent were optimized in detail.Under optimal conditions,the proposed QCM immunochip exhibited good recognition of β-LG,with a calibration curve of ΔF=12.877 C_(β-LG)^(0.4809)(R^(2)=0.9982)and limit of detection of 0.04μg/mL.Additionally,this portable QCM immunochip had good stability,high specificity,and no obvious cross-reaction to three other milk proteins(α-casein,α-lactalbumin,and lactoferrin).It could compete a qualitative measurement within5 min,and could be reused at least ten times.In the β-LG analysis of actual milk samples,the developed QCM immunochip yielded reliable and accurate results,which correlated strongly with those from the standard HPLC method(R^(2)=0.9969).Thus,the portable,stable,and reproducible QCM immunochip developed in this study allowed the rapid,cost-effectively and sensitively measure theβ-LG in milk products.展开更多
<i>Aflatoxin</i> M1 (AFM1) occurrence in milk and milk products has been a major concern among food scientists over the past three decades owing to its possible health risk in humans. The well-documented A...<i>Aflatoxin</i> M1 (AFM1) occurrence in milk and milk products has been a major concern among food scientists over the past three decades owing to its possible health risk in humans. The well-documented AFM1 associated adverse health outcomes include hepatocarcinogens, mutagens genotoxins, mutagenicity, and teratogen. The paper reviews the occurrence of AFM1 in milk, milk products, and human milk in developing and developed countries, with its safety standards of regulation. The health associated risks with AFM1 are the important methods used in detecting and reducing AFM1 in milk and other milk products are presented.展开更多
Dairy products are nutritious food items that contain various essential nutrients,however,it has been proven that residual antibiotics have contaminated such products.These residues can cause several side effects on h...Dairy products are nutritious food items that contain various essential nutrients,however,it has been proven that residual antibiotics have contaminated such products.These residues can cause several side effects on human health.They increase antimicrobial resistance against several threatening microorganisms,as well as significant growth in allergenic reactions.Various methods,including heat treatments,have been applied to alleviate and reduce the effect of antibiotic residue level in milk and milk products.Changes in drug levels were not sig-nificantly remarkable,obliging researchers to find new approaches to prevent or reduce their risk and limit their complications on human health.展开更多
Milk and dairy products are more and more popular with consumers due to their various nutrients, and their quality and safety issues have always been concerned. Therefore, the development of rapid, accurate and simple...Milk and dairy products are more and more popular with consumers due to their various nutrients, and their quality and safety issues have always been concerned. Therefore, the development of rapid, accurate and simple screening techniques is of great significance. Liquid chromatography-high resolution time-of-flight mass spectrometry has high-resolution and high-throughput detection functions, and has gradually begun to be applied in the detection of milk and dairy products. This paper summarized the application of milk and dairy products in liquid chromatography-high resolution time-of-flight mass spectrometry, laying a foundation for the development of new methods.展开更多
基金supported by the National 948 Project of China (2006-G48)the National Key Technologies R&D Program of China (2006BAD04A01)+1 种基金the Key Development of New Transgenic Breeds Program of China (2009ZX08009-156B)the National Natural Science Foundation of China (31072016)
文摘Our previous studies demonstrated that the region around markers BMS470 and BMS1242 on BTA6 showed a linkage to 305-d milk yield and composition traits in the Chinese Holstein population. We herein focused on such narrow region to fine map milk production QTLs with 15 SNPs across 25 Mb with each SNP in 1 Mb within most regions in a Chinese Holstein population with daughter design. 1 449 Holstein cows and 11 sires were genotyped for such SNPs by using TaqMan probe and RFLP assays. Multipoint linkage analysis across family revealed a QTL affecting milk yield between PPARGC1A C4075T and SLC34A2 T1713C. Meanwhile, within family analysis found three milk yield QTLs (two in CR T60984131G-CEP135 C501T and one in PDLIM5 A106C-OPN T3907, a fat yield QTLin UGDH T1670C-CR T60984131G region, and two protein yield QTLs in TBC1D1 G501C-UGDH T1670C and PPARGC1A C4075T-SLC34A2 T1713C, respectively. Associations between aforementioned significant SNP markers and milk production traits were further implemented. We found significant associations of PPARGC1A C4075T, SLC34A2 T1713C with milk yield (P0.05, P0.01, P0.01), UGDH T1670C, and CR T60984131G with fat yield (P0.01, P0.01), and PPARGC1A C4075T, SLC34A2 T1713C, UGDH T1670C and OPN T3907 with protein yield (P0.01, P0.01, P0.01, P0.01). Our findings implied that QTLs affecting milk production traits on BTA6 were pleictropism or multigenic effect and PPARGC1A and OPN may be the causal mutations behind milk production QTLs on BTA6 in the Chinese Holstein population.
基金supported by the earmarked fund for China Agriculture Research System(CARS-37)the National Key Technologies R&D Program of China(2012BAD12B02)the Special Fund for Agro-scientific Research in the Public Interest(No.201303143)
文摘Background:Phosphorus(P) supplementation is costly and can result in excess P excretion.This study investigated the effects of reducing dietary P on milk production and P excretion in dairy cows over a full lactation.Method:Forty-five multiparous Holstein dairy cows were divided into 15 blocks according to expected calving date and previous milk yield,and assigned randomly to one of the three dietary treatments:0.37,0.47,and 0.57%P(DM basis);these P levels represent the NRC recommendations,Chinese recommendations,and the amount of dietary P commonly fed by Chinese dairy farmers,respectively.Average daily feed intake was calculated from monthly data on feed offered and refused.Milk yields of individual cows were recorded weekly,and milk samples were taken for analysis of protein,fat,solids-not-fat,lactose,and somatic cell count.Blood samples were collected on days-6,-3,0,3,6 relative to calving,and then monthly throughout lactation,and analyzed for P and Ca concentrations.Spot samples of feces and urine were collected for 3 consecutive d during weeks 12,24,and 36,and P concentrations were analyzed.Reproduction and health data were recorded.Results:Dietary P did not affect dry matter intake or milk yield(P〉 0.10).Milk fat content was slightly higher in cows fed 0.37%P than in cows fed 0.47%P(P = 0.05).Serum concentrations of P and Ca did not reflect dietary P content(P〉 0.10).Fecal and urinary P both declined linearly(P〈 0.05) as dietary P decreased from 0.57 to0.37%.Fecal P content was 25%less when dietary P was 0.37%compared to 0.57%.Health events and reproductive performance were not associated with dietary P content(P〉 0.05).Conclusions:Lowering dietary P from 0.57 to 0.37%did not negatively affect milk production,but did significantly reduce P excretion into environment.
基金co-funded by the Dairy Goat Co-operative,Ministry of Business,Innovation&Employment(3709153)the Ministry for Primary Industries Sustainable Food and Fibre Futures Fund(5000835)。
文摘Background:Economically important milk production traits including milk volume,milk fat and protein yield vary considerably across dairy goats in New Zealand.A significant portion of the variation is attributable to genetic variation.Discovery of genetic markers linked to milk production traits can be utilised to drive selection of highperformance animals.A previously reported genome wide association study across dairy goats in New Zealand identified a quantitative trait locus(QTL)located on chromosome 19.The most significantly associated single nucleotide polymorphism(SNP)marker for this locus is located at position 26,610,610(SNP marker rs268292132).This locus is associated with multiple milk production traits including fat,protein and volume.The predicted effect of selection for the beneficial haplotype would result in an average production increase of 2.2 kg fat,1.9 kg protein and 73.6 kg milk yield.An outstanding question was whether selection for the beneficial allele would co-select for any negative pleiotropic effects.An adverse relationship between milk production and udder health traits has been reported at this locus.Therefore,a genome wide association study was undertaken looking for loci associated with udder traits.Results:The QTL and production associated marker rs268292132 was identified in this study to also be associated with several goat udder traits including udder depth(UD),fore udder attachment(FUA)and rear udder attachment(RUA).Our study replicates the negative relationship between production and udder traits with the high production allele at position 19:26,610,610(SNP marker rs268292132)associated with an adverse change in UD,FUA and RUA.Conclusions:Our study has confirmed the negative relationship between udder traits and production traits in the NZ goat population.We have found that the frequency of the high production allele is relatively high in the NZ goat population,indicating that its effect on udder conformation is not significantly detrimental on animal health.It will however be important to monitor udder conformation as the chromosome 19 locus is progressively implemented for marker assisted selection.It will also be of interest to determine if the gene underlying the production QTL has a direct effect on mammary gland morphology or whether the changes observed are a consequence of the increased milk volume.
文摘Goats are one of the oldest domesticated animal species widely distributed in the world playing an important role in the food production system in Sub-Saharan African Region (SSAR). Due to their multiple uses (milk production, meat, fiber and hides) and adaptation aptitudes to ecological conditions, goats produce and contribute positively to farmers’ socio-economy status in various production systems. This review aimed at giving a summary overview on the goat’s production systems characteristics, the genetic diversity and the candidate genes affecting reproductive and milk production performances in goat breeds in SSAR. It has been observed that traditional livestock production system with communal grazing system is the most used in goat keeping in SSAR. The geographical locations play an important role in the relationships between goat’s distributions in the region. At the same time, goats might have been differentiated and isolated one to others due to the wide geographic range, the diversify climate and the topography in the region. Among the six worldwide known haplogroups of goat (A, B, C, D, G and F), haplogroup A is the most representative in SSAR. However, haplogroup G and B can be found in some goat populations in some countries in east (Kenya and Ethiopia) and south parts of Africa. This review reveals that little is known on the candidate genes associated with prolificacy and milk production traits in indigenous goat breeds in the region. That observation suggests the importance of assessing candidate genes associated with economic traits in the populations of goat in SSAR.
基金Project fund by Australian Centre for International Agricultural Research
文摘This paper describes milk production and livestock production in Tibet. Some information of market demand has also been presented. There has been very little information published in Tibetan journals on production and nutrition of cattle. This review provides a brief introduction to feeding systems and feeding resources. Many studies on milk production have been done in isolation, and do not go beyond the basic and practical level. Compared with dairy cattle research in other parts of China, large gaps in knowledge still exist in cattle production science, particularly related to nutrition, and systems approaches for the development of a dairy industry.
基金supported by the National Key Basic Research Program of China (No. 2006CB102104)
文摘To fine map the previously detected quantitative trait loci (QTLs) affecting milk production traits on bovine chromosome 6 (BTA6), 15 microsatellite markers situated within an interval of 14.3 cM spanning from BMS690 to BM4528 were selected and 918 daughters of 8 sires were genotyped. Two mapping approaches, haplotype sharing based LD mapping and single marker regression mapping, were used to analyze the data. Both approaches revealed a quantitative trait locus (QTL) with significant effects on milk yield, fat yield and protein yield located in the segment flanked by markers BMS483 and MNB209, which spans a genetic distance of 0.6 cM and a physical distance of 1.5 Mb. In addition, the single marker regression mapping also revealed a QTL affecting fat percentage and protein percentage at marker DIK2291. Our fine mapping work will facilitate the cloning of candidate genes underlying the QTLs for milk production traits.
基金supported by grants from the National Key Basic Research Program of China(No. 2011CB100803)
文摘Thirty lactating Holstein cows were used to investigate the effects of different forages quality on milk fatty acids(FA) profiles and production. The cows were assigned to 3 dietary treatments(n = 10 per treatment) in a randomized block design with 3 repeated measures. They were fed the experimental diets for 90 d with 3 days of collection of samples for analysis at about 27 d intervals(samples were collected on days 28,29,30, 58, 59, 60.88, 89 and 90). The treatments were(DM basis): 1) mixed forages diet(MF) consisting of 3.7% Chinese wild rye, 26.7% corn silage and 23.4% alfalfa hay; 2) corn stalk diet 1(CS1) where corn stalk was used to formulate a similar chemical nutrient level to MF; 3) corn stalk diet 2(CS2) which used corn stalk to formulate a similar forage level to MF for the diet. Dry matter intake and BW were similar between treatments, but daily milk yield, milk fat and protein yield decreased(P < 0.05)in CS1 and CS2 compared with MF, with CS2 being the lowest(P < 0.05). In total FA of milk, the compositions of C18:1 c9, C18:3 and unsaturated FA increased(P < 0.05) in CS1 and CS2 compared with MF,and C18:0 and trans-C18:1 were trended to increase(P < 0.10), but C4:0-C16:0 were decreased(P < 0.05).Compared with cows fed CS2, cows receiving CS1 increased the compositions of C4:0 to C12:0 and C18:2(P < 0.05). The results suggests feeding corn stalk could produce a greater proportion of unsaturated fatty acid(UFA) in milk fat without resulting in milk fat depression(MFD) in mid lactation cows, but simply increasing the ratio of concentrate in low forages diets is not an effective way to increase milk fat synthesis and milk production.
基金supported by the National High-tech R&D Program of China(2013AA102504)the National Key Technology R&D Program(2011BAD28B02)+3 种基金the National Transgenic Major Project(2014ZX08009-053B)the Beijing Innovation Team of Technology System in the National Dairy Industry,the Beijing Research and Technology Program(D121100003312001)the earmarked fund for Modern Agro-industry Technology Research System(CARS-37)the Program for Changjiang Scholar and Innovation Research Team in University(IRT1191).
文摘Genome-wide association studies with an Illumina Bovine50K chip have detected 105 SNPs associated with one or multiple milk production traits in the Chinese Holstein population.Of these,38 significant SNPs detected with high confidence by both L1-TDT and MMRA methods were selected to further mine potential key genes affecting milk yield and milk composition.By blasting the flanking sequences of these 38 SNPs with the bovine genome sequence combined with comparative genomics analysis,26 genes were found to contain or be near to such SNPs.Among them,the C14H8orf33 gene is merely 87 bp away from the significant SNP,Hapmap30383-BTC-005848.Hence,we report herein genotype-phenotype associations to further validate the genetic effects of the C14H8orf33 gene.By pooled DNA sequencing of 14 unrelated Holstein sires,a total of 18 with seven novel SNPs were identified.Among them,nine SNPs were in the 5′regulatory region,one in exon 6 and the other in the 3′UTR and 3′regulatory region.A total of nine of these identified SNPs were successfully genotyped and analyzed by mass spectrometry for association with five milk production traits in an independent resource population.The results showed that these SNPs were statistically significant for more than two traits[P<(0.0001–0.0267)].In addition,mRNA expression analyses revealed that C14H8orf33 was ubiquitous in eight different tissues,with a relatively higher expression level in the mammary gland than in other tissues.These findings,therefore,provide strong evidence for association of C14H8orf33 variants with milk yield and milk composition traits and may be applied in Chinese Holstein breeding programs.
文摘The objective of this study was to analyze the effect of adding meteorological data to the training process of two milk production forecast models.The two models chosen were the nonlinear auto-regressive model with exogenous input(NARX)and the multiple linear regression(MLR)model.The accuracy of these models were assessed using seven different combinations of precipitation,sunshine hours and soil temperature as additional model training inputs.Lactation data(daily milk yield and days in milk)from 39 pasture-based Holstein-Friesian Irish dairy cows were selected to compare to the model outputs from a central database.The models were trained using historical milk production data from three lactation cycles and were employed to predict the total daily milk yield of a fourth lactation cycle for each individual cow over short(10-day),medium(30-day)and long-term(305-day)forecast horizons.The NARX model was found to provide a greater prediction accuracy when compared to the MLR model when predicting annual individual cow milk yield(kg),with R2 values greater than 0.7 for 95.5%and 14.7%of total predictions,respectively.The results showed that the introduction of sunshine hours,precipitation and soil temperature data improved the prediction accuracy of individual cow milk prediction for the NARX model in the short,medium and long-term forecast horizons.Sunshine hours was shown to have the largest impact on milk production with an improvement of forecast accuracy observed in 60%and 70%of all predictions(for all 39 test cows from both groups).However,the overall improvement in accuracy was small with a maximum forecast error reduction of 4.3%.Thus,the utilization of meteorological parameters in milk production forecasting did not have a substantial impact on forecast accuracy.
文摘The study evaluated the effects of a diet incorporating a mango-based feed on the technical-economic performance of milk production of Montbeliard cows at the end of lactation in a dairy farm in Bobo-Dioulasso, Burkina Faso. To do so, 14 cows were subjected to 3 diets, including an experimental diet incorporating mango (ED) in comparison to a balanced control diet (BCD) and to the feeding practice of the farmer (FCD) during 60 days. Results show that the average amount of milk milked per day from ED (10.93 ± 0.17 L) was significantly higher (p < 0.05) than BCD (8.80 ± 0.19 L) and RTA (5.39 ± 0.14 L). The conversion index of FPD (4.24 ± 0.14) was significantly higher (p < 0.05) than that of BCD (1.75 ± 0.19) and ED (1.26 ± 0.16). Average feed costs per liter of milk (FC/L) were significantly different (p (125.95 ± 4.56 FCFA), BCD (83.67 ± 6.36 FCFA) and ED (60.06 ± 5.49 FCFA). The average gross profit margins (GPM) were significantly higher and decreasing (p ,953 ± 5031 FCFA), BCD (167,157 ± 5831 FCFA) and FCD (74,188 ± 4185 FCFA). Mango feed can be used in the diet of dairy cows. Its use contributes to the reduction of production costs and the generation of substantial profits. The valorization of mango residues is an alternative for a greater availability of feed at lower cost for the animals. It could contribute to a better management of processing residues that pollute the environment and to the reduction of fruit flies that affect orchard yields through reinfestation. The increased use of mango in dairy cattle feed should be encouraged.
基金Financially supported by the Danish Council for Independent Research,Technology and Production Sciences (Copenhagen K,Denmark)。
文摘Background Dietary fat is important for energy provision and immune function of lactating sows and their progeny.However,knowledge on the impact of fat on mammary transcription of lipogenic genes,de novo fat synthesis,and milk fatty acid(FA)output is sparse in sows.This study aimed to evaluate impacts of dietary fat levels and FA composition on these traits in sows.Forty second-parity sows(Danish Landrace×Yorkshire)were assigned to 1 of 5 dietary treatments from d 108 of gestation until weaning(d 28 of lactation):low-fat control diet(3%added animal fat);or 1 of 4 high-fat diets with 8%added fat:coconut oil(CO),fish oil(FO),sunflower oil(SO),or 4%octanoic acid plus 4%FO(OFO).Three approaches were taken to estimate de novo milk fat synthesis from glucose and body fat.Results Daily intake of FA was lowest in low-fat sows within fat levels(P<0.01)and in OFO and FO sows within highfat diets(P<0.01).Daily milk outputs of fat,FA,energy,and FA-derived carbon reflected to a large extent the intake of those.On average,estimates for de novo fat synthesis were 82 or 194 g/d from glucose according to method 1 or 2 and 255 g de novo+mobilized FA/d according to method 3.The low-fat diet increased mammary FAS expression(P<0.05)and de novo fat synthesis(method 1;P=0.13)within fat levels.The OFO diet increased de novo fat synthesis(method 1;P<0.05)and numerically upregulated mammary FAS expression compared to the other high-fat diets.Across diets,a daily intake of 440 g digestible FA minimized milk fat originating from glucose and mobilized body fat.Conclusions Sows fed diets with low-fat or octanoic acid,through upregulating FAS expression,increased mammary de novo fat synthesis whereas the milk FA output remained low in sows fed the low-fat diet or high-fat OFO or FO diets,indicating that dietary FA intake,dietary fat level,and body fat mobilization in concert determine de novo fat synthesis,amount and profiles of FA in milk.
基金funded by the Dairy Goat Co-operative,New Zealand。
文摘Background: Identifying associations between genetic markers and traits of economic importance will provide practical benefits for the dairy goat industry, enabling genomic prediction of the breeding value of individuals, and facilitating discovery of the underlying genes and mutations. Genome-wide association studies were implemented to detect genetic regions that are significantly associated with effects on lactation yields of milk(MY), fat(FY),protein(PY) and somatic cell score(SCS) in New Zealand dairy goats.Methods: A total of 4,840 goats were genotyped with the Caprine 50 K SNP chip(Illumina Inc., San Diego, CA).After quality filtering, 3,732 animals and 41,989 SNPs were analysed assuming an additive linear model. Four GWAS models were performed, a single-SNP additive linear model and three multi-SNP Bayes C models. For the single-SNP GWAS, SNPs were fitted individually as fixed covariates, while the Bayes C models fit all SNPs simultaneously as random effects. A cluster of significant SNPs were used to define a haplotype block whose alleles were fitted as covariates in a Bayesian model. The corresponding diplotypes of the haplotype block were then fit as class variables in another Bayesian model.Results: Across all four traits, a total of 43 genome-wide significant SNPs were detected from the SNP GWAS. At a genome-wide significance level, the single-SNP analysis identified a cluster of variants on chromosome 19 associated with MY, FY, PY, and another cluster on chromosome 29 associated with SCS. Significant SNPs mapped in introns of candidate genes(45%), in intergenic regions(36%), were 0–5 kb upstream or downstream of the closest gene(14%) or were synonymous substitutions(5%). The most significant genomic window was located on chromosome 19 explaining up to 9.6% of the phenotypic variation for MY, 8.1% for FY, 9.1% for PY and 1% for SCS.Conclusions: The quantitative trait loci for yield traits on chromosome 19 confirms reported findings in other dairy goat populations. There is benefit to be gained from using these results for genomic selection to improve milk production in New Zealand dairy goats.
基金Supported by Fund of the National Basic Research Program of China(973)(2011CB100804)
文摘The main purpose of this study was to determine the effect of a corn straw or mixed diet on milk production, milk composition and the expression of genes associated with lactation in mid-lactation Chinese Holstein cows. In this study, 10 healthy Chinese Holstein cows were randomly assigned to two groups and fed with different diets respectively, corn straw (CS) or mixed forage (MF) diet. CS group was fed roughage consisting of 53.8% corn straw only and the forge to concentrate (F : C) ratio [dry matter (DM)] was about 40: 60. MF group was fed roughage consisting of 3.7% Chinese wildrye and 23.4% alfalfa hay, the forge to concentrate (F : C) ratio (DM) was 70: 30. All the cows were fed 8 weeks and body weight, dry matter intake, body condition score, fat, protein, lactose, milk yield, total solid and somatic cell count (SCC) were recorded. Quantitative real-time PCR (qRT-PCR) was used to analyze cow mammary gland samples representing two different diets. The results suggested that different diet types had significant effects on milk yield, lactose, milk fat, milk protein, dry matter intake and somatic cell count in dairy cows, and cows fed MF diet improved milk production and lactation performance clearly (P〈0.05). In addition, mRNA expression of genes ACC, roTOR, STATS, CSN2, PPAR),, FABP3 and PTEN in MF group was extremely significantly higher than that in CS group (P〈0.05). mRNA expression ofAKT1, FAS, SCD and SREBPlc in MF group was significantly higher than that in CS group (P〈0.01). In summary, the milk yield and composition in mixed forage group were significantly improved than those in corn straw group.
文摘[Objective] The paper was to increase utilization rate of roughage to solve current shortage of forage and improve production performance of Simmental cow.[Method] Fruit-flavored agent and green pigment were added in roughage of Simmental cow respectively,and their effects on feed intake,milk production and milk quality of Simmental cow were studied.[Result] After adding fruit-flavored agent and green pigment,the feed intakes of Simmental cow were increased by 30.69% and 12.27%,while the milk productions were increased by 1.74 and 2.25 kg,respectively,and the differences were all significant(P 〈 0.05).[Conclusion]Adding 0.3% and 0.1% fruit-flavored agent and green pigment in roughage of Simmental cow could significantly improve feed intake and milk production,which can also delay the decline of milk production during late lactation stage and improve milk quality.
文摘Objective To develop an analytical method for simultaneously qualitative and quantitative determination of melamine and triazine-related by-products including ammelide, ammeline, and cyanuric acid in milk and milk products by gas chromatography- tandem mass spectrometry (GC-MS/MS). Methods Melamine and triazine-related by-products namely ammelide, ammeline and cyanuric acid in the samples were extracted in a solvent mixture of diethylamine, water, and acetonitrile (10:40:50, V/V/V). After centrifugation, an aliquot of the supernatant was evaporated to dryness under a gentle stream of nitrogen gas, and then melamine and triazine-related by-products were derivatized using BSTFA with 1% TMCS. The derivatives of melamine and its analogues were determined by gas chromatography/tandem mass spectrometry using multiple reactional monitoring (MRM) with 2, 6-Diamino-4-chloropyrimidine (DACP) being used as an internal standard. Results The linear detectable ranges were from 0.004 mg/kg to 1.6 mg/kg for melamine, ammelide, ammeline, and cyanuric acid with a correlation coefficient no less than 0.999. The recovery rates of the tour compounds in spiked blank milk powder at concentrations 0.5, 1, 2 mg/kg were between 61.4%-117.2%, and the relative standard deviation was no more than 11.5% (n=6). The detection limits of melamine, ammelide, ammeline and cyanuric acid in milk powder were 0.002 mg/kg with a ratio of signal to noise of 3. Conclusion This GC-MS/MS method for simultaneous determination of melamine, ammelide, ammeline, and cyanuric acid in milk and milk products is sensitive and specific.
基金Supported by Fund of Post Doctoral Research in Heilongjiang Province(LSZH-04052)Fund of Scientific and Technical Research Projects(10551039)
文摘Scientific and technical progress has been the driving forces of enterprises development.Milk productive enterprises are developing faster and growing better.It is very important to measure the contributive ratio of scientific and technical progress in milk productive enterprises.And the appraisement could help to develop milk productive enterprises.The model C^2GS^2 was established to appraise the contributive ratio of scientific and technical progress in milk productive enterprises in the research.And the appraisement on the contributive ratio of scientific and technical progress in milk productive enterprises was made by the model.In the results of appraisement,science and technology play a main role in milk productive enterprises.It is shown that our milk productive enterprises are developed by scientific and technical progress while not by input of productive factors.
基金supported by the National Natural Science Foundation of China(31972147)Project of Tianjin Science and Technology Plan(19PTSYJC00050)+1 种基金the Open Project Program of State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science and Technology(SKLFNS-KF-202011)Project program of Key Laboratory of Food Nutrition and Safety,Ministry of Education,Tianjin Key Laboratory of Food Nutrition and Safety,China(JYB202002)。
文摘In this study,a label-free,portable and reproducible immunochip based on quartz crystal microbalance(QCM)was developed for the qualitative detection ofβ-lactoglobulin(β-LG),an allergen,in milk products.Experimental parameters in the fabrication and regeneration procedure such as pH of the coupling microenvironment,amount of anti-β-LG antibody and regeneration reagent were optimized in detail.Under optimal conditions,the proposed QCM immunochip exhibited good recognition of β-LG,with a calibration curve of ΔF=12.877 C_(β-LG)^(0.4809)(R^(2)=0.9982)and limit of detection of 0.04μg/mL.Additionally,this portable QCM immunochip had good stability,high specificity,and no obvious cross-reaction to three other milk proteins(α-casein,α-lactalbumin,and lactoferrin).It could compete a qualitative measurement within5 min,and could be reused at least ten times.In the β-LG analysis of actual milk samples,the developed QCM immunochip yielded reliable and accurate results,which correlated strongly with those from the standard HPLC method(R^(2)=0.9969).Thus,the portable,stable,and reproducible QCM immunochip developed in this study allowed the rapid,cost-effectively and sensitively measure theβ-LG in milk products.
文摘<i>Aflatoxin</i> M1 (AFM1) occurrence in milk and milk products has been a major concern among food scientists over the past three decades owing to its possible health risk in humans. The well-documented AFM1 associated adverse health outcomes include hepatocarcinogens, mutagens genotoxins, mutagenicity, and teratogen. The paper reviews the occurrence of AFM1 in milk, milk products, and human milk in developing and developed countries, with its safety standards of regulation. The health associated risks with AFM1 are the important methods used in detecting and reducing AFM1 in milk and other milk products are presented.
文摘Dairy products are nutritious food items that contain various essential nutrients,however,it has been proven that residual antibiotics have contaminated such products.These residues can cause several side effects on human health.They increase antimicrobial resistance against several threatening microorganisms,as well as significant growth in allergenic reactions.Various methods,including heat treatments,have been applied to alleviate and reduce the effect of antibiotic residue level in milk and milk products.Changes in drug levels were not sig-nificantly remarkable,obliging researchers to find new approaches to prevent or reduce their risk and limit their complications on human health.
基金Supported by Key R&D Projects in Hebei Province(19227516D)Hebei Provincial PhaseⅡModern Agricultural Industry Technology System Innovation Team Building Project(HBCT2018120207,HBCT2018160403)Hebei Province Innovation Ability Promotion Plan Project(20567673H)。
文摘Milk and dairy products are more and more popular with consumers due to their various nutrients, and their quality and safety issues have always been concerned. Therefore, the development of rapid, accurate and simple screening techniques is of great significance. Liquid chromatography-high resolution time-of-flight mass spectrometry has high-resolution and high-throughput detection functions, and has gradually begun to be applied in the detection of milk and dairy products. This paper summarized the application of milk and dairy products in liquid chromatography-high resolution time-of-flight mass spectrometry, laying a foundation for the development of new methods.