Objective:To determine the new M-superfamily conotoxins from molluscivorous snail Conus bandanus in Vietnam.Methods:Conus bandanus venom was fractionated and purified on HPLC system with an analytical reversed-phase C...Objective:To determine the new M-superfamily conotoxins from molluscivorous snail Conus bandanus in Vietnam.Methods:Conus bandanus venom was fractionated and purified on HPLC system with an analytical reversed-phase C18 column in order to screen small conotoxins.The primary structure of peptide was analyzed by matrix-assisted laser desorption/ionization time of flight tandem mass spectrometry using collision-induced dissociation and confirmed by Edman’s degradation method.Results:Five new conotoxins were biochemically characterized from the crude venom of the mollusk-hunting cone snail Conus bandanus,which were collected at Ke Ga reef of the Nha Trang Bay(Vietnam).Each conotoxin had 15 or 16 amino acid residues and shared the same characteristic cysteine framework V as–CC–C–C–CC–.They were termed as Bn3 b,Bn3 c,Bn3 d,Bn3 e and Bn3 f following the conotoxins nomenclature.Conclusions:The conotoxins Bn3 b,Bn3 e,and Bn3 f are categorized in the mini-M conotoxins of the M1 branch,while conotoxins Bn3 c and Bn3 d are categorized in the mini-M conotoxins of the M2 branch.The homological analysis reveals that these conotoxins could serve as promising probe compounds for voltage-gated sodium channels.展开更多
目的比较简明国际神经精神访谈(轻)躁狂发作伴混合特征[Mini International Neuropsychiatric Interview(Hypo-)Manic Episode with Mixed Features-DSM-5 Module,MINI-M]问卷和临床实用抑郁发作伴混合特征量表(Clinically Useful Depre...目的比较简明国际神经精神访谈(轻)躁狂发作伴混合特征[Mini International Neuropsychiatric Interview(Hypo-)Manic Episode with Mixed Features-DSM-5 Module,MINI-M]问卷和临床实用抑郁发作伴混合特征量表(Clinically Useful Depression Outcome Scale Supplemented with Questions for the DSM-5 Mixed Features Specifier,CUDOS-M)抑郁分量表识别(轻)躁狂发作伴混合特征的有效性。方法在全国多中心、大样本调查中采取方便抽样方法纳入符合入组与排除标准的366例急性期(轻)躁狂发作的双相障碍患者,依据DSM-5混合特征的症状学标准进行临床诊断,采用受试者操作特征曲线(receiver operating characteristic curve,ROC)分析MINI-M问卷和CUDOS-M抑郁分量表识别混合特征的预测效度并比较二者ROC曲线下面积(area under the curve,AUC)差异性。结果MINI-M问卷和CUDOS-M抑郁分量表预测混合特征的AUC分别为0.79(95%CI=0.75~0.84)、0.81(95%CI=0.77~0.86),二者AUC差异无统计学意义(Z=-1.19,P>0.05)。依据DSM-5诊断标准,(轻)躁狂发作患者中混合特征临床诊断率为45.9%(168/366),MINI-M问卷(得分≥3分)和CUDOS-M抑郁分量表(得分≥20分)检出率分别为43.7%(160/366)和42.1%(154/366),三者具有可比性。结论(轻)躁狂发作患者中混合特征普遍存在,MINI-M问卷与CUDOS-M抑郁分量表识别混合特征的效度相当。展开更多
基金funded by Vietnam National Foundation for Science and Technology Development(NAFOSTED)under grant number 106-NN.02-2015.14
文摘Objective:To determine the new M-superfamily conotoxins from molluscivorous snail Conus bandanus in Vietnam.Methods:Conus bandanus venom was fractionated and purified on HPLC system with an analytical reversed-phase C18 column in order to screen small conotoxins.The primary structure of peptide was analyzed by matrix-assisted laser desorption/ionization time of flight tandem mass spectrometry using collision-induced dissociation and confirmed by Edman’s degradation method.Results:Five new conotoxins were biochemically characterized from the crude venom of the mollusk-hunting cone snail Conus bandanus,which were collected at Ke Ga reef of the Nha Trang Bay(Vietnam).Each conotoxin had 15 or 16 amino acid residues and shared the same characteristic cysteine framework V as–CC–C–C–CC–.They were termed as Bn3 b,Bn3 c,Bn3 d,Bn3 e and Bn3 f following the conotoxins nomenclature.Conclusions:The conotoxins Bn3 b,Bn3 e,and Bn3 f are categorized in the mini-M conotoxins of the M1 branch,while conotoxins Bn3 c and Bn3 d are categorized in the mini-M conotoxins of the M2 branch.The homological analysis reveals that these conotoxins could serve as promising probe compounds for voltage-gated sodium channels.
文摘目的比较简明国际神经精神访谈(轻)躁狂发作伴混合特征[Mini International Neuropsychiatric Interview(Hypo-)Manic Episode with Mixed Features-DSM-5 Module,MINI-M]问卷和临床实用抑郁发作伴混合特征量表(Clinically Useful Depression Outcome Scale Supplemented with Questions for the DSM-5 Mixed Features Specifier,CUDOS-M)抑郁分量表识别(轻)躁狂发作伴混合特征的有效性。方法在全国多中心、大样本调查中采取方便抽样方法纳入符合入组与排除标准的366例急性期(轻)躁狂发作的双相障碍患者,依据DSM-5混合特征的症状学标准进行临床诊断,采用受试者操作特征曲线(receiver operating characteristic curve,ROC)分析MINI-M问卷和CUDOS-M抑郁分量表识别混合特征的预测效度并比较二者ROC曲线下面积(area under the curve,AUC)差异性。结果MINI-M问卷和CUDOS-M抑郁分量表预测混合特征的AUC分别为0.79(95%CI=0.75~0.84)、0.81(95%CI=0.77~0.86),二者AUC差异无统计学意义(Z=-1.19,P>0.05)。依据DSM-5诊断标准,(轻)躁狂发作患者中混合特征临床诊断率为45.9%(168/366),MINI-M问卷(得分≥3分)和CUDOS-M抑郁分量表(得分≥20分)检出率分别为43.7%(160/366)和42.1%(154/366),三者具有可比性。结论(轻)躁狂发作患者中混合特征普遍存在,MINI-M问卷与CUDOS-M抑郁分量表识别混合特征的效度相当。
