Inhibitory Effect of Wood Vinegar Produced from Apricot Shell on Aspergillus fumigatus

[Objectives] This study was conducted to explore the antifungal activity of wood vinegar produced from apricot shell. [Methods]The minimal inhibitory concentration（ MIC） of wood vinegar against Aspergillus fumigatus was determined on agar plates. Then,the mycelial growth and the morphology of the apical vesicles of A. fumigatus cultured in the medium supplemented with 1/2 MIC wood vinegar were observed to evaluate the effect of wood vinegar on A. fumigatus.RNA-Seq was performed to explore the antifungal mechanism of wood vinegar. [Results] The wood vinegar produced from apricot shell could inhibit the growth of A. fumigatus,with a MIC of 20 μl/ml. In the medium supplemented with 1/2 MIC wood vinegar,the mycelial growth of A. fumigatus was suppressed,some apical vesicles were incompletely developed. The gene ontology（ GO） term enrichment analysis based on RNA-Seq showed that the differentially expressed genes（ DEGs）were enriched in metabolic process,cellular process and single-organism process in the category of biological processes,cell,cell part,membrane,organelle and membrane part in the category of cellular components,catalytic activity and binding in the category of molecular function. The significantly up-regulated DEGs were mostly involved in cell,cell part,organelle,organelle part and macro molecular complex in the category of cellular component,while the significantly down-regulated DEGs were mostly involved in biological process,cellular process and single-organism process in the category of biological process,catalytic activity,binding,transporter activity,and nucleic acid binding transcription factor activity in the category molecular function. Moreover,KEGG（ Kyoto Encyclopedia of Genes and Genomes） pathway analysis showed that the DEG unigenes involved in metabolism were the most,followed by those involved in genetic information processing.[Conclusions]The results indicated that the wood vinegar produced from apricot shell might inhibit the growth of A. fumigatus by interfering with the expression of multiple genes involved in multiple signaling pathways.

Synthesis,Spectroscopy and Antibacterial Activity of Supermolecular Compounds of Organotitanium Substituted Heteropolytungstates Containing 8-Quinolinol

Parent compounds of cyclopentadienyltitanium substituted heteropolytungstates with Keggin structure,An[(CpTi)XW11O39]·xH2O(A=Me4N,K;X=P,Si,Co;Cp=η5-C5H5) were synthesized in aqueous phase.By allowing parent heteropoly compounds to react with protonated 8-quinolinol,the title supermolecular compounds(C9H8NO)mAn[(CpTi)XW11O39]·xH2O(A=Me4N,H;X=P,Si,Co) were synthesized.The title compounds were characterized by means of elementary analysis,IR,UV,1H NMR,XRD and TG-DSC.The results indicate that the title compounds are new heteropoly compounds,and there is a charge transfer interaction between the organic cation and heteropoly anion.The results obtained from thermal analysis show that QCpTiPW,QCpTiSiW and QCpTiCoW begin to decompose at 212.4,194.2 and 171.2 ℃,respectively.The results obtained from antibacterial test reveal that QCpTiSiW has the best antibacterial activity,and the MIC values of QCpTiSiW against Escherichia coli and Staphylococcus aurous are 64.0 and 0.500 μg·mL-1,respectively.

Microdilution inhibition test of Chinese herbs to assess their effect against clinical strains of Ureaplasma urealyticum in vitro

Objective：To explore the antibacterial effect of Chinese crude drugs against clinical strains of Ureaplasma urealyticum（UU）, including eight pure herbs and three compound herbs, and determine their minimal inhibitory concentrations（MICs）. Methods：Isolates were collected from clinical patients with UU infection, and cultured in UU broth. In order to test the different effects on clinical strains of UU, the assays were performed by microdilution inhibition tests, and MICs of the herbs against the clinical strains of UU were calculated. Results：The MICs of eight pure herbs against clinical strains of UU were as follows： Galla Chinensis：0.313-1.25 g/L; Fructus Forsythiae： 1.25-5.00 g/L; Cortex Phellodendri： 1.25-5.00 g/L; Radix Paeoniae Rubra： 1.25-2.50 g/L; Semen Plantaginis：2.50-10.00 g/L; Herba Lysimachiae： 5.00-20.00 g/L; Rhizoma Coptidis： 5.00-20.00g/L, Herba Houttuyniae： 10.00-20.00 g/L. The MICs of compound herbs were： Liuheji： 0.625-2.50 g/L; Bazhengsan： 5.00-20.00 g/L; Wulinsan：2.50-20.0 g/L.Conclusion：Galla Chinensis, Fructus Forsythiae, Cortex Phellodendrim, Radix Paeoniae Rubra, and Semen Plantaginis, exerted the stronger antibacterial effect against clinical strains of UU, whereas Herba Lysimachiae, Rhizoma Coptidis and Herba Houttuyniae, had relatively weaker activity against UU. Compound herbs, Bazhengsan and Wulinsan, and particularly Liuheji, also had antibacterial effects against UU. Further studies of the effects and mechanisms of action of Chinese crude drugs against UU infections are worthwhile.

Antibacterial Effect of Herba paederiae and Kalimeris indica (L.) Sch.-Bip Combined with Antibiotics

[ Objective] TO observe the in-vitro antibacterial activity of Herba paederiae and Kalimeds indica （ L. ） Sch.-Bip and their antibacterial effect when being used with commonly used antibiotics. [ Method ] Staphylococcus aureus CMCC29178 and Escherichia coli ATCC25922 reference strains were used for the determination of minimal inhibitory concentration （MIC） and fractional inhibitory concentration （FIC） index using the broth microdilution method. [ Result] The aqueous extracts of Herba paederiae and Kalimeris indica （ L. ） Sch. -Bip had certain antibacterial effects on Staphylococcus aureus and Eschedchia coli. Synergistic effects or additive effects at different degrees were observed when they were applied with the commonly used antibiotics. [ Conclusion] Water extraction can be widely used to primarily screen medicinal plants with antibacterial activity.

Antifungal Activities of Commercial Rice Wine Extracts of Taiwan Residents Allium fistulosum

Antifungal activities of the commercial rice wine extracts of Allium fistulosum were analyzed. Antifungal activities were tested against 7 pathogenic fungi by using agar disc diffusion and tube dilution tests. The results show that the commercial rice wine extracts of Allium fistulosum have strong antifungal activity against Aspergillus brasiliensis ATCC 16404, Candida albicans ATCC 10231, Microsporumcanis ATCC 36299, M. gypseum ATCC 24102, Trichophyton mentagrophytes ATCC 9533, T. rubrum ATCC 28188, and T. tonsurans ATCC 28942. The commercial rice wine extracts of different A. fistulosum parts were found to exhibit significant antifungal activities with the minimal inhibitory concentration (MIC) in the range of 0.2 - 1.0 mg/mL. The antifungal activity of the extracts of different A. fistulosum parts was in the order of AFS (stem) > AFI (plant body) > AFL (leaf) > AFR (root).

Flash microbiocide:A Rapid and Economic Method for Determination of MBC and MFC

Although nowadays there are methods for determining the Minimal Bactericidal Concentration and Minimal Fungicidal Concentration, it is indispensable that?the establishment of innovative methodologies?could be more practical and cheaper. The new methodology Flash microbiocide is an assay in which one aliquot from 96 well plate of Minimal Inhibitory Concentration test is transferred to another plate containing different culture medium. The correspondence with the reference methods described in the National Committee for Clinical Laboratory Standards (NCCLS-CLSI) document M26-A was achieved, denoting the efficiency of this fast and simple method.

Antibacterial and Antioxidant Activity of Rhodomyrtus Tomentosa and Cinnamomum Zeylanicum Crude Extracts

The aim of this study was to investigate the extraction method for R.to­mentosa and C.zeylanicum leaves and the evaluation of antibacterial and antioxidant activities of crude extracts.The results of the study showed that the active ingredients of crude extracts were clearly separated by Thin-layer chromatography and the presence of rhodomyrtone in R.tomentosa crude extract and cinnamaldehyde in C.zeylanicum crude ex­tract.R.tomentosa crude extract was antibacterial activity against Staph­ylococcus aureus with 13.1 mm of inhibition zone,but is not effective against Salmonella Typhimurium.C.zeylanicum leaf extract did not show antibacterial activity on both S.aureus and S.Typhimurium.At a dilution of 1/2 of the R.tomentosa crude extract can completely inhibit S.aureus growth.This study also indicated the presence of antioxidant compounds such as flavonoids,tannins,phenols and terpenoids in C.zeylanicum and R.tomentosa crude extracts.The results showed that R.tomentosa and C.zeylanicum crude extracts should be used as a biotherapy alternative to antibiotic therapy.However,further study would be needed to investigate the antibacterial activity of crude extracts in vivo.

Triazole Resistance in Aspergillus fumigatus Clinical Isolates Obtained in Nanjing, China

Background： During the past decades, the incidence of invasive aspergillosis （IA） caused by Aspergillus funtigatus has increased dramatically. The aims of this study were to investigate the susceptibility of clinical isolates of A.fumigatus to triazole and the underlying cyp51A mutations in triazole-resistant A. fumigatus. Methods： A total of 126 A.Jumigatus clinical isolates from 126 patients with proven or probable IA were obtained from four large tertiary hospitals in Nanjing, China, between August 2012 and July 2015. The determination of minimal inhibitory concentrations （MICs） for itraconazole, voriconazole, and posaconazole was performed by broth microdilution according to the European Committee on Antimicrobial Susceptibility Testing reference method. Results： A total of 4 A.fumigatus isolates （3.17%） were confinned to be itraconazole resistant, with MICs of ≥8 mg/L, and one isolate （0.8%） was confirmed to be voriconazole resistant and posaconazole resistant, with MICs of 4 mg/L and 0.5 mg/L, respectively. We found that two of the 4 isolates of triazole-resistant A. fumigatus had the L98H amino acid substitution in combination with a 34-base pair tandem repeat in the promoter region, one isolate had an M2201 mutation, and another itraconazole-resistant isolate did not have a substitution in the civp51A gene. Conclusions： This study shows that triazole-resistant A.fumigatus clinical isolates are present in Nanjing, China, which is a new challenge to the clinical management of IA.

Molecular characterization and antibacterial effect of endophytic actinomycetes Nocardiopsis sp. GRG1 (KT235640) from brown algae against MDR strains of uropathogens

Our study is to evaluate the potential bioactive compound of Nocardiopsis sp.GRG1(KT235640)and its antibacterial activity against multi drug resistant strains(MDRS)on urinary tract infections(UTIs).Two brown algae samples were collected and were subjected to isolation of endophytic actinomycetes.100 strains of actinomycetes were isolated from algal samples based on observation of morphology and physiological characters.40 strains were active in antagonistic activity against various clinical pathogens.Among the strains,10 showed better antimicrobial activity against MDRS on UTIs.The secondary metabolite of Nocardiopsis sp.GRG1(KT235640)has showed tremendous antibacterial activity against UTI pathogens compared to other strains.Influence of various growth parameters were used for synthesis of secondary metabolites,such as optimum pH 7,incubation time 5e7 days,temperature(30
C),salinity(5%),fructose and mannitol as the suitable carbon and nitrogen sources.At 100 μg/ml concentration MIC of Nocardiopsis sp.GRG1(KT235640)showed highest percentage of inhibition against Proteus mirabilis(85%),and E.coli,Staphylococcus auerues,Psuedomonas aeroginasa,Enterobactor sp and Coagulinase negative staphylococci 78e85%respectively.

Synthesis and biological evaluation of dihydrotriazine derivatives as potential antibacterial agents

A series of 1,4-dihydro-1,3,5-triazine derivatives were designed and synthesized and their antibacterial and antifungal activities were evaluated. Most of the synthesized compounds showed potent inhibition of several Gram-positive bacterial strains（including multidrug-resistant clinical isolates） and Gramnegative bacterial strains, with minimum inhibitory concentrations（MICs） in the range of 2.1–181.2 mmol/L. Compounds 7a and 7c presented the most potent inhibitory activities against Grampositive bacteria（e.g., Staphylococcus aureus 4220）, Gram-negative bacteria（e.g., Escherichia coli 1924）,and the fungus Candida albicans 7535, with MICs of 2.1 or 4.1 mmol/L. Especially, compound 7a was the most potent, with an MIC of 2.1 mmol/L against four multidrug-resistant, Gram-positive bacterial strains.The cytotoxic activity of the compound 7a, 7c and 7f was assessed in HepG2 cells, and the results suggest that 1,4-dihydro-1,3,5-triazine derivatives bearing a 6-benzyloxynaphthalen moiety are interesting scaffolds for the development of novel antibacterial agents.

Prevalence of Edwardsiella tarda in commercially important finfish and shellfish of Bihar and West Bengal,India

Objective:To study the prevalence of Edwardsiella tarda(E.tarda)in finfish and shellfish of West Bengal and Bihar,India and their antibiogram.Methods:Fish samples were enriched overnight in Edwardsiella ictaluri broth and plated onto Edwardsiella ictaluri agar.Typical colonies were identified conventionally and by VITEK 2 compact system.Antibiogram was done by agar disc diffusion assay.Results:Of the 118 fish samples screened,only 14.41%had E.tarda.The incidence was marginally high in wild fish(15.30%)than in cultured fish(13.26%).Maximum incidence was in intestine(12.98%)followed by gills(6.60%)and skin(2.38%).All E.tarda strains were sensitive to ciprofloxacin and exhibited varying degrees of resistance to other antibiotics.Multiple antibiotic resistance was seen in 84.00%–87.50%of the E.tarda strains.Majority of them had high minimal inhibitory concentration values(200μg/mL)for oxytetracycline and gentamycin.Conclusions:The results suggested that considerable proportions of commercially important finfish are carriers of multiple antibiotic resistance E.tarda.This calls for proper sanitary measures to eliminate this pathogen in fish and fishery products.