[Objective] The study was to immunohistochemical localize the endocrine cells from digestive tract of Misgurnus [ Method ] Using six gastrointestinal hormone antisera, the endocrine cells from digestive tract of M. an...[Objective] The study was to immunohistochemical localize the endocrine cells from digestive tract of Misgurnus [ Method ] Using six gastrointestinal hormone antisera, the endocrine cells from digestive tract of M. anguillicaudatus was localized. [ Result ]The 5-hydroxytryptamine immunoreactive（5-HT-IR） cells distribute in oesophagus, foregut and midgut; the distribution density was determined to be forepart of foregut 〉 oesophagus and hindpart of foregut 〉 gut, and the differences in the three density gradients reached significant level. Like PP-IR, SS-IR cells were observed mostly in oesophagus, followed by hindpart of foregut, least in forepart of foregut, but never found in gut and hindgut. The three kinds of immunocompetent cells Gas-IR, Glu-IR and SP-IR were not detected in each part of digestive tract. [ Conctusion] This study may provide basic data for studying the nutritional and digestive physiology, as well as the preparation of meridic diets for M. anguillicaudatus.展开更多
Loach （Misgurnus anguillicaudatus） is a commercially important fish in China and an ideal aquaculture species. However, culturists experience high larval and juvenile mortality during mass production. To provide ins...Loach （Misgurnus anguillicaudatus） is a commercially important fish in China and an ideal aquaculture species. However, culturists experience high larval and juvenile mortality during mass production. To provide insight into ways to improve larviculture techniques, we describe the morphological characteristics and behavior of loach during the larval and early juvenile stages. Yolksae larvae ranged from 2.8 to 4.0 mm body length （BL） between days 0 to 4; preflexion larvae ranged from 3.6 to 5.5 mm BL between days 4 to 6; flexion larvae ranged from 4.8 to 8.1 mm BL between days 5 and 14; and post-flexion larvae ranged from 7. l to 15.7 mm BL between days 11 to 27; the minimum length and age of juveniles was 14.1 mm BL and 23 d, respectively. Loach are demersal from hatch through to the early juvenile stages. A suite of morphological characteristics （e.g., external gill filament and ventral mouth opening） and behavioral traits have developed to adapt to demersal living. We observed positive allometric growth in eye diameter, head length, head height, and pectoral fin length during the early larval stages, reflecting the priorities in the development of the organs essential for survival. Our results provide a basis for developing techniques to improve the survival of larval and juvenile loach during mass production.展开更多
Maggot meal was used to replace 0, 20% , 40% , 60% , 80% , 100% of the fish meal in the hasal feed, getting 6 kinds of feed with the same nitrogen content and equal energy （marked as group H0, H20, H40, H60, H80, H10...Maggot meal was used to replace 0, 20% , 40% , 60% , 80% , 100% of the fish meal in the hasal feed, getting 6 kinds of feed with the same nitrogen content and equal energy （marked as group H0, H20, H40, H60, H80, H100）, which were used to feed the Misgurnus anguillicaudatus （loach） for60 d. The effects of the fish meal replacement by maggot meal on the growth performance of M. anguiUicaudatus were studied by comparing the growth performances and body indica- tors of M. anguillicaudatus fed with different feed groups. The results showed that the final weight, weight gain rate, specific growth rate of the M. anguillicaudatus in group H40 showed no significant difference with the control P 〉0.05 ）, but was significantly higher than that of other groups P 〈0.05 ）. Moreover, except group H40, the feed coefficients of all other groups were significantly higher than that in control group （P 〈0.05 ）. The M. anguillicaudatus in group 1-140 had the highest condition factor and COR and the lowest viscera index, all of which showed significant differences with the other replacement groups （P 〈0.05） but the difference with the control group was not significant （P 〉 0.05）. Thus, a proportion of 40% of the fish meal replaced with maggot meal in the mixed feed for the M. anguilli- caudatus could improve the growth performance and physique indexes of M. anguillicaudatus.展开更多
Using the primers which specially amplify the conservative motif of Human SRYgene,we stud ied the PCR amplification of Sox genes in genomic DNA of two species of mud loach Misgurus anguillicau-datus and Paramisgurnus ...Using the primers which specially amplify the conservative motif of Human SRYgene,we stud ied the PCR amplification of Sox genes in genomic DNA of two species of mud loach Misgurus anguillicau-datus and Paramisgurnus dabryanus.Four bands with the length of 200,550,940 and 1000 bp respectively,were presented in the PCR products of Misgumus anguillicaudatus.Three bands with the length of 200,550 and 900 bp were presented in that of Paramisgumus dabryamus.Southern blotting results indicated that the 200 and 550 bp bands are specially positive.There is no difference between male and female individuals as well as between these two species.展开更多
Loach exhibit conspicuous drilling behaviors in the mud of shallow waters,yet their burrow morphology and the factors affecting this behavior have received little attention.We characterized the burrow morphology and w...Loach exhibit conspicuous drilling behaviors in the mud of shallow waters,yet their burrow morphology and the factors affecting this behavior have received little attention.We characterized the burrow morphology and water quality of the pond loach Misgurnus anguillicaudatus in three scenarios:in tanks without plants,tanks with the free-floating plant water hyacinth Eichhornia crassipes,and tanks with water lettuce Pistia stratiotes.Water hyacinth effectively removed water TN,COD,NO3-N and NH4-N,and water lettuce removed water TP and NH4-N.Water hyacinth and water lettuce markedly reduced water turbidity and DO,increased TOC and EC.Water hyacinth purified water more effectively than water lettuce,providing a suitable habitat for loach feeding,living and burrowing.The burrow structures were V-shaped,Y-shaped,inverted L-shaped,or complicated dendritic networks composed of multiple V shapes.The hyacinth treatment was characterized by the greatest burrow volume,length,depth,and structural complexity,but the opening size was reduced by dense root mat coverage.Burrows in the water lettuce treatment were characterized by intermediate volume,length,branches and sinuosity,but they had the largest opening and pit size.The control treatment had a flat bottom with the smallest,shortest burrows.This study indicates that free-floating plants improve habitat suitability and change burrow morphology and may be used to improve loach breeding methods。展开更多
The effects of heavy metals Cd^(2+),Pb^(2+)and Zn^(2+)at 0.05,0.5 and 5.0 mg/L level and their interactions at 0.5 mg/L level on DNA damage in hepatopancreas of loach Misgurnus anguillicaudatus for 1-35 days exposure ...The effects of heavy metals Cd^(2+),Pb^(2+)and Zn^(2+)at 0.05,0.5 and 5.0 mg/L level and their interactions at 0.5 mg/L level on DNA damage in hepatopancreas of loach Misgurnus anguillicaudatus for 1-35 days exposure were examined by single cell gel electrophoresis(SCGE).For each test group,20 loaches with similar body size(5.17-7.99 g;11.79-13.21 cm)were selected and kept in aquaria with dechlori-nated water at(22±1)℃and fed a commercial diet every 48 h.According to the percentage of damaged DNA with tail and its TL/D(tail length to diameter of nucleus)value,the relationship between DNA damage degree and heavy metal dose and exposure time was determined.Results showed that the percentage of damaged DNA and the TL/D value were increased with the prolonged exposure time.The highest percentage(84.85%)of damaged DNA was shown in 5.0 mg/L Zn^(2+)group after 28 days exposure and the biggest TL/D value(2.50)in all treated groups after 35 days exposure.During the first treated week,the damnification of DNA was mainly recognized as the first level,after that time,the third damaged level was mostly observed and the percentage of damaged DNA was beyond 80%.The joint toxic effects among Cd^(2+),Pb^(2+)or Zn^(2+)revealed much complexity,but it generally displayed that the presence of Cd^(2+)could enhance the genotoxicity of Pb^(2+)or Zn^(2+).In conclusion,the results suggestedthattherewasasignificanttime-anddose-depended relationship between the heavy metal and DNA damage in hepatopancreas of loach,and SCGE could represent a useful means to evaluate the genotoxicity of environmental contamination on aquatic organisms.展开更多
This study compared the accumulation of Zn2+and Cu2+in the ovaries and ova of loaches under different concentrations of Zn2+(1.00, 2.50 and 5.00 mg/L respectively) and Cu2+(0.10, 0.25 and 0.50 mg/L respectively). The ...This study compared the accumulation of Zn2+and Cu2+in the ovaries and ova of loaches under different concentrations of Zn2+(1.00, 2.50 and 5.00 mg/L respectively) and Cu2+(0.10, 0.25 and 0.50 mg/L respectively). The results showed that both Zn2+and Cu2+accumulated in the ovaries, and that the relationship between accumulation and time was linear over 20 days of exposure. The accumulation of the metals in ovaries was closely related to the concentration of exposure in the solutions(P<0.05), and was obviously affected by the time and doses. However, the Cu2+concentration was significantly higher than Zn2+(P<0.05). The development level of ova in the ovaries also correlated with the concentration and exposure period in the Zn2+and Cu2+solutions.展开更多
We used microsatellite markers to investigate the genetic parameters of three different polyploidy populations of Misgurnus anguillicaudatus from Honghu City, Hubei Province. Polyacrylamide gel electrophoresis banding...We used microsatellite markers to investigate the genetic parameters of three different polyploidy populations of Misgurnus anguillicaudatus from Honghu City, Hubei Province. Polyacrylamide gel electrophoresis banding patterns of diploid（2n）, triploid（3n） and tetraploid loaches（4n） were analyzed with Pop Gen software. A total of 68 alleles were obtained from seven microsatellite loci and the polymorphism information content（PIC） indices were all above 0.5. The average expected mean heterozygosity values（He） were 0.8420, 0.7186 and 0.8521; the average observed mean heterozygosity values（Ho） were 0.9674, 0.9785 and 0.8928; and the HardyWeinberg P values were 0.3078, 0.3151 and 0.3762, for diploid, triploid and tetraploid individuals, respectively. The results indicated that the three populations were highly polymorphic, with no deviations from Hardy-Weinberg equilibrium observed at all the seven microsatellite loci. This indicated a high level of genetic diversity within the populations. A cluster analysis diagram showed that the shortest genetic distance was between diploid and tetraploid loaches and they shared a close phylogenetic relationship. The triploid and tetraploid individuals had the most distant phylogenetic relationship.展开更多
This study compared the accumulation of Zn^(2+)and Cu^(2+)in the ovaries and ova of loaches under different concentrations of Zn^(2+)(1.00,2.50 and 5.00 mg/L respectively)and Cu^(2+)(0.10,0.25 and 0.50 mg/L respective...This study compared the accumulation of Zn^(2+)and Cu^(2+)in the ovaries and ova of loaches under different concentrations of Zn^(2+)(1.00,2.50 and 5.00 mg/L respectively)and Cu^(2+)(0.10,0.25 and 0.50 mg/L respectively).The results showed that both Zn^(2+)and Cu^(2+)accumulated in the ovaries,and that the relationship between accumulation and time was linear over 20 days of exposure.The accumulation of the metals in ovaries was closely related to the concentration of exposure in the solutions(P<0.05),and was obviously affected by the time and doses.However,the Cu^(2+)concentration was significantly higher than Zn^(2+)(P<0.05).The development level of ova in the ovaries also correlated with the concentration and exposure period in the Zn^(2+)and Cu^(2+)solutions.展开更多
A novel oligosaccharide was isolated and purified from the mucus of the loach, Misgurnus anguillicaudatus. It was identified by several qualitative tests and characterized by elementary analysis, UV and IR spectrum. ...A novel oligosaccharide was isolated and purified from the mucus of the loach, Misgurnus anguillicaudatus. It was identified by several qualitative tests and characterized by elementary analysis, UV and IR spectrum. Its average molecular weight (Mw=1539.4) was determined by gel permeation chromatography. The major structural monomers of Misgurnus anguillicaudatus oligosaccharide were identified to be D-galactose and L-fucose by paper chromatography and gas chromatography.展开更多
A novel antimicrobial polypeptide was isolated and characterized from loach, Misgurnus anguillicaudatus. The polypeptide, named MAPP, is a single-chain polypeptide with Mw about 9,800 Dalton and pI about 4.78; the N-...A novel antimicrobial polypeptide was isolated and characterized from loach, Misgurnus anguillicaudatus. The polypeptide, named MAPP, is a single-chain polypeptide with Mw about 9,800 Dalton and pI about 4.78; the N-tag of MAPP was CFGWN. MAPP showed good inhibition against various bacteria including Bacillus subtilis, Escherichia coli and Staphylococcus aureus. MAPP could be used as a lead compound in antibiotics drug discovery.展开更多
基金Supported by Anhui Key Laboratory of Plant Resources and Biology~~
文摘[Objective] The study was to immunohistochemical localize the endocrine cells from digestive tract of Misgurnus [ Method ] Using six gastrointestinal hormone antisera, the endocrine cells from digestive tract of M. anguillicaudatus was localized. [ Result ]The 5-hydroxytryptamine immunoreactive（5-HT-IR） cells distribute in oesophagus, foregut and midgut; the distribution density was determined to be forepart of foregut 〉 oesophagus and hindpart of foregut 〉 gut, and the differences in the three density gradients reached significant level. Like PP-IR, SS-IR cells were observed mostly in oesophagus, followed by hindpart of foregut, least in forepart of foregut, but never found in gut and hindgut. The three kinds of immunocompetent cells Gas-IR, Glu-IR and SP-IR were not detected in each part of digestive tract. [ Conctusion] This study may provide basic data for studying the nutritional and digestive physiology, as well as the preparation of meridic diets for M. anguillicaudatus.
基金Supported by the Key Technology Research and Development Program of Huai’an(Nos.SN12100 and SN12101)the Construction of Innovation Carrier and Platform Project of Huai’an for the Key Laboratory of Sustainable Fisheries and Environmental Protection for Lakes of Northern Jiangsu(No.HAP201204)the Special Research Fund for Public Interest of MWR of China(No.201201028-02)
文摘Loach （Misgurnus anguillicaudatus） is a commercially important fish in China and an ideal aquaculture species. However, culturists experience high larval and juvenile mortality during mass production. To provide insight into ways to improve larviculture techniques, we describe the morphological characteristics and behavior of loach during the larval and early juvenile stages. Yolksae larvae ranged from 2.8 to 4.0 mm body length （BL） between days 0 to 4; preflexion larvae ranged from 3.6 to 5.5 mm BL between days 4 to 6; flexion larvae ranged from 4.8 to 8.1 mm BL between days 5 and 14; and post-flexion larvae ranged from 7. l to 15.7 mm BL between days 11 to 27; the minimum length and age of juveniles was 14.1 mm BL and 23 d, respectively. Loach are demersal from hatch through to the early juvenile stages. A suite of morphological characteristics （e.g., external gill filament and ventral mouth opening） and behavioral traits have developed to adapt to demersal living. We observed positive allometric growth in eye diameter, head length, head height, and pectoral fin length during the early larval stages, reflecting the priorities in the development of the organs essential for survival. Our results provide a basis for developing techniques to improve the survival of larval and juvenile loach during mass production.
基金Supported by the College Project of Xuzhou Vocational College of Bioengineering(2014B07)
文摘Maggot meal was used to replace 0, 20% , 40% , 60% , 80% , 100% of the fish meal in the hasal feed, getting 6 kinds of feed with the same nitrogen content and equal energy （marked as group H0, H20, H40, H60, H80, H100）, which were used to feed the Misgurnus anguillicaudatus （loach） for60 d. The effects of the fish meal replacement by maggot meal on the growth performance of M. anguiUicaudatus were studied by comparing the growth performances and body indica- tors of M. anguillicaudatus fed with different feed groups. The results showed that the final weight, weight gain rate, specific growth rate of the M. anguillicaudatus in group H40 showed no significant difference with the control P 〉0.05 ）, but was significantly higher than that of other groups P 〈0.05 ）. Moreover, except group H40, the feed coefficients of all other groups were significantly higher than that in control group （P 〈0.05 ）. The M. anguillicaudatus in group 1-140 had the highest condition factor and COR and the lowest viscera index, all of which showed significant differences with the other replacement groups （P 〈0.05） but the difference with the control group was not significant （P 〉 0.05）. Thus, a proportion of 40% of the fish meal replaced with maggot meal in the mixed feed for the M. anguilli- caudatus could improve the growth performance and physique indexes of M. anguillicaudatus.
文摘Using the primers which specially amplify the conservative motif of Human SRYgene,we stud ied the PCR amplification of Sox genes in genomic DNA of two species of mud loach Misgurus anguillicau-datus and Paramisgurnus dabryanus.Four bands with the length of 200,550,940 and 1000 bp respectively,were presented in the PCR products of Misgumus anguillicaudatus.Three bands with the length of 200,550 and 900 bp were presented in that of Paramisgumus dabryamus.Southern blotting results indicated that the 200 and 550 bp bands are specially positive.There is no difference between male and female individuals as well as between these two species.
基金This study was financially supported by the Natural Science Foundation of the Science and Technology Commission of Shanghai Municipality(Grant No.13ZR1427300)Spark Program of the state ministry of science and technology of China(2015GA680005).
文摘Loach exhibit conspicuous drilling behaviors in the mud of shallow waters,yet their burrow morphology and the factors affecting this behavior have received little attention.We characterized the burrow morphology and water quality of the pond loach Misgurnus anguillicaudatus in three scenarios:in tanks without plants,tanks with the free-floating plant water hyacinth Eichhornia crassipes,and tanks with water lettuce Pistia stratiotes.Water hyacinth effectively removed water TN,COD,NO3-N and NH4-N,and water lettuce removed water TP and NH4-N.Water hyacinth and water lettuce markedly reduced water turbidity and DO,increased TOC and EC.Water hyacinth purified water more effectively than water lettuce,providing a suitable habitat for loach feeding,living and burrowing.The burrow structures were V-shaped,Y-shaped,inverted L-shaped,or complicated dendritic networks composed of multiple V shapes.The hyacinth treatment was characterized by the greatest burrow volume,length,depth,and structural complexity,but the opening size was reduced by dense root mat coverage.Burrows in the water lettuce treatment were characterized by intermediate volume,length,branches and sinuosity,but they had the largest opening and pit size.The control treatment had a flat bottom with the smallest,shortest burrows.This study indicates that free-floating plants improve habitat suitability and change burrow morphology and may be used to improve loach breeding methods。
基金This work was supported by the Key Project of Chinese Ministry of Education(02080)the Open Fund of State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,The Chinese Academy of Science(2002FB08).
文摘The effects of heavy metals Cd^(2+),Pb^(2+)and Zn^(2+)at 0.05,0.5 and 5.0 mg/L level and their interactions at 0.5 mg/L level on DNA damage in hepatopancreas of loach Misgurnus anguillicaudatus for 1-35 days exposure were examined by single cell gel electrophoresis(SCGE).For each test group,20 loaches with similar body size(5.17-7.99 g;11.79-13.21 cm)were selected and kept in aquaria with dechlori-nated water at(22±1)℃and fed a commercial diet every 48 h.According to the percentage of damaged DNA with tail and its TL/D(tail length to diameter of nucleus)value,the relationship between DNA damage degree and heavy metal dose and exposure time was determined.Results showed that the percentage of damaged DNA and the TL/D value were increased with the prolonged exposure time.The highest percentage(84.85%)of damaged DNA was shown in 5.0 mg/L Zn^(2+)group after 28 days exposure and the biggest TL/D value(2.50)in all treated groups after 35 days exposure.During the first treated week,the damnification of DNA was mainly recognized as the first level,after that time,the third damaged level was mostly observed and the percentage of damaged DNA was beyond 80%.The joint toxic effects among Cd^(2+),Pb^(2+)or Zn^(2+)revealed much complexity,but it generally displayed that the presence of Cd^(2+)could enhance the genotoxicity of Pb^(2+)or Zn^(2+).In conclusion,the results suggestedthattherewasasignificanttime-anddose-depended relationship between the heavy metal and DNA damage in hepatopancreas of loach,and SCGE could represent a useful means to evaluate the genotoxicity of environmental contamination on aquatic organisms.
基金Natural Science Foundation of Zhejiang(LY12C03006),China
文摘This study compared the accumulation of Zn2+and Cu2+in the ovaries and ova of loaches under different concentrations of Zn2+(1.00, 2.50 and 5.00 mg/L respectively) and Cu2+(0.10, 0.25 and 0.50 mg/L respectively). The results showed that both Zn2+and Cu2+accumulated in the ovaries, and that the relationship between accumulation and time was linear over 20 days of exposure. The accumulation of the metals in ovaries was closely related to the concentration of exposure in the solutions(P<0.05), and was obviously affected by the time and doses. However, the Cu2+concentration was significantly higher than Zn2+(P<0.05). The development level of ova in the ovaries also correlated with the concentration and exposure period in the Zn2+and Cu2+solutions.
基金Supported by the Natural Science Foundation of China(31272650)the Natural Science Foundation of Liaoning Province(201102019)
文摘We used microsatellite markers to investigate the genetic parameters of three different polyploidy populations of Misgurnus anguillicaudatus from Honghu City, Hubei Province. Polyacrylamide gel electrophoresis banding patterns of diploid（2n）, triploid（3n） and tetraploid loaches（4n） were analyzed with Pop Gen software. A total of 68 alleles were obtained from seven microsatellite loci and the polymorphism information content（PIC） indices were all above 0.5. The average expected mean heterozygosity values（He） were 0.8420, 0.7186 and 0.8521; the average observed mean heterozygosity values（Ho） were 0.9674, 0.9785 and 0.8928; and the HardyWeinberg P values were 0.3078, 0.3151 and 0.3762, for diploid, triploid and tetraploid individuals, respectively. The results indicated that the three populations were highly polymorphic, with no deviations from Hardy-Weinberg equilibrium observed at all the seven microsatellite loci. This indicated a high level of genetic diversity within the populations. A cluster analysis diagram showed that the shortest genetic distance was between diploid and tetraploid loaches and they shared a close phylogenetic relationship. The triploid and tetraploid individuals had the most distant phylogenetic relationship.
基金Natural Science Foundation of Zhejiang(LY12C03006),China。
文摘This study compared the accumulation of Zn^(2+)and Cu^(2+)in the ovaries and ova of loaches under different concentrations of Zn^(2+)(1.00,2.50 and 5.00 mg/L respectively)and Cu^(2+)(0.10,0.25 and 0.50 mg/L respectively).The results showed that both Zn^(2+)and Cu^(2+)accumulated in the ovaries,and that the relationship between accumulation and time was linear over 20 days of exposure.The accumulation of the metals in ovaries was closely related to the concentration of exposure in the solutions(P<0.05),and was obviously affected by the time and doses.However,the Cu^(2+)concentration was significantly higher than Zn^(2+)(P<0.05).The development level of ova in the ovaries also correlated with the concentration and exposure period in the Zn^(2+)and Cu^(2+)solutions.
文摘A novel oligosaccharide was isolated and purified from the mucus of the loach, Misgurnus anguillicaudatus. It was identified by several qualitative tests and characterized by elementary analysis, UV and IR spectrum. Its average molecular weight (Mw=1539.4) was determined by gel permeation chromatography. The major structural monomers of Misgurnus anguillicaudatus oligosaccharide were identified to be D-galactose and L-fucose by paper chromatography and gas chromatography.
文摘A novel antimicrobial polypeptide was isolated and characterized from loach, Misgurnus anguillicaudatus. The polypeptide, named MAPP, is a single-chain polypeptide with Mw about 9,800 Dalton and pI about 4.78; the N-tag of MAPP was CFGWN. MAPP showed good inhibition against various bacteria including Bacillus subtilis, Escherichia coli and Staphylococcus aureus. MAPP could be used as a lead compound in antibiotics drug discovery.
