中药Ⅰ号对动物生精机能的影响

动物睾丸是产生精子的基地 ,通过制作、观察临时装片、切片 ,检查精液中畸形精子百分率 。

牙周病正畸治疗

目的：探讨正畸治疗牙周病的效果.方法：牙周病是由多种因素引起的牙齿周围组织的慢性疾病,经牙周治疗和正畸治疗,比较前后牙周炎症患者,牙槽骨,牙周袋深浅,牙齿松动度的变化来评价正畸治疗对患有牙周病的错颌畸形的作用.结果：通过12 列个别正常颌矫正结果牙周情况较矫治前有了明显好转.结论：正畸治疗为错颌畸形患者创造了整齐的牙列和功能良好的牙齿,健康的牙周环境有利于菌斑的控制,对牙周病的治疗有明显作用.

残耳软骨细胞诱导脂肪来源干细胞体外软骨形成实验研究

目的探讨残耳软骨细胞能否在体外模拟软骨诱导微环境,促进脂肪来源干细胞(adipose derived stemcells,ADSCs)向软骨分化并形成软骨样组织。方法取外耳再造术中废弃的先天性小耳畸形患者残耳软骨组织与皮下脂肪组织分离培养,分别收集第2代残耳软骨细胞与第3代ADSCs,以3∶7比例混合培养作为实验组(A组),单纯残耳软骨细胞和单纯ADSCs作为对照(分别为B组和C组)。取各组细胞1.0×106个离心培养获得细胞球后,体外培养28 d,大体观察各组细胞球取材时的外观变化,测量湿重;阿利辛蓝比色法检测糖胺多糖(glycosaminoglycan,GAG)含量;RT-PCR检测各组标本的Ⅱ型胶原、蛋白聚糖(Aggrecan)mRNA表达;并行HE、甲苯胺蓝、番红O组织学观察及Ⅱ型胶原免疫组织化学检测。结果体外培养28 d后,A、B组标本形成白色半透明圆盘状组织块,表面光滑,弹性可;C组标本组织块有明显收缩,呈黄色球状,无弹性。A、B组标本湿重及GAG含量显著高于C组(P<0.05);A、B组间差异无统计学意义(t=1.820 3,P=0.068 7;t=1.861 4,P=0.062 7)。RT-PCR检测示A、B组标本均可见Ⅱ型胶原与Aggrecan mRNA条带清晰表达,C组未见明显表达;A、B组Ⅱ型胶原与Aggrecan mRNA表达均显著高于C组(P<0.05),A、B组间差异无统计学意义(t=1.457 6,P=0.144 9;t=1.519 5,P=0.128 6)。组织学观察示:A组与B组细胞球标本形成了大量软骨陷窝样结构,细胞外基质均有不同程度染色;C组细胞球标本组织内主要为纤维性成分,未见软骨陷窝,细胞外基质染色阴性。A、B组可见在软骨陷窝周围有不同程度棕黄色沉淀即Ⅱ型胶原表达,C组未见明显表达;A、B组灰度值显著低于C组(P<0.01),A、B组间差异无统计学意义(t=1.661 5,P=0.097 0)。结论残耳软骨细胞可在体外独立模拟软骨诱导微环境,促进ADSCs软骨定向分化并形成软骨组织。

血尿实验室检测的临床诊断价值

目的:利用红细胞分析仪检测血尿,评价实验室检测的诊断价值。方法:检测86例血尿患者的血尿红细胞平均体积和分布宽度,镜检尿红细胞形态。结果:肾小球性血尿红细胞畸形率为91.11%,非肾小球性血尿红细胞畸形率为29.27%;肾小球性血尿红细胞平均体积为68.36±7.58 fL,非肾源性血尿红细胞平均体积为87.64±7.52 fL;肾小球性血尿红细胞体积分布宽度为25.14±4.37%,非肾源性血尿红细胞体积分布宽度为16.36±2.59%。结论:联合检测患者血尿红细胞形态、平均体积和分布宽度,有一定的诊断参考价值。

Utilization of spent substrates and waste products of mushroom cultivation to produce new crops of Pleurotus ostreatus,Pleurotus eryngii and Agaricus bisporus

Five agro-industrial residues,their corresponding spent mushroom substrates(SMS),commercial fresh and spent mushroom compost of Agaricus(SMC)and Pleurotus ostreatus(SMS GZ)cultivation,Pleurotus and Agaricus waste(stipes/mishappen mushrooms)were combined and re-utilized as novel substrates for the cultivation of P.ostreatus,P.eryngii and A.bisporus mushrooms.SMSs/SMC were used up to 40%(treatment 1 and 2),while 20%of mushroom waste were added in fresh and spent substrates(treatment 3 and 4).The impact of different substrates on mycelial growth rate and biomass production was examined.Then,Pleurotus spp.were cultivated on the most promising substrates and essential cultivation aspects(earliness,total mushroom yield,biological efficiency-BE)and carposomes’quality parameters(weight,morphological characteristics)were evaluated.Laccase and endoglucanase production by Pleurotus species were also determined at 50 and 100%of colonization stages.All species showed their fastest mycelial growth rate(up to 5 mm/day)on substrates consisted of SMC,whereas many combinations of species/substrate enhanced biomass production.The SMS GZ supplementation positively affected laccase activity;in the cultures of P.ostreatus and P.eryngii the highest values were 62,539 and 17,584 U/g d.w.,respectively.On the contrary,small amounts of endoglucanase were produced(0.007 to 0.322 U/g d.w.);the greatest production was recorded for P.ostreatus at full colonization.Regarding fermentation in bags,significant amount of total mushroom yield was produced in all substrates and those with SMS GZ supported the fastest earliness period and the highest BE for both Pleurotus species.BE values ranged from 54 to 133%for P.ostreatus and from 53 to 121%for P.eryngii.Concerning morphological characteristics,mushroom waste addition seemed to affect them positively.The data included in this paper support the effective re-utilization of different types of SMS and mushroom waste for fungal mass and enzymes’production and for new high quality Pleurotus spp.carposomes.