The bombycid moth, Triuncina daii Xing Wang ＆ Zolotuhin, 2015, plays an important role for analyzing the phylogenetic relationships of the family Bombycidae （Lepidoptera： Bombycoidea）. Here we first describe the c...The bombycid moth, Triuncina daii Xing Wang ＆ Zolotuhin, 2015, plays an important role for analyzing the phylogenetic relationships of the family Bombycidae （Lepidoptera： Bombycoidea）. Here we first describe the complete mitochondrial genome （mitogenome） of T. daii, which includes thirteen protein-coding genes （PCGs）, twenty-two transfer RNA genes, two ribosomal RNA genes and an A＋T-rich region, and we find the mitogenome is 15,482 bp in length （GenBank no. KY091643）. The genes order and orientation in the T. daii mitogenome are similar to other sequenced lepidopteran species. Except for cox1, all of the PCGs started with ATN. Twelve PCGs stopped at TAA except for cox1 which stops at a single T. Thirteen PCGs of available species are used to demonstrate the inner phylogenetic relationships of Bombycoidea. The bombycid species form a monophyletic clade with a bootstrap value of 100% and a posterior probability of 1.00.展开更多
Population genetic structure and historical demography of Chinese horseshoe crab (T.tridentatus)along southeast coast of China were inferred from the sequence data of mitochondrial cytochrome c oxidase subunit Ⅰ (COI...Population genetic structure and historical demography of Chinese horseshoe crab (T.tridentatus)along southeast coast of China were inferred from the sequence data of mitochondrial cytochrome c oxidase subunit Ⅰ (COI) fragment.The sequence analysis for 964 bp COI fragment was conducted in 28 individuals collected from five localities:Ninghai in Zhejiang Province,Meizhou and Zhangpu in Fujian Province,Beihai of Guangxi Zhuang Autonomous Region and Danzhou of Hainan Province.Sequence variation was relatively low with a total of seven transitions observed.In all localities,Haplotype H3 was the dominant type observed among eight haplotypes defined previously,and was at the center of radiation in Median-Joining network.The prolonged star-like network suggests a signature of population expansions.The level of diversity was low in total,with haplotype diversity ( Hd) being equal to 0.765 and nucleotide diversity (π) being equal to 0.00118,respectively.The genetic structure analysis revealed the significant genetic difference between Ninghai and Danzhou populations.Both mismatch distribution analysis and Fu's Fs test provided consistent inference of historic population expansion.The low genetic diversity of horseshoe crab observed along China coast indicated that urgent measures should be taken to protect this rare marine animal.展开更多
The aim of this study was to evaluate the genetic variability in bees Apis mellifera from Benin by using mitochondrial DNA （mtDNA） as a molecular marker in their cytochrome c oxidase subunit I and II （COI-COI1） in...The aim of this study was to evaluate the genetic variability in bees Apis mellifera from Benin by using mitochondrial DNA （mtDNA） as a molecular marker in their cytochrome c oxidase subunit I and II （COI-COI1） intergenic region. A total of 304 bee colonies were sampled in 27 municipalities of the cashew growing area of Benin. These samples were analyzed by the cleaved amplified polymorphisms technique for determining the haplotypes of subspecies present in the sampled population. Eight PCR-RFLP profiles of African lineage A were then identified in the 304 samples of bees investigated. Forty-nine percent （49%） of the samples showed the profile of haplotype A1 （subspecies adansonii of Zambia）, 40% of haplotype A4 （subspecies scutellata of South Africa） and 3% of haplotype A 19 （subspecies adansonii of Guinea）. Five other haplotypes of the African branch （A） that had been described in a previous study were also identified： new 1 （2%）, new 2 （2%）, new 3 （1%）, new 4 （2%） and new 5 （1%）. This study showed that A. rnellifera from Benin belonged only to lineage A with the predominance of haplotypes AI and A4. This study will contribute to the development of coherent policies for conservation of local bees in Benin.展开更多
用蛋白酶K消化法提取萧氏松茎象总DNA,并对细胞色素C氧化酶亚基Ⅰ(cytochrome c oxidaseⅠgene,COⅠ)序列分析发现,COⅠ序列4种核苷酸量平均为A=30.2%,C=17.3%,G=15.4%,T=37.0%。将所获得的5个不同地区萧氏松茎象个体COⅠ序列排序后,比...用蛋白酶K消化法提取萧氏松茎象总DNA,并对细胞色素C氧化酶亚基Ⅰ(cytochrome c oxidaseⅠgene,COⅠ)序列分析发现,COⅠ序列4种核苷酸量平均为A=30.2%,C=17.3%,G=15.4%,T=37.0%。将所获得的5个不同地区萧氏松茎象个体COⅠ序列排序后,比较形成了一个含1 209个位点的矩阵,其中1 174个不变位点,35个变异位点,所占比例为2.89%,序列的A+T含量较高。遗传距离、核酸和氨基酸变异及系统发育树分析表明:萧氏松茎象与树皮象属亲缘关系最近,且分子水平上萧氏松茎象与树皮象属之间较之树皮象属内种间无明显差异,对目前的分类地位提出质疑。展开更多
本研究对西北地区三个地方猪种471个样本(藏猪、甘肃黑猪和八眉猪)mt DNA COXⅠ基因构建混合池,利用直接测序技术获得3个猪种COXI基因的序列,进行SNPs筛选及序列分析,并采用MEGA 6.0分析软件基于Kimurk双参数模型应用邻接法构建系统发...本研究对西北地区三个地方猪种471个样本(藏猪、甘肃黑猪和八眉猪)mt DNA COXⅠ基因构建混合池,利用直接测序技术获得3个猪种COXI基因的序列,进行SNPs筛选及序列分析,并采用MEGA 6.0分析软件基于Kimurk双参数模型应用邻接法构建系统发生树。结果表明,3个猪种mt DNA COXI基因序列仅存在5个突变位点,且无相同突变位点,多态性较贫乏。系统进化树和遗传距离分析显示,八眉猪、藏猪、黑猪及野猪聚为一支,再与杜洛克、欧洲伯克希尔猪及大白猪聚为一支,而后与同属为偶蹄目的牛、绵羊聚为一支。西北猪种应有共同的母系祖先,品种内母系遗传多样性较贫乏,外来猪种引入我国后主要是用作父本以提高生产速度和瘦肉率等,对本地猪种未有母系贡献。本研究在一定程度上为我国地方猪品种的有效保护和合理利用提供理论依据。展开更多
本研究对三个国外猪种216个样本(大白猪、长白猪和杜洛克)mt DNA COXⅠ基因构建混合池,利用直接测序技术获得3个猪种COXⅠ基因的序列,进行SNPs筛选及序列分析,并采用MEGA 6.0分析软件基于Kimurk双参数模型应用邻接法构建系统发生树。结...本研究对三个国外猪种216个样本(大白猪、长白猪和杜洛克)mt DNA COXⅠ基因构建混合池,利用直接测序技术获得3个猪种COXⅠ基因的序列,进行SNPs筛选及序列分析,并采用MEGA 6.0分析软件基于Kimurk双参数模型应用邻接法构建系统发生树。结果表明,3个猪种mt DNA COXⅠ基因序列共存在16个突变位点,且有3个突变位点为3个猪种共有。长白猪与杜洛克猪的多态性较丰富,含有13个相同位点的突变,核苷酸的转换数(si)和颠换数(sv)的比值(R)分别为12和15,序列替换远未达到饱和。系统进化树和遗传距离分析显示长白猪及杜洛克亲缘关系较近,先聚为一支,再与大白猪、及野猪聚为一支。大白猪与中国东北地区野猪聚为一支,这可能与大白猪在培育过程中亲本遗传背景较复杂有关。本研究在一定程度上为了解大白猪的育成史及合理利用国外猪种提供了理论依据。展开更多
采用PCR技术扩增6个地理种群桑天牛的线粒体细胞色素氧化酶Ⅰ（mt DNA COⅠ）基因序列,基于mt DNA COⅠ基因序列变异探讨不同地理分布桑天牛种群间的遗传距离、系统发育以及遗传分化程度。对6个地理种群的33个桑天牛样本的mt DNA COⅠ...采用PCR技术扩增6个地理种群桑天牛的线粒体细胞色素氧化酶Ⅰ（mt DNA COⅠ）基因序列,基于mt DNA COⅠ基因序列变异探讨不同地理分布桑天牛种群间的遗传距离、系统发育以及遗传分化程度。对6个地理种群的33个桑天牛样本的mt DNA COⅠ基因扩增产物的测序结果表明共有19个位点发生变异,占序列总长的4.0%,其中来自河南省济源的桑天牛有12个变异位点,来自浙江省的桑天牛有9个变异位点,来自河北省4个地区的桑天牛的变异位点较少;河北省的4个地理种群的遗传距离较小,在0.001 3-0.002 6之间,河北、河南、浙江种群间的遗传距离较大,其中河南种群与河北种群的遗传距离又远远大于浙江种群与河北种群的遗传距离,后2大地理种群分布区域的海拔更接近。利用MEGA4.1软件构建的NJ系统进化树显示,6个地理种群形成河北、河南和浙江3大地理分布格局;从遗传分化程度上来看,桑天牛种群的3大地理分布格局不存在共享的单元型,遗传分化系数（FST）在0.804 1-0.892 2范围内,遗传分化程度较高。综合分析认为,不同地理种群桑天牛存在分化的原因除地理分布外,更重要的是生态条件的差异。展开更多
基金supported by the National Natural Science Foundation of China(31100482,31411140034)the Scientific Research Fund of Hunan Provincial Education Department(16B119)
文摘The bombycid moth, Triuncina daii Xing Wang ＆ Zolotuhin, 2015, plays an important role for analyzing the phylogenetic relationships of the family Bombycidae （Lepidoptera： Bombycoidea）. Here we first describe the complete mitochondrial genome （mitogenome） of T. daii, which includes thirteen protein-coding genes （PCGs）, twenty-two transfer RNA genes, two ribosomal RNA genes and an A＋T-rich region, and we find the mitogenome is 15,482 bp in length （GenBank no. KY091643）. The genes order and orientation in the T. daii mitogenome are similar to other sequenced lepidopteran species. Except for cox1, all of the PCGs started with ATN. Twelve PCGs stopped at TAA except for cox1 which stops at a single T. Thirteen PCGs of available species are used to demonstrate the inner phylogenetic relationships of Bombycoidea. The bombycid species form a monophyletic clade with a bootstrap value of 100% and a posterior probability of 1.00.
基金F5 Subject from Science and Technology Department of Fujian Province under contract No. 2008F5038the Open Foundation from Ocean Fishery Science and Technology in the Most Important Subjects of Zhejiang under contract No.20100210
文摘Population genetic structure and historical demography of Chinese horseshoe crab (T.tridentatus)along southeast coast of China were inferred from the sequence data of mitochondrial cytochrome c oxidase subunit Ⅰ (COI) fragment.The sequence analysis for 964 bp COI fragment was conducted in 28 individuals collected from five localities:Ninghai in Zhejiang Province,Meizhou and Zhangpu in Fujian Province,Beihai of Guangxi Zhuang Autonomous Region and Danzhou of Hainan Province.Sequence variation was relatively low with a total of seven transitions observed.In all localities,Haplotype H3 was the dominant type observed among eight haplotypes defined previously,and was at the center of radiation in Median-Joining network.The prolonged star-like network suggests a signature of population expansions.The level of diversity was low in total,with haplotype diversity ( Hd) being equal to 0.765 and nucleotide diversity (π) being equal to 0.00118,respectively.The genetic structure analysis revealed the significant genetic difference between Ninghai and Danzhou populations.Both mismatch distribution analysis and Fu's Fs test provided consistent inference of historic population expansion.The low genetic diversity of horseshoe crab observed along China coast indicated that urgent measures should be taken to protect this rare marine animal.
文摘The aim of this study was to evaluate the genetic variability in bees Apis mellifera from Benin by using mitochondrial DNA （mtDNA） as a molecular marker in their cytochrome c oxidase subunit I and II （COI-COI1） intergenic region. A total of 304 bee colonies were sampled in 27 municipalities of the cashew growing area of Benin. These samples were analyzed by the cleaved amplified polymorphisms technique for determining the haplotypes of subspecies present in the sampled population. Eight PCR-RFLP profiles of African lineage A were then identified in the 304 samples of bees investigated. Forty-nine percent （49%） of the samples showed the profile of haplotype A1 （subspecies adansonii of Zambia）, 40% of haplotype A4 （subspecies scutellata of South Africa） and 3% of haplotype A 19 （subspecies adansonii of Guinea）. Five other haplotypes of the African branch （A） that had been described in a previous study were also identified： new 1 （2%）, new 2 （2%）, new 3 （1%）, new 4 （2%） and new 5 （1%）. This study showed that A. rnellifera from Benin belonged only to lineage A with the predominance of haplotypes AI and A4. This study will contribute to the development of coherent policies for conservation of local bees in Benin.
文摘用蛋白酶K消化法提取萧氏松茎象总DNA,并对细胞色素C氧化酶亚基Ⅰ(cytochrome c oxidaseⅠgene,COⅠ)序列分析发现,COⅠ序列4种核苷酸量平均为A=30.2%,C=17.3%,G=15.4%,T=37.0%。将所获得的5个不同地区萧氏松茎象个体COⅠ序列排序后,比较形成了一个含1 209个位点的矩阵,其中1 174个不变位点,35个变异位点,所占比例为2.89%,序列的A+T含量较高。遗传距离、核酸和氨基酸变异及系统发育树分析表明:萧氏松茎象与树皮象属亲缘关系最近,且分子水平上萧氏松茎象与树皮象属之间较之树皮象属内种间无明显差异,对目前的分类地位提出质疑。
文摘本研究对西北地区三个地方猪种471个样本(藏猪、甘肃黑猪和八眉猪)mt DNA COXⅠ基因构建混合池,利用直接测序技术获得3个猪种COXI基因的序列,进行SNPs筛选及序列分析,并采用MEGA 6.0分析软件基于Kimurk双参数模型应用邻接法构建系统发生树。结果表明,3个猪种mt DNA COXI基因序列仅存在5个突变位点,且无相同突变位点,多态性较贫乏。系统进化树和遗传距离分析显示,八眉猪、藏猪、黑猪及野猪聚为一支,再与杜洛克、欧洲伯克希尔猪及大白猪聚为一支,而后与同属为偶蹄目的牛、绵羊聚为一支。西北猪种应有共同的母系祖先,品种内母系遗传多样性较贫乏,外来猪种引入我国后主要是用作父本以提高生产速度和瘦肉率等,对本地猪种未有母系贡献。本研究在一定程度上为我国地方猪品种的有效保护和合理利用提供理论依据。
文摘本研究对三个国外猪种216个样本(大白猪、长白猪和杜洛克)mt DNA COXⅠ基因构建混合池,利用直接测序技术获得3个猪种COXⅠ基因的序列,进行SNPs筛选及序列分析,并采用MEGA 6.0分析软件基于Kimurk双参数模型应用邻接法构建系统发生树。结果表明,3个猪种mt DNA COXⅠ基因序列共存在16个突变位点,且有3个突变位点为3个猪种共有。长白猪与杜洛克猪的多态性较丰富,含有13个相同位点的突变,核苷酸的转换数(si)和颠换数(sv)的比值(R)分别为12和15,序列替换远未达到饱和。系统进化树和遗传距离分析显示长白猪及杜洛克亲缘关系较近,先聚为一支,再与大白猪、及野猪聚为一支。大白猪与中国东北地区野猪聚为一支,这可能与大白猪在培育过程中亲本遗传背景较复杂有关。本研究在一定程度上为了解大白猪的育成史及合理利用国外猪种提供了理论依据。
文摘采用PCR技术扩增6个地理种群桑天牛的线粒体细胞色素氧化酶Ⅰ（mt DNA COⅠ）基因序列,基于mt DNA COⅠ基因序列变异探讨不同地理分布桑天牛种群间的遗传距离、系统发育以及遗传分化程度。对6个地理种群的33个桑天牛样本的mt DNA COⅠ基因扩增产物的测序结果表明共有19个位点发生变异,占序列总长的4.0%,其中来自河南省济源的桑天牛有12个变异位点,来自浙江省的桑天牛有9个变异位点,来自河北省4个地区的桑天牛的变异位点较少;河北省的4个地理种群的遗传距离较小,在0.001 3-0.002 6之间,河北、河南、浙江种群间的遗传距离较大,其中河南种群与河北种群的遗传距离又远远大于浙江种群与河北种群的遗传距离,后2大地理种群分布区域的海拔更接近。利用MEGA4.1软件构建的NJ系统进化树显示,6个地理种群形成河北、河南和浙江3大地理分布格局;从遗传分化程度上来看,桑天牛种群的3大地理分布格局不存在共享的单元型,遗传分化系数（FST）在0.804 1-0.892 2范围内,遗传分化程度较高。综合分析认为,不同地理种群桑天牛存在分化的原因除地理分布外,更重要的是生态条件的差异。
