[Objectives]The paper was to establish a TLC identification method for Ensete wilsonii.[Methods]Usingβ-sitosterol as the reference,the effects of preparation methods of test solutions,developing solvents,developing d...[Objectives]The paper was to establish a TLC identification method for Ensete wilsonii.[Methods]Usingβ-sitosterol as the reference,the effects of preparation methods of test solutions,developing solvents,developing distances and color developing agents on TLC analysis were investigated,and the best TLC conditions for E.wilsonii were determined.[Results]The test solution prepared with 90%methanol solvent was dotted on TLC silica gel G plate,and developed with dichloromethane-toluene-methanol=10:5:1.5 as the developing solvent.Then the plate was sprayed with 10%sulfuric acid ethanol solution,and dried with hot blast for color development.Finally,the plate was examined under an ultraviolet lamp at 365 nm.The TLC results of E.wilsonii obtained showed good separation and color development effect,and the spots were clear and characteristic.[Conclusions]This method is safe,specific,and easy to operate,and can be used as a TLC identification method for E.wilsonii.展开更多
[Objectives]To establish a TLC and content determination method of Pileostegia tomentellal,with umbelliferone as the indicator component.[Methods]TLC identification was performed by silica gel G thin layer plate with ...[Objectives]To establish a TLC and content determination method of Pileostegia tomentellal,with umbelliferone as the indicator component.[Methods]TLC identification was performed by silica gel G thin layer plate with n-hexane-ethyl acetate(4:3)as the developing agent,and the plate was examined by UV lamp(365 nm).The umbelliferone content was determined by HPLC:Inertsil ODS-3 C 18 column(4.60 mm×250 mm,5μm);mobile phase acetonitrile-0.2%phosphoric acid gradient elution;detection wavelength 320 nm,flow rate 1.0 mL/min,column temperature 30℃,injection volume 10μL.[Results]The chromatogram of P.tomentellal showed the same color spot in the same position as that of reference medicinal material,and the spot was clear with good specificity.Umbelliferone showed a good linear relationship when the injection volume was 2.63-131.27μg/mL(R^(2)=0.9997).The average recovery of umbelliferone in the low,middle and high adding groups of P.tomentellal was 99.57%and the RSD was 2.15%.[Conclusions]The method can effectively identify Yao medicine P.tomentellal and accurately determine the content of umbelliferone in medicinal materials,which will provide a scientific basis for the development and utilization of medicinal resources of Yao medicine P.tomentellal.展开更多
[Objectives]This study was conducted to establish TLC methods for Scutellariae Radix,Peucedani Radix and Puerariae Radix in Chaige Changyuan Mixture.[Methods]The preparation methods of test solutions,the development c...[Objectives]This study was conducted to establish TLC methods for Scutellariae Radix,Peucedani Radix and Puerariae Radix in Chaige Changyuan Mixture.[Methods]The preparation methods of test solutions,the development conditions,the color development inspection method and other factors were investigated for thin-layer chromatography.The three main medicines of Scutellariae Radix,Peucedani Radix and Puerariae Radix were qualitatively identified.[Results]The TLC spots of Scutellariae Radix,Peucedani Radix and Puerariae Radix could be identified well by TLC,and corresponding negative control had no interference.[Conclusions]The method is simple,available and reproducible,and can be used for the quality control of Chaige Changyuan Mixture.展开更多
Objective Body fluid mixtures are complex biological samples that frequently occur in crime scenes,and can provide important clues for criminal case analysis.DNA methylation assay has been applied in the identificatio...Objective Body fluid mixtures are complex biological samples that frequently occur in crime scenes,and can provide important clues for criminal case analysis.DNA methylation assay has been applied in the identification of human body fluids,and has exhibited excellent performance in predicting single-source body fluids.The present study aims to develop a methylation SNaPshot multiplex system for body fluid identification,and accurately predict the mixture samples.In addition,the value of DNA methylation in the prediction of body fluid mixtures was further explored.Methods In the present study,420 samples of body fluid mixtures and 250 samples of single body fluids were tested using an optimized multiplex methylation system.Each kind of body fluid sample presented the specific methylation profiles of the 10 markers.Results Significant differences in methylation levels were observed between the mixtures and single body fluids.For all kinds of mixtures,the Spearman’s correlation analysis revealed a significantly strong correlation between the methylation levels and component proportions(1:20,1:10,1:5,1:1,5:1,10:1 and 20:1).Two random forest classification models were trained for the prediction of mixture types and the prediction of the mixture proportion of 2 components,based on the methylation levels of 10 markers.For the mixture prediction,Model-1 presented outstanding prediction accuracy,which reached up to 99.3%in 427 training samples,and had a remarkable accuracy of 100%in 243 independent test samples.For the mixture proportion prediction,Model-2 demonstrated an excellent accuracy of 98.8%in 252 training samples,and 98.2%in 168 independent test samples.The total prediction accuracy reached 99.3%for body fluid mixtures and 98.6%for the mixture proportions.Conclusion These results indicate the excellent capability and powerful value of the multiplex methylation system in the identification of forensic body fluid mixtures.展开更多
[Objectives] The aim of this study was to screen out the TLC conditions of matrine in Jin Weng Zhi Li Granule. [Methods]Referring to the method of Chinese Veterinary Pharmacopoeia,the composition,chromogenic agent and...[Objectives] The aim of this study was to screen out the TLC conditions of matrine in Jin Weng Zhi Li Granule. [Methods]Referring to the method of Chinese Veterinary Pharmacopoeia,the composition,chromogenic agent and expansion conditions of the developer were optimized,and matrine was qualitatively identified by TLC. [Results] After optimizing TLC conditions of Jin Weng Zhi Li Granule,that is,the thin layer plate was pre-equilibrated in the expansion cylinder for 5 min after spotting,the separation effect of TLC of test samples was favorable. The spot color was clear,reproducibility was good,precision was high,specificity was better. [Conclusions] The method was of good feasibility,reproducibility and specificity,and could be used for qualitative detection of matrine in Jin Weng Zhi Li Granule dampnessheat and diarrhea disease.展开更多
Fingerprints of two varieties of rice and their mixtures were investigated by a nonlinear chemical reaction system consisting of rice components,sodium bromate,manganese sulfate,sulfuric acid and acetone.The variety o...Fingerprints of two varieties of rice and their mixtures were investigated by a nonlinear chemical reaction system consisting of rice components,sodium bromate,manganese sulfate,sulfuric acid and acetone.The variety of rice was identified by the visual characteristic of fingerprint and system similarity pattern recognition,and the content of each variety of rice in the mixture was determined by the quantitative information of fingerprint.The results show that nonlinear chemical analysis may be used to exactly identify the variety of pure rice and to accurately determine the content of each variety of rice in the mixture,indicating the method is simple and convenient.展开更多
[ Objective ] We aimed to establish the identification method of gardenoside and sarsasapogenin in Qingwen baidu granules. [ Method] With ethyl ace- tate-acetone-formic acid-water (10:7:2:0.5) as the developer an...[ Objective ] We aimed to establish the identification method of gardenoside and sarsasapogenin in Qingwen baidu granules. [ Method] With ethyl ace- tate-acetone-formic acid-water (10:7:2:0.5) as the developer and 10% sulfuric acid ethanol solution as the chromogenic reagent, the samples were heated at 100 ~C until the spots were clearly visible, and the existence of gardenoside was checked under natural light. With toluene-acetone(9:l ) as the developer and 5% vanillin sulfuric acid solution as the chromogenic reagent, the samples were heated at 105 ~C until the spots were clearly visible, and the existence of sarsasapogenin was checked under natural light. [Result] Qingwen baidu granules had the same spots with gardenoside and sarsasapogenin at the same Rfvalue under natural light. [ Conclusion] A TLC method detecting gardenoside and sarsasapogenin in Qingwen baidu granules was established, which had good specificity and repeatability, suitable for rapid detection of gardenoside and sarsasapogenin.展开更多
[ Objective] The paper was to establish the identification method of Coptis chinensis and Forsythia suspensa in Qingwen baidu granules. [ Method ] With benzene-ethyl acetate-methanol-isopropanol-cancentrated ammonium ...[ Objective] The paper was to establish the identification method of Coptis chinensis and Forsythia suspensa in Qingwen baidu granules. [ Method ] With benzene-ethyl acetate-methanol-isopropanol-cancentrated ammonium liquid ( 12:6:3:3:1 ) as the developer, the samples were outspread in the cylinder with sat- urated ammonia steam by thin layer chromatography (TLC), and the existence of C. chinensis and berberine hydrochloride was checked under UV lamp. With tri- chloromethane-methanol (5:1) as the developer and 10% sulfuric acid ethanol solution as the chromogenic reagent, the samples were heated at 105 ℃ for 5 min to check the existence of F. suspense under natural light. [Result] Qingwen baidu granules had the same spots with C. chinensis control and berberine hydrochloride at the same Rf value under 365 nm UV lamp; Qingweu baidu granules had the same spots with F. suspensa control at the same Rfvalue under natural light. [ Condusion] A TLC method detecting C. chinensis and F. suspensa in Qingwen baidu granules was established, which had good specificity and repeatability, suitable for rapid detection of C. chinensis and F. suspensa. It could be well applied to control the quality of Qingwen baidu granule.展开更多
[Objectives]To study the quality standard of Chinese drug in compound Qianglinaogingsu Tablets(Fructus Schisandrae Chinensis and Coru Cervi Pantotrichum).[Methods]The thin layer chromatography(TLC)differentiation meth...[Objectives]To study the quality standard of Chinese drug in compound Qianglinaogingsu Tablets(Fructus Schisandrae Chinensis and Coru Cervi Pantotrichum).[Methods]The thin layer chromatography(TLC)differentiation method was established for Fructus Schisandrae Chinensis and Cornu Cervi Pantotrichum.[Results]TLC spots developed were fairly clear,and the blank test showed no interference.[Conclusions]The method is fast,simple and can be used for quality control of Qianglinaoqingsu Tablets.展开更多
[Objectives]Taking chlorogenic acid as index component,TLC and content determination method of Laggerae Alatae Herba were established.[Methods]TLC identification used silica gel G thin-layer plate,and butyl acetate∶f...[Objectives]Taking chlorogenic acid as index component,TLC and content determination method of Laggerae Alatae Herba were established.[Methods]TLC identification used silica gel G thin-layer plate,and butyl acetate∶formic acid∶water(7∶2.5∶2.5)was taken as developing agent,and it was inspected under ultraviolet lamp(365 nm).The content was determined by chromatographic column Inertsil ODS-3 C_(18)(4.60 mm×250 mm,5μm).Mobile phase:methanol-0.1%phosphoric acid(28∶72);detection wavelength:329 nm;flow speed:1.0 mL/min;column temperature:25℃;injection volume:10μL.[Results]Chlorogenic acid can be detected by TLC,with clear spots and good specificity.When injection volume of chlorogenic acid was between 0.099 and 0.990μg(R^(2)=0.9999),there was good linear relationship.In low,medium and high sample adding groups of Laggerae Alatae Herba,average recovery rates of chlorogenic acids were 98.80%(RSD=2.09%),98.24%(RSD=1.96%)and 99.65%(RSD=2.15%).[Conclusions]The method could effectively identify medicinal material Laggerae Alatae Herba,and accurately measure the content of chlorogenic acid in Laggerae Alatae Herba,thereby providing a scientific basis for developing and using medicinal resources of Laggerae Alatae Herba.展开更多
This letter proposes an effective and robust speech feature extraction method based on statistical analysis of Pitch Frequency Distributions (PFD) for speaker identification. Compared with the conventional cepstrum, P...This letter proposes an effective and robust speech feature extraction method based on statistical analysis of Pitch Frequency Distributions (PFD) for speaker identification. Compared with the conventional cepstrum, PFD is relatively insensitive to Additive White Gaussian Noise (AWGN), but it does not show good performance for speaker identification, even if under clean environments. To compensate this shortcoming, PFD and conventional cepstrum are combined to make the ultimate decision, instead of simply taking one kind of features into account.Experimental results indicate that the hybrid approach can give outstanding improvement for text-independent speaker identification under noisy environments corrupted by AWGN.展开更多
In this paper, an efficient model of palmprint identification is presented based on subspace density estimation using Gaussian Mixture Model (GMM). While a few training samples are available for each person, we use in...In this paper, an efficient model of palmprint identification is presented based on subspace density estimation using Gaussian Mixture Model (GMM). While a few training samples are available for each person, we use intrapersonal palmprint deformations to train the global GMM instead of modeling GMMs for every class. To reduce the dimension of such variations while preserving density function of sample space, Principle Component Analysis (PCA) is used to find the principle differences and form the Intrapersonal Deformation Subspace (IDS). After training GMM using Expectation Maximization (EM) algorithm in IDS, a maximum likelihood strategy is carried out to identify a person. Experimental results demonstrate the advantage of our method compared with traditional PCA method and single Gaussian strategy.展开更多
Anatomical and TLC study of three Chinese peonies were conducted to make a comparative analysis. Peonies (Paeonia suffruticosa (tree peony), Paeonia lactiflora (Chinese peony) and Paeonia veitchii (Chinese peony)) are...Anatomical and TLC study of three Chinese peonies were conducted to make a comparative analysis. Peonies (Paeonia suffruticosa (tree peony), Paeonia lactiflora (Chinese peony) and Paeonia veitchii (Chinese peony)) are traditionally used on the Qinghai-Tibet Plateau in China. Recent studies have shown that the peonies have different pharmacological activities and clinical applications. To distinguish these three species of peonies and ensure the safety and effectiveness in their use, the microscopic characteristics and chromatographic profile of their roots and the corresponding powder were studied. Plant materials sectioned and stained and the raw powder were studied with an optical microscope using standard techniques in microscopy. The results of microscopic features and TLC were described and illustrated. The three species have different microscopic characteristics and TLC profiles, which allow us to distinguish them. In fact, with the help of features semi-quantitative and qualitative, an identification key was developed in our work and illustrated with photos and a table. The aim of our work was to show that the optical microscopy and related techniques provide a achievable practicality, which can be applied without ambiguity to the authentication of species peonies.展开更多
[Objectives]To study the original plants,characters,tissue structure,powder characteristics and thin-layer chromatography(TLC)characteristics of Cardamine tangutorum and Cardamine macrophylla as Tibetan and Qiang edib...[Objectives]To study the original plants,characters,tissue structure,powder characteristics and thin-layer chromatography(TLC)characteristics of Cardamine tangutorum and Cardamine macrophylla as Tibetan and Qiang edible and medicinal herbs,and to provide the basis for the identification of C.tangutorum and C.macrophylla.[Methods]The identification of C.macrophyll and C.tangutorum was carried out by original plant identification,character identification,microscopic identification and TLC identification.[Results]C.tangutorum and C.macrophylla can be distinguished according to the shape of rhizome and stem,the difference of stem leaves and leaflets,and the difference of flower color;there is no obvious difference between the characteristics of the shape and the powder;the thin layer chromatography shows that in the thin layer chromatography of C.tangutorum and C.macrophylla,spots with the same color are shown on the corresponding positions of the ground part and the reference substance quercetin;the underground part and the position corresponding to the reference substanceβ-sitosterol all show the same color spots.[Conclusions]This study provides a reference for the identification of C.tangutorum and C.macrophylla.展开更多
[Objectives]To explore morphological identification,macroscopical identification,microscopic identification,thin layer chromatography(TLC)identification of Tibetan medical material Dracocephalum tanguticum Maxim.,and ...[Objectives]To explore morphological identification,macroscopical identification,microscopic identification,thin layer chromatography(TLC)identification of Tibetan medical material Dracocephalum tanguticum Maxim.,and provide experimental data for its identification and application.[Methods]The Tibetan medical material was identified by means of original plant,characters,powder,paraffin section and thin layer chromatography(TLC).[Results]Tibetan medical material D.tanguticum Maxim.was obviously distinguished in character identification and microscopic identification,and the TLC method was simple and feasible.[Conclusions]The results will provide the source work foundation for the formulation of the quality standard of Sichuan Province(draft)for Tibetan medicinal material"D.tanguticum Maxim."and the development of pharmaceutical preparations for medical institutions.展开更多
基金Supported by Innovation Project of Guangxi Graduate Education of GXUCM(YCSY2022012)High-level Innovation Team and Outstanding Scholars Program of Universities and Colleges in Guangxi(GJR[2014]07)Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica(20-065-38).
文摘[Objectives]The paper was to establish a TLC identification method for Ensete wilsonii.[Methods]Usingβ-sitosterol as the reference,the effects of preparation methods of test solutions,developing solvents,developing distances and color developing agents on TLC analysis were investigated,and the best TLC conditions for E.wilsonii were determined.[Results]The test solution prepared with 90%methanol solvent was dotted on TLC silica gel G plate,and developed with dichloromethane-toluene-methanol=10:5:1.5 as the developing solvent.Then the plate was sprayed with 10%sulfuric acid ethanol solution,and dried with hot blast for color development.Finally,the plate was examined under an ultraviolet lamp at 365 nm.The TLC results of E.wilsonii obtained showed good separation and color development effect,and the spots were clear and characteristic.[Conclusions]This method is safe,specific,and easy to operate,and can be used as a TLC identification method for E.wilsonii.
基金Supported by Self-funded Research Project of Administration of Traditional Chinese Medicine of Guangxi Zhuang Autonomous Region(GXZYA20220171)Young and Middle-aged Teachers Research Basic Ability Improvement Project of Colleges and Universities in Guangxi(2022KY0307)+5 种基金General Project of Guangxi University of Chinese Medicine(2022MS038)"Qingmiao Project"Talent Cultivation Program of Guangxi International Zhuang Medical Hospital(2022001)Key Project of Guangxi International Zhuang Medical Hospital(GZ2021010)High-level TCM Key Discipline(Zhuang Medical Science)Construction Project of State Administration of Traditional Chinese Medicine(zyyzdxk-2023165)Key Research and Development Project of Guangxi Provincial Department of Science and Technology(GK AB21196057)High-level Talent Cultivation Innovation Team Funding Project of Guangxi University of Chinese Medicine(2022A008).
文摘[Objectives]To establish a TLC and content determination method of Pileostegia tomentellal,with umbelliferone as the indicator component.[Methods]TLC identification was performed by silica gel G thin layer plate with n-hexane-ethyl acetate(4:3)as the developing agent,and the plate was examined by UV lamp(365 nm).The umbelliferone content was determined by HPLC:Inertsil ODS-3 C 18 column(4.60 mm×250 mm,5μm);mobile phase acetonitrile-0.2%phosphoric acid gradient elution;detection wavelength 320 nm,flow rate 1.0 mL/min,column temperature 30℃,injection volume 10μL.[Results]The chromatogram of P.tomentellal showed the same color spot in the same position as that of reference medicinal material,and the spot was clear with good specificity.Umbelliferone showed a good linear relationship when the injection volume was 2.63-131.27μg/mL(R^(2)=0.9997).The average recovery of umbelliferone in the low,middle and high adding groups of P.tomentellal was 99.57%and the RSD was 2.15%.[Conclusions]The method can effectively identify Yao medicine P.tomentellal and accurately determine the content of umbelliferone in medicinal materials,which will provide a scientific basis for the development and utilization of medicinal resources of Yao medicine P.tomentellal.
基金Supported by First-class Discipline Construction Project in Guizhou Province(Chinese Pharmacy)(GNYL[2017]008):Subsidiary Subject(GNYL[2017]008-7)Scientific Research Project of Guizhou University of Traditional Chinese Medicine(3040-04020001406)。
文摘[Objectives]This study was conducted to establish TLC methods for Scutellariae Radix,Peucedani Radix and Puerariae Radix in Chaige Changyuan Mixture.[Methods]The preparation methods of test solutions,the development conditions,the color development inspection method and other factors were investigated for thin-layer chromatography.The three main medicines of Scutellariae Radix,Peucedani Radix and Puerariae Radix were qualitatively identified.[Results]The TLC spots of Scutellariae Radix,Peucedani Radix and Puerariae Radix could be identified well by TLC,and corresponding negative control had no interference.[Conclusions]The method is simple,available and reproducible,and can be used for the quality control of Chaige Changyuan Mixture.
基金supported by the grants from the Natural Science Foundation of Hubei Province(No.2020CFB780)the Fundamental Research Funds for the Central Universities(No.2017KFYXJJ020).
文摘Objective Body fluid mixtures are complex biological samples that frequently occur in crime scenes,and can provide important clues for criminal case analysis.DNA methylation assay has been applied in the identification of human body fluids,and has exhibited excellent performance in predicting single-source body fluids.The present study aims to develop a methylation SNaPshot multiplex system for body fluid identification,and accurately predict the mixture samples.In addition,the value of DNA methylation in the prediction of body fluid mixtures was further explored.Methods In the present study,420 samples of body fluid mixtures and 250 samples of single body fluids were tested using an optimized multiplex methylation system.Each kind of body fluid sample presented the specific methylation profiles of the 10 markers.Results Significant differences in methylation levels were observed between the mixtures and single body fluids.For all kinds of mixtures,the Spearman’s correlation analysis revealed a significantly strong correlation between the methylation levels and component proportions(1:20,1:10,1:5,1:1,5:1,10:1 and 20:1).Two random forest classification models were trained for the prediction of mixture types and the prediction of the mixture proportion of 2 components,based on the methylation levels of 10 markers.For the mixture prediction,Model-1 presented outstanding prediction accuracy,which reached up to 99.3%in 427 training samples,and had a remarkable accuracy of 100%in 243 independent test samples.For the mixture proportion prediction,Model-2 demonstrated an excellent accuracy of 98.8%in 252 training samples,and 98.2%in 168 independent test samples.The total prediction accuracy reached 99.3%for body fluid mixtures and 98.6%for the mixture proportions.Conclusion These results indicate the excellent capability and powerful value of the multiplex methylation system in the identification of forensic body fluid mixtures.
基金Supported by Special Project of National Pubic Benefit Industry(Agriculture)(201303040-05)Special Project of Science and Technology Groundwork(2013FYF110600)
文摘[Objectives] The aim of this study was to screen out the TLC conditions of matrine in Jin Weng Zhi Li Granule. [Methods]Referring to the method of Chinese Veterinary Pharmacopoeia,the composition,chromogenic agent and expansion conditions of the developer were optimized,and matrine was qualitatively identified by TLC. [Results] After optimizing TLC conditions of Jin Weng Zhi Li Granule,that is,the thin layer plate was pre-equilibrated in the expansion cylinder for 5 min after spotting,the separation effect of TLC of test samples was favorable. The spot color was clear,reproducibility was good,precision was high,specificity was better. [Conclusions] The method was of good feasibility,reproducibility and specificity,and could be used for qualitative detection of matrine in Jin Weng Zhi Li Granule dampnessheat and diarrhea disease.
基金Project(61533021) supported by the National Natural Science Foundation of China
文摘Fingerprints of two varieties of rice and their mixtures were investigated by a nonlinear chemical reaction system consisting of rice components,sodium bromate,manganese sulfate,sulfuric acid and acetone.The variety of rice was identified by the visual characteristic of fingerprint and system similarity pattern recognition,and the content of each variety of rice in the mixture was determined by the quantitative information of fingerprint.The results show that nonlinear chemical analysis may be used to exactly identify the variety of pure rice and to accurately determine the content of each variety of rice in the mixture,indicating the method is simple and convenient.
基金Supported by Technical Innovation Project of Shandong Province(201210916001)
文摘[ Objective ] We aimed to establish the identification method of gardenoside and sarsasapogenin in Qingwen baidu granules. [ Method] With ethyl ace- tate-acetone-formic acid-water (10:7:2:0.5) as the developer and 10% sulfuric acid ethanol solution as the chromogenic reagent, the samples were heated at 100 ~C until the spots were clearly visible, and the existence of gardenoside was checked under natural light. With toluene-acetone(9:l ) as the developer and 5% vanillin sulfuric acid solution as the chromogenic reagent, the samples were heated at 105 ~C until the spots were clearly visible, and the existence of sarsasapogenin was checked under natural light. [Result] Qingwen baidu granules had the same spots with gardenoside and sarsasapogenin at the same Rfvalue under natural light. [ Conclusion] A TLC method detecting gardenoside and sarsasapogenin in Qingwen baidu granules was established, which had good specificity and repeatability, suitable for rapid detection of gardenoside and sarsasapogenin.
基金Supported by Technical Innovation Project of Shandong Province(201210916001)
文摘[ Objective] The paper was to establish the identification method of Coptis chinensis and Forsythia suspensa in Qingwen baidu granules. [ Method ] With benzene-ethyl acetate-methanol-isopropanol-cancentrated ammonium liquid ( 12:6:3:3:1 ) as the developer, the samples were outspread in the cylinder with sat- urated ammonia steam by thin layer chromatography (TLC), and the existence of C. chinensis and berberine hydrochloride was checked under UV lamp. With tri- chloromethane-methanol (5:1) as the developer and 10% sulfuric acid ethanol solution as the chromogenic reagent, the samples were heated at 105 ℃ for 5 min to check the existence of F. suspense under natural light. [Result] Qingwen baidu granules had the same spots with C. chinensis control and berberine hydrochloride at the same Rf value under 365 nm UV lamp; Qingweu baidu granules had the same spots with F. suspensa control at the same Rfvalue under natural light. [ Condusion] A TLC method detecting C. chinensis and F. suspensa in Qingwen baidu granules was established, which had good specificity and repeatability, suitable for rapid detection of C. chinensis and F. suspensa. It could be well applied to control the quality of Qingwen baidu granule.
基金Chinese Pharmacopoeia Commission Drug Standardization Research Project(2019Z061).
文摘[Objectives]To study the quality standard of Chinese drug in compound Qianglinaogingsu Tablets(Fructus Schisandrae Chinensis and Coru Cervi Pantotrichum).[Methods]The thin layer chromatography(TLC)differentiation method was established for Fructus Schisandrae Chinensis and Cornu Cervi Pantotrichum.[Results]TLC spots developed were fairly clear,and the blank test showed no interference.[Conclusions]The method is fast,simple and can be used for quality control of Qianglinaoqingsu Tablets.
基金Supported by Key R&D Projects of Guangxi Science and Technology Department (GUIKE AB21196057)Basic Scientific Research Ability Improvement Project of Young and Middle-aged Teachers in Guangxi Universities and Colleges in 2019 (2019KY0341)+1 种基金Open Project of Guangxi Zhuang Yao Medicine Key Laboratory (GXZYKF2020A-08)Zhuang Pharmacy,a Key Discipline of Traditional Chinese Medicine (Ethnic Pharmacy) in the"12^th Five-year" Plan of the State Administration of Traditional Chinese Medicine
文摘[Objectives]Taking chlorogenic acid as index component,TLC and content determination method of Laggerae Alatae Herba were established.[Methods]TLC identification used silica gel G thin-layer plate,and butyl acetate∶formic acid∶water(7∶2.5∶2.5)was taken as developing agent,and it was inspected under ultraviolet lamp(365 nm).The content was determined by chromatographic column Inertsil ODS-3 C_(18)(4.60 mm×250 mm,5μm).Mobile phase:methanol-0.1%phosphoric acid(28∶72);detection wavelength:329 nm;flow speed:1.0 mL/min;column temperature:25℃;injection volume:10μL.[Results]Chlorogenic acid can be detected by TLC,with clear spots and good specificity.When injection volume of chlorogenic acid was between 0.099 and 0.990μg(R^(2)=0.9999),there was good linear relationship.In low,medium and high sample adding groups of Laggerae Alatae Herba,average recovery rates of chlorogenic acids were 98.80%(RSD=2.09%),98.24%(RSD=1.96%)and 99.65%(RSD=2.15%).[Conclusions]The method could effectively identify medicinal material Laggerae Alatae Herba,and accurately measure the content of chlorogenic acid in Laggerae Alatae Herba,thereby providing a scientific basis for developing and using medicinal resources of Laggerae Alatae Herba.
文摘This letter proposes an effective and robust speech feature extraction method based on statistical analysis of Pitch Frequency Distributions (PFD) for speaker identification. Compared with the conventional cepstrum, PFD is relatively insensitive to Additive White Gaussian Noise (AWGN), but it does not show good performance for speaker identification, even if under clean environments. To compensate this shortcoming, PFD and conventional cepstrum are combined to make the ultimate decision, instead of simply taking one kind of features into account.Experimental results indicate that the hybrid approach can give outstanding improvement for text-independent speaker identification under noisy environments corrupted by AWGN.
文摘In this paper, an efficient model of palmprint identification is presented based on subspace density estimation using Gaussian Mixture Model (GMM). While a few training samples are available for each person, we use intrapersonal palmprint deformations to train the global GMM instead of modeling GMMs for every class. To reduce the dimension of such variations while preserving density function of sample space, Principle Component Analysis (PCA) is used to find the principle differences and form the Intrapersonal Deformation Subspace (IDS). After training GMM using Expectation Maximization (EM) algorithm in IDS, a maximum likelihood strategy is carried out to identify a person. Experimental results demonstrate the advantage of our method compared with traditional PCA method and single Gaussian strategy.
文摘Anatomical and TLC study of three Chinese peonies were conducted to make a comparative analysis. Peonies (Paeonia suffruticosa (tree peony), Paeonia lactiflora (Chinese peony) and Paeonia veitchii (Chinese peony)) are traditionally used on the Qinghai-Tibet Plateau in China. Recent studies have shown that the peonies have different pharmacological activities and clinical applications. To distinguish these three species of peonies and ensure the safety and effectiveness in their use, the microscopic characteristics and chromatographic profile of their roots and the corresponding powder were studied. Plant materials sectioned and stained and the raw powder were studied with an optical microscope using standard techniques in microscopy. The results of microscopic features and TLC were described and illustrated. The three species have different microscopic characteristics and TLC profiles, which allow us to distinguish them. In fact, with the help of features semi-quantitative and qualitative, an identification key was developed in our work and illustrated with photos and a table. The aim of our work was to show that the optical microscopy and related techniques provide a achievable practicality, which can be applied without ambiguity to the authentication of species peonies.
基金Scientific Research Project for School-level Teachers of Sichuan College of Traditional Chinese Medicine in 2023(23ZRYB08)Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory Open Fund Project of Southwest Minzu University(QTPEM2305).
文摘[Objectives]To study the original plants,characters,tissue structure,powder characteristics and thin-layer chromatography(TLC)characteristics of Cardamine tangutorum and Cardamine macrophylla as Tibetan and Qiang edible and medicinal herbs,and to provide the basis for the identification of C.tangutorum and C.macrophylla.[Methods]The identification of C.macrophyll and C.tangutorum was carried out by original plant identification,character identification,microscopic identification and TLC identification.[Results]C.tangutorum and C.macrophylla can be distinguished according to the shape of rhizome and stem,the difference of stem leaves and leaflets,and the difference of flower color;there is no obvious difference between the characteristics of the shape and the powder;the thin layer chromatography shows that in the thin layer chromatography of C.tangutorum and C.macrophylla,spots with the same color are shown on the corresponding positions of the ground part and the reference substance quercetin;the underground part and the position corresponding to the reference substanceβ-sitosterol all show the same color spots.[Conclusions]This study provides a reference for the identification of C.tangutorum and C.macrophylla.
基金National Key Research and Development Program(2018YFC1708005)Science and Technology Program of Sichuan Province(2021YFS0043)Special Fund Research Projects of Fundamental Research Funds for the Central Universities(2020NGD01).
文摘[Objectives]To explore morphological identification,macroscopical identification,microscopic identification,thin layer chromatography(TLC)identification of Tibetan medical material Dracocephalum tanguticum Maxim.,and provide experimental data for its identification and application.[Methods]The Tibetan medical material was identified by means of original plant,characters,powder,paraffin section and thin layer chromatography(TLC).[Results]Tibetan medical material D.tanguticum Maxim.was obviously distinguished in character identification and microscopic identification,and the TLC method was simple and feasible.[Conclusions]The results will provide the source work foundation for the formulation of the quality standard of Sichuan Province(draft)for Tibetan medicinal material"D.tanguticum Maxim."and the development of pharmaceutical preparations for medical institutions.