Molecular biology covers a wide scope of problems related to molecular and cell biology including structural and functional genomics, transcriptomics, proteomics, bio- informatics, and biomedicines. Molecular biology ...Molecular biology covers a wide scope of problems related to molecular and cell biology including structural and functional genomics, transcriptomics, proteomics, bio- informatics, and biomedicines. Molecular biology is the study of molecular underpinnings of the processes of replication, transcription, translation, and cell function. The central dogma of molecular biology where genetic material is transcribed into RNA and then translated into proteins, despite being oversimplified, still provides a good starting point for understanding the field. The picture has been revised in light of emerging novel roles for RNA. Increasingly many other areas of biology focus on molecules, either directly or indirectly studying the interactions in their own right. The techniques and methods of molecular biology, such as molecular cloning, polymerase chain reaction, gel electrophoresis, macromolecule blotting and probing, microarray and so on, have been applied in biomedicine and herbal medicines.展开更多
Radiation-induced pulmonary injury is a major dose-limiting toxicity that occurs due to thoracic radiotherapy.And the most widely used high-throughput technology,16S ribosomal RNA sequencing,is adopted for bacterial c...Radiation-induced pulmonary injury is a major dose-limiting toxicity that occurs due to thoracic radiotherapy.And the most widely used high-throughput technology,16S ribosomal RNA sequencing,is adopted for bacterial classification and identification of airway microbiome analysis.In this study,we provide novel insights into the composition pattern and predicted function of the lung microbiome in RIPI rats.Certain genus and species change in a dose-dependent manner,which might be capable of serving as novel bacterial biomarkers,as well as the potential targets for the future diagnosis or treatment of RIPI.In summary,our findings provide a vital reference for RIPI study,and will contribute to investigate the novel therapeutic or preventive management for RIPI.展开更多
文摘Molecular biology covers a wide scope of problems related to molecular and cell biology including structural and functional genomics, transcriptomics, proteomics, bio- informatics, and biomedicines. Molecular biology is the study of molecular underpinnings of the processes of replication, transcription, translation, and cell function. The central dogma of molecular biology where genetic material is transcribed into RNA and then translated into proteins, despite being oversimplified, still provides a good starting point for understanding the field. The picture has been revised in light of emerging novel roles for RNA. Increasingly many other areas of biology focus on molecules, either directly or indirectly studying the interactions in their own right. The techniques and methods of molecular biology, such as molecular cloning, polymerase chain reaction, gel electrophoresis, macromolecule blotting and probing, microarray and so on, have been applied in biomedicine and herbal medicines.
基金This study was supported by the National Natural Science Foundation of China(No.81773226,U1967220,and 82073476)Key Laboratory of Radiation Medicine and Protection,Soochow University(GZN1202101)+1 种基金China Postdoctoral Science Foundation(No.2017M611908 and 2017M610351)Jiangsu Province Postdoctoral Science Foundation(No.1701177B).
文摘Radiation-induced pulmonary injury is a major dose-limiting toxicity that occurs due to thoracic radiotherapy.And the most widely used high-throughput technology,16S ribosomal RNA sequencing,is adopted for bacterial classification and identification of airway microbiome analysis.In this study,we provide novel insights into the composition pattern and predicted function of the lung microbiome in RIPI rats.Certain genus and species change in a dose-dependent manner,which might be capable of serving as novel bacterial biomarkers,as well as the potential targets for the future diagnosis or treatment of RIPI.In summary,our findings provide a vital reference for RIPI study,and will contribute to investigate the novel therapeutic or preventive management for RIPI.
