Hepatocellular carcinoma （HCC） is one of the most deadly human cancers, but it is very difficult to establish an animal model by using surgical specimens. In the present experiment, histologically intact fresh surgi...Hepatocellular carcinoma （HCC） is one of the most deadly human cancers, but it is very difficult to establish an animal model by using surgical specimens. In the present experiment, histologically intact fresh surgical specimens of HCC were subcutaneously transplanted in non-obese diabetic/severe combined immunodeficienccy （NOD/SCID） mice. The biological characteristics of the original and the corresponding transplanted tumors and cell lines were investigated. The results showed that 5 new animal models and 2 primary cell lines were successfully established f^om surgical specimens. Hematoxylin-eosin staining showed that xenografts retained major histological features of the original surgical specimens. The two new cell lines had been cultivated for 3 years and successively passaged for more than 100 passages in vitro. The morphological characteristics and biologic features of the two cell lines were genetically similar to the original tumor. The subcutaneous transplant animal models with histologically intact tumor tissue and primary cell lines could be useful for in vivo and in vitro testing of anti-cancer drugs and be ideal models to study various biologic features of HCC.展开更多
Quality of cement is evaluated via group of tests. The most important, and close to understanding, is the compressive strength test. Recently, Egyptian standards adopted the European standards EN-196 and EN-197 for sp...Quality of cement is evaluated via group of tests. The most important, and close to understanding, is the compressive strength test. Recently, Egyptian standards adopted the European standards EN-196 and EN-197 for specifying and evaluating quality of cements. This was motivated by the large European investments in the local production of cement. The current study represents a comparative investigation, experimental and numerical, of the effect of different parameters on evaluation of compressive strength. Main parameters are shape of specimens and type of sand used for producing tested mortars. Three sets of specimens were made for ten types of cements. First set were 70.6 mm cubes molded according to old standards using single sized sand. Second group were prisms molded from standard sand (CEN sand) according to the recent standards. Third group were prisms molded from local sand sieved and regenerated to simulate same grading of CEN sand. All specimens were cured according to relevant standards and tested at different ages (2,3,7,10 and 28 days). Results show that CEM-I Type of cement does not fulfill, in all of its grades, the strength requirements of Ordinary Portland cement OPC specified in old standards. Also, the use of simulated CEN sand from local source gives strengths lower than those obtained using standard certified CEN sand. A limited number of tests were made on concrete specimens from two most common CEM-I types to investigate effect on concrete strength and results were also reported. Numerical investigation of the effect of specimen shape and type of sand on evaluation of compressive strength of mortar specimens, presented in the current study, applies one of the artificial intelligence techniques to simulate and predict the strength behavior at different ages. The Artificial Neural Network (ANN) technique is introduced in the current study to simulate the strength behavior using the available experimental data and predict the strength value at any age in the range of the experiments or in the future. The results of the numerical study showed that the ANN method with less effort was very efficiently capable of simulating the effect of specimen shape and type of sand on the strength behavior of tested mortar with different cement types.展开更多
In order to achieve a better understanding of failure behavior of cruciform specimen under different biaxial loading conditions,a three-dimensional finite element model is established with solid and interface elements...In order to achieve a better understanding of failure behavior of cruciform specimen under different biaxial loading conditions,a three-dimensional finite element model is established with solid and interface elements.Maximum stress criterion,two Hashin-type criteria and the new proposed criteria are used to predict the strength of plain woven textile composites when biaxial loading ratio equals 1.Compared with experimental data,only the new proposed criteria can reach reasonable results.The applicability of the new proposed criteria is also verified by predicting the tensile and compressive strength of cruciform specimen under different biaxial loading ratios.Moreover,the introduction of interface element makes it more intuitive to recognize delamination failure.The shape of the predicted delamination failure region in the interface layer is similar to that of the failure region in neighboring entity layers,but the area of delamination failure region is a little larger.展开更多
In this paper,the failure features of strip tensile specimen were observed and analysed for need-le-punched non-woven geotextiles.A mechanical model which expresses the relation between thetensile modulus,the strip sp...In this paper,the failure features of strip tensile specimen were observed and analysed for need-le-punched non-woven geotextiles.A mechanical model which expresses the relation between thetensile modulus,the strip specimen size,contraction factor and the tensile strength of non-wovenfabric was derived.The theoretical prediction showed that the main factor influencing tensilestrength of non-woven geotextile specimens with different size is the contraction factor of specimenor the specimen aspect ratio(width/length).The larger the aspect ratio,the higher the tensilestrength test value of geotextiles,but the experiments showed that the specimen tensile strength isnot increased with increasing the width of specimen.The reason was discussed and it seemed thatthe deviation could be served as an indication of the degree of imperfectness of the non-wovenstructure.展开更多
基金supported by grants from the National Key Sci-Tech Special Project of China (2008ZX10002-022)Leading Academic Discipline Project of Shanghai Municipal Education Committee (J50208)
文摘Hepatocellular carcinoma （HCC） is one of the most deadly human cancers, but it is very difficult to establish an animal model by using surgical specimens. In the present experiment, histologically intact fresh surgical specimens of HCC were subcutaneously transplanted in non-obese diabetic/severe combined immunodeficienccy （NOD/SCID） mice. The biological characteristics of the original and the corresponding transplanted tumors and cell lines were investigated. The results showed that 5 new animal models and 2 primary cell lines were successfully established f^om surgical specimens. Hematoxylin-eosin staining showed that xenografts retained major histological features of the original surgical specimens. The two new cell lines had been cultivated for 3 years and successively passaged for more than 100 passages in vitro. The morphological characteristics and biologic features of the two cell lines were genetically similar to the original tumor. The subcutaneous transplant animal models with histologically intact tumor tissue and primary cell lines could be useful for in vivo and in vitro testing of anti-cancer drugs and be ideal models to study various biologic features of HCC.
文摘Quality of cement is evaluated via group of tests. The most important, and close to understanding, is the compressive strength test. Recently, Egyptian standards adopted the European standards EN-196 and EN-197 for specifying and evaluating quality of cements. This was motivated by the large European investments in the local production of cement. The current study represents a comparative investigation, experimental and numerical, of the effect of different parameters on evaluation of compressive strength. Main parameters are shape of specimens and type of sand used for producing tested mortars. Three sets of specimens were made for ten types of cements. First set were 70.6 mm cubes molded according to old standards using single sized sand. Second group were prisms molded from standard sand (CEN sand) according to the recent standards. Third group were prisms molded from local sand sieved and regenerated to simulate same grading of CEN sand. All specimens were cured according to relevant standards and tested at different ages (2,3,7,10 and 28 days). Results show that CEM-I Type of cement does not fulfill, in all of its grades, the strength requirements of Ordinary Portland cement OPC specified in old standards. Also, the use of simulated CEN sand from local source gives strengths lower than those obtained using standard certified CEN sand. A limited number of tests were made on concrete specimens from two most common CEM-I types to investigate effect on concrete strength and results were also reported. Numerical investigation of the effect of specimen shape and type of sand on evaluation of compressive strength of mortar specimens, presented in the current study, applies one of the artificial intelligence techniques to simulate and predict the strength behavior at different ages. The Artificial Neural Network (ANN) technique is introduced in the current study to simulate the strength behavior using the available experimental data and predict the strength value at any age in the range of the experiments or in the future. The results of the numerical study showed that the ANN method with less effort was very efficiently capable of simulating the effect of specimen shape and type of sand on the strength behavior of tested mortar with different cement types.
基金supported by the National Natural Science Foundation of China(No.51205190)the Jiangsu Province Key Laboratory of Aerospace Power System(No.NJ20140019)
文摘In order to achieve a better understanding of failure behavior of cruciform specimen under different biaxial loading conditions,a three-dimensional finite element model is established with solid and interface elements.Maximum stress criterion,two Hashin-type criteria and the new proposed criteria are used to predict the strength of plain woven textile composites when biaxial loading ratio equals 1.Compared with experimental data,only the new proposed criteria can reach reasonable results.The applicability of the new proposed criteria is also verified by predicting the tensile and compressive strength of cruciform specimen under different biaxial loading ratios.Moreover,the introduction of interface element makes it more intuitive to recognize delamination failure.The shape of the predicted delamination failure region in the interface layer is similar to that of the failure region in neighboring entity layers,but the area of delamination failure region is a little larger.
文摘In this paper,the failure features of strip tensile specimen were observed and analysed for need-le-punched non-woven geotextiles.A mechanical model which expresses the relation between thetensile modulus,the strip specimen size,contraction factor and the tensile strength of non-wovenfabric was derived.The theoretical prediction showed that the main factor influencing tensilestrength of non-woven geotextile specimens with different size is the contraction factor of specimenor the specimen aspect ratio(width/length).The larger the aspect ratio,the higher the tensilestrength test value of geotextiles,but the experiments showed that the specimen tensile strength isnot increased with increasing the width of specimen.The reason was discussed and it seemed thatthe deviation could be served as an indication of the degree of imperfectness of the non-wovenstructure.
