AIM:To develop orthotopic gastric cancer mouse models from different cell lines and characterize the tumor features to assist further in preclinical trials and clinical treatment strategies.METHODS:Human gastric cance...AIM:To develop orthotopic gastric cancer mouse models from different cell lines and characterize the tumor features to assist further in preclinical trials and clinical treatment strategies.METHODS:Human gastric cancer SGC-7901 and BGC823 cell suspensions were injected subcutaneously into nude mice to develop solid tumors,and tumor tissue pieces were then implanted under the serous coat of the stomach.An autopsy was performed on all animals of the SGC-7901 and BGC-823 models to observe the primary tumor growth and metastases using pathological and immunohistochemical methods.RESULTS:Both models showed large tumors in situ resulting in pressure and infiltration of the adjacent organs.The gastric cavity became smaller,along with stenosis of the cardia or pylorus.There were biological and statistical differences between the two models.The metastasis rate in involved organs (lymph nodes,kidney,spleen,testis) was significantly higher in the BGC-823 model compared to the SGC-7901 model (P < 0.05 or P < 0.01).The median survival of the BGC-823 model was shorter than that of SGC-7901 (23 d vs 84 d,P < 0.05).Histopathologically,the primary tumor and metastatic lesions of the two models showed obvious atypia and mucus in the cytoplasm.Compared with the SGC-7901 model,BGC-823 appeared more poorly differentiated (absence of adenoid structure),had a smaller volume,and richer capillary structure.Immunohistochemical staining revealed cytokeratin 20 and epithelial membrane antigen expression was positive in the SGC-7901 tumors,while negative in BGC-823 ones.CONCLUSION:Models using the SGC-7901 and BGC-823 cell lines were established which could function in gastric cancer research on carcinogenesis mechanism and drug discovery.The two models showed different tumor behavior and the latter was more malignant than the former.展开更多
AIM:To establish a gastric cancer nude-mouse model with improved orthotopic implantation and investigate its biological characteristics at different time points.METHODS:Human gastric cancer SGC-7901 cell suspensions w...AIM:To establish a gastric cancer nude-mouse model with improved orthotopic implantation and investigate its biological characteristics at different time points.METHODS:Human gastric cancer SGC-7901 cell suspensions were injected subcutaneously into a nude mouse to develop solid tumors,and the tumor tissue pieces were implanted under the serous coat.The nude mice were then euthanized in group every two weeks to observe the primary tumor growth and metastases.RESULTS:Within 2-4 wk,there were no obvious chang-es about the primary tumor in stomach.At the sixth week,the primary tumor began to grow fast,resulting in incrassation of the gastric wall and stenosis of the gastric cavity,and metastases into the liver and lymph nodes were detected.The tumor,which compressed the adjacent organs,gradually became bigger and bigger followed by stenosis or vanishment of the gastric cavity from 8 to 12 wk.There were massive metastases,and the rate of metastasis was 58%in lymph nodes,78%in liver,39%in kidney,and 81%in peritoneum or septum.CONCLUSION:A gastric cancer model is established,which can simulate the clinical tumor behavior and provide experimental carrier for clinical trials of gastric cancer treatment.展开更多
【目的】以3种不同方法构建BALB/c裸鼠BEL-7402人肝癌模型,并观察BALB/c裸鼠肿瘤生长情况。【方法】将51只BALB/c裸鼠分为A、B、C 3组,每组17只。A组动物皮下注射0.2 m L BEL-7402人肝癌细胞,B组动物于肝脏原位注射0.05 mL BEL-7402人...【目的】以3种不同方法构建BALB/c裸鼠BEL-7402人肝癌模型,并观察BALB/c裸鼠肿瘤生长情况。【方法】将51只BALB/c裸鼠分为A、B、C 3组,每组17只。A组动物皮下注射0.2 m L BEL-7402人肝癌细胞,B组动物于肝脏原位注射0.05 mL BEL-7402人肝癌细胞与Matrigel Matrix的混合物,C组动物于肝脏原位接种BEL-7402人肝癌细胞瘤块组织。从接种后第2周至第5周,每周测定裸鼠体质量,且每组随机取3只动物解剖取肿瘤测量其大小和质量。第5周后,观察记录各组剩余动物(每组5只)的一般状态和存活天数。采用苏木素—伊红(HE)染色观察肿瘤组织病理学变化。【结果】A、B、C 3组实验动物成瘤率均为100%。5周后,A、B、C 3组剩余动物存活天数分别为(90.8±10.2)、(75.6±14.0)、(67.4±11.1) d。解剖所取肿瘤呈结节状,组织病理学检查可见肿瘤细胞呈一定方向排列,细胞呈梭形、多边形,异型明显,可见核分裂。【结论】本实验所采用的3种不同方法均能成功建立BALB/c裸鼠BEL-7402人肝癌模型。展开更多
目的探索高频彩色多普勒超声与实际游标卡尺测量肿瘤的相关性。方法将0.10 mL 2×106DU145细胞悬液注射到裸鼠前列腺包膜下。细胞注射后第2周至第5周使用高频彩色多普勒超声测量裸鼠肿瘤横径与纵径。麻醉状态下解剖裸鼠,游离肿瘤,...目的探索高频彩色多普勒超声与实际游标卡尺测量肿瘤的相关性。方法将0.10 mL 2×106DU145细胞悬液注射到裸鼠前列腺包膜下。细胞注射后第2周至第5周使用高频彩色多普勒超声测量裸鼠肿瘤横径与纵径。麻醉状态下解剖裸鼠,游离肿瘤,游标卡尺测量肿瘤横径与纵径。肿瘤放入1.3 mol.L-1甲醛中固定,HE染色。结果高频彩色多普勒超声测量的横径与使用游标卡尺测量的横径相比,Pearson与Spearm an相关系数分别是0.996(P<0.001)与0.964(P<0.001);高频彩色多普勒超声测量的横径与使用游标卡尺测量的纵径相比,Pearson与Spearm an相关系数分别是0.988(P<0.001)与0.898(P<0.01)。结论高频彩色多普勒超声可精确地监测裸鼠前列腺原位种植肿瘤的生长。展开更多
基金Supported by A grant from the Natural Science Foundation of China,No.30830040
文摘AIM:To develop orthotopic gastric cancer mouse models from different cell lines and characterize the tumor features to assist further in preclinical trials and clinical treatment strategies.METHODS:Human gastric cancer SGC-7901 and BGC823 cell suspensions were injected subcutaneously into nude mice to develop solid tumors,and tumor tissue pieces were then implanted under the serous coat of the stomach.An autopsy was performed on all animals of the SGC-7901 and BGC-823 models to observe the primary tumor growth and metastases using pathological and immunohistochemical methods.RESULTS:Both models showed large tumors in situ resulting in pressure and infiltration of the adjacent organs.The gastric cavity became smaller,along with stenosis of the cardia or pylorus.There were biological and statistical differences between the two models.The metastasis rate in involved organs (lymph nodes,kidney,spleen,testis) was significantly higher in the BGC-823 model compared to the SGC-7901 model (P < 0.05 or P < 0.01).The median survival of the BGC-823 model was shorter than that of SGC-7901 (23 d vs 84 d,P < 0.05).Histopathologically,the primary tumor and metastatic lesions of the two models showed obvious atypia and mucus in the cytoplasm.Compared with the SGC-7901 model,BGC-823 appeared more poorly differentiated (absence of adenoid structure),had a smaller volume,and richer capillary structure.Immunohistochemical staining revealed cytokeratin 20 and epithelial membrane antigen expression was positive in the SGC-7901 tumors,while negative in BGC-823 ones.CONCLUSION:Models using the SGC-7901 and BGC-823 cell lines were established which could function in gastric cancer research on carcinogenesis mechanism and drug discovery.The two models showed different tumor behavior and the latter was more malignant than the former.
基金Supported by the Natural Science Foundation of China,No. 30830040
文摘AIM:To establish a gastric cancer nude-mouse model with improved orthotopic implantation and investigate its biological characteristics at different time points.METHODS:Human gastric cancer SGC-7901 cell suspensions were injected subcutaneously into a nude mouse to develop solid tumors,and the tumor tissue pieces were implanted under the serous coat.The nude mice were then euthanized in group every two weeks to observe the primary tumor growth and metastases.RESULTS:Within 2-4 wk,there were no obvious chang-es about the primary tumor in stomach.At the sixth week,the primary tumor began to grow fast,resulting in incrassation of the gastric wall and stenosis of the gastric cavity,and metastases into the liver and lymph nodes were detected.The tumor,which compressed the adjacent organs,gradually became bigger and bigger followed by stenosis or vanishment of the gastric cavity from 8 to 12 wk.There were massive metastases,and the rate of metastasis was 58%in lymph nodes,78%in liver,39%in kidney,and 81%in peritoneum or septum.CONCLUSION:A gastric cancer model is established,which can simulate the clinical tumor behavior and provide experimental carrier for clinical trials of gastric cancer treatment.
文摘【目的】以3种不同方法构建BALB/c裸鼠BEL-7402人肝癌模型,并观察BALB/c裸鼠肿瘤生长情况。【方法】将51只BALB/c裸鼠分为A、B、C 3组,每组17只。A组动物皮下注射0.2 m L BEL-7402人肝癌细胞,B组动物于肝脏原位注射0.05 mL BEL-7402人肝癌细胞与Matrigel Matrix的混合物,C组动物于肝脏原位接种BEL-7402人肝癌细胞瘤块组织。从接种后第2周至第5周,每周测定裸鼠体质量,且每组随机取3只动物解剖取肿瘤测量其大小和质量。第5周后,观察记录各组剩余动物(每组5只)的一般状态和存活天数。采用苏木素—伊红(HE)染色观察肿瘤组织病理学变化。【结果】A、B、C 3组实验动物成瘤率均为100%。5周后,A、B、C 3组剩余动物存活天数分别为(90.8±10.2)、(75.6±14.0)、(67.4±11.1) d。解剖所取肿瘤呈结节状,组织病理学检查可见肿瘤细胞呈一定方向排列,细胞呈梭形、多边形,异型明显,可见核分裂。【结论】本实验所采用的3种不同方法均能成功建立BALB/c裸鼠BEL-7402人肝癌模型。
