The changes in 5α-reductase (type 2 ) gene expression in the epi-didymis of puberty diabetic rats were studied by the Northern blot and Dotblot method. Rats were divided into 3 groups: the control group (C), thediabe...The changes in 5α-reductase (type 2 ) gene expression in the epi-didymis of puberty diabetic rats were studied by the Northern blot and Dotblot method. Rats were divided into 3 groups: the control group (C), thediabetic group (D), and the diabetic group with insulin treatment (DI).Results: The Northern blot intensity of the caput epididymis in Group D is展开更多
BACKGROUND Quantitative studies on the changes in inflammation-related content in tears,especially the effect of diabetes,are lacking.In this study,we measured the preoperative and postoperative tear inflammatory medi...BACKGROUND Quantitative studies on the changes in inflammation-related content in tears,especially the effect of diabetes,are lacking.In this study,we measured the preoperative and postoperative tear inflammatory mediator levels in cataract patients,focusing on the expression of inflammatory factors in postoperative cataracts in the diabetic,and investigated the effect of drugs on the control of postoperative inflammation.AIM To study the expression of inflammatory factors in elderly people with type 2 diabetes after cataract surgery.METHODS Patients with a mean age of 70.3±6.3 years were divided into group A(composed of elderly patients with cataracts and type 2 diabetes,n=20 eyes)and group B(patients with age-related cataract,n=20 eyes).Their tears were collected before each operation and on days 1 and 3,and weeks 1,2,3,and 4 post-surgery.Saline(150μL)was dropped into the conjunctival sac of the surgical eye,followed by oculogyration in four directions.The fluid in the conjunctival sac was extracted using a sterile syringe and stored in Eppendorf tubes at-80°C until measurement.The expression levels of matrix metalloproteinase-2(MMP-2),MMP-9,tissue inhibitor of metalloproteinase-1(TIMP-1),TIMP-2,interleukin-6(IL-6),and IL-20 in tear fluid were measured using enzyme-linked immunosorbent assays.RESULTS The postoperative expression levels of MMP-2,MMP-9,TIMP-2,IL-6,and IL-20 in group A were significantly higher than those in group B,whereas the concentration of TIMP-1 in group A remained lower than that in group B.The levels of MMP-2 and IL-6 in both groups continuously increased until the peak in the first postoperative week,and then gradually decreased over the next three weeks.Ultimately,MMP-2 declined to a lower level than that preoperatively at week 4,but IL-6 decreased to the same level as that preoperatively.The level of MMP-9 peaked in the first two weeks postoperative and then returned to the same level as 1-day post-operation.The concentration of TIMP-1 post-operation remained constant at a lower level than before surgery,and TIMP-2 Levels remained stable in both groups.IL-20 content started to increase in the third week after surgery.CONCLUSION Inflammatory factor levels in tears fluctuated before and post-operation,which indicated more severe postoperative inflammation in the first two weeks.展开更多
目的:评价基于跨理论模型的护理干预对2型糖尿病病人自我管理能力等方面的干预效果。方法:检索PubMed、Web of Science、the Cochrane Library、Embase、中国知网、维普数据库、万方数据库和中国生物医学文献数据库,检索时限为建库至202...目的:评价基于跨理论模型的护理干预对2型糖尿病病人自我管理能力等方面的干预效果。方法:检索PubMed、Web of Science、the Cochrane Library、Embase、中国知网、维普数据库、万方数据库和中国生物医学文献数据库,检索时限为建库至2023年1月11日,收集基于跨理论模型的护理干预提高2型糖尿病病人自我管理能力的随机对照试验,采用RevMan 5.2软件进行数据分析。结果:共纳入12篇文献,共计1513例病人。Meta分析结果显示,基于跨理论模型的护理干预可提高病人自我管理能力[SMD=0.69,95%CI(0.50,0.88),P<0.05]、自我效能[SMD=0.85,95%CI(0.64,1.06),P<0.05],改善生活质量[SMD=0.79,95%CI(0.18,1.40),P<0.05]。结论:现有证据表明基于跨理论模型的护理干预可以有效提高2型糖尿病病人的自我管理能力和自我效能,改善病人的生活质量,目前还需要大样本、高质量的临床研究验证结果。展开更多
Context: Physical exercise is a key component of treatment and management of people with Type 2 Diabetes, but the role of strategies and interventions to increase and promote physical activity is unclear. Objective: T...Context: Physical exercise is a key component of treatment and management of people with Type 2 Diabetes, but the role of strategies and interventions to increase and promote physical activity is unclear. Objective: To conduct a systematic review of intervention studies assessing the effects of physical activity counselling on health outcomes in subjects with type 2 diabetes mellitus. Data sources: The Cochrane Library, MEDLINE, EMBASE, CINAHL, LILACS, ClinicalTrials.gov databases were used for the literature search. Data Selection: Studies that assessed the effects of interventions mainly based on physical activity counselling strategies, on physical activity level and glycosylated hemoglobin (HbA1c) were included in the review. Data Extraction: Two independent reviewers extracted the data. Data Synthesis: A total of 23 studies out of 1425 retrieved from databases search, were included in the review. The global number of subjects included in the selected studies was 9913, and the mean age of participants was 58.8 (±8.2) years (min = 46.3;max = 73.6). The most part of the studies (19) reported values of physical activity level;13 of them (68.4%) found a significant effect after the counselling intervention, while 6 (31.6%) did not found significant changes. Among the 17 studies reporting data on HbA1c 9 (52.9%) described a significant decrease in the counselling intervention group, while 7 (41.2%) did not find any statistically significant change and 1 (5.9%) reported a significant HbA1c reduction in the comparison group rather than the counselling group. Conclusions: The results presented in this systematic review seem to affirm the usefulness of physical counselling interventions on physical activity and HbA1c, however the lack of homogeneity in the intervention protocols and the contrasting results limit the comprehension of the usefulness of such an approach in patient with type 2 diabetes.展开更多
Objective: This study looks to review different methods of lifestyle changing interventions, examine and compare them to determine usefulness, efficiency, and outcome in regard to future ventures to help control the r...Objective: This study looks to review different methods of lifestyle changing interventions, examine and compare them to determine usefulness, efficiency, and outcome in regard to future ventures to help control the rise of this type 2 diabetes. Methods: Three systematic reviews were found and compared based on efficacy and key differences in their approach to the ever-growing problem of type 2 diabetes. Outcomes and intervention styles of the studies were noted and critiqued based on their statistical significance. Conclusion: The first study revealed that multiple behavior change techniques (BCTs) correlate with a reduction in weight and HbA1c, especially when they work synergistically alongside one another. The second study spoke of how community intervention had an inclusive quality about it, which allowed for population variability to not affect implementation. It also showed that small incremental changes were best when trying to establish a change long-term across a large population. Finally, the third study revealed that, as the participant got closer to maintaining a good physical activity regiment, their self-efficacy also increased. The attributes of these studies give evidence that interventions that use multiple approaches are most effective when looking to improve physical activity and diet in Type 2 diabetics.展开更多
文摘The changes in 5α-reductase (type 2 ) gene expression in the epi-didymis of puberty diabetic rats were studied by the Northern blot and Dotblot method. Rats were divided into 3 groups: the control group (C), thediabetic group (D), and the diabetic group with insulin treatment (DI).Results: The Northern blot intensity of the caput epididymis in Group D is
基金Supported by Cataract Prevention and Control Appropriate Technology Base of Sichuan Provincial Health Commission(Regional Demonstration),No.2022JDXM012。
文摘BACKGROUND Quantitative studies on the changes in inflammation-related content in tears,especially the effect of diabetes,are lacking.In this study,we measured the preoperative and postoperative tear inflammatory mediator levels in cataract patients,focusing on the expression of inflammatory factors in postoperative cataracts in the diabetic,and investigated the effect of drugs on the control of postoperative inflammation.AIM To study the expression of inflammatory factors in elderly people with type 2 diabetes after cataract surgery.METHODS Patients with a mean age of 70.3±6.3 years were divided into group A(composed of elderly patients with cataracts and type 2 diabetes,n=20 eyes)and group B(patients with age-related cataract,n=20 eyes).Their tears were collected before each operation and on days 1 and 3,and weeks 1,2,3,and 4 post-surgery.Saline(150μL)was dropped into the conjunctival sac of the surgical eye,followed by oculogyration in four directions.The fluid in the conjunctival sac was extracted using a sterile syringe and stored in Eppendorf tubes at-80°C until measurement.The expression levels of matrix metalloproteinase-2(MMP-2),MMP-9,tissue inhibitor of metalloproteinase-1(TIMP-1),TIMP-2,interleukin-6(IL-6),and IL-20 in tear fluid were measured using enzyme-linked immunosorbent assays.RESULTS The postoperative expression levels of MMP-2,MMP-9,TIMP-2,IL-6,and IL-20 in group A were significantly higher than those in group B,whereas the concentration of TIMP-1 in group A remained lower than that in group B.The levels of MMP-2 and IL-6 in both groups continuously increased until the peak in the first postoperative week,and then gradually decreased over the next three weeks.Ultimately,MMP-2 declined to a lower level than that preoperatively at week 4,but IL-6 decreased to the same level as that preoperatively.The level of MMP-9 peaked in the first two weeks postoperative and then returned to the same level as 1-day post-operation.The concentration of TIMP-1 post-operation remained constant at a lower level than before surgery,and TIMP-2 Levels remained stable in both groups.IL-20 content started to increase in the third week after surgery.CONCLUSION Inflammatory factor levels in tears fluctuated before and post-operation,which indicated more severe postoperative inflammation in the first two weeks.
文摘Context: Physical exercise is a key component of treatment and management of people with Type 2 Diabetes, but the role of strategies and interventions to increase and promote physical activity is unclear. Objective: To conduct a systematic review of intervention studies assessing the effects of physical activity counselling on health outcomes in subjects with type 2 diabetes mellitus. Data sources: The Cochrane Library, MEDLINE, EMBASE, CINAHL, LILACS, ClinicalTrials.gov databases were used for the literature search. Data Selection: Studies that assessed the effects of interventions mainly based on physical activity counselling strategies, on physical activity level and glycosylated hemoglobin (HbA1c) were included in the review. Data Extraction: Two independent reviewers extracted the data. Data Synthesis: A total of 23 studies out of 1425 retrieved from databases search, were included in the review. The global number of subjects included in the selected studies was 9913, and the mean age of participants was 58.8 (±8.2) years (min = 46.3;max = 73.6). The most part of the studies (19) reported values of physical activity level;13 of them (68.4%) found a significant effect after the counselling intervention, while 6 (31.6%) did not found significant changes. Among the 17 studies reporting data on HbA1c 9 (52.9%) described a significant decrease in the counselling intervention group, while 7 (41.2%) did not find any statistically significant change and 1 (5.9%) reported a significant HbA1c reduction in the comparison group rather than the counselling group. Conclusions: The results presented in this systematic review seem to affirm the usefulness of physical counselling interventions on physical activity and HbA1c, however the lack of homogeneity in the intervention protocols and the contrasting results limit the comprehension of the usefulness of such an approach in patient with type 2 diabetes.
文摘Objective: This study looks to review different methods of lifestyle changing interventions, examine and compare them to determine usefulness, efficiency, and outcome in regard to future ventures to help control the rise of this type 2 diabetes. Methods: Three systematic reviews were found and compared based on efficacy and key differences in their approach to the ever-growing problem of type 2 diabetes. Outcomes and intervention styles of the studies were noted and critiqued based on their statistical significance. Conclusion: The first study revealed that multiple behavior change techniques (BCTs) correlate with a reduction in weight and HbA1c, especially when they work synergistically alongside one another. The second study spoke of how community intervention had an inclusive quality about it, which allowed for population variability to not affect implementation. It also showed that small incremental changes were best when trying to establish a change long-term across a large population. Finally, the third study revealed that, as the participant got closer to maintaining a good physical activity regiment, their self-efficacy also increased. The attributes of these studies give evidence that interventions that use multiple approaches are most effective when looking to improve physical activity and diet in Type 2 diabetics.
