Mycobacterium tuberculosis, a Gram-positive bacterium of great clinical relevance, is a lethal pathogen owing to its complex physiological characteristics and development of drug resistance. Several molecular genetic ...Mycobacterium tuberculosis, a Gram-positive bacterium of great clinical relevance, is a lethal pathogen owing to its complex physiological characteristics and development of drug resistance. Several molecular genetic tools have been developed in the past few decades to study this microorganism. These tools have been instrumental in understanding how M. tuberculosis became a successful pathogen. Advanced molecular genetic tools have played a significant role in exploring the complex pathways involved in M. tuberculosis pathogenesis. Here, we review various molecular genetic tools used in the study of M. tuberculosis. Further, we discuss the applications of clustered regularly interspaced short palindromic repeat interference（CRISPRi）, a novel technology recently applied in M. tuberculosis research to study target gene functions. Finally, prospective outcomes of the applications of molecular techniques in the field of M. tuberculosis genetic research are also discussed.展开更多
基金supported by the National Mega-project of China for Innovative Drugs(2018ZX09721001-003-003)for Main Infectious Diseases(2017ZX10302301-003-002)+7 种基金the National Natural Science Foundation of China(No.81572037)the grants of Chinese Academy of Sciences(154144KYSB20150045,YJKYYQ20170036,KFZD-SW-207)the Public Research and Capacity Building Project of Guangdong Province(2017A020212004)partially supported by Guangzhou Municipal Industry and Research Collaborative Innovation Program(201508020248,201604020019)the Key Project(SKLRD2016ZJ003)from the State Key Lab of Respiratory Disease,Guangzhou Institute of Respiratory Diseases,First Affiliated Hospital of Guangzhou Medical UniversityT.Z.received support“Science and Technology Innovation Leader of Guangdong Province(2016TX03R095)”CAS-TWAS President's Ph D Fellowship Program(to C.C.and M.M.I.)UCAS Ph D Fellowship Program(to H.M.A.H.and J.M.)for International Students
文摘Mycobacterium tuberculosis, a Gram-positive bacterium of great clinical relevance, is a lethal pathogen owing to its complex physiological characteristics and development of drug resistance. Several molecular genetic tools have been developed in the past few decades to study this microorganism. These tools have been instrumental in understanding how M. tuberculosis became a successful pathogen. Advanced molecular genetic tools have played a significant role in exploring the complex pathways involved in M. tuberculosis pathogenesis. Here, we review various molecular genetic tools used in the study of M. tuberculosis. Further, we discuss the applications of clustered regularly interspaced short palindromic repeat interference（CRISPRi）, a novel technology recently applied in M. tuberculosis research to study target gene functions. Finally, prospective outcomes of the applications of molecular techniques in the field of M. tuberculosis genetic research are also discussed.
