Molecularly imprinted microspheres (MIP) for monocrotophos have been prepared by precipitation polymerization in acetonitrile (ACN) 60℃,24 h,using methacrylic acid (MAA),ethylene glycol dimethacrylate (EGDMA) and 2, ...Molecularly imprinted microspheres (MIP) for monocrotophos have been prepared by precipitation polymerization in acetonitrile (ACN) 60℃,24 h,using methacrylic acid (MAA),ethylene glycol dimethacrylate (EGDMA) and 2, 2-azobisiobutyronitrile (AIBN) as functional monomer,cross-linker and initiator,respectively.The recognition mechanism was elucidated by UV-vis spectra and computer modeling.Equilibrium binding experiment was employed to investigate the rebinding properties,Scatchard analysis showed that specific binding sites formed in the imprinted microspheres,and there were two kinds of binding sites,one was high binding sites,the other was low binding sites.This microspheres can be useful affinity absorbent used for organophosphorus pesticides separation and purification in food and environmental analysis.展开更多
Uniformly imprinted polymer microspheres were prepared by precipitation polymerization in chloroform.MCP（monocrotophos）,MAA（methacrylic acid）,EGDMA（ethylene glycol dimethacrylate） and AIBN（azobisisobutyronitril...Uniformly imprinted polymer microspheres were prepared by precipitation polymerization in chloroform.MCP（monocrotophos）,MAA（methacrylic acid）,EGDMA（ethylene glycol dimethacrylate） and AIBN（azobisisobutyronitrile） were used as template,functional monomer,crosslinker and initiator,respectively.UV and IR spectra were used to demonstrate the mechanism of the interaction between MCP and MAA.It was found that one MCP was entrapped by three MAA molecules in chloroform.Langmuir model was used to fit the adsorption data,the results showed that the MCP imprinted polymer microspheres showed specific binding sites to the MCP.The data obtained in equilibrium adsorption experiments were processed by Scatchard analysis,the dissociation contents(KD1) and the apparent maximum binding capacity (Bmax1) were KD1=0.062?mmol/L,Bmax1=3.26?μmol/g for high affinity binding sites and KD2=3.03?mmol/L,Bmax2=65.70?μmol/g for low affinity binding sites,respectively.The MCP imprinted polymers have showed higher affinity to MCP than to the other organophosphorus pesticide used.So the imprinted polymers microspheres can be used for trace pesticide residue separation,enrichment and analysis.展开更多
以吲哚乙酸（IAA）为模板分子、乙腈为致孔剂,采用沉淀聚合法制备了其分子印迹聚合物,通过静态吸附实验研究了致孔剂乙腈的用量及4-乙烯基吡啶（4-VP）和丙烯酰胺（AA）两种不同性质的功能单体（M）对印迹聚合物吸附性能的影响。结果表...以吲哚乙酸（IAA）为模板分子、乙腈为致孔剂,采用沉淀聚合法制备了其分子印迹聚合物,通过静态吸附实验研究了致孔剂乙腈的用量及4-乙烯基吡啶（4-VP）和丙烯酰胺（AA）两种不同性质的功能单体（M）对印迹聚合物吸附性能的影响。结果表明,以4-VP为M,乙腈的加入量分别为20、25、37.5和50 m L时,制备的印迹聚合物P1~P4均具有良好的分散性,且聚合物P4对IAA具有最显著的印迹效应（印迹因子IF=2.92）;而在同样条件下以AA为M的印迹聚合物的印迹效应较小（IF=1.73）。选择性实验表明,聚合物P4能够从吲哚羧酸同系物中选择性结合IAA。Scatchard分析表明,聚合物P4对IAA表现出两类结合位点,其高亲和力与低亲和力结合位点的平衡离解常数分别为Kd1=0.798 mmol/L和Kd2=7.76 mmol/L,对应的最大结合量分别为21.0和90.7μmol/g。展开更多
基金support by the National Natural Science Foundation of China(No.30371218)High Technology Research and Development Program of China(863 Project No.2004AA649110)
文摘Molecularly imprinted microspheres (MIP) for monocrotophos have been prepared by precipitation polymerization in acetonitrile (ACN) 60℃,24 h,using methacrylic acid (MAA),ethylene glycol dimethacrylate (EGDMA) and 2, 2-azobisiobutyronitrile (AIBN) as functional monomer,cross-linker and initiator,respectively.The recognition mechanism was elucidated by UV-vis spectra and computer modeling.Equilibrium binding experiment was employed to investigate the rebinding properties,Scatchard analysis showed that specific binding sites formed in the imprinted microspheres,and there were two kinds of binding sites,one was high binding sites,the other was low binding sites.This microspheres can be useful affinity absorbent used for organophosphorus pesticides separation and purification in food and environmental analysis.
文摘Uniformly imprinted polymer microspheres were prepared by precipitation polymerization in chloroform.MCP（monocrotophos）,MAA（methacrylic acid）,EGDMA（ethylene glycol dimethacrylate） and AIBN（azobisisobutyronitrile） were used as template,functional monomer,crosslinker and initiator,respectively.UV and IR spectra were used to demonstrate the mechanism of the interaction between MCP and MAA.It was found that one MCP was entrapped by three MAA molecules in chloroform.Langmuir model was used to fit the adsorption data,the results showed that the MCP imprinted polymer microspheres showed specific binding sites to the MCP.The data obtained in equilibrium adsorption experiments were processed by Scatchard analysis,the dissociation contents(KD1) and the apparent maximum binding capacity (Bmax1) were KD1=0.062?mmol/L,Bmax1=3.26?μmol/g for high affinity binding sites and KD2=3.03?mmol/L,Bmax2=65.70?μmol/g for low affinity binding sites,respectively.The MCP imprinted polymers have showed higher affinity to MCP than to the other organophosphorus pesticide used.So the imprinted polymers microspheres can be used for trace pesticide residue separation,enrichment and analysis.
文摘以吲哚乙酸（IAA）为模板分子、乙腈为致孔剂,采用沉淀聚合法制备了其分子印迹聚合物,通过静态吸附实验研究了致孔剂乙腈的用量及4-乙烯基吡啶（4-VP）和丙烯酰胺（AA）两种不同性质的功能单体（M）对印迹聚合物吸附性能的影响。结果表明,以4-VP为M,乙腈的加入量分别为20、25、37.5和50 m L时,制备的印迹聚合物P1~P4均具有良好的分散性,且聚合物P4对IAA具有最显著的印迹效应（印迹因子IF=2.92）;而在同样条件下以AA为M的印迹聚合物的印迹效应较小（IF=1.73）。选择性实验表明,聚合物P4能够从吲哚羧酸同系物中选择性结合IAA。Scatchard分析表明,聚合物P4对IAA表现出两类结合位点,其高亲和力与低亲和力结合位点的平衡离解常数分别为Kd1=0.798 mmol/L和Kd2=7.76 mmol/L,对应的最大结合量分别为21.0和90.7μmol/g。
