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Molecular Marker Development in Post-genomic Era:Leveraging Multiple Resources for Marker Development in Cotton and Other Crops
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作者 KUMPATLA Siva P SHAH Manali R MUKHOPADHYAY Snehasis THOMPSON Steven A GREENE Thomas W 《棉花学报》 CSCD 北大核心 2008年第S1期29-,共1页
While the importance of molecular marker technology was realized more than two decades ago,high-throughput marker development came into vogue only after the availability of hundreds of thousands of sequences in public... While the importance of molecular marker technology was realized more than two decades ago,high-throughput marker development came into vogue only after the availability of hundreds of thousands of sequences in public databases.Many examples now exist where markers are being used routinely in breeding programs for marker-assisted selection(MAS) of traits of interest or marker assisted recovery of genome of interest.Genetic analysis with thousands to tens of thousands of markers is now possible due to the... 展开更多
关键词 SSR molecular marker development in Post-genomic Era
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盐渍条件下野大豆群体的遗传分化和生理适应:同工酶和随机扩增多态DNA研究 被引量:36
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作者 王洪新 胡志昂 +4 位作者 钟敏 陆文静 魏伟 恽锐 钱迎倩 《Acta Botanica Sinica》 CSCD 1997年第1期34-42,共9页
黄河三角洲野大豆(Glycine soja Sieb. et Zucc.)盐渍群体的耐盐性高于附近的正常群体。群体内个体间耐盐能力差别很大。盐渍群体有比最耐盐的栽培大豆(G.max (L.)Merr.)品种耐盐能力高得多的个体,也有对盐相当敏感的植株。同工酶分析... 黄河三角洲野大豆(Glycine soja Sieb. et Zucc.)盐渍群体的耐盐性高于附近的正常群体。群体内个体间耐盐能力差别很大。盐渍群体有比最耐盐的栽培大豆(G.max (L.)Merr.)品种耐盐能力高得多的个体,也有对盐相当敏感的植株。同工酶分析表明群体内高水半多态性,但酶谱与抗性没有相关性。盐渍与正常群体间的遗传一致性高达0.96。用改良的随机扩增多态DNA(RAPD)方法,10个引物扩增得出群体内多态位点百分数为68/188=0.36。看来,绝大多数位点与耐盐能力无关。上述资料说明,盐渍条件下野大豆自然群体的高度遗传多样性和发育变通性,可能足对盐胁迫强度随时随地变化的环境的适应。 展开更多
关键词 野生 大豆 耐盐性 同功酶 DNA 遗传分化
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