Molecular imprinting technique is a simple and efficient method for the preparation of polymer materials (i.e., molecularly imprinted polymers, MIPs) with tailor-made recognition sites for certain target molecules. Th...Molecular imprinting technique is a simple and efficient method for the preparation of polymer materials (i.e., molecularly imprinted polymers, MIPs) with tailor-made recognition sites for certain target molecules. The resulting MIPs have proven to be versatile synthetic receptors due to their high specific recognition ability, favorable mechanical, thermal and chemical stability, and ease of preparation. Recent years have witnessed significant progress in the synthesis and applications of MIPs. This review focus on the recent developments and advances in the preparation of MIPs via various controlled radical polymerization techniques.展开更多
Surface molecularly imprinted polymers （SMIPs） for selective adsorption of ampicillin sodium were synthesized using surface molecular imprinting technique with silica gel as a support. The physical and morphological...Surface molecularly imprinted polymers （SMIPs） for selective adsorption of ampicillin sodium were synthesized using surface molecular imprinting technique with silica gel as a support. The physical and morphological characteristics of the polymers were investigated by scanning electron microscope （SEM）, Fourier transform infrared spectroscopy （FT-IR）, thermogravimetric analysis （TGA）, elemental analysis and nitrogen adsorption-desorption test. The obtained results showed that the SMIPs displayed great adsorption capacity （13.5 lag/mg）, high recognition ability （the imprinted factor is 3.2） and good binding kinetics for ampicillin sodium. Finally, as solid phase extraction adsorbents, the SMIPs coupled with HPLC method were validated and applied for the enrichment, purification and determination of anapicillin sodium in real milk and blood samples. The averages of spiked accuracy ranged from 92.1% to 107.6%. The relative standard deviations of intra- and inter-day precisions were less than 4.6%. This study provides a new and promising method for enriching, extracting and determining ampicillin sodium in complex biological samples.展开更多
A new biomimetic bulk acoustic wave sensor based on molecularly imprinted microspheres (MUM) technique was described. The sensing materials were synthesized by precipitation polymerization. By using the Scatchard anal...A new biomimetic bulk acoustic wave sensor based on molecularly imprinted microspheres (MUM) technique was described. The sensing materials were synthesized by precipitation polymerization. By using the Scatchard analysis, the equilibrium dissociation constant K-D and the apparent maximum number Q(max) of the binding sites were calculated to be 3.70 mmol (.) L-1 and 9.11 mumol (.) g(-1), respectively. The sensor exhibited a sensitive response to the template compound (dipyridamole) in liquid phase with a detection Unfit of 2 x 10(-9) mol (.) L-1. The recoveries of the sensor were 95.1%-105.4%. Studies presented in this paper show that the stability of this sensor is excellent. The sensor has been applied successfully to the determination of dipyridamole in human urine.展开更多
Synthetic materials that can specifically recognize proteins will find wide application in many fields.In this report,bovine serum albumin was chosen as the template protein.Acrylamide and N,N＇-methylenebisacrylamide...Synthetic materials that can specifically recognize proteins will find wide application in many fields.In this report,bovine serum albumin was chosen as the template protein.Acrylamide and N,N＇-methylenebisacrylamide were employed as the functional and cross-linker monomers,respectively.Molecularly imprinted macroporous monolithic materials that can preferentially bind the template protein in an aqueous environment were prepared by combination of molecular imprinting technique and freezing/thawing preparation method.The resulted imprinted macroporous monolithic columns were evaluated by utilizing as stationary phase in high performance liquid chromatography and solid-phase extraction materials.The experimental results indicated that the imprinted macroporous monolithic column exhibited good recognition for template protein,as compared with the control protein（hemoglobin）,whereas the non-imprinted polymer（prepared under the same conditions except without addition template protein） had no selective properties.展开更多
基金National Natural Science Foundation of China (20744003, 20774044)Natural Science Foundation of Tianjin (06YFJMJC15100)A supporting program for New Century Excellent Talents (Ministry of Education) (NCET-07-0462).
文摘Molecular imprinting technique is a simple and efficient method for the preparation of polymer materials (i.e., molecularly imprinted polymers, MIPs) with tailor-made recognition sites for certain target molecules. The resulting MIPs have proven to be versatile synthetic receptors due to their high specific recognition ability, favorable mechanical, thermal and chemical stability, and ease of preparation. Recent years have witnessed significant progress in the synthesis and applications of MIPs. This review focus on the recent developments and advances in the preparation of MIPs via various controlled radical polymerization techniques.
基金financially supported by the National Natural Science Foundation of China (Nos. 81573391 and 81173024)the National Key Projects of China (No. 812277802)
文摘Surface molecularly imprinted polymers （SMIPs） for selective adsorption of ampicillin sodium were synthesized using surface molecular imprinting technique with silica gel as a support. The physical and morphological characteristics of the polymers were investigated by scanning electron microscope （SEM）, Fourier transform infrared spectroscopy （FT-IR）, thermogravimetric analysis （TGA）, elemental analysis and nitrogen adsorption-desorption test. The obtained results showed that the SMIPs displayed great adsorption capacity （13.5 lag/mg）, high recognition ability （the imprinted factor is 3.2） and good binding kinetics for ampicillin sodium. Finally, as solid phase extraction adsorbents, the SMIPs coupled with HPLC method were validated and applied for the enrichment, purification and determination of anapicillin sodium in real milk and blood samples. The averages of spiked accuracy ranged from 92.1% to 107.6%. The relative standard deviations of intra- and inter-day precisions were less than 4.6%. This study provides a new and promising method for enriching, extracting and determining ampicillin sodium in complex biological samples.
文摘A new biomimetic bulk acoustic wave sensor based on molecularly imprinted microspheres (MUM) technique was described. The sensing materials were synthesized by precipitation polymerization. By using the Scatchard analysis, the equilibrium dissociation constant K-D and the apparent maximum number Q(max) of the binding sites were calculated to be 3.70 mmol (.) L-1 and 9.11 mumol (.) g(-1), respectively. The sensor exhibited a sensitive response to the template compound (dipyridamole) in liquid phase with a detection Unfit of 2 x 10(-9) mol (.) L-1. The recoveries of the sensor were 95.1%-105.4%. Studies presented in this paper show that the stability of this sensor is excellent. The sensor has been applied successfully to the determination of dipyridamole in human urine.
基金supported by the National Natural Science Foundation of China(Nos21075089 and 20605024)Talents Fund of Tianjin University of Science and Technology(No20090424)
文摘Synthetic materials that can specifically recognize proteins will find wide application in many fields.In this report,bovine serum albumin was chosen as the template protein.Acrylamide and N,N＇-methylenebisacrylamide were employed as the functional and cross-linker monomers,respectively.Molecularly imprinted macroporous monolithic materials that can preferentially bind the template protein in an aqueous environment were prepared by combination of molecular imprinting technique and freezing/thawing preparation method.The resulted imprinted macroporous monolithic columns were evaluated by utilizing as stationary phase in high performance liquid chromatography and solid-phase extraction materials.The experimental results indicated that the imprinted macroporous monolithic column exhibited good recognition for template protein,as compared with the control protein（hemoglobin）,whereas the non-imprinted polymer（prepared under the same conditions except without addition template protein） had no selective properties.
