Phytochemical screening and antibacterial activities of Momordica charantia and Vernonia amygdalina extracts on some selected enteric isolates

Background:This research focuses on herbal medicine,an ancient healthcare practice,exploring the antibacterial attributes of fresh and dried leaf extracts from Momordica charantia(commonly known as Bitter melon)and Vernonia amygdalina(Bitter leaf).The study specifically investigates their effects on different bacterial strains associated with gastroenteritis.Methods:Four enteric bacterial isolates-Klebsiella pneumoniae,Salmonella typhi,Escherichia coli,and Proteus mirabilis-were obtained from the Medical Laboratory Unit at Babcock University Teaching Hospital in Ilishan-Remo,Ogun State.Phytochemical screening and antibacterial testing were conducted using standard biochemical techniques and the Punch-hole agar diffusion method,respectively.Results:Qualitative phytochemical screening of the plant extracts revealed the presence of flavonoids,glycosides,and saponin in both plants,excluding terpenoids.Alkaloids were identified only in Vernonia amygdalina.Despite these phytochemicals,neither plant displayed inhibitory effects on the tested bacterial isolates(Escherichia coli,Proteus mirabilis,Klebsiella pneumoniae,and Salmonella typhi)when tested individually or in combination.Intriguingly,combining the fresh and dried leaf extracts of Momordica charantia and Vernonia amygdalina with a standard drug resulted in smaller mean zone diameters of inhibition(Escherichia coli range:14 mm–16 mm,Proteus mirabilis range:31 mm–35 mm,Klebsiella pneumoniae range:13 mm–22 mm,and Salmonella typhi range:35 mm–38 mm)compared to the drug tested alone(16 mm–45 mm).Conclusion:Despite previous indications of antibacterial properties in various extracts of V.amygdalina and M.charantia leaves,our study presents contradictory results,prompting the need for further investigation despite the presence of significant phytochemicals.

Evaluation of Acute and Sub-Acute Oral Toxicities of Momordica foetida Schumach. (Cucurbitaceae) Leaves Methanol Extract in Wistar Rats

Momordica foetida is a plant widely used in tropical Africa to manage gastroenteric diseases. Previous studies demonstrated interesting antibacterial activity against human pathogenic bacteria. However, the security or toxicity of methanol leaf extract has not been determined yet. The aim of this study was to evaluate the acute and sub-acute toxicity of the leaf extract of Momordica foetida. In the acute toxicity study, a single oral dose of 5000 mg/kg body weight was administered to rats which were observed for 14 days in order to identify signs of toxicity or death. In the sub-acute toxicity, the animals were treated with 250, 500 and 1000 mg/kg of the extract for 28 consecutive days. Body weights and behavior were noted throughout the experiment. Upon treatment, blood and urine were collected for hematological and biochemical analysis. Liver, lungs, heart, kidneys, testes and ovaries were analyzed for relative weights and histopathology. The acute toxicity study of M. foetida leaf extract revealed no signs of toxicity related to the treatment, indicating that the median-lethal-dose (LD50) value is greater than 5000 mg/Kg of body weight. In the sub-acute toxicity assay, the extract did not affect the general behavior of animals, meanwhile, it led to a significant increase in the levels of red blood cells, platelets, hemoglobin, granulocytes and Mid-Cells (MIDs). Biochemical parameters showed an increase in total cholesterol, HDL cholesterol, serum urea, serum and urinary glucose and a decrease in urinary proteins, serum creatinine, urinary urea levels, serum activities of AST, ALT and proteins levels, as well as increases in lung, spleen and ovaries relative weight were noticed, all compared to control animals. Histological analysis revealed a normal architecture of kidneys, liver, heart, lung, ovaries and testes. This study provides valuable data on the safety of per os administration of Momordica foetida leaf methanol extract that could be very useful for future assays.

Chemical Composition and Use of Momordica charantia L. and Hyptis spicigera Lam. Extracts as Mosquito Larvicides and Insect Growth Regulators against Malarial Vector, Anopheles gambiae Giles

Background: Mosquitoes are responsible of numerous vector-borne diseases and among these diseases is malaria which takes away lives of thousands of people, especially children of under five, particularly in Africa. To reduce the mortality and economic burdens of this deadly disease, synthetic insecticide has been of use to control its main vector, Anopheles gambiae. Due to adverse effects caused by these conventional products on non-target organisms and the environment, plants have been of first choice as they proved their effectiveness against mosquitoes and are said to be eco-friendly and relatively safer. Therefore, the aim of this study was to screen the phytochemicals and determine the larvicidal and adult emergence exhibitory activities of Momordica charantia and Hyptis spicigera leaves extracts against Anopheles gambiae larvae. Methods: The WHO standard protocol for larvicidal activity and IGRs was followed up and concentrations from 500 - 2000 ppm for larvicidal and 500 - 1500 ppm for IGRs were set up. Results: There was the presence of alkaloids, flavonoids, tannins, phenols, steroids, terpenoids and oils in Momordica charantia against alkaloids, taponins, tannins, phenols, steroids, terpenoids and oils in Hyptis spicigera. Methanol crude extract registered the lowest LC50 value of 270.6 ppm followed by ethyl acetate and hexane fractions recorded the LC50 of 742.1 and 756 ppm, respectively for larvicidal activity of Momordica charantia against LC50 of 760 and 867.5 ppm for hexane fraction and methanol crude extract, respectively for Hyptis spicigera. Methanol crude extract and hexane fraction of both plants as well as the ethyl acetate fraction of M. charantia registered greater emergence inhibition with IE50 values of 590.2, 842.3 and 982 ppm for methanol crude extract, hexane and ethyl acetate fractions of M. charantia, respectively against 901.7 and 873.2 for methanol crude extract and hexane fraction of H. spicigera, respectively. Conclusion: The toxicity and adult emergence inhibition may be associated with constituents in both plants that interfere with the normal neuronal, respiratory and endocrine systems functions.

Biosorptive Removal of Lead Ions from Aqeuous Stream by Using Leaves, Stems and Roots of Momordica charantia Biomass

Water pollution is one of the most important issues of the 21st century. It takes place when the pollutants are being entered into a water reservoir without any treatment. Heavy metals are one of the major harmful pollutants that exist in the water;therefore, it is necessary to remove these toxic metals to keep our environment safe. Biosorption is an ecofriendly and economical technique for the elimination of these toxic metals from polluted water. In this research work roots, stems, and leaves of Momordica charantia (Bitter gourd) were used as biosorbent for the elimination of Pb (II) ions from aqueous solution. Many different parameters such as metal ion solution pH, biomass dosage, initial metal ions concentration and contact time were optimized in the batch experiments. The calculated results revealed that biosorption of Pb (II) was maximum at solution pH 5, biosorbent dosage of 0.1 g and 100 ppm of initial metal ions concentration within 240 minutes of contact time. M. charantia’s leaves showed the highest level of lead biosorption capacity (47.62%), followed by stems (42.36%) and roots (38.47%). The Freundlich isotherm and pseudo-second order kinetics model fitted well for the analytical data. The results indicated that Momordica charantia is an effective biosorbent for Pb (II) ions elimination from wastewater.

Food prospects and nutraceutical attributes of Momordica species:A potential tropical bioresources–A review

Plants with potential therapeutic values have been used from time immemorial to cure various ailments and infectious diseases.Of late,scientific evidences have been provided on the potential therapeutic agent exhibited by certain traditionally used vegetable extracts.The importance of wild edible plants may be traced to antiquity but systemic studies are recent.All the Momordica species have been consumed as vegetable and traditionally used for various disorders.The whole plant parts are ascribed to possess the anti-diabetic effect in traditional medicinal system.The active constituents of Momordica plant parts were cucurbitane type triterpenoids,phenolics,glycosides,and several kinds of peptides including Momordica anti-HIV protein(MAP 30).Recent reports revealed the presence of several kinds of cucurbitane type triterpenoids in leaf,stem and fruits of Momordica species having several pharmacological activities.There is lack of scientific information available on the wild species which also having several bioactive components with potential activities.So the present review compares and highlights the current knowledge of the nutritional value,phytochemistry and physiological effects of wild species with known variety.

CPPU对苦瓜(Momordica charantia L.)单性结实的诱导作用及其机理的研究

以中熟南山大白苦瓜为材料,研究了外源细胞分裂素活性物质CPPU对苦瓜单性结实的诱导作用和CPPU对苦瓜果实内源激素水平的调控,50mg L-1CPPU于花前3d处理,可引起苦瓜子房不正常开花(如花瓣绿色或边缘为绿色,花瓣小、厚、硬、粗糙;柱头干燥、光滑、绿色等)或盲花,使苦瓜子房不能正常受粉或受粉失败,从而形成无籽的苦瓜果实;CPPU处理提高了子房内源GA、IAA水平,是CPPU处理引起受粉失败或不能正常受粉的子房仍然能正常座果并生长发育形成无籽果实的重要原因,同时CPPU处理对内源CTK水平具有抑制作用,对其机制的探讨具有十分重要的意义。

苦瓜(Momordica CharantiaL.)MADS-box基因BAG启动子的克隆分析及表达载体的构建

作者提取苦瓜基因组总DNA,构建了DNA步移文库,并根据已克隆并公布的苦瓜MADS box基因BAG的基因序列设计引物,通过染色体步移技术克隆出BAG基因起始密码子上游调控序列BAGP.对BAGP的鉴定和分析表明其具备大多数高等植物启动子的保守元件,预测它对BAG基因的表达具有一定的作用.为鉴定BAG基因的基本启动子元件,将基因5′侧翼序列做缺失片段分析,利用PCR方法从BAGP中得到3个大小不等两端带有HindⅢ,BamHⅠ酶切位点的片段BAGP1,BAGP2和BAGP3,定向插入载体pMGFP4(pBI221改建,报告基因为GFP)中,取代原有的CaMV35S启动子,构建了由驱动报告基因GFP的植物表达载体BAGPV1,BAGPV2和BAGPV3.

A comparative study on antioxidant potentials,inhibitory activities against key enzymes related to metabolic syndrome,and anti-inflammatory activity of leaf extract from different Momordica species

Momordica species are vegetable crops widely distributed in warmer regions of the world.In this work,we describe the antioxidant,enzyme inhibitory and anti-inflammatory effects of the leaves from three different species of Momordica.The present investigation was initially carried out to explore the possible link between antioxidant,enzyme inhibitory property of Momordica leaf extract and their total phenolic and flavonoid contents.The anti-inflammatory activity was tested by using carrageenan-induced paw edema.Our results illustrated an enhanced antioxidant power of wild species comparable with a commercial variety.In addition,the leaf extract of M.dioica(200 mg/kg)presented a significant anti-inflammatory activity toward carrageenan-induced paw edema in Wistar rats in comparison to indomethacin(10 mg/kg).The contents of flavonoids and total phenolic compounds could be correlated with the antioxidant and enzymes inhibition activities.The major bioactive compounds of phenolic acids and flavonoids such as gallic acid,chlorogenic acid,caffeic acid,ferulic acid,ellagic acid,catechin,rutin,and quercetin were identified.Our findings suggest that wild Momordica species contains higher potential antioxidant and anti-inflammatory activities than a commercial variety does,which could be tested as drug candidates against oxidative and inflammation-related pathological processes.©2014 Beijing Academy of Food Sciences.Production and hosting by Elsevier B.V.All rights reserved.

Antioxidant compounds and capacities of Gac (Momordica cochinchinensis Spreng) fruits

Objective: To identify and determine the composition of antioxidant compounds, and to evaluate the antioxidant abilities of Gac fruit parts(peel, pulp, seed and aril) grown in Malaysia. Methods: LC-MS/MS was used for identification of antioxidant compounds and UV-Vis for estimation of the contents of phenolics, flavonoids, and carotenoids. Lycopene and β-carotene were quantified using high-performance liquid chromatography. DPPH(2, 2-diphenyl-1-picrylhydrazyl) and ferric reducing antioxidant power assays were employed to evaluate antioxidant capacities. Results: Phytochemicals were found amongst all the fruit parts. Notably, significant amounts of carotenoids [(107.4 ± 4.5),(85.7 ± 4.4),(110.6 ± 2.1) mg/100 g dry weight(DW)], and relatively high levels of both phenolics [(27.3 ± 1.7),(28.9 ± 2.4),(30.8 ± 2.7) mg/100 g DW] and flavonoids [(38.1 ± 2.2),(8.8 ± 1.3),(24.5 ± 3.3) mg/100 g DW] were found in the fruit's peel, pulp and aril, respectively. Seed part also showed a relatively high level of flavonoids [(18.1 ± 2.3) mg/100 g DW]. Lycopene and β-carotene were found to be significantly high(P < 0.05) in aril [(579.3 ± 22.7) and(621.0 ± 35.0) μg/g DW], followed by peel [(51.0 ± 7.5) and(210.0 ± 12.5) μg/g DW] and pulp [(37.6 ± 10.9) and(205.6 ± 22.1) μg/g DW)]. Antioxidant assays revealed that aril possessed the highest scavenging activity(IC_(50) = 865 μg/mL), while the peel possessed the highest ferric reducing power of 140 μmol FeSO4/μg. Conclusions: The current results demonstrate that Gac fruit grown in Malaysia is a rich source of phytochemicals, especially carotenoids, and possesses antioxidant activities. Thus, such findings suggest Gac fruit as a source of an antioxidant plant.

Comparative study between the effect of <i>Momordica charantia</i>(wild and hybrid variety) on hypoglycemic and hypolipidemic activity of alloxan induced type 2 diabetic long-evans rats

This study was undertaken to evaluate the hypoglycemic and hypolipidemic effect of Momordica charantia (wild and hybrid variety) powder on alloxan induced type 2 diabetic male Long-Evans rats. Oral feeding of the M. charantia powder slightly decreased serum total cholesterol, triglyceride levels and LDL-cholesterol compared with wild, hybrid and standard drug. M. charantia wild variety showed more significant (p M. charantia did not show any significant effect on HDL-cholesterol and liver glycogen. Thus, results of the study prove that the wild variety of M. charantia fruit have potent antidiabetic and antilipidemic property.

Attenuation of experimentally induced diabetic neuropathy in association with reduced oxidative-nitrosative stress by chronic administration of <i>Momordica charantia</i>

Momordica Charantia (MC) is one of the most famous traditional plant worldwide, used for the treatment of diabetes and its complications. In the present study possible protective effect of MC in Streptozotocin (STZ) induced diabetic neuropathy in mice was evaluated. STZ induced diabetic mice were orally administered MC at various doses (200 - 800 mg/kg) for six weeks. Diabetes induced neuropathic pain was assessed by hot plate test, formalin test and tail flick test at the beginning and end of the study. Serum TBARS, NO and SOD levels were estimated at the end of the study as the markers of oxidative-nitrosative stress. Rotarod test was employed to assess the effect of treatment on motor coordination. The results showed that STZ induced diabetes significantly decreased the pain threshold as was indicated by increased flinching in formalin test and decreased withdrawal latency in hot plate and tail flick tests. Oxidative-nitrosative stress was significantly increased in diabetic animals. Chronic administration of MC significantly attenuated diabetes induced increase in flinches and decrease in withdrawal latency without impacting sensory and motor functions. MC administration also exhibited dose dependant reduction of hyperglycemia and serum TBARS, NO and SOD levels in diabetic mice. The results suggest that long term use of MC protects against diabetes induced neuropathy in association with attenuation of hyperglycemia and oxidative-nitrosative stress.

Fusarium Wilt Resistance Identification and Genetic Analysis of Momordica charantia L. Germplasm

Fusarium wilt seriously affects the yield and quality of Momordica charantia L.Breeding resistant varieties is the most economical and effective measure to control Fusarium wilt in M.charantia L.This study had comprehensively conducted pathogen identification of Fusarium wilt,resistance evaluation of M.charantia L.germplasm to Fusarium wilt,and genetic analysis of Fusarium wilt resistance in M.charantia L.,so as to provide reference for breeding resistant varieties and accelerating the breeding process.

Proximate and Major Minerals Components of the Edible Pulp of <i>Momordica charantia</i>Fruit

Momordica charantia (Cucurbitaceae), is a plant commonly used in traditional medicine in Senegal whose fruits are weakly consumed by the population. However, no data exists on the nutritional intake of the latter. This work aims to determine the physicochemical and biochemical characteristics of the pulp of Momordica charantia. The study focused on two lots of fruits from two geographically different regions of Senegal (Dakar and Fatick). The biochemical analyses of the pulp showed average values of protein from 2.39 ± 0.01 g/100 g to 3.19 ± 0.01 g/100 g;reducing sugar from 4.22 ± 0.10 g/100 g to 3.55 ± 0.10 g/100 g;totals sugar from 4.43 ± 0.02 g/100 g to 3.38 ± 0.58 g/100 g. The average contents of polyphenol and flavonoids were respectively from 1.91 ± 0.04 g/100 g to 1.04 ± 0.09 g/100 g and 32.59 ± 0.04 mg/100 g and 20.75 ± 1.19 mg/100 g. According to these results, the fruit of M. charantia may be considered as rich in antioxidant. At the opposite, it is very weak in mineral elements, such as calcium and potassium (1.01 mg/100 g;3.38 mg/100 g). Meanwhile, the seeds content 24% of lipids. Thus, investigations should be done on its biological proprieties, physical and biochemical components.

Analysis of <i>Momordica charantia</i>Polysaccharide Components and Their Effects on KA-Induced Oxidative Stress and Neuronal Loss in the Hippocampus of Epileptic Rats

Polysaccharides of Momordica charantia have been reported to be the primary bioactive components that confer its antioxidant activity. To some degree, antioxidants may provide beneficial effects on neuronal damage induced by seizures. However, the protective effects of Momordica charantia polysaccharides (MCPs) on seizures remain unclear. In this study, our purpose was to investigate the effects of MCPs on oxidative stress and neurodegeneration in an experimental Kainic acid (KA)-induced rat seizure model. MCPs treatments decreased the level of malondialdehyde (MDA) and increased the activities of superoxide dismutase (SOD) and catalase (CAT) in the hippocampus of the KA-induced animal model. Moreover, MCPs significantly attenuated KA-induced neuronal loss in the CA1 and CA3 hippocampal regions. Based on these results, MCPs exert neuroprotective effects by attenuating KA-induced neuronal damage in the brain through their free radical scavenging activities.

Identification of a soluble phosphatidate phosphohydrolase in the developing cotyledons of <i>Momordica</i><i>charantia</i>

Phosphatidate phosphatase (EC 3.1.3.4), PAP, catalyzes the dephosphorylation of phosphatidate (PtdOH) to form diacylglycerol. In eukaryotes, PAP driven reaction is the committed step in the synthesis of triacylglycerol. A Mg2+ independent PAP activity was identified in the soluble extract of Momordica charantia cotyledons undergoing maturation. While the microsomal fraction of the extract gave only 10% of the PAP activity, the remaining 90% of the activity was associated with the soluble fraction. At pH 3.0, the soluble PAP was bound to S column and eluted with glycine-HCl buffer containing high salt. The pH and temperature optima of the PAP activity were 6.0 and 53℃, respectively. Under optimum assay condition, the Vmax and Km for dioleoyl phosphatidic acid were 1.89 ηkat/mg of protein and 142 μM, respectively. For the synthetic substrate, ρ-nitrophenylphosphate, ρ- NPP, the Vmax and Km were 10.4 ηkat/mg of protein and 107 μM, respectively. The inclusion of Mg2+ and β-mercaptoethanol into the reaction mix did not change the enzyme activity nor did the addition of N-ethylmaleimide and phenylglyoxal, which indicates that cysteine and arginine are not involved in catalysis of PtdOH. The addition of Mg2+ up to 10 mM also did not change the level of PAP activity. Triton X-100, however, inhibited the activity. This is the first documented case of an in vitro PAP activity in the developing cotyledons of Momordica charantia. The PAP described here could serve as a model for lipin-1 or lipin-2 in humans. Mutations in these genes lead to acute myoglobinuria in human infants.

Clinicopathological Study on the Effects of <i>Momordica charantia</i>on Streptozotocin-Induced Diabetic Wistar Rats

Momordica charantia (MC) a traditional medicine used for the treatment of many disorders was fed to streptozotocin (STZ)-induced diabetic male Wistar rats at 2, 5 and 10% of the standard diet. Rats were then observed for 12 weeks before sacrificed. Immediately, tissues from liver, kidney and pancreas were taken for histopathological examination. Serum samples were analyzed to evaluate liver, kidney and pancreatic functions. In addition, blood samples were collected to analyze hematology parameters. The results of the present study indicate that oral doses of MC at 5% and 10% of the daily diet resulted in alleviation of the pancreatic, hepatic and renal dysfunction induced by diabetes. The improved pancreatic, hepatic and renal functions were judged by histopathological, hematological and serobiochemical parameters. In conclusion, the MC fruits may be used as an antidiabetic herbal medicine.

Phylogenetic analysis of Endophytes from Bitter Melon (<i>Momordica charantia</i>) in Guangdong Province

This study aims to isolate and identify taxonomic characterization of endophytic fungi from bitter melon in Guangdong province, China. A total of 1172 endophytic fungi are isolated from roots, stems, leaves, flowers, and fruits of healthy plants, and they are classified to 49 taxa based on morphological and molecular features. The results show that endophytic fungi from bitter melon plants exhibit high biodiversity. Arthrinium aureum, A. marii, A. sphaerospermum, Corynascus verrucosus, Curvularia borreriae and C. protuberate have not been recorded in any plants in China. Basidiomycetous endophytes, such as Ceratobasidium sp., and C. cornigerum, are reported in this study for the first time in bitter melon. According to the results of phylogenetic analysis, the rDNA ITS (Internal Transcribed Spacer) sequences can successfully separate species, such as F. solani, F. kyushuense, C. verrucosus, C. globosum, E. rostratum, C. brachyspora, C. verruculosus, C. affinis, P. bougainvilleicola, P. longicolla, P. glabrae, P. verruculosum, P. oxalicum, P. citrinum, P. chermesinum, P. glomerata, A. fumigatus and A. japonicas. Some isolates belonging to A. alternata, C. gloeosporioides, C. cladosporioides, C. brasiliense, C. convolutum, F. proliferatum, F. oxysporum, F. verticillioides, F. equiseti, F. camptoceras and Xylaria, however, require the analysis of others molecular markers to provide better taxonomic resolution. Molecular analyses of rDNA ITS sequences are useful for identification and classification of endophytes of bitter melon, but it is desirable to consider an integrated approach, such as molecular phylogeny, host, colony growth rate, symptoms, and shape of conidia.

Cucurbitane-Type Triterpene Glycosides from Momordica charantia and Their α-Glucosidase Inhibitory Activities

Ten cucurbitane-type triterpene glycosides,including five new compounds named charantosides H(1),J(2),K(3),momor-characoside A(4),goyaglycoside-l(5),and five known compounds(6-10),were isolated from the EtOAc extract of Momor-dica charantia fruits.The chemical structures of these compounds were identified by 1D and 2D NMR and HRESIMS spectroscopic analyses.Configurations of new compounds were determined by ROESY correlations and comparison of their 13C NMR data with literature reported values.All compounds were evaluated for their inhibition againstα-glucosidase,in which compounds 2,5,7,8,9 showed moderate inhibitory activities with IC50 values ranging from 28.40 to 63.26μM comparing with the positive control(acarbose,IC5087.65±6.51μM).

Field Evaluation on Agronomic Characteristics of Newly Introduced Bitter Gourd(Momordica charantia L.)Accessions in Thua Thien Hue Province,Vietnam

The main objective of this study was to evaluate ability of growth and yield of introduced bitter gourd accessions in winter-spring 2016-2017 in Thua Thien Hue province.A total of seven accessions were used in this study.Of these,six accessions were provided by the World Vegetable Center(AVRDC),namely,AVRDC 1329,AVRDC 1330,AVRDC 1331,AVRDC 1333,AVRDC 1334,AVRDC 1335 and one Vietnam local accession(control)from Dien Hai commune,Phong Dien district,Thua Thien Hue province.The results showed that experiment accessions can grow under Thua Thien Hue conditions.AVRDC 1329,AVRDC 1330,and AVRDC 1335 were considered as displaying good growth and development ability.Of those,AVRDC 1330 was the most suitable to consumer as regards to appearance and bitter taste.AVRDC 1330,the control check and AVRDC 1331 had the high actual yield with 16.57,10.65 and 7.88 tons/ha,respectively,and these two introduced accessions can be used for breeding and cultivation under local condition.

Impact of Extraction on Biochemical Properties and Antioxidant Potential of Momordica charantia L. Seeds’ Oil

In this study, the influence of provenance and extraction methods on the physicochemical properties and the antioxidant potential of M. charantia seeds oil were evaluated. The oil is obtained on the one hand by cold extraction with hexane and on the other hand by hot extraction with soxhlet. The results obtained show that the extraction yield is significantly impacted by the extraction methods and the origin of the seeds. In addition, the soxhlet extraction gives a higher oil yield (32.07 ± 0.01). Cold extraction has made it possible to obtain oils with less attenuated physicochemical characteristics. Indeed, the acid numbers are high in the oils extracted by soxhlet (5.92 ± 0.25;4.25 ± 0.62 and 13.86 ± 0.83) than in those cold extracted with very low peroxide for all the oils obtained. On the other hand, the iodine and refractive indices are high in oils obtained cold (91.58 ± 0.85;100.74 ± 0.03 and 102.08 ± 0.28) (1.53 ± 0.01;1.52 ± 0.01 and 1.52 ± 0.01) with low saponification indices. The polyphenol concentrations and the anti-free radical activity are higher with the oils obtained cold (0.086 ± 0.001;0.08 ± 0.000 and 0.09 ± 0.01 mgEAG/g of oil) and (DPPH) (55.75% ± 1.16%;55.03% ± 0.72% and 56.35% ± 0.45%). The color parameters (L*, a* and b*) of the different oils extracted also vary depending on the extraction method used. Principal Component Analysis (PCA) and correlation analysis were performed on the physicochemical properties and the antioxidant potential of the extracted oils. Therefore, the results suggest cold extraction to obtain a good quality and oxidation resistant oil.