It is difficult to extract beneficial components from Monascus pigments due to their complex composition. The low purity of Monascus pigment preparations limits their further application in research and production. Th...It is difficult to extract beneficial components from Monascus pigments due to their complex composition. The low purity of Monascus pigment preparations limits their further application in research and production. Therefore, this study was conducted to optimize the conditions for extraction and isolation of Monascus pigments from red yeast rice, to improve the purity of Monascus pigment preparations. Three fractions were isolated using column chromatography from red yeast rice, they were red, orange and yellow respectively. Then, the three fractions were analyzed for composition and purity using thin layer chromatography and liquid chromatography-mass spectrometry(LC-MS). The chromatograms revealed that the yellow and orange fractions had complex compositions that were difficult to be separated, while the red fraction consisted of fewer components. Finally, a single component was isolated from the red fraction using a thin layer chromatography plate, identified as monascorubramine according to its molecular mass.展开更多
Synthetic dyes in the aqueous media have been commonly used for textile dyeing, resulting in resource and environmental pressure arising from consumption of water and environmentally unfriendly chemicals. In this stud...Synthetic dyes in the aqueous media have been commonly used for textile dyeing, resulting in resource and environmental pressure arising from consumption of water and environmentally unfriendly chemicals. In this study, an eco-friendly process of dyeing polyamide 6, 6(PA66) fabrics with natural Monascus pigments in decamethylcyclopentasiloxane(D5) solvent has been developed to minimize water consumption and effluent generation. The influence of processing parameters including dyeing temperature, dyebath pH and dyeing time on dyeing effects was explored. It was shown that color strength and color fastness of the samples dyed in D5 media were higher than those of the samples dyed in the aqueous media. Moreover, PA66 fabrics exhibited the highest color strength, good color fastness and a bacteriostatic rate of 53.6% against Staphylococcus aureus when it was dyed at pH of 3.5 and temperature of 80 ℃ for 30 min.展开更多
采用组织块分离法首次从药用植物地黄中分离出一株产红色素内生真菌RJL03,根据形态学特征和r DNA ITS1-5.8S-ITS2序列分析的手段鉴定为Monascus sanguineus。采用响应面法(RSM)对菌株RJL03中红色素提取工艺条件进行研究。在单因素实验...采用组织块分离法首次从药用植物地黄中分离出一株产红色素内生真菌RJL03,根据形态学特征和r DNA ITS1-5.8S-ITS2序列分析的手段鉴定为Monascus sanguineus。采用响应面法(RSM)对菌株RJL03中红色素提取工艺条件进行研究。在单因素实验的基础上,以提取液OD值为指标,利用Box-Behnken中心组合实验设计原理(BBD)优化菌株中红色素的有机溶剂超声波辅助浸提(UAE)工艺。结果显示,该色素提取液在515 nm处有最大吸收波长,最佳提取工艺为温度70℃、提取时间34 min、乙醇浓度50%。在此条件下提取菌株中的红色素,得到的提取液OD值为2.587,可比优化前提高约1.5倍。展开更多
In general,productions of natural pigment in submerged microorganism culture were much less than that in solid-state fermentation,because the solid-state culture can provide a support carrier for the mycelium. To impr...In general,productions of natural pigment in submerged microorganism culture were much less than that in solid-state fermentation,because the solid-state culture can provide a support carrier for the mycelium. To improve natural pigment production,the cultivation of Monascus purpureus in submerged encapsulated cell was investigated. Monascus purpureus immobilized in polyelectrolyte complex(PEC) microcapsules,which were pre-pared by sodium cellulose sulphate(NaCS) and poly-dimethyl-diallyl-ammonium chloride(PDMDAAC),was a good substitute for submerged cell culture because it mimicked the solid-state environment. The repeated-batch process with encapsulated cells was studied in flasks and a bubble column. The results indicated that the bubble column was more suitable for the encapsulation culture than the shaking flasks because of its good mass transfer performance and minor shear stress on cells. Owing to the protection of the microcapsule's membrane,Monascus purpureus in microcapsules increased approximately three times over that in free cell culture with negligible cell leakage to the medium. The pigment production in the bubble column finally reached 3.82(OD500) ,which was two times higher than in free cell culture. In addition,the duration of each batch was shortened to 15% of that in free cell culture.展开更多
[Objective] This study was conducted to optimize rice making process and improve the content of Monascus pigment. [Method]Selecting water addition( A),sterilization temperature( B),sterilization time( C),soaking time(...[Objective] This study was conducted to optimize rice making process and improve the content of Monascus pigment. [Method]Selecting water addition( A),sterilization temperature( B),sterilization time( C),soaking time( D) as experimental factors,L_9( 3~4) orthogonal test was carried out to optimize rice making process. [Result] The four factors all had very significant effect on the yield of Monascus pigment,the optimal combination was A_1B_2C_2D_2,namely water addition of 30 ml,sterilization temperature at 121 ℃,sterilization time of 23 min,and soaking time of 24 h. The level of Monascus reached 2 884 U/g under this condition. [Conclusion] The study has great practical significance to Monascus rice production enterprises.展开更多
[Objective] The aim was to study the fermentation technology of monascus pigment of monascus strains JF-02. [Method] Single factor experiment was carried out to study the influence of temperature, initial pH, culture ...[Objective] The aim was to study the fermentation technology of monascus pigment of monascus strains JF-02. [Method] Single factor experiment was carried out to study the influence of temperature, initial pH, culture time, different agricultural byproduct, and nitrogen source on monascus pigment in fermentation solution. Meanwhile, orthogonal experiment was conducted to get the optimal culture medium and cultivation condition. [Result] The optimal gene in the pigment of monascus pigment was 200 g/L of rice, 30 g/L of sweet potato powder, 10 g/L of glucose, 15 g/L of monosodium glutamate, 0.1% of zinc sulfate, and 0.1% of magnesium sulfate. The optimal culture condition was 30 ℃ and initial pH was 6.0. Fermentation time was 72 h, but when 24-L fermentation pot was used, culture time can last to 84 h. [Conclusion] The study provided theoretical basis for the development and application of monascus strains.展开更多
文摘It is difficult to extract beneficial components from Monascus pigments due to their complex composition. The low purity of Monascus pigment preparations limits their further application in research and production. Therefore, this study was conducted to optimize the conditions for extraction and isolation of Monascus pigments from red yeast rice, to improve the purity of Monascus pigment preparations. Three fractions were isolated using column chromatography from red yeast rice, they were red, orange and yellow respectively. Then, the three fractions were analyzed for composition and purity using thin layer chromatography and liquid chromatography-mass spectrometry(LC-MS). The chromatograms revealed that the yellow and orange fractions had complex compositions that were difficult to be separated, while the red fraction consisted of fewer components. Finally, a single component was isolated from the red fraction using a thin layer chromatography plate, identified as monascorubramine according to its molecular mass.
基金Fujian Provincial Department of Education,China(No.JAT190528)Quanzhou Science and Technology Project,China(No.2019C014R)。
文摘Synthetic dyes in the aqueous media have been commonly used for textile dyeing, resulting in resource and environmental pressure arising from consumption of water and environmentally unfriendly chemicals. In this study, an eco-friendly process of dyeing polyamide 6, 6(PA66) fabrics with natural Monascus pigments in decamethylcyclopentasiloxane(D5) solvent has been developed to minimize water consumption and effluent generation. The influence of processing parameters including dyeing temperature, dyebath pH and dyeing time on dyeing effects was explored. It was shown that color strength and color fastness of the samples dyed in D5 media were higher than those of the samples dyed in the aqueous media. Moreover, PA66 fabrics exhibited the highest color strength, good color fastness and a bacteriostatic rate of 53.6% against Staphylococcus aureus when it was dyed at pH of 3.5 and temperature of 80 ℃ for 30 min.
基金Supported by the National Basic Research Program of China（2007CB707805） the National Natural Science Foundation of China（20876139）
文摘In general,productions of natural pigment in submerged microorganism culture were much less than that in solid-state fermentation,because the solid-state culture can provide a support carrier for the mycelium. To improve natural pigment production,the cultivation of Monascus purpureus in submerged encapsulated cell was investigated. Monascus purpureus immobilized in polyelectrolyte complex(PEC) microcapsules,which were pre-pared by sodium cellulose sulphate(NaCS) and poly-dimethyl-diallyl-ammonium chloride(PDMDAAC),was a good substitute for submerged cell culture because it mimicked the solid-state environment. The repeated-batch process with encapsulated cells was studied in flasks and a bubble column. The results indicated that the bubble column was more suitable for the encapsulation culture than the shaking flasks because of its good mass transfer performance and minor shear stress on cells. Owing to the protection of the microcapsule's membrane,Monascus purpureus in microcapsules increased approximately three times over that in free cell culture with negligible cell leakage to the medium. The pigment production in the bubble column finally reached 3.82(OD500) ,which was two times higher than in free cell culture. In addition,the duration of each batch was shortened to 15% of that in free cell culture.
基金Supported by College Students’Science and Technology Innovation Activity Program(2014R461005)
文摘[Objective] This study was conducted to optimize rice making process and improve the content of Monascus pigment. [Method]Selecting water addition( A),sterilization temperature( B),sterilization time( C),soaking time( D) as experimental factors,L_9( 3~4) orthogonal test was carried out to optimize rice making process. [Result] The four factors all had very significant effect on the yield of Monascus pigment,the optimal combination was A_1B_2C_2D_2,namely water addition of 30 ml,sterilization temperature at 121 ℃,sterilization time of 23 min,and soaking time of 24 h. The level of Monascus reached 2 884 U/g under this condition. [Conclusion] The study has great practical significance to Monascus rice production enterprises.
基金Supported by Zhenjiang Science and Technology Plan Foundation in Jiangsu Province(NY2008047)
文摘[Objective] The aim was to study the fermentation technology of monascus pigment of monascus strains JF-02. [Method] Single factor experiment was carried out to study the influence of temperature, initial pH, culture time, different agricultural byproduct, and nitrogen source on monascus pigment in fermentation solution. Meanwhile, orthogonal experiment was conducted to get the optimal culture medium and cultivation condition. [Result] The optimal gene in the pigment of monascus pigment was 200 g/L of rice, 30 g/L of sweet potato powder, 10 g/L of glucose, 15 g/L of monosodium glutamate, 0.1% of zinc sulfate, and 0.1% of magnesium sulfate. The optimal culture condition was 30 ℃ and initial pH was 6.0. Fermentation time was 72 h, but when 24-L fermentation pot was used, culture time can last to 84 h. [Conclusion] The study provided theoretical basis for the development and application of monascus strains.
