In the present study we studied the genetic structure of five Chinese sheep populations of Hu sheep, Tong sheep, Small-tailed Han sheep, Tan sheep, and Wadi （WD） sheep using 15 microsatellite loci. The results showe...In the present study we studied the genetic structure of five Chinese sheep populations of Hu sheep, Tong sheep, Small-tailed Han sheep, Tan sheep, and Wadi （WD） sheep using 15 microsatellite loci. The results showed that the FIT, FST, and FIS statistics computed for the complete dataset had the following values, 0.523±0.140, 0.363±0.131 and 0.263±0.092, respectively. All loci were significantly contributed to the genetic differentiation among population （P0.001）. There is no relationship between the scatter of pairwise FST geographical distance points as geographical distance increases between the five populations. Membership probabilities and genetic structure of sheep populations were estimated when K=2, the populations were classified into Hu, Tong, Han and WD, and Tan sheep group. However, when K=3, the populations were classified into Hu and Tong, Han and WD, and Tan sheep group. The findings supported the previous literatures that these populations are originated on different time stages from the primogenitor population and communicated genetically with each other by natural and artificial selection in different ecological environment.展开更多
【目的】基于PI3K/Akt通路研究含凝血酶敏感素基序的去解联金属蛋白酶1(a disintegrin and metalloproteinase with thrombospondin motifs 1,ADAMTS1)在产双胎和单胎蒙古羊发情周期不同阶段卵巢内的表达规律,探究ADAMTS1基因影响蒙古...【目的】基于PI3K/Akt通路研究含凝血酶敏感素基序的去解联金属蛋白酶1(a disintegrin and metalloproteinase with thrombospondin motifs 1,ADAMTS1)在产双胎和单胎蒙古羊发情周期不同阶段卵巢内的表达规律,探究ADAMTS1基因影响蒙古羊繁殖性状的作用机制。【方法】选取经产2次的双胎和单胎蒙古羊共40只,自然发情,确定母羊发情期和间情期,分为双胎发情期、双胎间情期、单胎发情期和单胎间情期组,每组各10只羊。利用注射器抽取2~3岁蒙古羊颗粒细胞,分为对照组和试验组,对照组不添加药物,试验组添加15μmol/L LY294002处理颗粒细胞,分别在24、48和72 h测定细胞活力。通过实时荧光定量PCR技术检测母羊卵巢组织和颗粒细胞内ADAMTS1、PI3K、PTEN、Akt、RPS6、Bcl-2和BAX基因的相对表达量;通过Western blotting技术检测卵巢和颗粒细胞内ADAMTS1、PI3K和Akt蛋白的表达丰度。【结果】实时荧光定量PCR结果显示,双胎发情期组ADAMTS1、Bcl-2基因表达量均显著高于其余各组(P<0.05);双胎发情期组和双胎间情期组PI3K、Akt和PTEN基因表达量均显著高于单胎发情期组和单胎间情期组(P<0.05);单胎间情期组RPS6基因表达量显著低于其余各组(P<0.05);间情期与发情期相比,BAX基因表达显著上调(P<0.05)。Western blotting结果显示,双胎发情期ADAMST1蛋白表达量显著高于其余各组(P<0.05);双胎组与单胎组相比,PI3K和Akt蛋白均表达上调,其中,双胎发情期和双胎间情期PI3K蛋白表达量均显著高于单胎发情期组和单胎间情期组(P<0.05),双胎间情期Akt蛋白表达量最高,显著高于其余各组(P<0.05)。与对照组相比,试验组蒙古羊颗粒细胞形态异常,细胞增殖缓慢,且ADAMTS1、PI3K和Akt基因和蛋白的相对表达量均显著降低(P<0.05)。【结论】ADAMTS1、PI3K和Akt基因和蛋白在蒙古羊卵巢和颗粒细胞内的表达趋势一致,呈正向相关关系,证明ADAMTS1基因PI3K/Akt轴在蒙古羊产双胎性状中发挥重要作用。展开更多
基金supported by the earmarked fund for China Agriculture Research System (CARS-39)the International Cooperation Item of the National Natural Science Foundation of China (30410103150)+7 种基金the China Postdoctoral Science Foundation Special Funded Project (200902154)the China Postdoctoral Science Foundation (20080430470)the Key Technologies R&D Program of China during the 11th Five-Year Plan period (2006BAD13B08-07)the Natural Science Foundation of Jiangsu Province of China (BK2007556)the Basic Natural Science Foundation for Colleges and Universities, Jiangsu Province of China (NK051039)the Northern Jiangsu Technology Development Plan of China (BN2010004)the Science and Technology Program of Suzhou City of Jiangsu Province of China (SNG0911)the Jiangsu Government Scholarship for Overseas Studies Project, China
文摘In the present study we studied the genetic structure of five Chinese sheep populations of Hu sheep, Tong sheep, Small-tailed Han sheep, Tan sheep, and Wadi （WD） sheep using 15 microsatellite loci. The results showed that the FIT, FST, and FIS statistics computed for the complete dataset had the following values, 0.523±0.140, 0.363±0.131 and 0.263±0.092, respectively. All loci were significantly contributed to the genetic differentiation among population （P0.001）. There is no relationship between the scatter of pairwise FST geographical distance points as geographical distance increases between the five populations. Membership probabilities and genetic structure of sheep populations were estimated when K=2, the populations were classified into Hu, Tong, Han and WD, and Tan sheep group. However, when K=3, the populations were classified into Hu and Tong, Han and WD, and Tan sheep group. The findings supported the previous literatures that these populations are originated on different time stages from the primogenitor population and communicated genetically with each other by natural and artificial selection in different ecological environment.
