Risk Evaluation of Monochamus alternatus Hope in Jiangxi Province

[Objective]The study aimed to further strengthen the integrated management of Monochamus alternatus Hope and effectively control its spread and injury in pine forests in Jiangxi Province.[Method] By using the pest risk analysis method,the qualitative and quantitative analysis on risk of M.alternatus in Jiangxi Province was carried out.[Result] The qualitative and quantitative analysis result showed that its risk value R was 1.89,thus confirming that M.alternatus was close to high dangerous forest pest in Jiangxi Province.[Conclusion] The study provided the reference for making the policy decision for control of M.alternatus.

Space-time Distribution Pattern of Monochamus alternatus in Wanding City

[Objective] The purpose was to study space-time distribution pattern of Monochamus alternatus in Wanding City. [Method] Based on data about insect situation supervision on M. alternatus in Wanding City, using related mathematical statistics methods including linear regression analysis and spatial distribution pattern of insect population, the relationship between emergence period, emergence quantity and temperature of M. alternatus adult, spatial distribution pattern of adults and their life history were studied. [Result] M. alternatus occurred one generation per year in Wanding city, June was the maximum period of adults, which was also the key trapping period. Spatial distribution of M. alternatus was accumulative. [Conclusion] This study provided theoretical basis for further control on M. alternatus for forestry bureau of Wanding City.

Predatory behavior of Tetrigus lewisi on Monochamus alternatus

Pine wilt is a serious and fatal disease in pine forests. It is caused by the pine wood nematode, Bursaphelenchus xylophilus, transmitted by the Japanese saw beetle Monochamus alternatus. Control of this disease depends on reducing its vector population. The public is demanding environmentally friendly control methods and biological control has become increasingly seen as an impor-tant, safe and effective approach. Through our investigations both in the field and by breeding in our laboratory, a new predator of M. alternatus, Tetrigus lewisi （Coleoptera： Elateridae）, was found for the first time in China. Given our continuous observations, we are assured that the predatory behavior of the elater can be separated into four parts, i.e., foraging and sensing, killing, eating and excre-tion. They always took several hours to eat the preys that have been killed momentarily. We finally found that most of the elaters preferably attacked in the stomach and abdomen of the larva of M. alternatus.

Detection of Chaos Characteristic of Population Quantity of Adult Monochamus alternatus in Forest

[Objective] The paper was to study the chaos characteristic of population quantity sequence of adult Monochamus alternatus in forest during 2006-2010.[Method] Power spectral analysis method,correlation dimensionality and embedding dimensionality,and the maximum Lyapunov index method were adopted in the paper,respectively.[Result] The results from different chaos determination methods all showed that the population quantity sequence of adult M.alternatus among forest had chaos characteristic,belonging to chaos time sequence;when the delay time τ=5 and embedding dimensionality m=15,the correlation dimensionality in the corresponding phase space D=2.807 6,and the maximum Lyapunov index λ1=0.561 3.[Conclusion] The result provided the reference for monitoring and forecast of M.alternatus.

Influences of Post-fires on Subsequent Population of Japanese Pine Sawyer Beetles, Monochamus alternatus Hope (Coleoptera: Cerambycidae) in Masson Pine Forests

[Objective]Both fire and insect outbreaks are considered as important natural disturbance factors in many forest ecosystems,yet few studies have addressed the effects of fires on subsequent insect outbreaks.[Method]In this paper,tree mortality,larval density and vertical distribution were measured through field investigation and sampling method to evaluate the short-term response of Japanese pine sawyer beetle,Monochamus alternatus Hope to Masson pine,Pinus massoniana Lamb.in the second year after the fire in Jiangxi Province,China.[Results]compared with unburned Masson pine forest,burned Masson pine forest suffered from higher tree mortality and more pine trees were attacked by M.alternatus.Burned Masson pine tended to harbor much higher larval density further up along the trunk than unburned pine trees,and most individuals distributed in the middle section and middle-lower section of the trunk.[Significance]The results confirmed that Masson pine forest after being damaged by non-lethal fires were more susceptible to attacks by Japanese pine sawyer beetles,displaying higher population density and higher vertical distribution position.The study will provide an important guideline for the managers of Masson pine forests suffering from fires and pest invaded areas.

Effects of bark beetle pheromones on the attraction of Monochamus alternatus to pine volatiles

We evaluated the attraction ofMonochamus alternatus Hope （Coleoptera： Cerambycidae）, Dryocoetes luteus Blandford and Orthotomicus erosus Wollaston （Coleoptera： Curculionidae： Scolytinae） to multiple-funnel traps baited with the pine volatiles, ethanol and （＋）-α-pinene and the bark beetle pheromones, ipsenol and ipsdienol. M. alternatus were attracted to traps baited with ethanol and （＋）-α-pinene but not those baited with ipsdienol and ipsenol. Ipsdienol and ipsenol decreased catches ofM. alternatus in traps baited with ethanol and （＋）-α-pinene. Traps baited with either binary combinations of ethanol and （＋）-α-pinene or ipsdienol and ipsenol were attractive to D. luteus and O. erosus. The addition of ipsenol and ipsdienol to traps baited with ethanol and （＋）-α-pinene synergized attraction of O. erosus but not D. luteus .

Comparative analysis of the Monochamus alternatus immune system

The pine sawyer beetle, Monochamus alternatus, is regarded as a notorious for- est pest in Asia, vectoring an invasive pathogenic nematode, Bursaphelenchus xylophilus, which is known to cause pine wilt disease. However, little sequence information is available for this vector beetle. This hampered the research on its immune system. Based on the transcriptome of M. alternatus, we have identified and characterized 194 immunity-related genes in M. alternatus, and compared them with homologues molecules from other species known to exhibit immune responses against invading microbes. The lower number of puta- tive immunity-related genes in M. alternatus were attributed to fewer C-type lectin, serine protease （SP） and anti-microbial peptide （AMP） genes. Phylogenetic analysis revealed that M. alternatus had a unique recognition gene, galectin3, orthologues of which were not identified in Tribolium castaneum, Drosophila melanogastor, Anopheles gambiae and Apis mellifera. This suggested a lineage-specific gene evolution for coleopteran insects. Our study provides the comprehensive sequence resources of the immunity-related genes ofM. alternatus, presenting valuable information for better understanding of the molecular mechanism of innate immunity processes in M. alternatus against B. xylophilus.

Cold tolerance and cold hardening strategy of the Japanese pine sawyer Monochamus alternatus （Coleoptera： Cerambycidae）

The Japanese pine sawyer, Monochamus alternatus, is an important pine forest pest and vector transmitting the pine wilt nematode that causes pine wilt disease. Low temperatures in autumn, winter and spring often differentially affect mortality of M. alternatus larvae. In this paper, we mainly compared the differences of mortality and cold hardening of larvae from different seasons, based on supercooling point （SCP） and cumulative probability of individuals freezing （CPIF）. The cold hardening of the larvae from autumn, winter and spring seasons were largely different. Correlations between mortality and CPIF of autumn and spring larvae were highest on day 1/4, and gradually decreased with prolonged exposure duration. This beetle＇s death mainly resulted from freezing in short exposure duration. However, the correlation between mortality and CPIF of winter larvae increased gradually with the prolonged exposure duration. Death did not mainly result from freezing in long exposure duration. Autumn larvae are more susceptible and adaptable than winter and spring larvae. Winter larvae have a slight freeze-tolerance trend. Our research showed that M. alternatus came into complex cold-hardening strategies under natural selection. Freeze avoidance is the primary strategy; with prolonged exposure duration to above SCP or 〈 0℃, chill tolerance is more important; this is followed by freeze tolerance during harsh winters.

Host Preference by Monochamus alternatus (Hope) during Maturation Feeding on Pine Species and Masson Pine Provenances

Host preferences pine of the sawyer beetle, Monochamus alternates (Hope), during maturation feeding on 8 conifer trees and 40 masson pine provenances, were investigated using 3 types of laboratory bioassay of consistent feeding preference, feeding area and visitation frequency. M. alternatus adults have the highest frequency of feeding and prefer to feed on the branches of P. massoniana and P. densiflora and had significant host selectivity on 8 conifer trees in the area of Nanjing. The adult feeding vi...

Differential immune responses of Monochamus alternatus against symbiotic and entomopathogenic fungi

Monochamus alternatus, the main vector beetles of invasive pinewood nematode, has established a symbiotic relationship with a native ectotrophic fungal symbiont, Sporothrix sp. 1, in China. The immune response ofM. alternatus to S. sp. 1 in the coexistence of beetles and fungi is, however, unknown. Here, we report that immune responses ofM. alternatus pupae to infection caused by ectotrophic symbiotic fungus S. sp. 1 and entomopathogenic fungus Beauveria bassiana differ significantly. The S. sp. 1 did not kill the beetles while B. bassiana killed all upon injection. The transcriptome results showed that the numbers of differentially expressed genes in M. aIternatus infected with S. sp. 1 were 2-fold less than those infected with B. bassiana at 48 hours post infection. It was noticed that Toll and IMD pathways played a leading role in the beetle＇s immune system when infected by symbiotic fungus, but upon infection by entomopathogenic fimgus, only the Toll pathway gets triggered actively. Furthermore, the beetles could tolerate the infection of symbiotic fungi by retracing their Toll and IMD pathways at 48 h. This study provided a comprehensive sequence resource ofM. alternatus transcriptome for further study of the immune interactions between host and associated fungi.

Effects of methamidophos and deltamethrin on in vitro protein phosphorylation in Monochamus alternatus

Monochamus alternatus Hope （Coleoptera： Cerambycidae） is not only a serious pest insect to pine trees but also the main vector of pine wood nemadote Bursaphelenchus xylophilus, which causes pine wilt disease. To explore the insecticidal mechanism of insecticides to M. alternatus, we chose methamidophos and deltamethrin as the representatives of two groups of insecticides （organophosphates and pyrethroids）, which are widely used for pest control in China and investigated their effects on phosphorylation of proteins from the insect. Phosphorylation of proteins from the insect fat body and head was determined by in vitro 32P-labelling. In the fat body, deltamethrin obviously reduced basal phosphorylation levels of proteins at 111, 95, 77, and 44 kDa, but enhanced the basal phosphorylation level of a protein at 138 kDa. However, in the presence of calmodulin but not cyclic adenosine monophosphate （cAMP）, deltamethrin increased phosphorylation of the protein at 111 kDa. In the head, deltamethrin inhibited basal phosphorylation levels of proteins at 113, 98, and 51 kDa, but potentiated phosphorylation of a protein at 167 kDa activated by cAMP. Methamidophos inhibited phosphorylation of a protein at 44 kDa in the fat body. Although methamidophos did not impact basal phosphorylation levels of any proteins in the head, it inhibited calcium/calmodulin （Ca^2＋CaM）-stimulated phosphorylation of a protein at 51 kDa. Together, our data indicate that methamidophos and deltamethrin altered phosphorylation levels of various proteins in the head and fat body of the pine insect and these two kinds of insecticides acted on the proteins that can be phosphorylated in the tissues respectively, which is possibly related to their toxicity.

3种松墨天牛引诱剂诱捕效果比较

比较了3种松墨天牛引诱剂NL-01型、F2型、APF-1型对松墨天牛(Monochamus alternatus)成虫的诱捕效果。结果显示:试验组3种引诱剂诱捕松墨天牛成虫的数量相比对照组存在显著差异(P<0.05)。不同引诱剂诱捕松墨天牛成虫数量存在一定差异,总体趋势为APF-1>F2>NL-01,三者之间差异不显著。试验组3种引诱剂诱捕松墨天牛雌成虫和雄成虫的数量相比对照组存在显著差异(P<0.05),试验组之间差异不显著。试验结果表明,基于植物源和性信息素组合使用的引诱剂具有高效的诱捕效率,可以用于松墨天牛的诱杀和防治,从而降低松材线虫病(Bursaphelenchus xylophilus)的传播速度。

不同诱捕器和引诱剂组合对松褐天牛的引诱效果分析

使用松褐天牛(Monochamus alternatus Hope)诱捕器和引诱剂组成4种组合在马尾松连片林分设置10个试验区,在各试验区设置4个监测点,各监测点分别悬挂1种组合进行松褐天牛引诱比对试验,通过统计、分析使用APF-I型松褐天牛诱剂在引诱松褐天牛更具优势。