变应性气道炎症的免疫失衡受多种免疫因子调节。Th1/Th2失平衡及 Th3细胞的免疫抑制作用是这方面的研究热点。本文主要阐述干扰素-γ诱导的单核因子、单核细胞趋化蛋白-1、转化生长因子-β在变应性气道炎症中的作用机制。了解调节性 T ...变应性气道炎症的免疫失衡受多种免疫因子调节。Th1/Th2失平衡及 Th3细胞的免疫抑制作用是这方面的研究热点。本文主要阐述干扰素-γ诱导的单核因子、单核细胞趋化蛋白-1、转化生长因子-β在变应性气道炎症中的作用机制。了解调节性 T 细胞的免疫调节机制有助于开拓变应性气道炎症治疗的新方法。展开更多
Objective:To investigate the biochemical capacity,and in vitro inhibitory effects of hairy roots from two cultivars of Ficus carica L.(Sabz and Siah)on Leishmania major promastigotes and amastigotes.Methods:In the hai...Objective:To investigate the biochemical capacity,and in vitro inhibitory effects of hairy roots from two cultivars of Ficus carica L.(Sabz and Siah)on Leishmania major promastigotes and amastigotes.Methods:In the hairy roots,the activity of antioxidant enzymes compared to normal leaves and roots,and the presence of some phenolic compounds in comparison with fruits were investigated.The IC_(50) values of hairy roots in promastigotes was determined by tetrazolium-dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and trypan blue assays.By calculating the infectivity index of peripheral blood mononuclear cells(PBMCs),the leishmanicidal activity(IC_(50) values)of hairy roots for amastigotes was estimated.The effects of hairy roots(IC_(50) values)treatment on the levels of IFN-γ and iNOS expression,intracellular reactive oxygen species,and iNOS protein expression in infected-PBMCs were determined.Results:Based on antioxidant enzyme assays and high performance liquid chromatography analysis,hairy roots exhibited high antioxidant capacity and contained high levels of phenolic compounds.According to the results of tetrazolium-dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and trypan blue assays,the hairy root extracts of both cultivars showed considerable dose-dependent inhibitory effects against Leishmania major promastigotes.Depending on the concentration and exposure time,treatment of infected-PBMCs with hairy root extracts caused the generation of a significant reactive oxygen species,up-regulation of IFN-γ and iNOS genes expression,and high value of iNOS protein compared to controls.Conclusions:The findings of this study suggest that the hairy roots of Ficus carica can be considered as a promising natural source of antileishmanial agents.展开更多
目的分析慢性乙型肝炎(chronic hepatitis B,CHB)HBeAg阳性患者血清干扰素γ诱导单核细胞因子(monokine induced by IFN-γ,Mig)、白细胞介素(interle-ukin,IL)-9与乙型肝炎病毒(hepatitis B Virus,HBV)前C/BCP区变异的相关性以及其对...目的分析慢性乙型肝炎(chronic hepatitis B,CHB)HBeAg阳性患者血清干扰素γ诱导单核细胞因子(monokine induced by IFN-γ,Mig)、白细胞介素(interle-ukin,IL)-9与乙型肝炎病毒(hepatitis B Virus,HBV)前C/BCP区变异的相关性以及其对干扰素疗效影响。方法回顾性分析2015年2月至2018年2月四川省简阳市人民医院收治的110例CHB且HBeAg阳性患者资料,均给予聚乙二醇α-干扰素抗病毒治疗;治疗前扩增患者血清HBV前C/BCP区的基因片段并将患者分成HBV前C/BCP区野生型组(野生组61例)及突变型组(突变组49例),并比较两组患者血清Mig、IL-9情况以及抗病毒治疗疗效差异。结果突变组治疗前患者血清IL-9[(10.8±1.9)ng/L]明显高于野生组[(8.3±1.7)ng/L](P<0.05)。突变组治疗48周后获得完全应答率[46.9%(23/49)]显著高于野生组[16.4%(10/61)]χ^2=7.083,P=0.018;突变组治疗24周后HBsAg下降>1 lg10者较下降≤1 lg10者治疗前的血清Mig显著升高[(129.2±37.9)ng/L与(91.5±25.7)ng/L](P<0.05)。突变组治疗12、24周血清IL-9分别为(8.8±1.9)、(8.9±1.6)ng/L,均较治疗前显著降低(P均<0.05)。结论CHB且HBeAg阳性患者治疗前血清IL-9的高表达与HBV前C/BCP区的变异关系密切,IL-9的表达与干扰素抗病毒疗效无相关性;患者治疗前Mig的高表达可能对HBV前C/BCP区变异者干扰素抗病毒疗效更有利。展开更多
文摘Objective:To investigate the biochemical capacity,and in vitro inhibitory effects of hairy roots from two cultivars of Ficus carica L.(Sabz and Siah)on Leishmania major promastigotes and amastigotes.Methods:In the hairy roots,the activity of antioxidant enzymes compared to normal leaves and roots,and the presence of some phenolic compounds in comparison with fruits were investigated.The IC_(50) values of hairy roots in promastigotes was determined by tetrazolium-dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and trypan blue assays.By calculating the infectivity index of peripheral blood mononuclear cells(PBMCs),the leishmanicidal activity(IC_(50) values)of hairy roots for amastigotes was estimated.The effects of hairy roots(IC_(50) values)treatment on the levels of IFN-γ and iNOS expression,intracellular reactive oxygen species,and iNOS protein expression in infected-PBMCs were determined.Results:Based on antioxidant enzyme assays and high performance liquid chromatography analysis,hairy roots exhibited high antioxidant capacity and contained high levels of phenolic compounds.According to the results of tetrazolium-dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and trypan blue assays,the hairy root extracts of both cultivars showed considerable dose-dependent inhibitory effects against Leishmania major promastigotes.Depending on the concentration and exposure time,treatment of infected-PBMCs with hairy root extracts caused the generation of a significant reactive oxygen species,up-regulation of IFN-γ and iNOS genes expression,and high value of iNOS protein compared to controls.Conclusions:The findings of this study suggest that the hairy roots of Ficus carica can be considered as a promising natural source of antileishmanial agents.
文摘目的分析慢性乙型肝炎(chronic hepatitis B,CHB)HBeAg阳性患者血清干扰素γ诱导单核细胞因子(monokine induced by IFN-γ,Mig)、白细胞介素(interle-ukin,IL)-9与乙型肝炎病毒(hepatitis B Virus,HBV)前C/BCP区变异的相关性以及其对干扰素疗效影响。方法回顾性分析2015年2月至2018年2月四川省简阳市人民医院收治的110例CHB且HBeAg阳性患者资料,均给予聚乙二醇α-干扰素抗病毒治疗;治疗前扩增患者血清HBV前C/BCP区的基因片段并将患者分成HBV前C/BCP区野生型组(野生组61例)及突变型组(突变组49例),并比较两组患者血清Mig、IL-9情况以及抗病毒治疗疗效差异。结果突变组治疗前患者血清IL-9[(10.8±1.9)ng/L]明显高于野生组[(8.3±1.7)ng/L](P<0.05)。突变组治疗48周后获得完全应答率[46.9%(23/49)]显著高于野生组[16.4%(10/61)]χ^2=7.083,P=0.018;突变组治疗24周后HBsAg下降>1 lg10者较下降≤1 lg10者治疗前的血清Mig显著升高[(129.2±37.9)ng/L与(91.5±25.7)ng/L](P<0.05)。突变组治疗12、24周血清IL-9分别为(8.8±1.9)、(8.9±1.6)ng/L,均较治疗前显著降低(P均<0.05)。结论CHB且HBeAg阳性患者治疗前血清IL-9的高表达与HBV前C/BCP区的变异关系密切,IL-9的表达与干扰素抗病毒疗效无相关性;患者治疗前Mig的高表达可能对HBV前C/BCP区变异者干扰素抗病毒疗效更有利。
