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Analysis of the monosaccharide composition of fucoidan by precolumn derivation HPLC 被引量:6
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作者 张晶晶 张全斌 +2 位作者 王晶 石学连 张忠山 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2009年第3期578-582,共5页
We developed an HPLC method for analysis of the monosaccharide composition of fucoidans. The fucoidan was hydrolyzed into monosaccharides with 2 mol/L trifluoroacetic acid. Using ribose as the internal standard, the m... We developed an HPLC method for analysis of the monosaccharide composition of fucoidans. The fucoidan was hydrolyzed into monosaccharides with 2 mol/L trifluoroacetic acid. Using ribose as the internal standard, the monosaecharide derivatives, obtained with 1-Phenyl-3-methyl-5- pyrazolone (PMP), were separated by reverse-phase HPLC using a gradient elution process, and monitored by ultraviolet detection at 245 nm. In the concentration range of 0.1-2.0 mmol/L, the peak area of each monosaccharide had a good linear relationship with its concentration (r^2〉0.998). The average recoveries of mannose, rhamnose, glucuronic acid, glucose, galactose, xylose, and fucose were 86.2%, 95.1%, 62.5%, 102.0%, 94.8%, 66.6%, and 105.1%, respectively. This method was accurate and had good reproducibility and could be used to determine the monosaccharide contents of fucoidans. 展开更多
关键词 FUCOIDAN monosaccharide DERIVATION HPLC
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A new monosaccharide from Swertia punicea Hemsl 被引量:1
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作者 Luan Yuan Tian Jia Chun Chen Jin Bo Fang Qun Zhou Xue Bai Jian Qiu Zhou Xiang Hong Chen 《Chinese Chemical Letters》 SCIE CAS CSCD 2009年第6期684-686,共3页
A new compound named 1-hydrate, 3-deoxy-α-D-tagatofuranose was isolated from Swertia punicea Hemsl. The structure of compound was determined by 1D and 2D NMR, HRESIMS techniques.
关键词 Swertia punicea Hemsl. monosaccharide 1-Hydrate 3-Deoxy -α-D-tagatofuranose
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Monosaccharide Recovery from Peat and Peatified Wood by Ultrasonication Pretreatment and Hydrothermal Treatment 被引量:1
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作者 Moriyasu Nonaka Tsuyoshi Hirajima Satoshi Kumagai 《International Journal of Clean Coal and Energy》 2017年第2期31-39,共9页
Peat and peatified wood are significant carbohydrate resources in tropical rainforests. The carbohydrates of cellulose and hemicellulose are important sources of monosaccharides for both vital activities and industria... Peat and peatified wood are significant carbohydrate resources in tropical rainforests. The carbohydrates of cellulose and hemicellulose are important sources of monosaccharides for both vital activities and industrial applications, such as furan production of furfural and hydroxymethyl furfural. Hydrothermal treatment at 200°C and ultrasonic irradiation pretreatment were used to recover monosaccharides from the abovementioned resources. The monosaccharide recovery from peat was shown to be higher than that from peatified wood. The conversion to organic acids is considered to proceed rapidly because acids are always detected with monosaccharides. This conversion is outstripped by the organic acid-to-gas reaction for treatment times longer than 20 min. The monosaccharide recovery from peatified wood was improved by the ultrasonication pretreatment. It is considered that ultrasonic irradiation broke down lignin blockages, enabling water molecules to access the carbohydrates more easily in the subsequent hydrothermal treatment. 展开更多
关键词 PEAT Peatified WOOD HYDROTHERMAL Treatment Ultrasonication monosaccharide
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SYNTHESIS,HELICITY,AND BIOLOGICAL PROPERTIES OF MONOSACCHARIDE-CONTAININGPOLY(PHENYLACETYLENE)S
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作者 Jacky W. Y. Lam Kevin K. L. Cheuk +2 位作者 Bing-shi Li Yong Xie Ben-zhong Tang 《Chinese Journal of Polymer Science》 SCIE CAS CSCD 2007年第2期119-122,共4页
Poly(phenylacetylene)s beating monosaccharide pendant groups are synthesized in high yields by [Rh(nbd)Cl]2 catalyst. The polymers have high molecular weights and give satisfactory spectroscopic data corresponding... Poly(phenylacetylene)s beating monosaccharide pendant groups are synthesized in high yields by [Rh(nbd)Cl]2 catalyst. The polymers have high molecular weights and give satisfactory spectroscopic data corresponding to their molecular structures. They are thermally quite stable (≥ 300℃) and show strong circular dichroism signals in the visible spectral region owing to the helicity of the polyene backbone. The monosaccharide-containing polyacetylenes are cytophilic and can stimulate the growth of living cells. 展开更多
关键词 BIOCOMPATIBILITY BIOMIMETIC HELICITY monosaccharideS POLYACETYLENES Sugars.
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A new lipophilic monosaccharide from Erigeron annuus
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作者 Sheng Chao Yang Jian Xin Pu +3 位作者 Ying Lu Feng Hui Xiao Wei Lie Xiao Han Dong Sun 《Chinese Chemical Letters》 SCIE CAS CSCD 2008年第10期1231-1233,共3页
A new lipophilic monosaccharide, erigearide A (1), was isolated from the aerial parts of Erigeron annuus (Lima.) Pers. Its structure was elucidated by analysis of spectroscopic evidence.
关键词 Erigeron annuus Lipophilic monosaccharide Erigearide A
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Low fermentable oligosaccharides, disaccharides,monosaccharides, and polyols diet in children
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作者 Ioana Fodor Sorin Claudiu Man Dan L Dumitrascu 《World Journal of Clinical Cases》 SCIE 2019年第18期2666-2674,共9页
Irritable bowel syndrome (IBS) is a lifelong condition with a high prevalence among children and adults. As the diet is a frequent factor that triggers the symptoms, it has been assumed that by avoiding the consumptio... Irritable bowel syndrome (IBS) is a lifelong condition with a high prevalence among children and adults. As the diet is a frequent factor that triggers the symptoms, it has been assumed that by avoiding the consumption of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAP), the symptoms might be improved. Therefore, in the past decade, low FODMAP diet has been intensively investigated in the management of IBS. The capacity of FODMAPs to trigger the symptoms in patients with IBS was related to the stimulation of mechanoreceptors in the small and large intestine. This stimulation appears as a response to a combination of increased luminal water (the osmotic effect) and the release of gases (carbon dioxide and hydrogen) due to the fermentation of oligosaccharides and malabsorption of fructose, lactose and polyols. Numerous studies have been published regarding the efficacy of a low FODMAP diet compared to a traditional diet in releasing the IBS symptoms in adults, but there are only a few studies in the juvenile population. The aim of this review is to analyze the current data on both low FODMAP diet in children with IBS and the effects on their nutritional status and physiological development, given the fact that it is a restrictive diet. 展开更多
关键词 Low fermentable OLIGOSACCHARIDES DISACCHARIDES monosaccharideS POLYOLS DIET CHILDREN Irritable bowel syndrome Symptom
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Rapid Method for the Determination of Total Monosaccharide in <i>Enterobacter cloacae</i>Strains Using Fourier Transform Infrared Spectroscopy
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作者 Richard J. Delle Bovi Allan Smits Harry M. Pylypiw 《American Journal of Analytical Chemistry》 2011年第2期212-216,共5页
Fourier Transform Infrared Spectroscopy (FTIR) was used to quantify total monosaccharide content in the bacterium Enterobacter cloacae and several of its biofilm mutants. Bacterial biofilm samples were grown on trypti... Fourier Transform Infrared Spectroscopy (FTIR) was used to quantify total monosaccharide content in the bacterium Enterobacter cloacae and several of its biofilm mutants. Bacterial biofilm samples were grown on trypticase soy agar, and 30 μL aliquots of aqueous sample bacterial plus biofilm were deposited into the center of barium fluoride crystals and dried at 50°C for 1-hour before being scanned by FTIR. The total amounts of monosaccharides were estimated using the absorbance of the mono-saccharide peak, 1192 - 958 cm–1, and normalized using the amide II peak, 1585 - 1483 cm–1. This method provided a linear correlation between the absorbance of the monosaccharide peak and concentration of monosaccharide in standard monosaccharides, fructose, glucose, mannose, and rhamnose, over a concentration range of 0.5 - 2.0 mg/mL. 展开更多
关键词 Enterobacter CLOACAE Biofilm Glucose Fructose MANNOSE RHAMNOSE monosaccharideS Fourier Transform Infrared Spectroscopy FTIR
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Formation of Oligosaccharide from Monosaccharide by Pentacoordinate Oxyphosphorane
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作者 Dong Yan QIN Yong JU +2 位作者 Yu GUO Yu Fen ZHAO Qin Zhu WANG(Bioorganic Phosphorus Chemistry Labothory, Department of Chemistry, Tsinghua University, Beijing 100084) 《Chinese Chemical Letters》 SCIE CAS CSCD 1997年第4期303-304,共2页
Oxyphosphorane reacted with glucose to give disaccharide which was confirmed by FAB-MS and C-13-NMR. The mechanism for formation of oligosaccharides through the participation of phosphorus was proposed.
关键词 Formation of Oligosaccharide from monosaccharide by Pentacoordinate Oxyphosphorane
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Efficient oxidation of monosaccharides to sugar acids under neutral condition in flow reactors with gold-supported activated carbon catalysts
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作者 Ziqin Gong Zengyong Li +3 位作者 Xu Zeng Fengxia Yue Wu Lan Chuanfu Liu 《Frontiers of Chemical Science and Engineering》 SCIE EI CSCD 2024年第9期113-123,共11页
A significant reaction in the synthesis of biomass-based chemicals is the catalyst-based and targeted oxidation of monosaccharides into valuable sugar acids.In this study,an activated carbon supported gold catalyst wa... A significant reaction in the synthesis of biomass-based chemicals is the catalyst-based and targeted oxidation of monosaccharides into valuable sugar acids.In this study,an activated carbon supported gold catalyst was used to oxidize glucose and xylose to gluconic acid and xylonic acid under neutral condition.Optimization of reaction conditions for the catalysts was performed using both a batch reactor and a flow-through reactor.In a batch reactor,the yields of gluconic and xylonic acid reached 93%and 92%,respectively,at 90℃ within 180 min.In a flow reactor,both reactions reached a similar yield at 80℃ with the weight hourly space velocity of 47.1 h^(-1).The reaction kinetics were explored in the flow reactor.The oxidation of glucose and xylose to gluconic and xylonic acid followed a first-order kinetics and the turnover frequency was 0.195 and 0.161 s^(-1),respectively.The activation energy was evaluated to be 60.58 and 59.30 kJ·mol^(-1),respectively.This study presents an environmentally friendly and feasible method for the selective oxidation of monosaccharides using an activated carbon supported gold catalyst,benefiting the high-value application of carbohydrates. 展开更多
关键词 monosaccharides oxidation gluconic acid xylonic acid flow reactor KINETICS
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Diverse Functional Roles of Monosaccharide Transporters and their Homologs in Vascular Plants: A Physiological Perspective 被引量:34
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作者 Thomas L. Slewinski 《Molecular Plant》 SCIE CAS CSCD 2011年第4期641-662,共22页
Vascular plants contain two gene families that encode monosaccharide transporter proteins. The classical monosaccharide transporter(-like) gene superfamily is large and functionally diverse, while the recently ident... Vascular plants contain two gene families that encode monosaccharide transporter proteins. The classical monosaccharide transporter(-like) gene superfamily is large and functionally diverse, while the recently identified SWEET transporter family is smaller and, thus far, only found to transport glucose. These transporters play essential roles at many levels, ranging from organelles to the whole plant. Many family members are essential for cellular homeostasis and reproductive success. Although most transporters do not directly participate in long-distance transport, their indirect roles greatly impact carbon allocation and transport flux to the heterotrophic tissues of the plant. Functional characterization of some members from both gene families has revealed their diverse roles in carbohydrate partitioning, phloem function, resource allocation, plant defense, and sugar signaling. This review highlights the broad impacts and implications of monosaccharide transport by describing some of the functional roles of the monosaccharide transporter(-like) superfamily and the SWEET transporter family. 展开更多
关键词 monosaccharide transporters sugar transport membrane transport HEXOSES sugar alcohols carbohydrate partitioning.
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Pyrolysis Mechanism of Hemicellulose Monosaccharides in Different Catalytic Processes 被引量:1
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作者 WANG Shurong RU Bin LIN Haizhou SUN Wuxing YU Chunjiang LUO Zhongyang 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2014年第5期848-854,共7页
The pyrolysis behaviors of four different hemicellulose monosaccharides, namely, two pentoses(xylose and arabinose) and two hexoses(mannose and galactose) catalyzed by HZSM-5 were investigated. The effects of two ... The pyrolysis behaviors of four different hemicellulose monosaccharides, namely, two pentoses(xylose and arabinose) and two hexoses(mannose and galactose) catalyzed by HZSM-5 were investigated. The effects of two different processes by which the catalyst comes into contact with the substrate, namely, mixed with monosaccha- ride(in-bed) or layered above monosaccharide(in situ), were compared. Evolution characteristics of typical pyrolytic products(H20, CO2, acids, furans and aromatics) were achieved by thermogravimetry-Fourier transform infrared spectroscopy. The in-bed catalytic process significantly lowered the pyrolytic temperature and increased the produc- tion of furans and acids at a low temperature by enhancing dehydration, retro-aldol fragmentation and Grob fragmen- tation. During the in situ catalytic process, volatiles generated from monosaccharides passed through a catalyst bed and underwent further dehydration, decarboxylation, and decarbonylation, significantly lowering the yields of acids and furans. The yield of aromatics was enhanced, and the corresponding volatilization temperature was lowered, es- pecially under the in-bed catalytic conditions. Pentoses entered into the zeolite pores more easily than hexoses did because of their smaller molecular size; thus, the in-bed catalytic process drastically affected pentose pyrolysis. 展开更多
关键词 HEMICELLULOSE monosaccharide TG-FTIR HZSM-5 PYROLYSIS
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The effect of monosaccharides on self-assembly of benzenetricarboxamides
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作者 Jue Wang Wenjing Qi Guosong Chen 《Chinese Chemical Letters》 SCIE CAS CSCD 2019年第3期587-591,共5页
The interaction between monosaccharides exhibits an important role in the assembly of monosaccharide-containing molecules. In this work, three common monosaccharides, glucose, galactose and mannose, are employed to in... The interaction between monosaccharides exhibits an important role in the assembly of monosaccharide-containing molecules. In this work, three common monosaccharides, glucose, galactose and mannose, are employed to investigate the effect of monosaccharide on the self-assembly of benzenetricarboxamide(BTA) core-containing molecules. In the presence of monosaccharides, three benzenetricarboxamide derivatives aggregate into different ordered structures. When alanine linkers are introduced to these molecules between the core and the monosacchride, morphologies of three types of monosaccharide BTAs turned to disordered, meanwhile their structures become similar with the increase of the length of alanine linkers, indicating the disappearance of the monosaccharide effects. 展开更多
关键词 monosaccharide SELF-ASSEMBLY Benzenetricarboxamide DYNAMIC LIGHT scatting (DLS) Depolarized DYNAMIC LIGHT scatting (DDLS)
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Enthalpy and entropy interaction parameters of sodium chloride with some monosaccharides in water
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作者 ZHUO Kelei, WANG Jianji, BAI Guangyue, YAN Haike & WANG HangingSchool of Chemistry and Environmental Science, Henan Normal University, Xinxiang 453002, China Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China 《Science China Chemistry》 SCIE EI CAS 2004年第1期59-66,共8页
Dilution enthalpies of sodium chloride and some monosaccharides (glucose, galactose, xylose, arabinose, and fructose) in water and mixing enthalpies of aqueous sodium chloride and these monosaccharide solutions were m... Dilution enthalpies of sodium chloride and some monosaccharides (glucose, galactose, xylose, arabinose, and fructose) in water and mixing enthalpies of aqueous sodium chloride and these monosaccharide solutions were measured by using an improved precision semimicro-titration calorimeter. Transfer enthalpies of sodium chloride from water to aqueous saccharide solutions were evaluated as well as enthalpy interaction parameters of sodium chloride with these monosaccharides in water. Combined with Gibbs energy interaction parameters, entropy interaction parameters were also obtained. The results show that interactions of the saccharides with sodium chloride depend on the stereochemistry of saccharide molecules. These interaction parameters can identify stereochemical structure of saccharide molecules. Keywords sodium chloride - monosaccharide - enthalpy interaction parameter - entropy interaction parameter - calorimetry 展开更多
关键词 sodium chloride monosaccharide ENTHALPY INTERACTION parameter ENTROPY INTERACTION parameter calorimetry.
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A new strategy for high-throughput identification of human gut microbiota containing specific monosaccharide
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作者 Qizheng Wu Fang Liu +8 位作者 Yu Song Qingyun Meng Xunlian Zhang Huizi Li Jingyu Sun Hongqi Gao Peng George Wang Yun Kong Min Chen 《Science China(Life Sciences)》 SCIE CAS CSCD 2019年第7期985-987,共3页
Dear Editor,The human gastrointestinal tract is colonized by trillions of microorganisms,the majority of which cannot be cultured (Wu et al.,2018).Numerous glycans are expressed on the surface of gut microbiota that a... Dear Editor,The human gastrointestinal tract is colonized by trillions of microorganisms,the majority of which cannot be cultured (Wu et al.,2018).Numerous glycans are expressed on the surface of gut microbiota that are characteristic of different strains,and the diversity of these glycans is partially attributed to variations in their monosaccharides. 展开更多
关键词 EDITOR GLYCANS monosaccharideS
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Methods for determination of absolute configuration of monosaccharides
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作者 nan zhang wei-xing huang +4 位作者 gui-yang xia mahmood brobbey oppong li-qing ding pan li feng qiu 《Chinese Herbal Medicines》 CAS 2018年第1期14-22,共9页
Monosaccharides are one of the most important structural components of biomolecules, such as polysac- charides, nucleic acids, glycolipids and glycoproteins. In structural analysis of polysaccharides and gly- coconjug... Monosaccharides are one of the most important structural components of biomolecules, such as polysac- charides, nucleic acids, glycolipids and glycoproteins. In structural analysis of polysaccharides and gly- coconjugates, the absolute configurations (D or L) of the constituent monosaccharides are usually deter- mined by measurement of the optical rotation, CD spectra or characteristic chromatographic retention behavior. However, each method has its unique advantages and limitations which should be considered while using them. In this review, an overview of the different methods for the determination of absolute configuration of monosaccharides and their underlying principles are summarized to serve as a reference for researchers. 展开更多
关键词 absolute configuration CD chemical derivatization - chromatography monosaccharideS optical rotation
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MONOSACCHARIDE COMPOSITION OF POLYSACCHARIDES THROUGH HILIC-UPLC-TQ-MS/MS
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作者 Yong-Gang Xia Li-Ming Sun +2 位作者 Jun Liang Qiu-Hong Wang Hai-Xue Kuang 《World Journal of Traditional Chinese Medicine》 2015年第4期92-92,共1页
A simple,sensitive and direct method was developed for compositional analysis of plant polysaccharides using hydrophilic interaction ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass s... A simple,sensitive and direct method was developed for compositional analysis of plant polysaccharides using hydrophilic interaction ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry(HILIC-UPLC-TQ-MS/MS)in multiple-reaction monitoring(MRM).The effects of chromatographic columns,scan time,additive types, 展开更多
关键词 HILIC monosaccharide COMPOSITION OF POLYSACCHARIDES THROUGH HILIC-UPLC-TQ-MS/MS
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Analysis of the linkage positions and isomers of monosaccharide units in oligosaccharides by negative secondary ion mass spectrometry in combination with the stereoselective reaction with substituted boronic acid
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作者 YAN,Lin FANG,Yi-Wei Institute of Chemistry,Chinese Academy of Sciences,Beijing 100080 《Chinese Journal of Chemistry》 SCIE CAS CSCD 1993年第1期53-58,共8页
The negative secondary ion mass spectrometry,in combination with the stereoselective derivatizations with substituted boronic acid RB(OH)_2,was used in the analysis of fourteen oligosac- charides.The mass spectra of t... The negative secondary ion mass spectrometry,in combination with the stereoselective derivatizations with substituted boronic acid RB(OH)_2,was used in the analysis of fourteen oligosac- charides.The mass spectra of the derivatives provide information on their linkage Positions and iso- merism of the individual monoscaccharide units.The results indicated that among the derivatives of the oligosaccharides analyzed,those with 1—4 and 1—6 linkages all presented the ion peaks at m/z 287,sometimes one more peak at m/z 449.Furthermore,a relationship was found between the linkage positions and the intensity orders of the derivative ions.Finally,the derivatives of the disaccharides with a galactose presented an intense ion peak at m/z 347,and those of oligosaccharides with 1—6 linkage to a galactose at terminal presented the ion at m/z 317.In the case of oligosaccharides with a fructose residue,characteristic ion of m/z 155 was produced.The conditions of stereoselective derivatizations and mass spectrometry were studied,in order to obtain a better reproducibility of the mass spectra. 展开更多
关键词 Analysis of the linkage positions and isomers of monosaccharide units in oligosaccharides by negative secondary ion mass spectrometry in combination with the stereoselective reaction with substituted boronic acid acid
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Characteristics of dissolved sugars in the Southern Yap Trench from sea surface to hadal zone
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作者 Dong CUI Chengjun SUN +3 位作者 Chaonan GUO Wei CAO Fenghua JIANG Haibing DING 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2023年第6期2117-2133,共17页
2,4,6-Tripyridine-s-triazine(TPTZ)spectrophotometric method was applied to determine the concentrations of dissolved monosaccharides(MCHO),polysaccharides(PCHO),and total carbohydrate(TCHO)in seawater samples collecte... 2,4,6-Tripyridine-s-triazine(TPTZ)spectrophotometric method was applied to determine the concentrations of dissolved monosaccharides(MCHO),polysaccharides(PCHO),and total carbohydrate(TCHO)in seawater samples collected from sea surface to hadal zone and sediment-seawater interface of the Southern Yap Trench in the Western Pacific Ocean.Results show that the concentrations of MCHO,PCHO,and TCHO ranged from 6.3 to 22.3μmol C/L,1.1 to 25.4μmol C/L,and 12.1 to 44.9μmol C/L,respectively,from the euphotic layer to the hadal zone of the trench.At different sampling stations,the concentrations of MCHO,PCHO,and TCHO in the seawater showed complex vertical variation characteristics,but the overall variation trends were decreasing with water depth.In the Southern Yap Trench,the maximum concentration of MCHO in the seawater appeared in the euphotic layer,and the minimum in the hadal zone.The maximum concentration of PCHO appeared in the euphotic layer,and the minimum in the bathypelagic layer.The water layer where the maxima and minima of the average concentration of TCHO appeared was consistent with that of PCHO.PCHO was the major component of TCHO in the seawater of the Southern Yap Trench.In the seawater from the sediment-seawater interface,the concentrations of MCHO,PCHO,and TCHO ranged from 8.4 to 10.6μmol C/L,3.8 to 5.8μmol C/L,and 12.2 to 15.2μmol C/L,respectively,and MCHO was the major component of TCHO.The key factors affecting the concentration and existing forms of dissolved sugars in the seawater of the Southern Yap Trench included photosynthesis,respiration,polysaccharide hydrolysis,adsorption and desorption of particulate matter,trench“funnel effect”,deep ocean currents,sediment resuspension,and etc.This study provided fundamental data about labile organic matter in abyss and hadal zone of marine environment,which is significant for further understanding of deep-sea organic carbon cycle. 展开更多
关键词 deep sea Yap Trench ABYSS hadal zone monosaccharideS POLYSACCHARIDES
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The Effect of Thoracic Operation on Glucose Transporter-4 mRNA Expression by Preoperative Infusion of Glucose
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作者 陈新春 钱燕宁 +1 位作者 傅诚章 林桂芳 《Journal of Nanjing Medical University》 2004年第1期7-10,共4页
Objective: To investigate the changes in glucose transporter-4(Glut-4) mRNA expression in skeletal muscle before and after the thoracic operation and to observe the changes in Glut-4 mRNA expression by preoperative in... Objective: To investigate the changes in glucose transporter-4(Glut-4) mRNA expression in skeletal muscle before and after the thoracic operation and to observe the changes in Glut-4 mRNA expression by preoperative infusion of glucose. Methods: Twelve cases of elective thoracic operation were randomly divided into two groups, namely ordinary group Ⅰ and glucose infusion group Ⅱ. One gram of intercostal muscle was taken while thorax being opened and closed from patients under general anesthesia. Total RNA of the muscle cells was extracted by TRIzol one-step assay. Reverse transcription-competitive polymerase chain reaction (RT-PCR) was used to determine the Glut-4 mRNA amplification products with β-actin mRNA as an internal control. The Glut-4 mRNA expression was expressed by targeted gene /β-actin ×100%. The plasma glucose and insulin levels were determined at the same time.Results: Glut-4 mRNA expression was significantly reduced(P<0.05) and plasma glucose level increased (P<0.05), while thorax was being closed as compared with those while being opened. However, Glut-4 mRNA expression in glucose infusion group Ⅱ was significantly higher than ordinary group Ⅰ (P<0.01) and plasma glucose level in group Ⅱ was lower than group Ⅰ(P<0.05) when thorax was being closed. Conclusion: The results indicate that the synthesis of Glut-4 is suppressed by the surgical stress of thoracic operation under general anesthesia. We found that preoperative infusion glucose can increase Glut-4 mRNA expression at the same surgical stress and relieve postoperative insulin resistance. 展开更多
关键词 GLUCOSE insulin resistance monosaccharide transporter proteins thoracic surgical procedures
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Diet and functional dyspepsia: Clinical correlates and therapeutic perspectives 被引量:17
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作者 Marcella Pesce Martina Cargiolli +5 位作者 Sara Cassarano Barbara Polese Barbara De Conno Laura Aurino Nicola Mancino Giovanni Sarnelli 《World Journal of Gastroenterology》 SCIE CAS 2020年第5期456-465,共10页
Hypervigilance and symptoms anticipation,visceral hypersensitivity and gastroduodenal sensorimotor abnormalities account for the varied clinical presentation of functional dyspepsia(FD)patients.Many patients recognize... Hypervigilance and symptoms anticipation,visceral hypersensitivity and gastroduodenal sensorimotor abnormalities account for the varied clinical presentation of functional dyspepsia(FD)patients.Many patients recognize meals as the main triggering factor;thus,dietary manipulations often represent the first-line management strategy in this cohort of patients.Nonetheless,scarce quality evidence has been produced regarding the relationship between specific foods and/or macronutrients and the onset of FD symptoms,resulting in nonstandardized nutritional approaches.Most dietary advises are indeed empirical and often lead to exclusion diets,reinforcing in patients the perception of“being intolerant”to food and self-perpetuating some of the very mechanisms underlying dyspepsia physiopathology(i.e.,hypervigilance and symptom anticipation).Clinicians are often uncertain regarding the contribution of specific foods to dyspepsia physiopathology and dedicated professionals(i.e.,dietitians)are only available in tertiary referral settings.This in turn,can result in nutritionally unbalanced diets and could even encourage restrictive eating behaviors in severe dyspepsia.In this review,we aim at evaluating the relationship between dietary habits,macronutrients and specific foods in determining FD symptoms.We will provide an overview of the evidence-based nutritional approach that should be pursued in these patients,providing clinicians with a valuable tool in standardizing nutritional advises and discouraging patients from engaging into indiscriminate food exclusions. 展开更多
关键词 Functional dyspepsia Dietary habits Food intolerances Fermentable oligosaccharides disaccharides monosaccharides and polyols Gluten-sensitivity DIET
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