Aim To find the original active compounds from three medicinal plants of Menispermaceae family in Qinling Montain,Shaanxi,China.Method Several kinds of isolation techniques such as liquid-liquid partition,column chrom...Aim To find the original active compounds from three medicinal plants of Menispermaceae family in Qinling Montain,Shaanxi,China.Method Several kinds of isolation techniques such as liquid-liquid partition,column chromatography,thin layer chromatography and sephadex LH-20 gel chromatography are used to separate the compounds and all kinds of identification techniques such as mass,high resolution mass,1H-NMR,13C-NMR,all kinds of 2D-NMR,CD spectra and X-ray diffraction analysis are used to determine the structure of the isolated compounds;several enzymes linked immunosorbent assay are used to test the biological activities of some isolated compounds.Results 30 alkaloids were isolated and identified from Sinomenium acutum of which 8 are new compounds;nine alkaloids were isolated and identified from Menispermum dauricum of which 6 are new compounds;six alkaloids were isolated and identified from Stephania epigaea.Among all the identified alkaloids,six compounds show protective effects against hydrogen peroxide induced cell injury;eight compounds show weak or strong cytotoxicity activity;one compound shows neuroprotective effect against hydrogen peroxide and.β-amyloid25-35-induced oxidative injury.Conclusion These three plants of Menispermaceae family may be considered as the potential drugs for Alzheimer's disease therapy and anticancer agents.展开更多
文摘Aim To find the original active compounds from three medicinal plants of Menispermaceae family in Qinling Montain,Shaanxi,China.Method Several kinds of isolation techniques such as liquid-liquid partition,column chromatography,thin layer chromatography and sephadex LH-20 gel chromatography are used to separate the compounds and all kinds of identification techniques such as mass,high resolution mass,1H-NMR,13C-NMR,all kinds of 2D-NMR,CD spectra and X-ray diffraction analysis are used to determine the structure of the isolated compounds;several enzymes linked immunosorbent assay are used to test the biological activities of some isolated compounds.Results 30 alkaloids were isolated and identified from Sinomenium acutum of which 8 are new compounds;nine alkaloids were isolated and identified from Menispermum dauricum of which 6 are new compounds;six alkaloids were isolated and identified from Stephania epigaea.Among all the identified alkaloids,six compounds show protective effects against hydrogen peroxide induced cell injury;eight compounds show weak or strong cytotoxicity activity;one compound shows neuroprotective effect against hydrogen peroxide and.β-amyloid25-35-induced oxidative injury.Conclusion These three plants of Menispermaceae family may be considered as the potential drugs for Alzheimer's disease therapy and anticancer agents.
