诺丽(Morinda citrifolia L.)离体快速繁殖研究

以诺丽（Morinda citrifolia L．）种子为试材．诺丽子叶和下胚轴均能单独由细胞分裂素BA0．7～2．0mg／L谤导不定芽发生，不定芽可直接从外植体发生，也可从愈伤组织发生，添加生长素NAA0．05～0．1mg／L则完全抑制不定芽发生，同时强烈促进愈伤组织和不定根发生．茎段在BA0．5～5．0mg／L或ZT 1．5mg／L或KN 1．5mg／L时均能通过腋芽萌发和不定芽发生而增殖．芽梢在NAA 0．1mg／L、IBA 0．1mg／L或IAA 0．1mg／L均有根群发生，但NAA 0．1mg／L诱导生根时切口愈伤组织较多，部分不定根由愈伤组织发生，而IAA 0．1mg／L诱导生根时根群欠发达，以IBA 0．1mg／L为最佳．试管苗移植到泥炭上10d后即可在全光照下生长．

诺丽(Morinda citrifolia Linn.)离体培养研究

为诺丽遗传转化奠定基础及诺丽工厂化育苗提供依据,本实验以诺丽茎尖及带芽茎段为外植体,以3%MS培养基添加不同的激素组合进行离体培养,选择最佳的培养组合。实验结果表明,茎尖在MS或添加NAA(0.3 mg/L)的MS培养基中均可得到完整植株,且在后者中的长势更好。该研究成功建立了诺丽离体再生体系,为规模化生产及遗传转化提供了技术支撑。

Extraction and Characterization of Antioxidant Compositions From Fermented Fruit Juice of Morinda citrifolia (Noni)

Extraction and characterization of antioxidative compositions from the extracts of fermented Xisha Noni （Morinda citrifolia L.） juice were studied. The antioxidative constituents of 184.6 g freeze-dried extracts of naturally fermented Xisha Noni juice were isolated successfully by petroleum ether, EtOAc and n-BuOH solvents, and the antioxidative effects were measured according to scavenging activity against hydroxyl generated in Fenton reaction system and superoxide anion radicals in pyrogallol autoxidation system. The EtOAc extract exhibited most significantly higher （P 〈 0.01） antioxidative activity than mannitol or vitamin C, while the petroleum ether and n-BuOH extracts showed lower activities compared to mannitol. Three antioxidant phenolic compounds, isoscopoletin, aesculetin and 3,3＇,4＇,5,7-pentahydroxyflavone （quercetin） were isolated from the EtOAc extract by several chromatography techniques for the first time. The results suggest that several compounds, in particular, the phenolic compounds, contribute separately or synergistically to the antioxidative activity of fermented Noni fruit juice.

Analysis of Volatile Components in Fresh Fruit of Morinda citrifolia L.from Different Origins Based on HS-SPME-GC/MS

[Objectives]This study was conducted to clarify the differences of volatile components in fresh fruit of Morinda citrifolia L.from different origins.[Methods]The method of HS-SPME-GC/MS detection was used to determine the volatile chemical components in fresh fruit of M.citrifolia.[Results]52,52 and 45 volatile components were identified from the fresh fruit of M.citrifolia from Xisha,Wanning and Haikou,respectively.Among them,the number and content of the identified esters were relatively high,mainly methyl caprylate,ethyl caprylate,4-pentenyl caproate,3-methylbuten-2-enyl caprylate,methyl caproate,and ethyl caproate.There were also fatty acids,alcohols,phenols,ketones,aldehydes and other substances.There were 33 common ingredients in the fruit from the three origins,mainly including caprylic acid,caproic acid,capric acid,methyl caprylate,ethyl caprylate,4-pentenyl caproate,3-methylbutene-2-enyl caprylate,methyl caproate,ethyl caproate,methyl caprate and hexyl caproate.[Conclusions]The types and contents of volatile components in fresh fruit of M.citrifolia from different origins were significantly different.

海巴戟(Morinda citrifolia L.)组织培养研究

以海巴戟（Morinda citrifolia L．）种子为试材，在不剥除种皮的情况下，在MS无激素培养基上播种1年内未见发芽，在剥除种皮的情况下，在MS无激素培养基上发芽率最高，50d内可达75％。海巴戟子叶和下胚轴均能单独由细胞分裂素BA0．7～2．0mg／L诱导不定芽发生，不定芽可直接从外植体发生，也可从愈伤组织发生，添加生长素NAA0．05～0．1mg／L则完全抑制不定芽发生，同时强烈促进愈伤组织生长和不定根发生。带芽茎段在BA1．5mg／L配合低浓度生长素时均能通过腋芽萌发和不定芽发生而增殖。芽梢在NAA0．1mg／L、IBA0．1mg／L或IAA0．1mg／L均有根群发生，但NAA0．1mg／L诱导生根时切口愈伤组织较多，部分不定根由愈伤组织发生。而IAA0．1mg／L诱导生根时根群欠发达，以IBA0．1mg／L最佳。

Study on the Extraction Process of Total Flavonoids from Morinda citrifolia L.

[Objectives]This study was conducted to explore the best extraction technology of total flavonoids from Morinda citrifolia L.[Methods]An orthogonal test was carried out to select the best extraction process with the yield of total flavonoids in the extract as the investigation index.[Results]Among the four factors of experimental design,the extraction time had a very significant impact on the results,the ethanol concentration had a significant impact on the results,and the extraction temperature and ethanol dosage had no significant impacts on the results.The optimal extraction process conditions for the total flavonoids of M.citrifolia were as follows:extraction time 1.5 h,ethanol concentration 90%,extraction temperature 60-65℃,and ethanol dosage 1∶20(g/ml).The total flavonoids in M.citrifolia fruit were extracted according to the optimal extraction process conditions,and the yield was 2.936%.[Conclusions]This study provides a theoretical basis for obtaining the optimal extraction process conditions for total flavonoids of M.citrifolia.

<i>Morinda citrifolia</i>(Noni) Fruit Juice Inhibits SARS-CoV-2 Spike Protein Binding of Angiotensin-Converting Enzyme 2 (ACE2)

COVID-19 is a global pandemic that has claimed millions of lives. This disease is caused by a coronavirus, SARS-CoV-2, which requires the binding of its spike protein to angiotensin-converting enzyme 2 (ACE2) for infection of the host cell. Morinda citrifolia (noni) fruit juice has antiviral activity that involves enhancement of immune system function. SARS-CoV-2 spike-ACE2 interaction experiments were carried out to further investigate the antiviral properties of noni juice and its major iridoids. Noni juice inhibited binding by approximately 69%. Scandoside was the most active of the three iridoids evaluated, reducing average spike protein-ACE2 interaction by 79.25%. The iridoids worked synergistically towards inhibiting spike protein binding when assayed together, improving activity by more than 22% above the expected level. But the modest activity of the most abundant iridoid, deacetylasperulosidic acid, indicates that other phytochemicals (i.e. scopoletin, quercetin, rutin and kaempferol) are also involved. Our results suggest that the presence of several biological active phytochemicals in noni juice enhances resistance to SARS-CoV-2 by interfering with its ability to bind ACE2. This is a new and significant anti-viral mechanism of noni juice that does not directly involve its immunomodulatory properties.

Antioxidant and antiangiogenic activities of the essential oils of Myristica fragrans and Morinda citrifolia

Objective:Toinvestigate the anti-angiogenic activity and antioxidant properties of Myristica fragrans（M.fragrans）（nutmeg） and Morinda citrifolia（M.citrifolia）（mengkudu） oils. Methods:The nutmeg and megkudu essential oils were obtained by steam distillation. The antioxidant activities of both essenlial oils were delermined by beta-carotene/ linoleic acid bleaching assay and reducing power while the anti-angiogenic activity was investigated using rat aortic ring assay using various concentrations.Results:The results showed that nutmeg oil has higher antioxidant activity than mengkudu oil.The nutmeg oil effectively inhibited the oxidation of linoleic acid with（88.68±0.1）%while the inhibition percentage of oxidation of linoleic acid of the mengkudu oil is（69.44±0.4）%.The nutmeg oil and mengkudu oil showed reducing power with an EC50 value of 181.4μg/mL and 3 043.0μg/mL,respectively.The anliangiogenic activity of nutmeg oil showed significant antiangiogenic activity with IC50 of 77.64μg/mL comparing to mengkudu oil which exhibits IC50 of 109.30μg/mL.Conclusion:Bioactive compound（s） will be isolated from the nutmeg essential oil to be developed as antiangiogenic drugs.

Effect of <i>Morinda citrifolia</i>L. fruit juice on gingivitis/periodontitis

The majority of the world’s population suffers from gingivitis/periodontitis. This inflammatory process is caused by several bacterial species inside the dental plaque. In vivo and in vitro experiments were set up. The patients of the in vivo group were divided into a noni and a control group. Both groups contained patients that suffered from gingivitis/periodontitis who were introduced to practice standardized, good oral hygiene. The patients in the noni group additionally used noni juice for mouth wash two times a day. The Papillae-Bleeding-Index (PBI) was evaluated comparing the status of inflammation in both groups. Bacterial probes were isolated from the patient’s gingival pouches for species identification and to carry out in vitro experiments for possible antimicrobial effects of noni juice. The Papillae-Bleeding-Index (PBI) in the noni group has “highly significantly” improved from an average of 2.25 at the beginning of the observation period (t0) to 1.01 after four weeks of noni treatment (t1), compared to a change from 2.11 at t0 to 1.95 at t1 inthe control group. A comparison of the differences of the PBI-values (t0-t1) between the noni and the control group was highly significant using a t-test on a level of p = 0.01. Only small inhibition zones were observed in the agar diffusion test on agar plates coated with aerobic, anaerobic and Candida cultures isolated from the patients gingival pouches after treatment with original or neutralized noni juice in different concentrations. Weak bacteriostatic effects occurred in the agar dilution experiments with noni juice in higher concentrations (native and neutralized noni juice). The present investigation has shown that the combination of good oral hygiene and the administration of noni juice was a promising treatment for gingivitis and periodontitis. The additional treatment with noni juice significantly mitigated the gingival inflammation.

<i>Morinda citrifolia</i>(Noni) Fruit Juice Inhibits Endocannabinoid Degradation Enzymes

Morinda citrifolia (noni) fruit juice has been shown to have a wide variety of potential health benefits in human clinical trials. It may also influence the endocannabinoid system of the body. Since the main ingredient of the product studied in these clinical trials was juice made from noni fruit puree from French Polynesia, it was evaluated for its ability to inhibit the two major endocannabinoid degradation enzymes in vitro. Noni fruit juice inhibited both fatty acid amide hydrolase (FAAH) and monoacylglycerol lipase (MAGL) in a concentration-dependent manner, suggesting that it may help maintain anandamide and 2-arachidonoylglycerol levels. Samples of the puree were also analyzed for the presence of characteristic phytochemical markers of authentic noni fruit such as scopoletin, rutin, quercetin, deacetylasperulosidic acid and asperulosidic acid, all of which were present. Also present was scandoside, which is reported for the first time as being identified in noni fruit or its juice. Some of these compounds may contribute to the FAAH and MAGL inhibiting activity of noni juice. These results reveal another set of mechanisms by which noni juice possibly supports mental health, maintains joint health, relieves discomfort and modulates the immune system.

The Influence of <i>Morinda citrifolia</i>(Noni) Fruit Juice on Collagen Deposition in the Skin: A Minireview

The fruit of Morinda citrifolia, commonly known as noni, has an extensive history of use as a food and medicine throughout the tropics. Among its many uses, noni was believed to promote skin health. In vivo wound healing studies reveal that noni fruit juice ingestion increases collagen production and deposition via increased expression of extracellular matrix protein genes. Noni juice also appears to promote fibroblast proliferation. In vitro studies indicate that noni juice also protects fibroblasts via activation of the transcription factor Nrf2 and protects extracellular matrix collagen by inhibiting matrix metalloproteinases. In vitro and in vivo study findings are corroborated by a clinical trial where hospitalization times were reduced, and wound granulation quality was improved in pediatric burn patients. The findings of other human studies also indicate that noni juice has the potential to protect skin collagen. These include reduced plasma reactive oxygen species in cigarette smokers, lowered skin glycation levels in overweight and obese adults, as well as reduced skin glycation levels among noni juice consumers within the general population. These in vitro, in vivo and human studies reveal that there are multiple mechanisms of action through which noni juice promotes collagen synthesis and inhibits collagen degradation. The procollagen properties of noni juice aid wound healing, increase skin elasticity, and improve the overall appearance of the skin.

Lignans from <i>Morinda citrifolia</i>(Noni) Fruit Inhibit Fatty Acid Amide Hydrolase and Monoacylglycerol Lipase <i>in Vitro</i>

Morinda citrifolia (noni) fruit juice has exhibited a variety of biological activities in human clinical trials, indicating that it influences multiple systems of the body. Since the 1990s, the endocannabinoid system (ECS) has been found to modulate the activity of other organ systems. To investigate noni’s potential impact on the ECS, extracts from freeze-dried noni fruit were evaluated in fatty acid amide hydrolase (FAAH) and monoacylglycerol lipase (MAGL) inhibition assays. The ethyl acetate extract demonstrated the greatest activity against both enzymes. Lignans in this extract also inhibited enzyme activities, with americanin A being the most active in both assays. Americanoic acid and 3,3’-bisdemethylpinoresinol were the next most active compounds. These results suggest that lignans in noni fruit may influence endocannabinoid levels within the body via FAAH and MAGL inhibition. This reveals another set of probable mechanisms of action by which noni juice affects human health.

Mineral and Trace Element Concentrations in <i>Morinda citrifolia</i>L. (Noni) Leaf, Fruit and Fruit Juice

Fruit and leaf of noni (Morinda citrifolia L.) have been used traditionally as food and for medicinal purposes by South Pacific populations since over two thousand years. Recently noni fruit juice became very popular as health beverage worldwide. Manufacturers and distributors of noni juice often praise the high content of minerals and trace elements in conjunction with the geological situation of the origin of the raw noni material. We therefore performed an investigation about the metal concentration of noni fruit, leaf and soil samples from 42 different locations in French Polynesia, and of 16 commercial noni juices. Median concentrations of metals in noni fruit, leaf and fruit juice appeared in a normal range compared to other fruits, however, the variance between different locations was considerable. Noni fruits from lime soil were poorer compared to volcanic soil in most metals. Self-prepared noni juices did generally not serve for more then 10% of the daily requirement of trace elements and minerals per 100 ml. The metal concentration in noni fruit puree was higher compared to clear juice and reached 10% and 34% of the daily requirement in 100 ml for manganese and chromium respectively. The concentrations of toxic metals (As, Cd, Pb) were below 2% of the maximum permitted levels (MPL) in all of these juices. For one of the commercial noni juices the MPL for lead was exceeded (170%) and some others were close to it. Most metals showed a good correlation regarding the concentration in noni fruit versus leaf, but not for soil versus fruit or juice. The concentration of magnesium, manganese, zinc and germanium in the commercial juices was highly correlated to the potassium concentration, indicating that these elements are useful to detect a dilution of noni juices.

Noni(Morinda citrifolia L.) fruit juice delays immunosenescence in the lymphocytes in lymph nodes of old F344 rats

Objective： Aging is associated with the development of diseases because of immunosuppression and altered functioning of the neuroendocrine system. The medicinal properties of Morinda citrifolia L. have been widely exploited for the treatment of age-associated diseases. This study aims to investigate the in vitro and in vivo effects of noni（M. citrifolia） fruit juice（NFJ） on neuro-immunomodulation in the lymph node lymphocytes of F344 rats.Methods： Lymphocytes isolated from axillary and inguinal lymph nodes of young（3–4 months） and old（18–21 months） rats were treated in vitro with different concentrations（0.0001%, 0.01%, and 1%） of NFJ for a period of 24 h. In the in vivo study, old（16–17 months） male F344 rats were treated with 5 mL/kg body weight of 5%, 10% and 20% of NFJ, twice a day, by oral gavage, and lymph node lymphocytes were isolated after 60 d. Concanavalin A（Con A）-induced lymphocyte proliferation, interleukin-2（IL-2）and interferon-γ（IFN-γ） production and expression of intracellular markers, such as phosphoextracellular signal-regulated kinase（p-ERK1/2）, phospho-γ AMP response element-binding protein,phospho-protein kinase B（p-Akt）, phospho-tyrosine hydroxylase（p-TH）, phospho-nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor-α（p-IκB-α） and phospho-nuclear factor-κB（p-NF-κB p65 and p50） were examined in the lymphocytes of lymph nodes.Results： NFJ increased Con A-induced lymphocyte proliferation, IL-2 and IFN-γ production, and p-ERK1/2 expression both in vitro and in vivo. In in vivo NFJ-treated old rats, lymph node lymphocytes showed increased expression of p-TH and Akt, nitric oxide production and decreased expression of p-NF-κB p65 and p50.Conclusion： These results suggest that the immunostimulatory properties of NFJ are facilitated through intracellular signaling pathways involving ERK1/2, Akt and NF-κB.

Morinda citrifolia(Noni) Juice Suppresses A549 Human Lung Cancer Cells via Inhibiting AKT/Nuclear Factor-κB Signaling Pathway

Objective:To study the mechanism of the anti-tumor effect of Morinda citrifolia(noni).Methods:The influences of noni juice on cell proliferation,apoptosis,invasion,migration and the activity of AKT/nuclear factor-κB(NF-κB)signaling pathway in A549 human lung cancer cells were detected by MTT,cell counting kit-8,colony formation,Annexin V/PI double labeling,transwel,scratch test and immunoblotting assay,respectively.A549 cells were inoculated into the right axilla of nude mice,followed by noni juice treatment.The body weight of the nude mice was weighed,and the tumor volume and weight were measured.Cel proliferation and expression of apoptosis-related proteins were measured by immunohistochemistry,and the activity of NF-κB signaling pathway was measured by immunoblotting.Results:The in vitro studies showed that noni juice inhibited the A549 cel s proliferation,migration and invasion.Noni juice also promoted cel s apoptosis in A549 cel s.Immunoblotting assay showed that the phosphorylation level of AKT,p50,and STAT3 proteins was inhibited to different extents after noni juice treatment.The in vivo studies showed that noni juice effectively suppressed tumor formation of A549 cells in nude mice.Noni juice treatment inhibited the expression of Ki67,PCNA,and Bcl-2 protein in the tumor;while promoted the expression of caspase-3 protein.Additionally,we also found that noni juice treatment could restrain the activity of AKT/NF-κB signaling pathway in the tumor tissue.Conclusion:Noni juice inhibited the proliferation of A549 lung cancer cells,induced apoptosis,and inhibited cel invasion and migration via regulating AKT/NF-κB signaling pathway.

盐胁迫对水培海巴戟幼苗生理特性的影响

为明确海巴戟幼苗对盐的耐受范围及盐胁迫对海巴戟幼苗生长的影响,以水培180d的海巴戟苗为材料,基本培养基为1/8 MS液体培养基,其中分别加入质量分数为0、0.2%、0.4%、0.6%、0.8%和1.0%的NaCl进行胁迫处理。结果表明:随着NaCl胁迫浓度的增加与胁迫时间的推移,海巴戟叶片相对含水量(relative water content,RWC)与生物量逐渐降低,可溶性蛋白(solubleprotein,SP)、丙二醛(MDA)的含量以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、过氧化物酶(POD)活性均表现为先增加后减少的趋势。海巴戟幼苗的保护酶活性随着NaCl胁迫浓度的增加与胁迫时间的推移发生显著变化,在NaCl浓度0.8%以下范围,海巴戟可以通过调节渗透物质和抗氧化物酶来减轻伤害,保证生长;当NaCl浓度在0.8%及以上范围时,保护酶系统与细胞膜受损严重,进而影响海巴戟生长。因此,海巴戟幼苗可以在0.8%以下NaCl浓度范围的区域试种。

诺丽对冠部牙本质粘接强度及纳米渗漏的影响

目的探讨诺丽(Morinda citrifolia juice,MCJ)联合乙二胺四乙酸盐(ethylene diaminete traacetic acid,EDTA)根管冲洗对冠部牙本质粘接强度及纳米渗漏的影响,并与临床上最常用的根管冲洗液次氯酸钠(sodi⁃um hypochlorite,NaClO)相比较,为临床应用提供参考。方法本研究已通过单位伦理审查委员会批准。选取63颗因正畸拔除的人前磨牙,去除表面釉质暴露牙本质平面后,根据冲洗液不同随机分为对照组(蒸馏水组)和6个实验组,即:A组2.5%NaClO、B组5.25%NaClO、C组6%MCJ、D组2.5%NaClO⁃17%EDTA、E组5.25%NaClO⁃17%EDTA和F组6%MCJ⁃17%EDTA(n=9),分别用相应冲洗液浸泡20 min后,在其表面分层堆积4 mm×4 mm×3 mm的Z350树脂块,每组选取6个样本,低速切割机将每个样本切割成1 mm×1 mm×8 mm的试件条,进行微拉伸粘接强度测试,随后在体视显微镜下观察断裂类型;每组剩余的3个样本经老化后切割成1 mm厚的薄片,进行界面纳米渗漏测试以及扫描电子显微镜下观察树脂⁃牙本质粘接界面。结果各组微拉伸粘接强度测试值均有显著性差异(P<0.05),对照组粘接强度最高。实验组中,B组粘接强度最低,以粘接界面破坏为主;F组粘接强度最高,以混合破坏为主。各组纳米渗漏值均有显著性差异(P<0.05),对照组纳米渗漏值最低。实验组中,B组纳米渗漏值最高、树脂突较细,F组纳米渗漏值最低、树脂突粗大且密集。结论NaClO浓度越高,冠部牙本质的粘接强度及边缘封闭性越差;MCJ联合EDTA根管冲洗对冠部牙本质的粘接强度和边缘封闭性的影响小于NaClO联合EDTA根管冲洗。

诺丽多糖复合膜的制备及性能研究

为开发出良好环保的可食性复合膜,该研究利用亚临界水萃取法从诺丽果中提取出天然的植物多糖,并以不同比例的诺丽多糖与壳聚糖共混从而制备出可食性诺丽多糖复合膜,并对其物理性质和光学性能进行了表征。将共混比例不同的复合膜进行30 d的土壤埋藏实验,并测试其耐油性能。结果表明,随着复合膜中诺丽多糖添加比例的增大,提高了复合膜的溶解度、阻氧率和抗拉伸强度,降低了复合膜的水蒸气透过率和断裂伸长率,膜的色度a^(*)、b^(*)、ΔE值和不透明度显著增加,而L^(*)值显著降低,复合膜表观良好。电镜观察到膜的形貌结构光滑致密,X衍射和热分析可知复合膜各组分良好相容,同时具有良好的土壤生物降解率和耐油性能。因此诺丽多糖复合膜有望作为良好的环保型可食性复合膜应用到食品包装材料中。

海巴戟果实软化相关基因McXTH的克隆和表达分析

【目的】了解诺丽果实软化规律,克隆木葡聚糖内转糖苷酶/水解酶(XTH)基因,初步明确其表达模式。【方法】用果实硬度计测量后熟阶段的诺丽果实在25℃放置及乙烯利处理两种状态下的硬度变化。从诺丽果实采后0~48 h转录组数据中筛选差异表达的XTH候选基因。通过生物信息学分析,克隆目的基因全长,并利用荧光定量PCR技术分析基因表达模式,同时用便携式乙烯测量仪测定内源乙烯释放量。【结果】后熟阶段的海巴戟果实采后表皮硬度随贮藏时间增加而下降,且乙烯利处理后的软化趋势较室温自然放置更为显著。从海巴戟果实采后0~48 h转录组数据中筛选出基因XTH(DN16278g1)的表达量高、差异大。该基因具有完整的开放阅读框(ORF),克隆测序后将其命名为McXTH,并将其提交Genbank获得登录号为ON512442。McXTH基因ORF全长1062 bp,共编码353个氨基酸,与其他植物中的XTH基因氨基酸序列的相似性为75%~88%。RT-qPCR结果和内源乙烯释放量表明,在海巴戟果实成熟阶段McXTH的表达量与同时期的内源乙烯释放量在趋势上大体相同。【结论】海巴戟果实的软化受乙烯和关键基因XTH调控,Mc XTH的克隆为进一步进行XTH基因在海巴戟果实成熟软化过程的功能研究夯实基础。

巴戟天寡糖联合帕罗西汀对老年抑郁症患者血清FGF-22及ACTH表达的影响

目的:研究巴戟天寡糖联合帕罗西汀对老年抑郁症患者血清成纤维细胞生长因子22(fibroblast growth factor-22,FGF-22)以及促肾上腺皮质激素(adrenocorticotropic hormone,ACTH)表达的影响。方法:将160例老年抑郁症患者随机分为对照组和研究组各80例,对照组患者每日口服帕罗西汀20 mg,研究组在对照组基础上口服巴戟天寡糖胶囊,每次0.3 g,每天2次。治疗6周后评估两组患者临床疗效;分别于治疗前及治疗后采用汉密尔顿焦虑自评量表(Hamilton anxiety scale,HAMA)评价患者焦虑状态,采用汉密尔顿抑郁量表(Hamilton depression scale,HAMD)评价患者抑郁状态,采用酶联免疫吸附法测定患者血清白细胞介素1β(interleukin-1β,IL-1β)、肿瘤坏死因子α(tumor necrosis factor α,TNF-α)、FGF-22以及ACTH表达水平;观察两组患者治疗期间不良反应发生情况。结果:研究组总有效率为90.00%(72/80),高于对照组的65.00%(52/80)(P<0.05)。治疗后两组患者HAMA及HAMD评分均较治疗前降低,研究组低于对照组(P<0.05)。治疗后两组患者血清IL-1β和TNF-α水平均较治疗前降低,研究组低于对照组(P<0.05)。治疗后两组患者血清FGF-22水平均较治疗前升高,ACTH水平均较治疗前降低,研究组改善更明显(P<0.05)。研究组不良反应发生率为3.75%,低于对照组的12.50%(P<0.05)。结论:巴戟天寡糖联合帕罗西汀可以改善抑郁症患者抑郁及焦虑状态,降低炎性因子IL-1β、TNF-α及血清ACTH水平,提高FGF-22水平,效果优于单用帕罗西汀,且不良反应发生率低。