Objective:To evaluate the antioxidant activity of aqueous extract of Atoringa oleifeta M.oleifera) young leaves by in vivo as well as in vitro assays.Methods:In vitro study included estimation of total phenolic,total ...Objective:To evaluate the antioxidant activity of aqueous extract of Atoringa oleifeta M.oleifera) young leaves by in vivo as well as in vitro assays.Methods:In vitro study included estimation of total phenolic,total ilavonol,total flavonoid and total antioxidant power(FRAP assay).Tn addition, in vivo study was done with the identified most effective dose of 200 nig/kg of its lyophilized powder on normal and diabetic rats.Its effect on different oxidative free radical scavenging enzymes,viz,superoxide dismutase(SOD),catalase(CAT),glutathione-S-transferase(GST),lipid peroxide(LPO) contents were measured.Results:Significant increase in activities of SOD.CAT, GST while,a decrease in LPO content was observed.Whereas,total phenolic,flavonoid and ilavonol contents in the extract were found to be 120 mg/g of CAK,40.5 mg/g of QEK and 12.12 mg/g of QE,respectively.On the other hand.FRAP assay results of M.oleifera leaves was(85.00±5.00)μM of Fe^+/g of extract powder.Conclusions:The significant antioxidant activities of M.oleifera leaves from both in vivo as well as in vitro studies suggests that the regular intake of its leaves through diet can protect normal as well as diabetic patients against oxidative damage.展开更多
Objective: To elucidate the molecular mechanisms of the potent anti-obesity effect of Moringa oleifera Lam.(M. oleifera) ethanolic extract and to clarify the link between these mechanisms and the associated metabolic ...Objective: To elucidate the molecular mechanisms of the potent anti-obesity effect of Moringa oleifera Lam.(M. oleifera) ethanolic extract and to clarify the link between these mechanisms and the associated metabolic and vascular risks in the experimental model of visceral obesity.Methods: M. oleifera ethanolic extract was orally administered at 600 mg/kg body weight in obese female rats daily for 12 weeks. At the end of treatment, body weight was determined, and the atherogenic index, coronary artery index, glucose level, insulin resistance status, liver and kidney functions were assessed. Also, the m RNA of leptin, adiponectin and resistin in visceral adipose tissue was determined by quantitative real time-PCR.Results: The results showed that M. oleifera extract down-regulated m RNA expression of leptin and resistin, while it up-regulated adiponectin gene expression in obese rats relative to untreated obese control counterparts. This amelioration of genes expression was paralleled by a reduction in body weight and improvement of the atherogenic index and coronary artery index, as well as glucose level and insulin resistance value without adverse effects on liver or kidney functions, versus the untreated obese control ones.Conclusions: It is reasonable to assume that the anti-obesity, anti-atherogenic and antidiabetic properties of M. oleifera are mechanistically achieved via working directly on the adipokines of the visceral adipose tissue. Therefore, M. oleifera may be a good therapeutic candidate for the symptoms of metabolic syndrome.展开更多
Due to its unique economic value,Moringa oleifera Lam. has become a tree species with great development potential. This paper firstly introduced the value of development and utilization of M. oleifera in nutrition,med...Due to its unique economic value,Moringa oleifera Lam. has become a tree species with great development potential. This paper firstly introduced the value of development and utilization of M. oleifera in nutrition,medicinal,industrial,greening,etc. Then,focusing on the current development status of M. oleifera industry in Guangxi and combining Guangxi's resource conditions,it came up with recommendations for the comprehensive development and rational utilization of M. oleifera. Besides,it made a prediction of the prospect of Se-enriched M.oleifera industry in Guangxi.展开更多
Carbon tetrachloride(CCl4)is a hepatotoxin that triggers liver damage.This study aimed to evaluate the protective effect of phytochemicals detected in Moringa oleifera Lam.leaf extract(MOLE)on CCl4-induced hepatotoxic...Carbon tetrachloride(CCl4)is a hepatotoxin that triggers liver damage.This study aimed to evaluate the protective effect of phytochemicals detected in Moringa oleifera Lam.leaf extract(MOLE)on CCl4-induced hepatotoxicity in mice.Phytochemicals,total phenolics,and total fl avonoids were detected in MOLE.MOLE markedly decreased the elevation of serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in consistence with the ameliorating effect on CCl4-induced histopathological abnormalities.Moreover,MOLE significantly alleviated the decrease in the antioxidant defense mechanism induced by CCl4.The suppressing effect of MOLE on the boosted inflammatory pathway triggered by CCl4 was detected by measuring the protein levels of nuclear factor kappa-light-chain-enhancer of activated B-cells(NF-κB-p65),toll-like receptor 4(TLR4),tumor necrosis factor-α(TNF-α),interleukin(IL)-6,IL-1β,and IL-8 as well as the relative expressions of nuclear factor kappa B(NF-κB),TNF-α,IL-1β,and TLR4 genes.Apoptosis and genotoxicity induced by CCl4 were signifi cantly alleviated by MOLE.MOLE co-administration modulated TLR4/NF-κB pathway as presented by the suppressed gene expression of TLR4 and NF-κB as well as by the reduced protein expression of TLR4 and NF-κB-p65.In conclusion,MOLE has a multifarious protective role against hepatotoxicity through control of oxidative stress and modulation of TLR4/NF-κB.展开更多
Objective:To investigate the acute and delayed anti-inflammatory effects of Morning oleifera lam（MOL） crude methanolic extract.Methods:Compared the anti-inflammatory effects of MOL with that of standard anti-inflamm...Objective:To investigate the acute and delayed anti-inflammatory effects of Morning oleifera lam（MOL） crude methanolic extract.Methods:Compared the anti-inflammatory effects of MOL with that of standard anti-inflammatory agents like indomethacin and hydrocortisone using Air Pouch Model.Results:In both acute and delayed inflammation,the MOL extract produced dose dependent anti-inflammatory effect[acute IC<sub>50</sub>=（399.30±5.43） mg/kg;delayed IC<sub>50</sub>=（510.26±4.53） mg/kg].The order of anti-inflammatory potency for the three drugs was hydrocortisone】 indomethacin 】 MOL.Conclusions:These observations indicate that MOL possesses potential anti-inflammatory property.展开更多
基于Web of Science(简称WOS)核心合集和中文CNKI数据库收录的辣木生物活性研究相关文献,借助Citespace(5.8R3版本)可视化计量工具,对2000~2021年收录的文献进行了可视化分析和图像处理。首次回顾了二十年间辣木生物活性的研究历程和进...基于Web of Science(简称WOS)核心合集和中文CNKI数据库收录的辣木生物活性研究相关文献,借助Citespace(5.8R3版本)可视化计量工具,对2000~2021年收录的文献进行了可视化分析和图像处理。首次回顾了二十年间辣木生物活性的研究历程和进展,并跟踪分析其年度文献分布、研究机构、国家/地区分布等情况;通过关键词和文献共被引聚类分析了国内外研究热点的时间变化趋势。通过文献调研发现,辣木在防治代谢性疾病上有着显著的优势,并就此较详尽地综述了其研究现状与进展。通过活性可视化分析,旨在使国内科研工作者较全面了解辣木生物活性研究的较新动态,关注和预测未来研究热点可能的发展趋势,为推动辣木高值化利用提供参考信息。展开更多
Diabetes mellitus(DM)is a chronic metabolic disease characterized by hyperglycemia,which poses a serious threat to human health.Moringa oleifera Lam.is a medicinal and edible plant with various physiological functions...Diabetes mellitus(DM)is a chronic metabolic disease characterized by hyperglycemia,which poses a serious threat to human health.Moringa oleifera Lam.is a medicinal and edible plant with various physiological functions.However,its main hypoglycemic components and mechanisms are still unclear.In this study,network pharmacology and bioinformatics were used to analyze the potential bioactive substances of M.oleifera leaf extract(MOLE)and its hypoglycemic mechanism.Studies have shown that MOLE has the effect of increasing glucose consumption in insulin resistant-HepG2 cells.MOLE was found to contain 975 compounds by ultrahigh performance liquid chromatography-mass spectrometry(UHPLC-MS).Network pharmacology analysis indicated that the main active component was robinetin and the identified core genes were AKT1 and GAPDH.KEGG pathway enrichment analysis showed that the hypoglycemic effect of MOLE may be closely related to the PI3K-Akt signaling pathway.This study revealed the possible active components and mechanisms of action of M.oleifera for hypoglycemia,laying the theoretical foundation for subsequent studies.展开更多
基金National Medicinal Plants Board. Govt,of India,New Delhi,India for providing financial assistance in the form of Senior Research Fellowship to cam' out the present study
文摘Objective:To evaluate the antioxidant activity of aqueous extract of Atoringa oleifeta M.oleifera) young leaves by in vivo as well as in vitro assays.Methods:In vitro study included estimation of total phenolic,total ilavonol,total flavonoid and total antioxidant power(FRAP assay).Tn addition, in vivo study was done with the identified most effective dose of 200 nig/kg of its lyophilized powder on normal and diabetic rats.Its effect on different oxidative free radical scavenging enzymes,viz,superoxide dismutase(SOD),catalase(CAT),glutathione-S-transferase(GST),lipid peroxide(LPO) contents were measured.Results:Significant increase in activities of SOD.CAT, GST while,a decrease in LPO content was observed.Whereas,total phenolic,flavonoid and ilavonol contents in the extract were found to be 120 mg/g of CAK,40.5 mg/g of QEK and 12.12 mg/g of QE,respectively.On the other hand.FRAP assay results of M.oleifera leaves was(85.00±5.00)μM of Fe^+/g of extract powder.Conclusions:The significant antioxidant activities of M.oleifera leaves from both in vivo as well as in vitro studies suggests that the regular intake of its leaves through diet can protect normal as well as diabetic patients against oxidative damage.
基金Supported by Science & Technology Development Fund in Egypt project entitled “Recent approaches in the utilization of M.oleifera and Moringa peregrina as a good nutritional,medicinal and industrial plant in Egypt”(Grant No.5979)
文摘Objective: To elucidate the molecular mechanisms of the potent anti-obesity effect of Moringa oleifera Lam.(M. oleifera) ethanolic extract and to clarify the link between these mechanisms and the associated metabolic and vascular risks in the experimental model of visceral obesity.Methods: M. oleifera ethanolic extract was orally administered at 600 mg/kg body weight in obese female rats daily for 12 weeks. At the end of treatment, body weight was determined, and the atherogenic index, coronary artery index, glucose level, insulin resistance status, liver and kidney functions were assessed. Also, the m RNA of leptin, adiponectin and resistin in visceral adipose tissue was determined by quantitative real time-PCR.Results: The results showed that M. oleifera extract down-regulated m RNA expression of leptin and resistin, while it up-regulated adiponectin gene expression in obese rats relative to untreated obese control counterparts. This amelioration of genes expression was paralleled by a reduction in body weight and improvement of the atherogenic index and coronary artery index, as well as glucose level and insulin resistance value without adverse effects on liver or kidney functions, versus the untreated obese control ones.Conclusions: It is reasonable to assume that the anti-obesity, anti-atherogenic and antidiabetic properties of M. oleifera are mechanistically achieved via working directly on the adipokines of the visceral adipose tissue. Therefore, M. oleifera may be a good therapeutic candidate for the symptoms of metabolic syndrome.
基金Supported by Scientific Research and Technological Development Program of Xixiangtang District of Nanning City(201710304)Key Research and Development Program of Guangxi(Gui Ke AB16380088)+5 种基金Guangxi Se-enriched Crop Experiment Station(Gui TS2016011)Key Research and Development Program of Qingxiu District of Nanning City(2016039)Scientific Development Fund Project of Guangxi Academy of Agricultural Sciences(2017JM01,2017JM03,2015JM23)Technological Achievement Transformation Project of Guangxi Academy of Agricultural Sciences(2017NZ04)Key Science and Technology Project of Guangxi(Gui Ke AA17202026-2)Special Fund Project for Innovation Driven Development of Guangxi(Gui Ke AA17202019,Gui Ke AA17202019-4,and Gui Ke AA17202019-1)
文摘Due to its unique economic value,Moringa oleifera Lam. has become a tree species with great development potential. This paper firstly introduced the value of development and utilization of M. oleifera in nutrition,medicinal,industrial,greening,etc. Then,focusing on the current development status of M. oleifera industry in Guangxi and combining Guangxi's resource conditions,it came up with recommendations for the comprehensive development and rational utilization of M. oleifera. Besides,it made a prediction of the prospect of Se-enriched M.oleifera industry in Guangxi.
文摘Carbon tetrachloride(CCl4)is a hepatotoxin that triggers liver damage.This study aimed to evaluate the protective effect of phytochemicals detected in Moringa oleifera Lam.leaf extract(MOLE)on CCl4-induced hepatotoxicity in mice.Phytochemicals,total phenolics,and total fl avonoids were detected in MOLE.MOLE markedly decreased the elevation of serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in consistence with the ameliorating effect on CCl4-induced histopathological abnormalities.Moreover,MOLE significantly alleviated the decrease in the antioxidant defense mechanism induced by CCl4.The suppressing effect of MOLE on the boosted inflammatory pathway triggered by CCl4 was detected by measuring the protein levels of nuclear factor kappa-light-chain-enhancer of activated B-cells(NF-κB-p65),toll-like receptor 4(TLR4),tumor necrosis factor-α(TNF-α),interleukin(IL)-6,IL-1β,and IL-8 as well as the relative expressions of nuclear factor kappa B(NF-κB),TNF-α,IL-1β,and TLR4 genes.Apoptosis and genotoxicity induced by CCl4 were signifi cantly alleviated by MOLE.MOLE co-administration modulated TLR4/NF-κB pathway as presented by the suppressed gene expression of TLR4 and NF-κB as well as by the reduced protein expression of TLR4 and NF-κB-p65.In conclusion,MOLE has a multifarious protective role against hepatotoxicity through control of oxidative stress and modulation of TLR4/NF-κB.
文摘Objective:To investigate the acute and delayed anti-inflammatory effects of Morning oleifera lam（MOL） crude methanolic extract.Methods:Compared the anti-inflammatory effects of MOL with that of standard anti-inflammatory agents like indomethacin and hydrocortisone using Air Pouch Model.Results:In both acute and delayed inflammation,the MOL extract produced dose dependent anti-inflammatory effect[acute IC<sub>50</sub>=（399.30±5.43） mg/kg;delayed IC<sub>50</sub>=（510.26±4.53） mg/kg].The order of anti-inflammatory potency for the three drugs was hydrocortisone】 indomethacin 】 MOL.Conclusions:These observations indicate that MOL possesses potential anti-inflammatory property.
文摘基于Web of Science(简称WOS)核心合集和中文CNKI数据库收录的辣木生物活性研究相关文献,借助Citespace(5.8R3版本)可视化计量工具,对2000~2021年收录的文献进行了可视化分析和图像处理。首次回顾了二十年间辣木生物活性的研究历程和进展,并跟踪分析其年度文献分布、研究机构、国家/地区分布等情况;通过关键词和文献共被引聚类分析了国内外研究热点的时间变化趋势。通过文献调研发现,辣木在防治代谢性疾病上有着显著的优势,并就此较详尽地综述了其研究现状与进展。通过活性可视化分析,旨在使国内科研工作者较全面了解辣木生物活性研究的较新动态,关注和预测未来研究热点可能的发展趋势,为推动辣木高值化利用提供参考信息。
基金supported by the Major Project of Science and Technology Department of Yunnan Province(202002AA100005 and 202102AE090027-2)Cassava Industrial Technology System of China(CARS-11-YNTY)Yunnan Province Ten Thousand Plan Industrial Technology Talents project(YNWR-CYJS-2020-010).
文摘Diabetes mellitus(DM)is a chronic metabolic disease characterized by hyperglycemia,which poses a serious threat to human health.Moringa oleifera Lam.is a medicinal and edible plant with various physiological functions.However,its main hypoglycemic components and mechanisms are still unclear.In this study,network pharmacology and bioinformatics were used to analyze the potential bioactive substances of M.oleifera leaf extract(MOLE)and its hypoglycemic mechanism.Studies have shown that MOLE has the effect of increasing glucose consumption in insulin resistant-HepG2 cells.MOLE was found to contain 975 compounds by ultrahigh performance liquid chromatography-mass spectrometry(UHPLC-MS).Network pharmacology analysis indicated that the main active component was robinetin and the identified core genes were AKT1 and GAPDH.KEGG pathway enrichment analysis showed that the hypoglycemic effect of MOLE may be closely related to the PI3K-Akt signaling pathway.This study revealed the possible active components and mechanisms of action of M.oleifera for hypoglycemia,laying the theoretical foundation for subsequent studies.
