Light-sheet fluorescence microscopy(LSFM)has been widely used to image the three-dimensional(3D)structures and functions of various millimeter-size bio-specimen such as zebrafish.However,the sample adsorption and scat...Light-sheet fluorescence microscopy(LSFM)has been widely used to image the three-dimensional(3D)structures and functions of various millimeter-size bio-specimen such as zebrafish.However,the sample adsorption and scattering cause shading of the light-sheet illumination,preventing the even 3D image of thick samples.Herein,we report a continuous-rotational light-sheet microscope(CR-LSM)that enables simultaneous 3D bright-field and fluorescence imaging.With a high-accuracy rotational stage,CR-LSM records the outline projections and the fluorescent images of the sample at multiple rotation angles.Then,3D morphology and fluorescent structure were reconstructed with a developed algorithm.Using CR-LSM,zebrafish’s whole-fish contour and blood vessel structures were obtained simultaneously.展开更多
BACKGROUND At present,there is controversy on the role of microvessel density(MVD)in tumors as a prognostic indicator of bladder transitional cell carcinoma(BTCC).However,the MVD in tumors is simply classified based o...BACKGROUND At present,there is controversy on the role of microvessel density(MVD)in tumors as a prognostic indicator of bladder transitional cell carcinoma(BTCC).However,the MVD in tumors is simply classified based on the expression of several different vascular markers,which has not been related to analytical research on the prognosis of patients with BTCC.AIM To explore the classification of blood vessels in tumors and studied the relationship between MVD and the prognosis of patients with BTCC.METHODS The tissue mass was detected by tissue microarray and immunohistochemical analysis with monoclonal antibodies against CD31,CD34,CD105,and vascular smooth muscle actin to investigate the MVD in BTCC.The measurement data are expressed as the mean±SD.The difference between the groups was analyzed by the t-test,the counting data were analyzed byχ2 test.The Kaplan-Meier survival curve was estimated by the product-limit method.The log-rank time-series test was employed to compare the tumor-free survival curves.RESULTS The MVD was closely related to the pathological grade,invasive depth,and prognosis of BTCC.Significant differences were found between grade I and grade II,grade II and grade III,superficial and invasive type,and the tumor-free survival group and the recurrence or metastasis group(P<0.01).Multivariate analysis showed that undifferentiated MVD was an independent prognostic factor for patient survival time.An inverse correlation between undifferentiated tumor MVD and differentiated tumor MVD in BTCC was also shown.CONCLUSION The classification of blood vessels in BTCC could act as an important prognostic indicator and may also be of great significance in the treatment of cancer.展开更多
Angiogenesis is the proliferation of new blood vessels that enable the normal embryonic development and may also induce growth of numerous pathologies like tumor growth and cancer metastasis.The assays were used for b...Angiogenesis is the proliferation of new blood vessels that enable the normal embryonic development and may also induce growth of numerous pathologies like tumor growth and cancer metastasis.The assays were used for both pro and anti-angiogenic agents.This study evaluated the angiosuppressive activity of Pleurotus florida on the developing chick embryo using chorioallantoic membrane(CAM)assay and used destructive sampling into four different treatment concentrations and a control.The result revealed a decrease in the number of blood vessels branch points formed after 24,48,and 72 hours at higher treatment concentrations(0.50%and 1%).Statistical analysis showed that 24 hours and 48 hours of exposure to various concentrations have no significant difference.Nevertheless,after 72 hours of exposure,the mean blood vessel branch points formed showed a significant difference among the treatment means between the highest concentration of P.florida(1%)and the control treatment(sterile water).In terms of the percentage of angiosuppressive activity of P.florida with the greatest angiogenic effect,which was the 1%P.florida treatment concentration possesses 35.69%and 36.85%angiosuppressive rate after 48 and 72 hours of exposure respectively.Thus,this study showed that P.florida lyophilized hot water extract exhibits angiosuppressive activity on the developing chick embryo using chorioallantoic membrane assay and is concentration and time exposure dependent.展开更多
基金the National Natural Science Foundation of China(62205368)the Key Research and Development Program of Jiangsu Province(BE2020664).
文摘Light-sheet fluorescence microscopy(LSFM)has been widely used to image the three-dimensional(3D)structures and functions of various millimeter-size bio-specimen such as zebrafish.However,the sample adsorption and scattering cause shading of the light-sheet illumination,preventing the even 3D image of thick samples.Herein,we report a continuous-rotational light-sheet microscope(CR-LSM)that enables simultaneous 3D bright-field and fluorescence imaging.With a high-accuracy rotational stage,CR-LSM records the outline projections and the fluorescent images of the sample at multiple rotation angles.Then,3D morphology and fluorescent structure were reconstructed with a developed algorithm.Using CR-LSM,zebrafish’s whole-fish contour and blood vessel structures were obtained simultaneously.
文摘BACKGROUND At present,there is controversy on the role of microvessel density(MVD)in tumors as a prognostic indicator of bladder transitional cell carcinoma(BTCC).However,the MVD in tumors is simply classified based on the expression of several different vascular markers,which has not been related to analytical research on the prognosis of patients with BTCC.AIM To explore the classification of blood vessels in tumors and studied the relationship between MVD and the prognosis of patients with BTCC.METHODS The tissue mass was detected by tissue microarray and immunohistochemical analysis with monoclonal antibodies against CD31,CD34,CD105,and vascular smooth muscle actin to investigate the MVD in BTCC.The measurement data are expressed as the mean±SD.The difference between the groups was analyzed by the t-test,the counting data were analyzed byχ2 test.The Kaplan-Meier survival curve was estimated by the product-limit method.The log-rank time-series test was employed to compare the tumor-free survival curves.RESULTS The MVD was closely related to the pathological grade,invasive depth,and prognosis of BTCC.Significant differences were found between grade I and grade II,grade II and grade III,superficial and invasive type,and the tumor-free survival group and the recurrence or metastasis group(P<0.01).Multivariate analysis showed that undifferentiated MVD was an independent prognostic factor for patient survival time.An inverse correlation between undifferentiated tumor MVD and differentiated tumor MVD in BTCC was also shown.CONCLUSION The classification of blood vessels in BTCC could act as an important prognostic indicator and may also be of great significance in the treatment of cancer.
文摘Angiogenesis is the proliferation of new blood vessels that enable the normal embryonic development and may also induce growth of numerous pathologies like tumor growth and cancer metastasis.The assays were used for both pro and anti-angiogenic agents.This study evaluated the angiosuppressive activity of Pleurotus florida on the developing chick embryo using chorioallantoic membrane(CAM)assay and used destructive sampling into four different treatment concentrations and a control.The result revealed a decrease in the number of blood vessels branch points formed after 24,48,and 72 hours at higher treatment concentrations(0.50%and 1%).Statistical analysis showed that 24 hours and 48 hours of exposure to various concentrations have no significant difference.Nevertheless,after 72 hours of exposure,the mean blood vessel branch points formed showed a significant difference among the treatment means between the highest concentration of P.florida(1%)and the control treatment(sterile water).In terms of the percentage of angiosuppressive activity of P.florida with the greatest angiogenic effect,which was the 1%P.florida treatment concentration possesses 35.69%and 36.85%angiosuppressive rate after 48 and 72 hours of exposure respectively.Thus,this study showed that P.florida lyophilized hot water extract exhibits angiosuppressive activity on the developing chick embryo using chorioallantoic membrane assay and is concentration and time exposure dependent.
