Arachidonic acid （ARA or AA）, one of the most important polyunsaturated fatty acids （PUFAs）, has various physiological activities and positive effects on human health. ARA production by Mortierella alpina has beco...Arachidonic acid （ARA or AA）, one of the most important polyunsaturated fatty acids （PUFAs）, has various physiological activities and positive effects on human health. ARA production by Mortierella alpina has become a hot topic in recent years, owing to that it is effective, safe and easy to control. How to improve ARA yield and purification efficiency is important to ARA production in M. alpina. Therefore, in this review, we summarized some methods to improve ARA yield： optimization of culture conditions, mycelium aging technologies and metabolic regulation, and the commonly used methods for ARA isolation and purification, to provide a theoretical basis for ARA production by M. alpina fermentation.展开更多
The analysis of flux distributions in metabolic networks has become an important approach for understand-ing the fermentation characteristics of the process.A model of metabolic flux analysis of arachidonic acid(AA)sy...The analysis of flux distributions in metabolic networks has become an important approach for understand-ing the fermentation characteristics of the process.A model of metabolic flux analysis of arachidonic acid(AA)synthesis in Mortierella alpina ME-1 was established and carbon flux distributions were estimated in different fermentation phases with different concentrations of N-source.During the expo-nential,decelerating and stationary phase,carbon fluxes to AA were 3.28%,8.80%and 6.97%,respectively,with sufficient N-source broth based on the flux of glucose uptake,and those were increased to 3.95%,19.21%and 39.29%,respectively,by regulating the shifts of carbon fluxes via fermentation with limited N-source broth and adding 0.05% NaNO_(3) at 96 h.Eventually AA yield was increased from 1.3 to 3.5 g·L^(−1).These results suggest a way to improve AA fermentation,that is,fermentation with limited N-source broth and adding low concentration N-source during the stationary phase.展开更多
在单因素试验基础上,采用响应面分析法优化深黄被孢霉突变菌株发酵制备花生四烯酸工艺,确定最优发酵工艺条件为:接种量15.66%,发酵温度28.29℃,发酵时间6.58 d,发酵p H 5.96。花生四烯酸产量为3.12 g·L-1,在最优发酵工艺条件下,可...在单因素试验基础上,采用响应面分析法优化深黄被孢霉突变菌株发酵制备花生四烯酸工艺,确定最优发酵工艺条件为:接种量15.66%,发酵温度28.29℃,发酵时间6.58 d,发酵p H 5.96。花生四烯酸产量为3.12 g·L-1,在最优发酵工艺条件下,可提高产量,同时减少接种量,缩短发酵时间,降低生产成本。展开更多
基金Supported by Natural Science Foundation of Yunnan Province(2016FB030)Young Academic and Technical Talent Program of Yunnan Province(2011CI027)~~
文摘Arachidonic acid （ARA or AA）, one of the most important polyunsaturated fatty acids （PUFAs）, has various physiological activities and positive effects on human health. ARA production by Mortierella alpina has become a hot topic in recent years, owing to that it is effective, safe and easy to control. How to improve ARA yield and purification efficiency is important to ARA production in M. alpina. Therefore, in this review, we summarized some methods to improve ARA yield： optimization of culture conditions, mycelium aging technologies and metabolic regulation, and the commonly used methods for ARA isolation and purification, to provide a theoretical basis for ARA production by M. alpina fermentation.
基金This work was supported by the National Natural Science Foundation of China(Grant No.20576054)Natural Science Foundation of Jiangsu(Grant No.BK2005114)Jiangsu Planned Projects for Postdoctoral Research Funds.
文摘The analysis of flux distributions in metabolic networks has become an important approach for understand-ing the fermentation characteristics of the process.A model of metabolic flux analysis of arachidonic acid(AA)synthesis in Mortierella alpina ME-1 was established and carbon flux distributions were estimated in different fermentation phases with different concentrations of N-source.During the expo-nential,decelerating and stationary phase,carbon fluxes to AA were 3.28%,8.80%and 6.97%,respectively,with sufficient N-source broth based on the flux of glucose uptake,and those were increased to 3.95%,19.21%and 39.29%,respectively,by regulating the shifts of carbon fluxes via fermentation with limited N-source broth and adding 0.05% NaNO_(3) at 96 h.Eventually AA yield was increased from 1.3 to 3.5 g·L^(−1).These results suggest a way to improve AA fermentation,that is,fermentation with limited N-source broth and adding low concentration N-source during the stationary phase.
文摘在单因素试验基础上,采用响应面分析法优化深黄被孢霉突变菌株发酵制备花生四烯酸工艺,确定最优发酵工艺条件为:接种量15.66%,发酵温度28.29℃,发酵时间6.58 d,发酵p H 5.96。花生四烯酸产量为3.12 g·L-1,在最优发酵工艺条件下,可提高产量,同时减少接种量,缩短发酵时间,降低生产成本。
