This study aims to evaluate the preventive effects of anthocyanins extracts (MAEs) from mulberry variety PR-01 against N-nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis in rats. It was found that 150 mg·k...This study aims to evaluate the preventive effects of anthocyanins extracts (MAEs) from mulberry variety PR-01 against N-nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis in rats. It was found that 150 mg·kg-1 MAEs treatment significantly reduced the NDEA-induced hepatic nodules incidence and hepatocellular carcinoma incidence by 58.30% and 41.70% compared to the model group. Meanwhile, MAEs significantly restored the elevated the liver function enzymes, inhibited the tumor necrosis factor alpha and interleukin-6 levels, elevated the serum interleukin-10 and interferon-γ and increased hepatic glutathione-S-transferase and UDP-glucuronosyltransferase 2B1 enzyme activity. Moreover, 150 mg·kg-1 MAEs supplement enhanced glutathione content and the activities of superoxide dismutase, catalase, glutathione peroxidase activities but reduced the malondialdehyde and thiobarbituric acid-reactive substances content by 37.90% and 44.52%. Furthermore, MAEs pretreatment maintained nuclear factor erythroid 2-related factor 2 (Nrf2), Kelch-like ECH-associated protein 1, heme oxygenase-1, and NAD(P)H: quinine oxidoreductase1 stimulation and inhibited the expression of TNF-α, nuclear factor-kappaB (NF-κB), and cyclooxygenase-2 (COX-2), indicating that MAEs exhibit effectively prevention effects against liver cancer via decreased lipid peroxidation, induced Nrf2-mediated antioxidant enzymes and attenuating the inflammatory mediators COX-2 through NF-κB pathway. Thus, MAEs of mulberry variety PR-01 may be used as a good functional dietary supplement against liver cancer.展开更多
以95%乙醇为溶剂,采用超声法提取桑白皮活性成分黄酮,以黄酮提取率为评价指标,在单因素实验的基础上,采用响应面法优化了提取工艺,并研究了桑白皮黄酮提取液的体外抗氧化活性。结果表明,桑白皮黄酮的最优提取条件为:提取温度45.78℃、...以95%乙醇为溶剂,采用超声法提取桑白皮活性成分黄酮,以黄酮提取率为评价指标,在单因素实验的基础上,采用响应面法优化了提取工艺,并研究了桑白皮黄酮提取液的体外抗氧化活性。结果表明,桑白皮黄酮的最优提取条件为:提取温度45.78℃、提取时间38.33 min、料液比1∶25.52(g∶mL),在此条件下,黄酮提取率为2.211%。体外抗氧化实验表明,2.00 mL 0.50 mg·mL^(-1)桑白皮黄酮提取液对4.00 mL 0.05 mg·mL^(-1)DPPH自由基的清除率为62.21%,低于同浓度的阳性对照VC(清除率81.39%);抗氧化实验的精密度、稳定性、重复性良好,RSD值分别为0.05%、0.15%、0.69%(n=5)。该提取方法简单、高效、可靠,为桑白皮的后续开发提供了一定理论基础。展开更多
文摘This study aims to evaluate the preventive effects of anthocyanins extracts (MAEs) from mulberry variety PR-01 against N-nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis in rats. It was found that 150 mg·kg-1 MAEs treatment significantly reduced the NDEA-induced hepatic nodules incidence and hepatocellular carcinoma incidence by 58.30% and 41.70% compared to the model group. Meanwhile, MAEs significantly restored the elevated the liver function enzymes, inhibited the tumor necrosis factor alpha and interleukin-6 levels, elevated the serum interleukin-10 and interferon-γ and increased hepatic glutathione-S-transferase and UDP-glucuronosyltransferase 2B1 enzyme activity. Moreover, 150 mg·kg-1 MAEs supplement enhanced glutathione content and the activities of superoxide dismutase, catalase, glutathione peroxidase activities but reduced the malondialdehyde and thiobarbituric acid-reactive substances content by 37.90% and 44.52%. Furthermore, MAEs pretreatment maintained nuclear factor erythroid 2-related factor 2 (Nrf2), Kelch-like ECH-associated protein 1, heme oxygenase-1, and NAD(P)H: quinine oxidoreductase1 stimulation and inhibited the expression of TNF-α, nuclear factor-kappaB (NF-κB), and cyclooxygenase-2 (COX-2), indicating that MAEs exhibit effectively prevention effects against liver cancer via decreased lipid peroxidation, induced Nrf2-mediated antioxidant enzymes and attenuating the inflammatory mediators COX-2 through NF-κB pathway. Thus, MAEs of mulberry variety PR-01 may be used as a good functional dietary supplement against liver cancer.
文摘以95%乙醇为溶剂,采用超声法提取桑白皮活性成分黄酮,以黄酮提取率为评价指标,在单因素实验的基础上,采用响应面法优化了提取工艺,并研究了桑白皮黄酮提取液的体外抗氧化活性。结果表明,桑白皮黄酮的最优提取条件为:提取温度45.78℃、提取时间38.33 min、料液比1∶25.52(g∶mL),在此条件下,黄酮提取率为2.211%。体外抗氧化实验表明,2.00 mL 0.50 mg·mL^(-1)桑白皮黄酮提取液对4.00 mL 0.05 mg·mL^(-1)DPPH自由基的清除率为62.21%,低于同浓度的阳性对照VC(清除率81.39%);抗氧化实验的精密度、稳定性、重复性良好,RSD值分别为0.05%、0.15%、0.69%(n=5)。该提取方法简单、高效、可靠,为桑白皮的后续开发提供了一定理论基础。