[Objective] The study aimed at analyzing the genetic relationship of 64 local varieties of Morus atropurpurea Roxb.from the Pearl River Basin in Guangdong and Guangxi Provinces.[Method] Genetic diversity of 64 local v...[Objective] The study aimed at analyzing the genetic relationship of 64 local varieties of Morus atropurpurea Roxb.from the Pearl River Basin in Guangdong and Guangxi Provinces.[Method] Genetic diversity of 64 local varieties of Morus atropurpurea Roxb.was analyzed by ISSR molecular marker technique.The genetic relationship among these local varieties was researched by UPGMA method based on the genetic similarity coefficient.[Result] 128 bands were amplified from the total DNA of 64 local varieties using 13 ISSR primers,of which 109 bands accounting for 85.15% were polymorphic.It meant that there was rich genetic diversity among the local varieties tested.The genetic similarity coefficients among 64 local varieties were relatively high with a range of 0.500 0-0.929 7.In addition,64 local varieties were divided into two categories and the second could be further divided into 10 subcategories.It was suggested that the genetic relationship of 64 local varieties of Morus atropurpurea Roxb.based on ISSR marker analysis has a certain correlation with geographical distribution.[Conclusion] ISSR marker technology was suitable for evaluating genetic relationship and genetic diversity of local varieties of Morus atropurpurea Roxb.in Pearl River Basin,and could provide scientific basis for DNA fingerprinting and identification of varieties of Morus atropurpurea Roxb.展开更多
【目的】针对桑树形态倍性鉴定及品种间鉴别问题，研究广东桑不同倍性及品种之间孢粉学上的异同，为桑树多倍体育种提供科学依据。【方法】利用扫描电镜对广东桑10个不同倍性品种花粉的形态、大小、萌发孔、外壁纹饰等孢粉学特性进行观...【目的】针对桑树形态倍性鉴定及品种间鉴别问题，研究广东桑不同倍性及品种之间孢粉学上的异同，为桑树多倍体育种提供科学依据。【方法】利用扫描电镜对广东桑10个不同倍性品种花粉的形态、大小、萌发孔、外壁纹饰等孢粉学特性进行观察，并运用图片分析结合数理统计的方法对其花粉形态进行研究。【结果】供试广东桑不同品种的花粉微观形态相近，均为小型花粉，近球形，花粉粒极轴长16.98～24.46μm，赤道轴长15.37～23.86μm，极轴长与赤道轴长的比值为1.01～1.12；供试不同品种花粉粒萌发孔1～3个，圆形，直径为2.11～3.51μm，部分花粉粒萌发孔外壁内陷形成漏斗形，按照额尔特曼 G 的 NPC（萌发器官的数目、位置及特征）分类系统属N3P3C4类型；不同倍性间广东桑花粉的赤道轴长、极轴长和萌发孔大小存在显著性差异（P ＜0.05），均表现为四倍体＞二倍体，但花粉粒的极轴/赤轴比值差异不显著（P＞0.05）；广东桑花粉外壁为分布不均的刺状突起－脑条纹复合纹饰，不同品种间花粉的表面纹饰有明显差别，根据外壁纹饰可将供试10个品种花粉分为细刺颗粒状突起－细条纹纹饰、细刺颗粒状突起－粗条纹纹饰、粗刺颗粒状突起－细条纹纹饰和粗刺颗粒状突起－粗条纹纹饰4种类型，花粉外壁纹饰体现了不同品种的遗传多样性和遗传分化；不同倍性间广东桑花粉表面的刺状突起密度及大小差异不显著（P＞0.05）；供试四倍体广东桑品种花粉存在少数败育花粉粒，表现为干瘪而不充实，外形异常。【结论】供试广东桑不同倍性及品种间花粉形态特征具有差异，不同倍性间花粉粒和萌发孔大小存在差异，不同品种间花粉的表面纹饰存在明显差别，初步认为花粉的亚显微结构特征可作为广东桑倍性鉴定及品种间鉴别的重要标准之一。展开更多
基金Supported by Program of National Science and Technology Infra-structure (2005DKA21002-09)~~
文摘[Objective] The study aimed at analyzing the genetic relationship of 64 local varieties of Morus atropurpurea Roxb.from the Pearl River Basin in Guangdong and Guangxi Provinces.[Method] Genetic diversity of 64 local varieties of Morus atropurpurea Roxb.was analyzed by ISSR molecular marker technique.The genetic relationship among these local varieties was researched by UPGMA method based on the genetic similarity coefficient.[Result] 128 bands were amplified from the total DNA of 64 local varieties using 13 ISSR primers,of which 109 bands accounting for 85.15% were polymorphic.It meant that there was rich genetic diversity among the local varieties tested.The genetic similarity coefficients among 64 local varieties were relatively high with a range of 0.500 0-0.929 7.In addition,64 local varieties were divided into two categories and the second could be further divided into 10 subcategories.It was suggested that the genetic relationship of 64 local varieties of Morus atropurpurea Roxb.based on ISSR marker analysis has a certain correlation with geographical distribution.[Conclusion] ISSR marker technology was suitable for evaluating genetic relationship and genetic diversity of local varieties of Morus atropurpurea Roxb.in Pearl River Basin,and could provide scientific basis for DNA fingerprinting and identification of varieties of Morus atropurpurea Roxb.
文摘【目的】针对桑树形态倍性鉴定及品种间鉴别问题，研究广东桑不同倍性及品种之间孢粉学上的异同，为桑树多倍体育种提供科学依据。【方法】利用扫描电镜对广东桑10个不同倍性品种花粉的形态、大小、萌发孔、外壁纹饰等孢粉学特性进行观察，并运用图片分析结合数理统计的方法对其花粉形态进行研究。【结果】供试广东桑不同品种的花粉微观形态相近，均为小型花粉，近球形，花粉粒极轴长16.98～24.46μm，赤道轴长15.37～23.86μm，极轴长与赤道轴长的比值为1.01～1.12；供试不同品种花粉粒萌发孔1～3个，圆形，直径为2.11～3.51μm，部分花粉粒萌发孔外壁内陷形成漏斗形，按照额尔特曼 G 的 NPC（萌发器官的数目、位置及特征）分类系统属N3P3C4类型；不同倍性间广东桑花粉的赤道轴长、极轴长和萌发孔大小存在显著性差异（P ＜0.05），均表现为四倍体＞二倍体，但花粉粒的极轴/赤轴比值差异不显著（P＞0.05）；广东桑花粉外壁为分布不均的刺状突起－脑条纹复合纹饰，不同品种间花粉的表面纹饰有明显差别，根据外壁纹饰可将供试10个品种花粉分为细刺颗粒状突起－细条纹纹饰、细刺颗粒状突起－粗条纹纹饰、粗刺颗粒状突起－细条纹纹饰和粗刺颗粒状突起－粗条纹纹饰4种类型，花粉外壁纹饰体现了不同品种的遗传多样性和遗传分化；不同倍性间广东桑花粉表面的刺状突起密度及大小差异不显著（P＞0.05）；供试四倍体广东桑品种花粉存在少数败育花粉粒，表现为干瘪而不充实，外形异常。【结论】供试广东桑不同倍性及品种间花粉形态特征具有差异，不同倍性间花粉粒和萌发孔大小存在差异，不同品种间花粉的表面纹饰存在明显差别，初步认为花粉的亚显微结构特征可作为广东桑倍性鉴定及品种间鉴别的重要标准之一。
