Korean ginseng and mountain ginseng （Panax ginseng CA Meyer） are important traditional herbal plants whose ginsenosides are generally accepted as serving to improve sexual functions, such as penile erection. We inve...Korean ginseng and mountain ginseng （Panax ginseng CA Meyer） are important traditional herbal plants whose ginsenosides are generally accepted as serving to improve sexual functions, such as penile erection. We investigated the effects of tissue-cultured mountain ginseng extract （TMGE） on male patients with erectile dysfunction （ED）. A double-blind, placebo-controlled study was conducted with 143 patients experiencing ED. Over the course of 8 weeks, one group took 1 000 mg of TMGE twice a day, and the other group took 1 000 mg of placebo twice a day. The effects of the TMGE and the placebo were analyzed using the Korean version of the International Index of Erectile Function （IIEF） questionnaire. A total of 86 patients completed 8 weeks of treatment. The scores on the five domains of the IIEF after medication were significantly higher than the baseline scores in the group treated with TMGE （P 〈 0.05）, whereas no significant improvement was observed in the placebo group （P 〉 0.05）. Erectile function and overall satisfaction scores after medication were significantly higher in the TMGE group than in the placebo group （P 〈 0.05）. Erectile function of patients in the TMGE-treated group significantly improved, suggesting that TMGE could be utilized for improving erectile function in male patients.展开更多
Phellodendron amurense has been used for many years as a medical plant in traditional Chinese medicine and has shown great prospect in recent clinical trials for future applications. Berberine is an essential active c...Phellodendron amurense has been used for many years as a medical plant in traditional Chinese medicine and has shown great prospect in recent clinical trials for future applications. Berberine is an essential active compound contained in P. amurense. Our objective in this study was to quantify the content of berberine in P.amurense from sites at different elevations on Changbai Mountain. We collected samples of P. amurense from five different elevations on Changbai Mountain. Berberine in samples was extracted by ultrahigh pressure extraction（UPE）. And the quantity was measured by high performance liquid chromatography（HPLC）. First, the optimal HPLC conditions for berberine were identified with satisfactory precision（relative standard deviation, R SD／5.6 %）, good accuracy（relative error, R E／ 3.6 %） and good linear relation（R2= 0.9998） in the range of 6.576–328.8 mg L-1. Second, the combination of UPE and HPLC methods in quantitative analysis of berberine showed high repeatability（R SD= 3.28 %）, reproducibility（R SD= 4.72 %）,stability（R SD／ 1.27 %） and good recovery（99.54 %） for real plant materials. Samples from Heilongjiang Province at the lowest elevation contained the highest amount of berberine.Similarly, the lowest amount of berberine was recorded in samples from Changbai Forest Bureau of Jilin Province collected at the highest elevation in this paper. The proposed UPE–HPLC method is simple, reliable and low-cost for quantitative analysis of berberine. Content of berberine in P.amurense varied significantly by site on Changbai Mountain.展开更多
Objective： To investigate the effect of three major ginsenosides from mountain ginseng as anti- cancer substance and explore the underlying mechanism involved in lung cancer. Methods： The inhibitory proliferation of...Objective： To investigate the effect of three major ginsenosides from mountain ginseng as anti- cancer substance and explore the underlying mechanism involved in lung cancer. Methods： The inhibitory proliferation of lung cancer by major five ginsenosides （Rbl, Rb2, Rgl, Rc, and Re） was examined using 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide assay. Calculated 50% inhibition （IC50） values of five ginsenosides were determined and compared each other. Apoptosis by the treatment of single ginsenoside was performed by fluorescence-assisted cytometric spectroscopy. The alterations of apoptosis-related proteins were evaluated by Western blot analysis. Results： The abundance of ginsenosides in butanol extract of mountain ginseng （BX-MG） was revealed in the order of Rbl, Rgl, Re, Rc and Rb2. Among them, Rbl was the most effective to lung cancer cell, followed by Rb2 and Rgl on the basis of relative IC50 values of IMR90 versus A549 cell. The alterations of apoptotic proteins were confirmed in lung cancer A549 cells according to the administration of Rbl, Rb2 and Rgl. The expression levels of caspase-3 and caspase-8 were increased upon the treatment of three ginsenosides, however, the levels of caspase-9 and anti-apoptotic protein Bax were not changed. Conclusion： Major ginsenosides such as Rbl, Rb2 and Rgl comprising BX-MG induced apoptosis in lung cancer cells via extrinsic apoptotic pathway rather than intrinsic mitochondrial pathway.展开更多
文摘Korean ginseng and mountain ginseng （Panax ginseng CA Meyer） are important traditional herbal plants whose ginsenosides are generally accepted as serving to improve sexual functions, such as penile erection. We investigated the effects of tissue-cultured mountain ginseng extract （TMGE） on male patients with erectile dysfunction （ED）. A double-blind, placebo-controlled study was conducted with 143 patients experiencing ED. Over the course of 8 weeks, one group took 1 000 mg of TMGE twice a day, and the other group took 1 000 mg of placebo twice a day. The effects of the TMGE and the placebo were analyzed using the Korean version of the International Index of Erectile Function （IIEF） questionnaire. A total of 86 patients completed 8 weeks of treatment. The scores on the five domains of the IIEF after medication were significantly higher than the baseline scores in the group treated with TMGE （P 〈 0.05）, whereas no significant improvement was observed in the placebo group （P 〉 0.05）. Erectile function and overall satisfaction scores after medication were significantly higher in the TMGE group than in the placebo group （P 〈 0.05）. Erectile function of patients in the TMGE-treated group significantly improved, suggesting that TMGE could be utilized for improving erectile function in male patients.
基金supported by the 12th five-year National Science and Technology plan of China(2012BAC01B03)the 111 Project of China(B13007)
文摘Phellodendron amurense has been used for many years as a medical plant in traditional Chinese medicine and has shown great prospect in recent clinical trials for future applications. Berberine is an essential active compound contained in P. amurense. Our objective in this study was to quantify the content of berberine in P.amurense from sites at different elevations on Changbai Mountain. We collected samples of P. amurense from five different elevations on Changbai Mountain. Berberine in samples was extracted by ultrahigh pressure extraction（UPE）. And the quantity was measured by high performance liquid chromatography（HPLC）. First, the optimal HPLC conditions for berberine were identified with satisfactory precision（relative standard deviation, R SD／5.6 %）, good accuracy（relative error, R E／ 3.6 %） and good linear relation（R2= 0.9998） in the range of 6.576–328.8 mg L-1. Second, the combination of UPE and HPLC methods in quantitative analysis of berberine showed high repeatability（R SD= 3.28 %）, reproducibility（R SD= 4.72 %）,stability（R SD／ 1.27 %） and good recovery（99.54 %） for real plant materials. Samples from Heilongjiang Province at the lowest elevation contained the highest amount of berberine.Similarly, the lowest amount of berberine was recorded in samples from Changbai Forest Bureau of Jilin Province collected at the highest elevation in this paper. The proposed UPE–HPLC method is simple, reliable and low-cost for quantitative analysis of berberine. Content of berberine in P.amurense varied significantly by site on Changbai Mountain.
基金financially supported bythe Korea Basic Science Institute grant(D33403)partly by University-Institute Cooperation Program grant of the National Research Foundation funded by the Korean Government(MEST)
文摘Objective： To investigate the effect of three major ginsenosides from mountain ginseng as anti- cancer substance and explore the underlying mechanism involved in lung cancer. Methods： The inhibitory proliferation of lung cancer by major five ginsenosides （Rbl, Rb2, Rgl, Rc, and Re） was examined using 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide assay. Calculated 50% inhibition （IC50） values of five ginsenosides were determined and compared each other. Apoptosis by the treatment of single ginsenoside was performed by fluorescence-assisted cytometric spectroscopy. The alterations of apoptosis-related proteins were evaluated by Western blot analysis. Results： The abundance of ginsenosides in butanol extract of mountain ginseng （BX-MG） was revealed in the order of Rbl, Rgl, Re, Rc and Rb2. Among them, Rbl was the most effective to lung cancer cell, followed by Rb2 and Rgl on the basis of relative IC50 values of IMR90 versus A549 cell. The alterations of apoptotic proteins were confirmed in lung cancer A549 cells according to the administration of Rbl, Rb2 and Rgl. The expression levels of caspase-3 and caspase-8 were increased upon the treatment of three ginsenosides, however, the levels of caspase-9 and anti-apoptotic protein Bax were not changed. Conclusion： Major ginsenosides such as Rbl, Rb2 and Rgl comprising BX-MG induced apoptosis in lung cancer cells via extrinsic apoptotic pathway rather than intrinsic mitochondrial pathway.
