[Objective] Taqman Quantitative PCR technique was adopted to detect the copies of exogenous nos terminator in transgenic hybrid soybean.[Method] With soybean Lectin as the endogenous reference gene,and gene complex DN...[Objective] Taqman Quantitative PCR technique was adopted to detect the copies of exogenous nos terminator in transgenic hybrid soybean.[Method] With soybean Lectin as the endogenous reference gene,and gene complex DNA in non-GMO soybeans as the endogenous reference standard,the method of gradient dilution was used for separately calculate Ct value of endogenous reference gene and plasmid DNA and correlation standard curve equation of logarithm of copies,and then to calculate the copies of samples through substituting thus-obtained Ct into the standard curve equation.[Result] The standard curve equation of endogenous reference gene is y=-3.422x＋35.201,R2=0.998;and the standard curve equation of exogenous gene is y=-3.348x＋34.890,R2=0.999.Nos terminator and its lower boundary sequences in transgenic soybean is of single copy.[Conclusion] The study has provided a theoretical basis for determining exogenous gene copies in transgenic soybean.展开更多
Let X be a Banach space. If there exists a quotient space of X which is asymptotically isometric to l^1, then X contains complemented asymptotically isometric copies of l^1. Every infinite dimensional closed subspace ...Let X be a Banach space. If there exists a quotient space of X which is asymptotically isometric to l^1, then X contains complemented asymptotically isometric copies of l^1. Every infinite dimensional closed subspace of l1. contains a complemented subspace of l1 which is asymptotically isometric to l1. Let X be a separable Banach space such that X^＊ contains asymptotically isometric copies of lp （1 〈 p 〈∞）. Then there exists a quotient space of X which is asymptotically isometric to lq （1/p ＋ 1/q=1）. Complemented asymptotically isometric copies of co in K（X, Y） and W（X, Y） are discussed. Let X be a Gelfand-Phillips space. If X contains asymptotically isometric copies of co, it has to contain complemented asymptotically isometric copies of co.展开更多
Collecting silver artefacts has traditionally been a very popular hobby.Silver is addictive,therefore the number of potential collectors and investors appears to grow each year.Unfortunately,increases in the interest ...Collecting silver artefacts has traditionally been a very popular hobby.Silver is addictive,therefore the number of potential collectors and investors appears to grow each year.Unfortunately,increases in the interest and buying potentials resulted in a number of forgeries manufactured and introduced to the open antique market.The items such as early silver candlesticks dictate a very high price,for many high quality fakes show very good appearances and matching similarities with originals.Such copies are traditionally manufactured by casting using the original items as patterns.Small details and variances in design features,position and shape of hallmarks,including the final surface quality are usual features to distinguish the fakes from the originals.This paper presents results of a study conducted on several silver candlesticks,including two artefacts bearing features of those produced in the mid 18th century,one original Italian candelabrum from Fascist era,and small candlesticks made in the early 20th century.Also,the paper presents some interesting contemporary coins-replicas of many those produced in different countries.The coins were offered for sale by unscrupulous dealers via auctions and e-bays.Finally the main results and findings from this study are discussed from a manufacturing point of view,such as fabrication technology,surface quality and hallmarks,which will help the collectors,dealers and investors to detect and avoid forgeries.展开更多
目的分析高龄孕妇介入性产前诊断胎儿染色体异常结果的特征。方法回顾性选取2020年1月至2023年6月于唐山市妇幼保健院产前诊断遗传病诊断中心就诊的行羊膜腔穿刺术的638例高龄孕妇作为研究对象,按照孕妇预产年龄分为A组(35~<40岁,n=4...目的分析高龄孕妇介入性产前诊断胎儿染色体异常结果的特征。方法回顾性选取2020年1月至2023年6月于唐山市妇幼保健院产前诊断遗传病诊断中心就诊的行羊膜腔穿刺术的638例高龄孕妇作为研究对象,按照孕妇预产年龄分为A组(35~<40岁,n=463)和B组(≥40岁,n=175),统计2组高龄孕妇羊水细胞染色体核型分析结果和全基因组拷贝数变异测序(copy number variation sequencing,CNV-seq)检测结果。统计学方法采用χ^(2)检验。结果638例高龄孕妇中,羊水细胞染色体异常核型检出率为8.3%(53/638),其中A组和B组的检出率分别为6.9%(32/463)和12.0%(21/175),B组高于A组(χ^(2)=15.241,P<0.05)。CNV-seq检测结果显示,羊水细胞染色体异常拷贝数变异(copy number variation,CNV)检出率为10.2%(65/638),其中A组和B组的检出率分别为8.9%(41/463)和13.7%(24/175),B组高于A组(χ^(2)=13.634,P<0.05)。结论在高龄孕妇中,胎儿染色体异常发生率随着孕妇年龄增长而上升,行产前诊断羊水细胞染色体核型分析及CNV-seq检测可提高胎儿染色体遗传病的检出率。展开更多
基金Funded by Program of Technology Bureau of Harbin(2010RFQXN101)Sub-project of Transgenic Significant Specific Project(2008ZX08012-001)~~
文摘[Objective] Taqman Quantitative PCR technique was adopted to detect the copies of exogenous nos terminator in transgenic hybrid soybean.[Method] With soybean Lectin as the endogenous reference gene,and gene complex DNA in non-GMO soybeans as the endogenous reference standard,the method of gradient dilution was used for separately calculate Ct value of endogenous reference gene and plasmid DNA and correlation standard curve equation of logarithm of copies,and then to calculate the copies of samples through substituting thus-obtained Ct into the standard curve equation.[Result] The standard curve equation of endogenous reference gene is y=-3.422x＋35.201,R2=0.998;and the standard curve equation of exogenous gene is y=-3.348x＋34.890,R2=0.999.Nos terminator and its lower boundary sequences in transgenic soybean is of single copy.[Conclusion] The study has provided a theoretical basis for determining exogenous gene copies in transgenic soybean.
基金Supported by NSFC(10271060)NSFC(10171014) the Doctoral Programme Foundation of Institution of Higher Education(20010055013).
文摘Let X be a Banach space. If there exists a quotient space of X which is asymptotically isometric to l^1, then X contains complemented asymptotically isometric copies of l^1. Every infinite dimensional closed subspace of l1. contains a complemented subspace of l1 which is asymptotically isometric to l1. Let X be a separable Banach space such that X^＊ contains asymptotically isometric copies of lp （1 〈 p 〈∞）. Then there exists a quotient space of X which is asymptotically isometric to lq （1/p ＋ 1/q=1）. Complemented asymptotically isometric copies of co in K（X, Y） and W（X, Y） are discussed. Let X be a Gelfand-Phillips space. If X contains asymptotically isometric copies of co, it has to contain complemented asymptotically isometric copies of co.
文摘Collecting silver artefacts has traditionally been a very popular hobby.Silver is addictive,therefore the number of potential collectors and investors appears to grow each year.Unfortunately,increases in the interest and buying potentials resulted in a number of forgeries manufactured and introduced to the open antique market.The items such as early silver candlesticks dictate a very high price,for many high quality fakes show very good appearances and matching similarities with originals.Such copies are traditionally manufactured by casting using the original items as patterns.Small details and variances in design features,position and shape of hallmarks,including the final surface quality are usual features to distinguish the fakes from the originals.This paper presents results of a study conducted on several silver candlesticks,including two artefacts bearing features of those produced in the mid 18th century,one original Italian candelabrum from Fascist era,and small candlesticks made in the early 20th century.Also,the paper presents some interesting contemporary coins-replicas of many those produced in different countries.The coins were offered for sale by unscrupulous dealers via auctions and e-bays.Finally the main results and findings from this study are discussed from a manufacturing point of view,such as fabrication technology,surface quality and hallmarks,which will help the collectors,dealers and investors to detect and avoid forgeries.
文摘目的分析高龄孕妇介入性产前诊断胎儿染色体异常结果的特征。方法回顾性选取2020年1月至2023年6月于唐山市妇幼保健院产前诊断遗传病诊断中心就诊的行羊膜腔穿刺术的638例高龄孕妇作为研究对象,按照孕妇预产年龄分为A组(35~<40岁,n=463)和B组(≥40岁,n=175),统计2组高龄孕妇羊水细胞染色体核型分析结果和全基因组拷贝数变异测序(copy number variation sequencing,CNV-seq)检测结果。统计学方法采用χ^(2)检验。结果638例高龄孕妇中,羊水细胞染色体异常核型检出率为8.3%(53/638),其中A组和B组的检出率分别为6.9%(32/463)和12.0%(21/175),B组高于A组(χ^(2)=15.241,P<0.05)。CNV-seq检测结果显示,羊水细胞染色体异常拷贝数变异(copy number variation,CNV)检出率为10.2%(65/638),其中A组和B组的检出率分别为8.9%(41/463)和13.7%(24/175),B组高于A组(χ^(2)=13.634,P<0.05)。结论在高龄孕妇中,胎儿染色体异常发生率随着孕妇年龄增长而上升,行产前诊断羊水细胞染色体核型分析及CNV-seq检测可提高胎儿染色体遗传病的检出率。
