This study analyzed the mitochondrial DNA D-loop hypervariable region 601 bp sequence in 12 Eutamias sibiricus from Heilongjiang area. The result showed that the average contents of A, T, G and C were 33.2%, 30.5%, 11...This study analyzed the mitochondrial DNA D-loop hypervariable region 601 bp sequence in 12 Eutamias sibiricus from Heilongjiang area. The result showed that the average contents of A, T, G and C were 33.2%, 30.5%, 11.8% and 24.5% respectively, the A＋T content （63.7%） was obviously higher than the G＋C content （36.3%）. Thirty-six, mutation （approximately 6.0%） sites were found and 9 haplotypes were defined. The mutations types, including transition, transversion and deletion were all found in the detected mtDNA D-loop regions, most of which was transition. The average nucleotide mutational ratio was 1.22%. The nucleotide mutation sites affected the restriction site appearance or disappearance of the restriction site. The research on mtDNA D-loop is focused on the domestic animals and there is no report on Eutamias sibiricus, This study analyzed the mitochondrial DNA D-loop hypervariable in Eutamias sibiricus so as to provide some useful informations for related research in the future.展开更多
Complete mitochondrial D-loop sequences of 140 samples obtained from 15 Chinese local cattle breeds were analyzed.The result showed 67 different haplotypes with 113 polymorphic sites.The haplotype diversity and nucleo...Complete mitochondrial D-loop sequences of 140 samples obtained from 15 Chinese local cattle breeds were analyzed.The result showed 67 different haplotypes with 113 polymorphic sites.The haplotype diversity and nucleotide diversity were 0.608-1.000 and 0.002-0.028,respectively,indicating abundant genetic diversity in Chinese cattle.The constructed Neighbor-Joining(NJ) tree revealed that there were two maternal origins of Bos taurus and Bos indicus in Chinese cattle.Among them,the Bos taurus lineage had rich mtDNA genetic diversity,while Bos indicus lineage had poor mtDNA genetic diversity,respectively.It can be concluded that the cattle in Northern China originated from the Bos taurus lineage,while the cattle in Central China and Southern China originated from Bos taurus and Bos indicus lineages.In our study,two mtDNA haplotypes belonging to African cattle were detected,thus providing molecular evidence for an African lineage in Chinese cattle.展开更多
文摘[目的]研究庆阳驴养殖群体的遗传多样性与母系起源,了解其遗传信息,为保护庆阳驴种质资源、选育和遗传改良工作提供理论依据。[方法]随机选取133头庆阳驴,对其线粒体DNA(mitochondrial DNA,mtDNA)D-loop区序列进行PCR扩增、测序及比对,并探讨庆阳驴的遗传多样性与母系起源。[结果]在获得的520 bp D-loop碱基序列中,AT含量(57.3%)高于GC含量(42.8%),表现出碱基的偏倚性;检测到38个变异位点,包含8个碱基对的转换;其核苷酸多样性(Pi)、单倍型多样性(Hd)、平均核苷酸差异(K)分别为0.01591、0.895和8.274,与欧洲家驴和中国家驴研究的平均值相比较低,说明该驴品种核苷酸变异较为贫乏。庆阳驴mtDNA D-loop区存在35个单倍型,单倍型之间的遗传距离为0.002~0.042。系统进化结果显示,庆阳驴存在2个线粒体支系,表明其具有2个母系起源,且遗传距离表明,庆阳驴与克罗地亚家驴之间的遗传距离较近。[结论]本研究从分子水平初步揭示庆阳驴核苷酸变异比较贫乏,杂交程度高,mtDNA遗传多态性正逐步丧失,应加强庆阳驴品种的遗传资源保护工作。
文摘This study analyzed the mitochondrial DNA D-loop hypervariable region 601 bp sequence in 12 Eutamias sibiricus from Heilongjiang area. The result showed that the average contents of A, T, G and C were 33.2%, 30.5%, 11.8% and 24.5% respectively, the A＋T content （63.7%） was obviously higher than the G＋C content （36.3%）. Thirty-six, mutation （approximately 6.0%） sites were found and 9 haplotypes were defined. The mutations types, including transition, transversion and deletion were all found in the detected mtDNA D-loop regions, most of which was transition. The average nucleotide mutational ratio was 1.22%. The nucleotide mutation sites affected the restriction site appearance or disappearance of the restriction site. The research on mtDNA D-loop is focused on the domestic animals and there is no report on Eutamias sibiricus, This study analyzed the mitochondrial DNA D-loop hypervariable in Eutamias sibiricus so as to provide some useful informations for related research in the future.
文摘Complete mitochondrial D-loop sequences of 140 samples obtained from 15 Chinese local cattle breeds were analyzed.The result showed 67 different haplotypes with 113 polymorphic sites.The haplotype diversity and nucleotide diversity were 0.608-1.000 and 0.002-0.028,respectively,indicating abundant genetic diversity in Chinese cattle.The constructed Neighbor-Joining(NJ) tree revealed that there were two maternal origins of Bos taurus and Bos indicus in Chinese cattle.Among them,the Bos taurus lineage had rich mtDNA genetic diversity,while Bos indicus lineage had poor mtDNA genetic diversity,respectively.It can be concluded that the cattle in Northern China originated from the Bos taurus lineage,while the cattle in Central China and Southern China originated from Bos taurus and Bos indicus lineages.In our study,two mtDNA haplotypes belonging to African cattle were detected,thus providing molecular evidence for an African lineage in Chinese cattle.
文摘为更好地对长顺绿壳蛋鸡进行品种保护和利用,并从母系遗传角度深入阐明长顺绿壳蛋鸡的群体遗传背景,利用PCR直接测序法,测定了114只长顺绿壳蛋鸡的线粒体DNA(mt DNA)D-loop第1高变区568 bp片段序列。结果表明:在所分析的长顺绿壳蛋鸡mt DNA D-loop区序列中,A、C、G、T平均比例为27.1%、29.5%、13.4%、30.0%;共检测到核苷酸变异位点37个,核苷酸多样度为0.0111,24种单倍型,单倍型多样度为0.845,表明长顺绿壳蛋鸡遗传多样性较丰富,具有较好的选育潜力。聚类分析结果表明,长顺绿壳蛋鸡与红原鸡亲缘关系较近。
