Aim： To examine whether a relationship exists between glutathione S-transferase Mu-1 （GSTM1） gene polymorphism and the susceptibility of sperm and seminal plasma from patients with idiopathic infertility to oxidati...Aim： To examine whether a relationship exists between glutathione S-transferase Mu-1 （GSTM1） gene polymorphism and the susceptibility of sperm and seminal plasma from patients with idiopathic infertility to oxidative stress. Methods： Fifty-two men with idiopathic infertility and 60 healthy fertile men were recruited to this study. GSTM1 gene polymorphism was determined by polymerase chain reaction （PCR） and both the infertile and control individuals were divided into GSTM1 null and GSTM1 positive groups according to their GSTM1 gene structure. We compared reactive oxygen species （ROS） generation, malondialdehyde （MDA）, protein carbonyls and glutathione （GSH） concentrations, and glutathione S-transferase （GST） activity in seminal plasma and spermatozoa from infertile patients and controls with respect to GSTM1 genotype. Results： Significantly higher levels of oxidative stress and damage markers were found in idiopathic infertile men with the GSTM1 null genotype compared with those with the GSTM1 positive genotype. There was no significant difference in genotype distribution for theGSTM1 variant between the idiopathic infertile subjects and fertile subjects. Patients with the GSTM1 null genotype also had lower sperm concentrations than those with GSTM1 positive genotype. Conclusion： Our results suggest that the susceptibility of sperm and seminal plasma to oxidative stress is significantly greater in idiopathic infertile men with the GSTM1 null genotype compared with those possessing the gene. Therefore, in patients with idiopathic infertility, GSTM1 polymorphism might be an important source of variation in susceptibility of spermatozoa to oxidative damage.展开更多
胰岛素样生长因子I是1种具有促进动物生长发育和繁殖的的肽类物质。动物要达到正常的性成熟和生殖能力则需要适当的IGF-Ⅰ水平。睾丸是雄性动物的重要生殖器官,为了探讨IGF-Ⅰ基因对于雄性动物睾丸组织发育的影响,本实验采用Real Time R...胰岛素样生长因子I是1种具有促进动物生长发育和繁殖的的肽类物质。动物要达到正常的性成熟和生殖能力则需要适当的IGF-Ⅰ水平。睾丸是雄性动物的重要生殖器官,为了探讨IGF-Ⅰ基因对于雄性动物睾丸组织发育的影响,本实验采用Real Time RT-PCR方法研究了IGF-Ⅰ基因在军牧1号白猪不同发育阶段睾丸组织的表达规律。结果表明:IGF-Ⅰ在不同发育阶段的睾丸组织中均表达,但是各个不同发育时期的表达量差异显著,六月龄时(性成熟阶段)表达量最高。本研究为深入探讨IGF-I基因调控动物繁殖机能以及在生产中如何提高家猪的繁殖力奠定了分子生物学基础。展开更多
AIM: Glutathione S-transferase mu 1 (GSTM1) and theta 1(GSTT1) genes are involved in the metabolism of a wide range of carcinogens, but deletions of the genes are commonly found in the population. The present study wa...AIM: Glutathione S-transferase mu 1 (GSTM1) and theta 1(GSTT1) genes are involved in the metabolism of a wide range of carcinogens, but deletions of the genes are commonly found in the population. The present study was undertaken to evaluate the association between GSTM1 and GSTT1 gene polymorphisms and hepatocellular carcinoma (HCC) risk.METHODS: The genetic polymorphisms were studied at an aflatoxin highly contaminated region in Guangxi, China.Polymerase chain reaction (PCR) technique was used to detect the presence or absence of the GSTM1 and GSTT1 genes in blood samples. The case group was composed of 181 patients of HCC identified by the pathologists and the control group was composed of 360 adults without any tumor.RFSULTS: The frequencies of GSTM1 and GSTT1 null genotypes in the control were 47.8% and 42.7%, while those in the HCC group were 64.6% and 59.7%, respectively. The differences between HCC group and control group were very significant (P＜0.01). GSTM1 and GSTT1 combined null genotypes in HCC group and control group were 38.2% and 18.5%respectively, and the difference was significant (P＜0.05).CONCLUSION: The GSTM1 and GSTT1 null genotypes are associated with an increased risk of HCC in a special geographic environment. Combination of the two null genotypes in an individual is substantially increased twice the risk of HCC.展开更多
文摘Aim： To examine whether a relationship exists between glutathione S-transferase Mu-1 （GSTM1） gene polymorphism and the susceptibility of sperm and seminal plasma from patients with idiopathic infertility to oxidative stress. Methods： Fifty-two men with idiopathic infertility and 60 healthy fertile men were recruited to this study. GSTM1 gene polymorphism was determined by polymerase chain reaction （PCR） and both the infertile and control individuals were divided into GSTM1 null and GSTM1 positive groups according to their GSTM1 gene structure. We compared reactive oxygen species （ROS） generation, malondialdehyde （MDA）, protein carbonyls and glutathione （GSH） concentrations, and glutathione S-transferase （GST） activity in seminal plasma and spermatozoa from infertile patients and controls with respect to GSTM1 genotype. Results： Significantly higher levels of oxidative stress and damage markers were found in idiopathic infertile men with the GSTM1 null genotype compared with those with the GSTM1 positive genotype. There was no significant difference in genotype distribution for theGSTM1 variant between the idiopathic infertile subjects and fertile subjects. Patients with the GSTM1 null genotype also had lower sperm concentrations than those with GSTM1 positive genotype. Conclusion： Our results suggest that the susceptibility of sperm and seminal plasma to oxidative stress is significantly greater in idiopathic infertile men with the GSTM1 null genotype compared with those possessing the gene. Therefore, in patients with idiopathic infertility, GSTM1 polymorphism might be an important source of variation in susceptibility of spermatozoa to oxidative damage.
文摘胰岛素样生长因子I是1种具有促进动物生长发育和繁殖的的肽类物质。动物要达到正常的性成熟和生殖能力则需要适当的IGF-Ⅰ水平。睾丸是雄性动物的重要生殖器官,为了探讨IGF-Ⅰ基因对于雄性动物睾丸组织发育的影响,本实验采用Real Time RT-PCR方法研究了IGF-Ⅰ基因在军牧1号白猪不同发育阶段睾丸组织的表达规律。结果表明:IGF-Ⅰ在不同发育阶段的睾丸组织中均表达,但是各个不同发育时期的表达量差异显著,六月龄时(性成熟阶段)表达量最高。本研究为深入探讨IGF-I基因调控动物繁殖机能以及在生产中如何提高家猪的繁殖力奠定了分子生物学基础。
基金Supported by The Natural Scientific Foundation of China No. 39860032by the Education Department of Guangxi Zhuang Autonomous Region No. 98-2-8
文摘AIM: Glutathione S-transferase mu 1 (GSTM1) and theta 1(GSTT1) genes are involved in the metabolism of a wide range of carcinogens, but deletions of the genes are commonly found in the population. The present study was undertaken to evaluate the association between GSTM1 and GSTT1 gene polymorphisms and hepatocellular carcinoma (HCC) risk.METHODS: The genetic polymorphisms were studied at an aflatoxin highly contaminated region in Guangxi, China.Polymerase chain reaction (PCR) technique was used to detect the presence or absence of the GSTM1 and GSTT1 genes in blood samples. The case group was composed of 181 patients of HCC identified by the pathologists and the control group was composed of 360 adults without any tumor.RFSULTS: The frequencies of GSTM1 and GSTT1 null genotypes in the control were 47.8% and 42.7%, while those in the HCC group were 64.6% and 59.7%, respectively. The differences between HCC group and control group were very significant (P＜0.01). GSTM1 and GSTT1 combined null genotypes in HCC group and control group were 38.2% and 18.5%respectively, and the difference was significant (P＜0.05).CONCLUSION: The GSTM1 and GSTT1 null genotypes are associated with an increased risk of HCC in a special geographic environment. Combination of the two null genotypes in an individual is substantially increased twice the risk of HCC.
